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Gene Dmel\Gyc88E

General Information
Symbol	Dmel\Gyc88E	Species	D. melanogaster
Name	Guanylyl cyclase at 88E	Annotation symbol	CG4154
Feature type	protein_coding_gene	FlyBase ID	FBgn0038295
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	Gyc-88E
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88E2-88E2	Sequence location	3R:11,011,773..11,016,964 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Guanylyl cyclase at 88E is referred to in FlyBase by the symbol Dmel\Gyc88E (CG4154, FBgn0038295). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: guanylate cyclase activity; carbon monoxide binding; oxygen sensor activity; oxygen binding; heme binding; protein homodimerization activity; nitric oxide binding. There is experimental evidence that it is involved in the biological process: response to hypoxia; cGMP biosynthetic process. 6 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Adenylyl cyclase class-3/4/guanylyl cyclase; Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site; Haem NO binding associated; Heme-NO binding; NO signalling/Golgi transport ligand-binding domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1629608_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Gyc88E. Gene sequence location is 3R:11011773..11016964.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN24528
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88E2-88E2   Limits computationally determined from genome sequence between P{EP}CG33967EP666 and P{lacW}Hsc70-4L3929
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Gyc88E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Extent and structure of 3' and 5' UTRs is undetermined
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Gyc88E-RA FBtr0083027  NM_169616   2873   940 Gyc88E-RC FBtr0083028  NM_142167   2894   947 Gyc88E-RD FBtr0110961  NM_001043246   4399   1097 Gyc88E-RE FBtr0306245     3494   1097
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Gyc88E-PA   FBpp0082486   104.3   940   6.62     NP_731974   AAF55135 Gyc88E-PC   FBpp0082487   105.0   947   6.77     NP_650424   AAN13628 Gyc88E-PD   FBpp0110261   121.2   1097   7.29     NP_001036711   ABI31167 Gyc88E-PE   FBpp0297355   121.2   1097   7.29
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site (IPR018297) Adenylyl cyclase class-3/4/guanylyl cyclase (IPR001054) Heme-NO binding (IPR011644) NO signalling/Golgi transport ligand-binding domain (IPR024096) Haem NO binding associated (IPR011645)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF55135   AE014297 AAN13628   AE014297 ABI31167   AY376404 AAQ84038   AY376405 AAQ84039   BG639921   BI636239   BT029123   BT029937   BT030432   CO186629   CO288463   CO290518   CO290805   CO295495   CO341129   CO345137   EC054799   EC055829   EC058900   EC080259   EC207887   EC210600   EC219004   EC224475   EL886829   EL886887   EL886956   EL887016   EL887081   EL887083   EL887142   EL887143   EL887202   EL887204   EL887257
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8INF0 Q0KI72
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 -- 17   ventral nerve cord | segmentally repeated   (Langlais et al., 2004) embryonic brain   (Langlais et al., 2004) embryonic stage 17   peripheral nervous system | segmentally repeated   (Langlais et al., 2004) neuron of sensillum basiconicum | subset   (Langlais et al., 2004) neuron of external sensory organ | subset   (Langlais et al., 2004) third instar larval stage   brain   (Langlais et al., 2004) ventral nerve cord   (Langlais et al., 2004) northern blot Stage Tissue/Position (including subcellular localization) Reference larval stage -- adult stage   organism   (Langlais et al., 2004) RT-PCR Stage Tissue/Position (including subcellular localization) Reference larval stage -- adult stage   organism   (Langlais et al., 2004) Comment:both Gyc88E-RA and Gyc88E-RC transcripts
Additional Descriptive Data
	Gyc88E transcript is expressed at a higher level in adults than in larvae. Both Gyc88E-RA (Gyc-88E-S) and Gyc88E-RC (Gyc-88E-L) are expressed at both stages. Embryonic expression is seen the brain, and in a segmentally repeated pattern in the ventral nerve cord starting at stage 15 or 16. Expression is observed in several peripheral neurons that innervate trachea, basiconical sensilla and the sensory cones in the posterior segmentsa; embryonic PNS expression colocalizes with that of Gyc-89Db. In the third instar larva, expression is observed in scattered cells throughout the brain, but most prominently in a small cluster of cells in the anterior medial region of each brain lobe. Larval expression in the ventral ganglion is observed in scattered cells located laterally and at the midline. (Langlais