FB2025_02 , released April 17, 2025
Gene: Dmel\Cyfip
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General Information
Symbol
Dmel\Cyfip
Species
D. melanogaster
Name
Cytoplasmic FMR1 interacting protein
Annotation Symbol
CG4931
Feature Type
protein_coding_gene
FlyBase ID
FBgn0038320
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Cytoplasmic FMR1 interacting protein (Cyfip) encodes an essential protein that is a component of the WAVE actin nucleator complex. It controls actin cytoskeleton remodeling and interacts with the products of Fmr1 and Rac1. It controls morphogenesis and synapse organization. [Date last reviewed: 2020-07-09] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Sra-1, Sra1, dSra, PIR121, DSra-1

Key Links
Genomic Location
Cytogenetic map
88F1-88F1
Sequence location
Recombination map
3-57
RefSeq locus
NT_033777 REGION:15333266..15338365
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (21 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
(Chia et al., 2014)
inferred from physical interaction with UniProtKB:P40792
(Schenck et al., 2003)
inferred from physical interaction with UniProtKB:Q9NFU0
(Schenck et al., 2003)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables RNA 7-methylguanosine cap binding
inferred from biological aspect of ancestor with PANTHER:PTN000257565
(GO Reference Genome Project, 2011-)
enables small GTPase binding
inferred from electronic annotation with InterPro:IPR008081, InterPro:IPR009828
(InterPro Project Members, 2004-)
Biological Process (13 terms)
Terms Based on Experimental Evidence (10 terms)
CV Term
Evidence
References
involved_in axon guidance
inferred from mutant phenotype
(Schenck et al., 2003)
involved_in cell adhesion mediated by integrin
inferred from mutant phenotype
(Gohl et al., 2010)
involved_in cell projection assembly
inferred from mutant phenotype
(Kunda et al., 2003)
involved_in centripetally migrating follicle cell migration
inferred from mutant phenotype
(Squarr et al., 2016)
involved_in chaeta development
inferred from mutant phenotype
(Bogdan et al., 2004)
involved_in compound eye morphogenesis
inferred from mutant phenotype
(Galy et al., 2011)
involved_in cortical actin cytoskeleton organization
inferred from mutant phenotype
(Kunda et al., 2003)
involved_in positive regulation of filopodium assembly
inferred from mutant phenotype
(Squarr et al., 2016)
involved_in regulation of cell shape
inferred from mutant phenotype
(Kunda et al., 2003)
involved_in regulation of synapse organization
inferred from mutant phenotype
(Schenck et al., 2003)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in axon guidance
inferred from biological aspect of ancestor with PANTHER:PTN000257567
(GO Reference Genome Project, 2011-)
involved_in cell morphogenesis
inferred from biological aspect of ancestor with PANTHER:PTN000257565
(GO Reference Genome Project, 2011-)
involved_in cell projection assembly
inferred from biological aspect of ancestor with PANTHER:PTN000257567
(GO Reference Genome Project, 2011-)
involved_in regulation of actin filament polymerization
inferred from electronic annotation with InterPro:IPR008081
(InterPro Project Members, 2004-)
involved_in regulation of translation
inferred from biological aspect of ancestor with PANTHER:PTN000257565
(GO Reference Genome Project, 2011-)
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Galy et al., 2011)
located_in cytoplasm
inferred from direct assay
(Galy et al., 2011)
part_of SCAR complex
inferred from direct assay
(Chen et al., 2014)
inferred from direct assay
(Schenck et al., 2004)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from sequence or structural similarity with UniProtKB:Q7L576
(Schenck et al., 2001)
inferred from sequence or structural similarity with UniProtKB:Q96F07
(Schenck et al., 2001)
is_active_in neuron projection
inferred from biological aspect of ancestor with PANTHER:PTN000257567
(GO Reference Genome Project, 2011-)
part_of SCAR complex
inferred from biological aspect of ancestor with PANTHER:PTN000257565
(GO Reference Genome Project, 2011-)
is_active_in synapse
inferred from biological aspect of ancestor with PANTHER:PTN000257567
(GO Reference Genome Project, 2011-)
located_in synapse
inferred from sequence or structural similarity with UniProtKB:Q7L576
(Schenck et al., 2001)
inferred from sequence or structural similarity with UniProtKB:Q96F07
(Schenck et al., 2001)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the CYFIP family. (Q9VF87)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Cytoplasmic FMR1 interacting protein (Cyfip) encodes an essential protein that is a component of the WAVE actin nucleator complex. It controls actin cytoskeleton remodeling and interacts with the products of Fmr1 and Rac1. It controls morphogenesis and synapse organization. [Date last reviewed: 2020-07-09]
Gene Group (FlyBase)
WAVE REGULATORY COMPLEX -
The WAVE Regulatory Complex (WRC) is a multi-subunit complex that controls actin cytoskeletal dynamics. WRC activates the Arp2/3-mediated actin nucleation. WRC contains the nucleation-promoting factor SCAR, a member of the WASP family. (Adapted from FBrf0208042 and FBrf0224564).
Protein Function (UniProtKB)
Plays a role in guidance and morphology of central and peripheral axons and in synaptic morphology. Also required for formation of cell membrane protrusions and for bristle development (PubMed:12818175, PubMed:14588242, PubMed:15269173, PubMed:15385157). Plays a role in regulating mitochondrial activity, energy metabolism and membrane potential which maintains normal gamma-aminobutyric acid (GABA) signaling and ensures normal social behavior (PubMed:32200800).
(UniProt, Q9VF87)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Cyfip for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VF87)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Cyfip-RA
FBtr0083085
NM_142190
4628
1291
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Cyfip-PA
FBpp0082541
149.3
1291
7.13
Q9VF87
NP_650447
AAF55173
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts with Fmr1 and Rac1. Component of the WAVE complex composed of Hem/Kette, Scar/Wave and Cyfip where it binds through its C-terminus directly to Hem.

