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Gene Dmel\CG8461

General Information
Symbol	Dmel\CG8461	Species	D. melanogaster
Name		Annotation symbol	CG8461
Feature type	protein_coding_gene	FlyBase ID	FBgn0038235
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88C3-88C3	Sequence location	3R:10,338,358..10,339,359 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG8461 (FBgn0038235). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: neurogenesis. 3 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein of unknown function DUF947. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1637951_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG8461. Gene sequence location is 3R:10338358..10339359.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88C3-88C3   Limits computationally determined from genome sequence between P{PZ}cv-c06951 and P{PZ}put10460
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG8461 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.48
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG8461-RA FBtr0082986  NM_142110   886   244
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG8461-PA   FBpp0082445   28.7   244   10.68     NP_650367   AAF55064
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein of unknown function DUF947 (IPR009292)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA952190   AE014297 AAF55064   AW942951   AY061371 AAL28919   CO262771   CO303433   CO320666   EC078748   EC088573   EC231112   EC231113   EV595780   EV596161   GH942045   GH944763   GH945270   GH956377   GH960512   GH961877   GH963235   GH964663   GH967919   GH969715   GH971060   GH973614   GH975560   GH977324   GH978101   GH979707   GH981671   GH983133
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VFJ1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) fat body primordium   (Fisher et al., 2012) Comment:reported as fat body specific anlage organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    94.5 Larval Midgut    30.9 Larval Hindgut    42 Larval Malpighian Tubules    40.4 Larval Fat Body    52.7 Larval Salivary Gland    40.4 Larval Trachea    86.35 Larval Carcass    42.85 Adult Head    51.5 Adult Eye    58.875 Adult Brain    76.8 Adult Thoracic-Abdominal Ganglion    68.5 Adult Crop    43.9 Adult Midgut    39.1 Adult Hindgut    38.6 Adult Malpighian Tubules    42.1 Adult Fat Body    51.9 Adult Salivary Gland    99.4 Adult Heart    43.625 Adult VirginFemale Spermatheca    75 Adult InseminatedFemale Spermatheca    94.3 Adult Ovary    149.5 Adult Testis    59.4 Adult Male Accessory Gland    75.5 Adult Carcass    47 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    51.5   NA  Eye    58.875   NA  Brain    76.8   94.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    68.5   NA  Crop    43.9   30.9  Midgut    39.1   42  Hindgut    38.6   40.4  Malpighian Tubules    42.1   52.7  Fat Body    51.9   40.4  Salivary Gland    99.4   NA  Heart    43.625   86.35  Trachea    NA   NA  VirginFemale Spermatheca    75   NA  InseminatedFemale Spermatheca    94.3   NA  Ovary    149.5   NA  Testis    59.4   NA  Male Accessory Gland    75.5   42.85  Carcass    47 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  (30) central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female  (49) ovary, mated 4-day female  (56) testis, mated 4-day male  (36) accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female  (49) ovary, mated 4-day female  (56) testis, mated 4-day male  (36) accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    30 central nervous system, pupae P8    20 head, virgin 1-day female    17 head, virgin 4-day female    18 head, virgin 20-day female    5 head, mated 1-day female    17 head, mated 4-day female    12 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    15 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    8 digestive system, 4-day adult    12 digestive system, 20-day adult    7 fat body, larvae L3 wandering    5 fat body, white prepupae    16 fat body, pupae P8    13 carcass, larvae L3 wandering    17 carcass, 1-day adult    10 carcass, 4-day adult    16 carcass, 20-day adult    11 ovary, virgin 4-day female    49 ovary, mated 4-day female    56 testis, mated 4-day male    36 accessory gland, mated 4-day male    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    13 embryo 02-04hr    25 embryo 04-06hr    24 embryo 06-08hr    17 embryo 08-10hr    18 embryo 10-12hr    17 embryo 12-14hr    16 embryo 14-16hr    4 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    11 embryo 22-24hr    13 larva L1    6 larva L2    6 larva L3 12hr old    4 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    7 white prepupae new    6 white prepupae 12hr    6 white prepupae 24hr    6 pupae 2d postWPP    6 pupae 3d postWPP    5 pupae 4d postWPP    1 adult male 01day    3 adult male 05day    4 adult male 30day    2 adult female 01day    3 adult female 05day    12 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4  (28) embryonic 1182-4H    21 embryonic GM2  (35) embryonic Kc167  (27) embryonic S1    16 embryonic S3  (29) leg disc CME L1    25 wing disc CME-W2  26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21  (32) wing disc ML-DmD32  (39) haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1  (31) CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS  26 ovary OSC  (44) ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  26 Schneider line 2 Sg4  28 embryonic 1182-4H    21 embryonic GM2  (35) embryonic Kc167  27 embryonic S1    16 embryonic S3  29 leg disc CME L1    25 wing disc CME-W2  26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21  32 wing disc ML-DmD32  (39) haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1  31 CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS  26 ovary OSC  (44) ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    26 Schneider line 2 Sg4    28 embryonic 1182-4H    21 embryonic GM2    35 embryonic Kc167    27 embryonic S1    16 embryonic S3    29 leg disc CME L1    25 wing disc CME-W2    26 wing disc ML-DmD8    13 wing disc ML-DmD9    11 wing disc ML-DmD16-c3    22 wing disc ML-DmD21    32 wing disc ML-DmD32    39 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    21 ovary fGS/OSS    26 ovary OSC    44 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult  (43) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult  (31) Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (44) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (28) Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult  (43) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (50) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult  31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (44) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    14 heat shock, 4-day adult    13 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    43 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    50 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    31 Ethanol 2.5% 3 hrs, larvae L3    16 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    44 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    28 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • LD29276 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038235 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8461-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for CG8461 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal CG8461GD13931 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 CG8461GD13931 Other Phenotypes Allele neuroanatomy defective | larval stage CG8461GD13931 visible, with Scer\GAL4pnr-MD237 CG8461GD13931 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage CG8461GD13931 ganglion mother cell | larval stage CG8461GD13931 mesothoracic tergum, with Scer\GAL4pnr-MD237 CG8461GD13931 trichogen cell, with Scer\GAL4pnr-MD237 CG8461GD13931