et al., 2004)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0038295    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0038295 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of low expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0038295 expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr  (145) embryo 16-18hr  (234) embryo 18-20hr  (339) embryo 20-22hr  (309) embryo 22-24hr  (331) larva L1  (248) larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP  (110) pupae 4d postWPP  (173) adult male 01day  (165) adult male 05day  (191) adult male 30day  (151) adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0038295 expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr  (145) embryo 16-18hr  (234) embryo 18-20hr  (339) embryo 20-22hr  (309) embryo 22-24hr  (331) larva L1  (248) larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP  110 pupae 4d postWPP  (173) adult male 01day  (165) adult male 05day  (191) adult male 30day  (151) adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    2 embryo 08-10hr    4 embryo 10-12hr    9 embryo 12-14hr    23 embryo 14-16hr    145 embryo 16-18hr    234 embryo 18-20hr    339 embryo 20-22hr    309 embryo 22-24hr    331 larva L1    248 larva L2    71 larva L3 12hr old    35 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    20 white prepupae new    15 white prepupae 12hr    31 white prepupae 24hr    37 pupae 2d postWPP    60 pupae 3d postWPP    110 pupae 4d postWPP    173 adult male 01day    165 adult male 05day    191 adult male 30day    151 adult female 01day    66 adult female 05day    22 adult female 30day    21 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0038295    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0038295 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0038295 expression level Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0038295 expression level Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    5.65 Larval Midgut    5.6 Larval Hindgut    2.4 Larval Malpighian Tubules    1.4 Larval Fat Body    2.2 Larval Salivary Gland    3.4 Larval Trachea    0.9 Larval Carcass    1.775 Adult Head no informative data Adult Eye    4.5 Adult Brain    27 Adult Thoracic-Abdominal Ganglion    26.7 Adult Crop    7.2 Adult Midgut    3.4 Adult Hindgut    4 Adult Malpighian Tubules    2.1 Adult Fat Body    7.1 Adult Salivary Gland    5 Adult Heart    1.875 Adult VirginFemale Spermatheca    3.5 Adult InseminatedFemale Spermatheca    6.3 Adult Ovary    2.5 Adult Testis    8.5 Adult Male Accessory Gland    4.2 Adult Carcass    6.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head no informative data Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    4.5   NA  Brain    27   5.65  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    26.7   NA  Crop    7.2   5.6  Midgut    3.4   2.4  Hindgut    4   1.4  Malpighian Tubules    2.1   2.2  Fat Body    7.1   3.4  Salivary Gland    5   NA  Heart    1.875   0.9  Trachea    NA   NA  VirginFemale Spermatheca    3.5   NA  InseminatedFemale Spermatheca    6.3   NA  Ovary    2.5   NA  Testis    8.5   NA  Male Accessory Gland    4.2   1.775  Carcass    6.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_14
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038295 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4154-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4Gyc-89Da.1.9 Gyc88EGD11230 viable, with Scer\GAL4Gyc-89Db.3.4 Gyc88EGD11230 viable, with Scer\GAL4pnr-MD237 Gyc88EGD11230 Other Phenotypes Allele stress response defective | third instar larval stage Gyc88ES451F Gyc88EV474M stress response defective | third instar larval stage, with Scer\GAL4Gyc-89Da.1.9 Gyc88EGD11230 stress response defective | third instar larval stage, with Scer\GAL4Gyc-89Db.3.4 Gyc88EGD11230 stress response defective | third instar larval stage (with Gyc88ES451F) Gyc88EV474M stress response defective | third instar larval stage (with Gyc88EV474M) Gyc88ES451F taste perception defective, with Scer\GAL4Gyc-89Da.1.9 Gyc88EGD11230 taste perception defective, with Scer\GAL4Gyc-89Db.3.4 Gyc88EGD11230 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Gyc88E Class Mutagen Stocks Known lesion Gyc88ES451F 0 Yes Gyc88EV474M 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Gyc88E Class Mutagen Stocks Known lesion Gyc88EGD11230 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Gyc88EHM05096 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gyc88EKK105392 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Gyc88EScer\UAS.cVa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11230} NA P{KK105392} NA P{TRiP.HM05096} NA P{UAS-Gyc88E.V} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