(UniProt, Q9VF87)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyfip using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.58

Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
indirect flight muscle cell
(Katti et al., 2017)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
presumptive embryonic/larval central nervous system
(Schenck et al., 2004)
embryonic stage 5
organism | ubiquitous
(Schenck et al., 2003)
embryonic stage 12
embryonic/larval gut
(Schenck et al., 2003)
embryonic stage 12 -- 17
larval ventral nerve cord
(Schenck et al., 2003)
embryonic stage 17
larval brain
(Schenck et al., 2003)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Schenck et al., 2003)
adult stage | female
ovary
(Schenck et al., 2003)
Additional Descriptive Data

Cyfip transcripts are detected in early embryos by in situ hybridization. Starting at stage 12, they are detected in the gut and CNS and persist longer in the brain and ventral nerve cord.

(Schenck et al., 2003)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 17
larval central nervous system
(Schenck et al., 2003)
embryonic stage 13 -- 16
embryonic/larval gut
(Schenck et al., 2003)
embryonic stage 13 -- 17
larval ventral nerve cord
(Schenck et al., 2003)
ventral midline of embryo
(Schenck et al., 2003)
larval longitudinal connective
(Schenck et al., 2003)
symmetrical commissure
(Schenck et al., 2003)
embryonic stage 14 -- 16
symmetrical commissure
(Schenck et al., 2004)
embryonic stage 14 -- 17
larval longitudinal connective
(Schenck et al., 2004)
embryonic stage 17
embryonic/larval neuromuscular junction
(Schenck et al., 2003)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 11 -- 17
organism
(Schenck et al., 2003)
embryonic stage 12 -- 17
(Schenck et al., 2004)
cell

Comment: expression assayed in cultured S2 cells

(Schenck et al., 2004)
Additional Descriptive Data

Cyfip protein is detected at very low levels in early embryos and then at higher levels at later embryonic stages on western blots. It is observed in the gut and CNS but persists longer in the CNS. It accumulates in CNS axons along commissures and longitudinal connectives. It is present in neuromuscular junctions in stage 17 embryos.