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of CG8461 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG8461 Class Mutagen Stocks Known lesion CG8461GD13931 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG8461KK102824 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG8461NIG.8461R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13931} NA P{KK102824} NA P{NIG.8461R} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CG8461 - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8461 - Nop56 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8461 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8461 - U3-55K anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CG8461 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG8461 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66833 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0038235 DroID - A comprehensive database of gene and protein interactions. • FBgn0038235 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41758
Orthologs
OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG670V3Q Dipteran inclusive ortholog search EOG65DWZM Insect inclusive ortholog search EOG6JQ2V7 Arthropod inclusive ortholog search EOG6RXWGT Metazoa inclusive ortholog search EOG6T7MZD
Orthologs in Drosophila Species (EOG670V3Q)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG8461     Drosophila simulans   Dsim\GD20416 Y   Drosophila sechellia   Dsec\GM25849 Y   Drosophila erecta   Dere\GG21274 Y   Drosophila yakuba   Dyak\GE26446 Y   Drosophila ananassae   Dana\GF17479 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21095 Y   Drosophila persimilis   Dper\GL23657 Y   Drosophila willistoni   Dwil\GK12146 Y   Drosophila virilis   Dvir\GJ22776 Y   Drosophila mojavensis   Dmoj\GI22774 Y   Drosophila grimshawi   Dgri\GH17448 Y
Orthologs in non-Drosophila Dipterans (EOG65DWZM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Anopheles gambiae Malaria mosquito  Agam\AGAP002333   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012967   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013674   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000064   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013409   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013238
Orthologs in non-Dipteran Insects (EOG6JQ2V7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC724432   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012297   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH25666   Atta cephalotes Leafcutter ant  Acep\ACEP19308   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12959   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21623   Linepithema humile Argentine ant  Lhum\LH20447   Linepithema humile Argentine ant  Lhum\LH13424   Linepithema humile Argentine ant  Lhum\LH14417   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20694   Solenopsis invicta Red fire ant  Sinv\SINV16254   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006912   Bombyx mori Silkmoth  Bmor\BGIBMGA005118   Pediculus humanus Human body louse  Phum\PHUM517330   Tribolium castaneum Red flour beetle  Tcas\TC008830
Orthologs in non-Insect Arthropods (EOG6RXWGT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\adk   Ixodes scapularis Deer tick  Isca\ISCW021586
Orthologs in non-Arthropod Metazoa (EOG6T7MZD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\T22H9.1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006684gn   Danio rerio Zebrafish  Drer\si:dkey-236e20.6   Mus musculus House mouse  Mmus\Rrp36   Rattus norvegicus Norway rat  Rnor\LOC100360664   Homo sapiens Human  Hsap\RRP36
Human Orthologs (1)
Gene OMIM HGNC Hsap\RRP36
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20416 Drosophila sechellia Dsec\GM25849 Drosophila erecta Dere\GG21274 Drosophila yakuba Dyak\GE26446 Drosophila ananassae Dana\GF17479 Drosophila pseudoobscura pseudoobscura Dpse\GA21095 Drosophila persimilis Dper\GL23657 Drosophila willistoni Dwil\GK12146 Drosophila virilis Dvir\GJ22776 Drosophila mojavensis Dmoj\GI22774 Drosophila grimshawi Dgri\GH17448
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v107284 P{KK102824}VIE-260B v24103 w1118; P{GD13931}v24103/CyO
Genomic Clones ( 1 )
	BACR04O02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 28 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD29276
Other clones	DPiM_LD29276
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	121374 182754 CK01.102C.G5 Dmel_neb_011657_0635_2125 Dmel_neb_014202_0585_1741 EK086327 EK241440 EK286859 RP004080278 RP004213247 RP004228772 RP004231981 RP004237452 RP004247425 RP004259914 RP004264290 RP004295549 RP004339295 RP004378541 RP004418517 RP004437118 RP004460973 RP004501035 RP004516422 RP004530988 RP004544517
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038235 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41758
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142110 RefSeq (Proteins) • NP_650367 DDBJ / EMBL / GenBank • AA952190 AAF55064 AAL28919 AW942951 AY061371 Entrez Gene - A searchable database of RefSeq genes. • 41758 UniProtKB/TrEMBL • Q9VFJ1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein of unknown function DUF947 (IPR009292) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD29276
Linkouts
	BioGRID - A database of protein and genetic interactions • 66833 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0038235 DroID - A comprehensive database of gene and protein interactions. • FBgn0038235 DRSC - Results from RNAi screens. • FBgn0038235 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038235 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8461-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038235 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41758 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41758 modMine - Data generated by the modENCODE project. • FBgn0038235
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	CG8461 (Keleman et al., 2009.8.5, Neumüller et al., 2011, Japanese National Institute of Genetics, 2012.5.21)
Name Synonym
Secondary FlyBase IDs
References ( 13 )
Research paper	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] Neumüller et al., 2011, Cell Stem Cell 8(5): Supplemental Table S1. [FBrf0214431] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]