carbon monoxide binding inferred from direct assay (Huang et al., 2007) guanylate cyclase activity inferred from direct assay (Langlais et al., 2004, Huang et al., 2007) heme binding inferred from direct assay (Huang et al., 2007) nitric oxide binding inferred from direct assay (Huang et al., 2007) oxygen binding inferred from direct assay (Huang et al., 2007) oxygen sensor activity inferred from direct assay (Huang et al., 2007) protein homodimerization activity inferred from physical interaction with Gyc88E (Huang et al., 2007)
Biological Process
CV term Evidence References
cGMP biosynthetic process inferred from direct assay (Huang et al., 2007) response to hypoxia inferred from mutant phenotype (Vermehren-Schmaedick et al., 2010)
Cellular Component
CV term Evidence References
guanylate cyclase complex, soluble inferred from direct assay (Huang et al., 2007)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
guanylate cyclase activity inferred from sequence or structural similarity with EMBL:AF064514 (FlyBase, 1992-) inferred from sequence or structural similarity with EMBL:AF064514 (Mi et al., 2001.12.13) non-traceable author statement (Langlais, 2003.8.28, Morrison et al., 2000)
Biological Process
CV term Evidence References
cGMP biosynthetic process inferred by curator from GO:0004383 (Langlais et al., 2004) intracellular signal transduction inferred from electronic annotation with InterPro:IPR001054, InterPro:IPR018297 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
guanylate cyclase complex, soluble inferred from sequence or structural similarity (Langlais et al., 2004) inferred from sequence or structural similarity with EMBL:AF064514 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Gyc88E allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0038295
Orthologs
Genome-wide drosophilid orthologs	Dana\GF17835 Dere\GG16891 Dgri\GH17385 Dmoj\GI23930 Dper\GL22138 Dpse\GA27389 Dsec\GM24200 Dsim\GD18990 Dvir\GJ24025 Dwil\GK13300 Dyak\GE24273
Curated drosophilid orthologs	Dpse\GA17992
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL013328-PA Aedes aegypti (mosquito) AGAP001985-PA Anopheles gambiae (mosquito) NP_001012645.1 Apis mellifera (honey bee) XP_001945138.1 Acyrthosiphon pisum (pea aphid) 268816 Branchiostoma floridae (lancelet) BGIBMGA010713-PA Bombyx mori (silk worm) CN24182 Caenorhabditis brenneri (small nematode) CBP31875 Caenorhabditis briggsae (nematode) CE36319 Caenorhabditis elegans (worm) ENSCINP00000023677 Ciona intestinalis (sea squirt) JA02029 Caenorhabditis japonica (nematode) CPIJ006958 Culex pipens (mosquito) RP30638 Caenorhabditis remanei (nematode) ENSCSAVP00000009053 Ciona savignyi (sedentary tunicate) 91580 Capitella spl (polychaete worm) 308690 Daphnia pulex (daphnia) ENSGACP00000023822 Gymnopilus aculeatus (mushroom) 104058 Lottia gigantea (owl limpet) 33782 Monocosta brevicollis (choanoflagellate) 37136 Monocosta brevicollis (choanoflagellate) 98823 Nematostella vectensis (starlet anemone) XP_001605361.1 Nasonia vitripennis (wasp) PHUM001768-PA Pediculus humanus (human body louse) PP02100 Pristionchus pacificus (nematode) Smp_149980 Schistosoma mansoni (trematode parasite) XP_001197014.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001197293.1 Strongylocentrotus purpuratus (purple sea urchin) XP_971396.2 Tribolium castaneum (red flour beetle) ENSTNIP00000008913 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000010396 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15627 LH16225 ISCW017630 FBgn0241402 FBgn0215309 FBgn0211112 FBgn0190499 FBgn0179064 FBgn0248756 FBgn0159730 FBgn0146655 FBgn0124855 FBgn0109119 FBgn0094853 FBgn0090877 CPIJ006958 BGIBMGA010713 ACYPI009560 GB18735 AGAP001985 AAEL013328 PB25604 PHUM008080 GLEAN_08423
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	28608 y1 v1; P{TRiP.HM05096}attP2
VDRC	v101672 P{KK105392}VIE-260B v21797 w1118; P{GD11230}v21797 v21798 w1118; P{GD11230}v21798
Genomic Clones ( 1 )
	BACR32A03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP15689
Other clones	IP15889 IP18053
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	SD17859
Other clones	38659 76948 CK01.23D.G9 CK01.87A.E10 CK01.95C.E11 CK01.97A.H3 EC054799 EC055829 EK021625 EK068551 EK074220 EK075223 EK210153 EP11921 EP21408 IP18054 IP18154 IP18253 IP18254 IP18353 IP18354 SD08665
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038295 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41825
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Gyc88E CG4154 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of Gyc88E CG4154 was sequence comparison (date:031005). (FlyBase, 1996-)
Other Comments