(Schenck et al., 2003)
Marker for
 
larval longitudinal connective
(Schenck et al., 2004)
Subcellular Localization
CV Term
Evidence
References
located_in apical part of cell
inferred from direct assay
(Galy et al., 2011)
located_in cytoplasm
inferred from direct assay
(Galy et al., 2011)
part_of SCAR complex
inferred from direct assay
(Chen et al., 2014)
inferred from direct assay
(Schenck et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cyfip in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Cyfip
Transgenic constructs containing regulatory region of Cyfip
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3R)Exel6174
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal | embryonic stage | germline clone
lethal | pupal stage | recessive
lethal | recessive
lethal - all die before end of P-stage (with Cyfip85.1)
lethal - all die before end of P-stage (with CyfipEP3267)
partially lethal - majority live
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4elav-C155
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
viable (with CyfipI471V)
viable (with CyfipP760L)
Sterility
Allele
fertile
fertile (with CyfipI471V)
fertile (with CyfipP760L)
Other Phenotypes
Allele
abnormal behavior | adult stage (with CyfipI471V)
abnormal behavior | adult stage (with CyfipP760L)
abnormal behavior | adult stage | dominant
abnormal cell migration | oogenesis, with Scer\GAL4GR1
abnormal flight, with Scer\GAL4Mef2.PR
abnormal neuroanatomy, with Scer\GAL4ey.PH
abnormal neuroanatomy | adult stage (with CyfipI471V)
abnormal neuroanatomy | adult stage (with CyfipP760L)
abnormal neuroanatomy | adult stage | dominant
abnormal neuroanatomy | dominant
abnormal neuroanatomy | dominant | larval stage
decreased rate of adult locomotory behavior (with CyfipI471V)
decreased rate of adult locomotory behavior (with CyfipP760L)
decreased rate of movement | adult stage (with CyfipI471V)
decreased rate of movement | adult stage (with CyfipP760L)
some die during pharate adult stage (with Cyfip85.1)
some die during pharate adult stage (with CyfipEP3267)
visible | adult stage, with Cyfip85.1, Scer\GAL4lz-gal4
visible | adult stage, with CyfipRNAi.UAS.cGa, Scer\GAL4lz-gal4
visible | adult stage, with Scer\GAL4lz-gal4
visible | adult stage | somatic clone
visible | pharate adult stage (with Cyfip85.1)
visible | pharate adult stage (with CyfipEP3267)
Phenotype manifest in
Allele
actin-based cell projection | oogenesis, with Scer\GAL4GR1
adult head & macrochaeta, with Scer\GAL4sca.PC
adult s-LNv neuron | adult stage (with CyfipI471V)
adult s-LNv neuron | adult stage (with CyfipP760L)
axon
axon | adult stage (with CyfipI471V)
axon | adult stage (with CyfipP760L)
bouton
centripetal follicle cell, with Scer\GAL4GR1
cone cell | pharate adult stage (with Cyfip85.1)
cone cell | pharate adult stage (with CyfipEP3267)
cone cell | pupal stage (with Cyfip85.1)
cone cell | pupal stage (with CyfipEP3267)
egg, with Scer\GAL4GR1
egg | maternal effect, with Scer\GAL4GR1
egg | maternal effect, with Scer\GAL4tj.PU
egg chamber, with Scer\GAL4GR1
ellipsoid body | adult stage
ellipsoid body | adult stage (with CyfipI471V)
ellipsoid body | adult stage (with CyfipP760L)
embryonic/larval neuromuscular junction
eye, with Scer\GAL4ey.PH
eye | adult stage, with Cyfip85.1, Scer\GAL4lz-gal4
eye | adult stage, with CyfipRNAi.UAS.cGa, Scer\GAL4lz-gal4
eye | adult stage, with Scer\GAL4lz-gal4
eye | adult stage | somatic clone
eye | pharate adult stage (with Cyfip85.1)
eye | pharate adult stage (with CyfipEP3267)
filopodium | oogenesis, with Scer\GAL4GR1
interommatidial bristle | pharate adult stage (with Cyfip85.1)
interommatidial bristle | pharate adult stage (with CyfipEP3267)
interommatidial bristle | pharate adult stage | somatic clone
larval intersegmental nerve
larval longitudinal connective
larval neuromuscular junction & bouton, with Scer\GAL4elav-C155
lens | pharate adult stage (with Cyfip85.1)
