Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Homodimer, and heterodimer; with Gyc-89Db and Gyc-89Da, in the presence of magnesium or manganese.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gyc88E using the Feature Mapper tool.
Gyc88E transcript is expressed at a higher level in adults than in larvae. Both Gyc88E-RA (Gyc-88E-S) and Gyc88E-RC (Gyc-88E-L) are expressed at both stages. Embryonic expression is seen the brain, and in a segmentally repeated pattern in the ventral nerve cord starting at stage 15 or 16. Expression is observed in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segmentsa; embryonic PNS expression colocalizes with that of Gyc-89Db. In the third instar larva, expression is observed in scattered cells throughout the brain, but most prominently in a small cluster of cells in the anterior medial region of each brain lobe. Larval expression in the ventral ganglion is observed in scattered cells located laterally and at the midline.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Gyc88E in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Gyc-89Db shows no guanylyl cyclase activity when expressed in COS-7 cells in the absence of additional subunits, in the presence of either Mg2+ or Mn2+ cofactor. However, when Gyc-89Db and Gyc88E are co-expressed in COS-7 cells, the level of guanylyl cyclase activity, in the presence of either Mg2+ or Mn2+, is higher than when Gyc88E is expressed alone.
COS-7 cells co-expressing Gyc88E and Gyc-89Da or Gyc88E and Gyc-89Db accumulate low levels of cGMP under normal atmospheric oxygen conditions and are potently activated under anoxic conditions, suggesting that the Gyc88E/Gyc-89Da and Gyc88E/Gyc-89Db protein heterodimers may act as oxygen sensors.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.