	Gyc-89Db shows no guanylyl cyclase activity when expressed in COS-7 cells in the absence of additional subunits, in the presence of either Mg2+ or Mn2+ cofactor. However, when Gyc-89Db and Gyc88E are co-expressed in COS-7 cells, the level of guanylyl cyclase activity, in the presence of either Mg2+ or Mn2+, is higher than when Gyc88E is expressed alone. (Langlais et al., 2004) COS-7 cells co-expressing Gyc88E and Gyc-89Da or Gyc88E and Gyc-89Db accumulate low levels of cGMP under normal atmospheric oxygen conditions and are potently activated under anoxic conditions, suggesting that the Gyc88E/Gyc-89Da and Gyc88E/Gyc-89Db protein heterodimers may act as oxygen sensors. (Morton, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142167 NM_169616 NM_001043246 RefSeq (Proteins) • NP_650424 NP_731974 NP_001036711 DDBJ / EMBL / GenBank • AAF55135 AAF55135.2 AAF55135.3 AAN13628 AAN13628.1 AAN13628.2 AAQ84038 AAQ84039 ABI31167 AY376404 AY376405 Entrez Gene - A searchable database of RefSeq genes. • 41825 UniProtKB/TrEMBL • Q0KI72 Q8INF0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Adenylyl cyclase class-3/4/guanylyl cyclase, conserved site (IPR018297) Adenylyl cyclase class-3/4/guanylyl cyclase (IPR001054) Heme-NO binding (IPR011644) NO signalling/Golgi transport ligand-binding domain (IPR024096) Haem NO binding associated (IPR011645)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0038295 DRSC - Results from RNAi screens. • FBgn0038295 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038295 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4154-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038295 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41825 InParanoid A subset of ortholog calls from InParanoid. • AAEL013328-PA Aedes aegypti (mosquito) AGAP001985-PA Anopheles gambiae (mosquito) NP_001012645.1 Apis mellifera (honey bee) XP_001945138.1 Acyrthosiphon pisum (pea aphid) 268816 Branchiostoma floridae (lancelet) BGIBMGA010713-PA Bombyx mori (silk worm) CN24182 Caenorhabditis brenneri (small nematode) CBP31875 Caenorhabditis briggsae (nematode) CE36319 Caenorhabditis elegans (worm) ENSCINP00000023677 Ciona intestinalis (sea squirt) JA02029 Caenorhabditis japonica (nematode) CPIJ006958 Culex pipens (mosquito) RP30638 Caenorhabditis remanei (nematode) ENSCSAVP00000009053 Ciona savignyi (sedentary tunicate) 91580 Capitella spl (polychaete worm) 308690 Daphnia pulex (daphnia) ENSGACP00000023822 Gymnopilus aculeatus (mushroom) 104058 Lottia gigantea (owl limpet) 33782 Monocosta brevicollis (choanoflagellate) 37136 Monocosta brevicollis (choanoflagellate) 98823 Nematostella vectensis (starlet anemone) XP_001605361.1 Nasonia vitripennis (wasp) PHUM001768-PA Pediculus humanus (human body louse) PP02100 Pristionchus pacificus (nematode) Smp_149980 Schistosoma mansoni (trematode parasite) XP_001197014.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001197293.1 Strongylocentrotus purpuratus (purple sea urchin) XP_971396.2 Tribolium castaneum (red flour beetle) ENSTNIP00000008913 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000010396 Takifugu rubripes (pufferfish Takifugu) modMine - Data generated by the modENCODE project. • FBgn0038295 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15627 LH16225 ISCW017630 FBgn0241402 FBgn0215309 FBgn0211112 FBgn0190499 FBgn0179064 FBgn0248756 FBgn0159730 FBgn0146655 FBgn0124855 FBgn0109119 FBgn0094853 FBgn0090877 CPIJ006958 BGIBMGA010713 ACYPI009560 GB18735 AGAP001985 AAEL013328 PB25604 PHUM008080 GLEAN_08423
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG4154 (Langlais, 2002.7.31, Langlais, 2002.7.30, Keleman et al., 2009.8.5, Perkins et al., 2009) GYC (Morrison et al., 2000) Gyc-88E (Morton, 2004, Langlais et al., 2004, Langlais, 2003.8.28, Morton and Vermehren, 2007, Huang et al., 2007, Derbyshire et al., 2010, Vermehren-Schmaedick et al., 2010, Vermehren-Schmaedick et al., 2011) Gyc88E
Name Synonym	Guanylyl cyclase at 88E (Langlais et al., 2004)
Secondary FlyBase IDs
References ( 23 )
Generate a list of	All references relating to this gene ( 23 )
List References by type	Research paper  ( 6 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 3 )
	Vermehren-Schmaedick et al., 2011, J. Comp. Physiol. A, Neuroethol. Sens. Neural. Behav. Physiol. 197(7): 717--727 Drosophila gustatory preference behaviors require the atypical soluble guanylyl cyclases. [FBrf0214366] Derbyshire et al., 2010, J. Biol. Chem. 285(23): 17471--17478 Incorporation of tyrosine and glutamine residues into the soluble guanylate cyclase heme distal pocket alters NO and O2 binding. [FBrf0210885] Vermehren-Schmaedick et al., 2010, Genetics 186(1): 183--196 Behavioral Responses to Hypoxia in Drosophila Larvae Are Mediated by Atypical Soluble Guanylyl Cyclases. [FBrf0211824] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...