lens | pharate adult stage (with CyfipEP3267)
lens | pharate adult stage | somatic clone
lobe system of adult mushroom body
lobe system of adult mushroom body (with CyfipI471V)
lobe system of adult mushroom body (with CyfipP760L)
microchaeta, with Scer\GAL4sca.PC
neuromuscular junction & synapse
ommatidium, with Scer\GAL4ey.PH
ommatidium | pharate adult stage (with Cyfip85.1)
ommatidium | pharate adult stage (with CyfipEP3267)
ommatidium | pharate adult stage | somatic clone
photoreceptor | adult stage, with Cyfip85.1, Scer\GAL4lz-gal4
photoreceptor | adult stage, with CyfipRNAi.UAS.cGa, Scer\GAL4lz-gal4
photoreceptor | adult stage, with Scer\GAL4lz-gal4
photoreceptor | adult stage | somatic clone
photoreceptor | pupal stage (with Cyfip85.1)
photoreceptor | pupal stage (with CyfipEP3267)
photoreceptor cell & growth cone & larval brain, with Scer\GAL4ey.PH
photoreceptor neuron | wandering third instar larval stage, with Scer\GAL4ey.PH
presumptive embryonic/larval central nervous system | germline clone
retina | pharate adult stage (with Cyfip85.1)
retina | pharate adult stage (with CyfipEP3267)
retina | pharate adult stage | somatic clone
rhabdomere | adult stage | somatic clone
rhabdomere | pharate adult stage (with Cyfip85.1)
rhabdomere | pharate adult stage (with CyfipEP3267)
rhabdomere | pupal stage (with Cyfip85.1)
rhabdomere | pupal stage (with CyfipEP3267)
symmetrical commissure
synapse
trichogen cell & actin filament & pupa, with Scer\GAL4sca.PC
vertical lobe of adult mushroom body
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\CYFIP1
13 of 14
Yes
Yes
1  
Hsap\CYFIP2
13 of 14
Yes
Yes
Hsap\CYRIA
1 of 14
No
No
Hsap\CYRIB
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Cyfip1
13 of 14
Yes
Yes
Rnor\Cyfip2
11 of 14
No
Yes
Rnor\Cyria
1 of 14
No
No
Rnor\Cyrib
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Cyfip2
14 of 14
Yes
Yes
Mmus\Cyfip1
12 of 14
No
Yes
Mmus\Cyria
1 of 14
No
No
Mmus\Cyrib
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\cyfip2
8 of 13
Yes
Yes
Xtro\cyfip1
7 of 13
No
Yes
Xtro\tmem151b
1 of 13
No
Yes
Danio rerio (Zebrafish) (6)
Drer\cyfip2
13 of 14
Yes
Yes
Drer\cyfip1
12 of 14
No
Yes
Drer\fam49a
1 of 14
No
No
Drer\fam49al
1 of 14
No
No
Drer\fam49ba
1 of 14
No
No
Drer\fam49bb
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\gex-2
13 of 14
Yes
Yes
Cele\R07G3.8
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP000195
12 of 12
Yes
Yes
Agam\AgaP_AGAP006091
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\PIR121
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Cyfip. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
cyri
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
CYFIP1; cytoplasmic FMR1 interacting protein 1
13 of 14
606322
       
      CYFIP2; cytoplasmic FMR1 interacting protein 2
      13 of 14
      606323
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Cyfip - Abi
      anti bait coimmunoprecipitation, peptide massfingerprinting
      (Gautier et al., 2011)
      Cyfip - Abl
      pull down, Identification by mass spectrometry
      (Cheong et al., 2020)
      Cyfip - Arf1
      pull down, western blot
      (Humphreys et al., 2012)
      Cyfip - Chc
      anti bait coimmunoprecipitation, peptide massfingerprinting
      (Gautier et al., 2011)
      Cyfip - Fmr1
      anti bait coimmunoprecipitation, western blot, pull down, autoradiography
      (Bozzetti et al., 2015, Schenck et al., 2003)
      Cyfip - HSPC300
      pull down, molecular sieving, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot
      (Chaudhari et al., 2024, Chen et al., 2014, Chia et al., 2014, Gautier et al., 2011, Ismail et al., 2009, Qurashi et al., 2007)
      Cyfip - Hem
      anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based, two hybrid, anti bait coimmunoprecipitation, peptide massfingerprinting
      (Gautier et al., 2011, Guruharsha et al., 2011, Bogdan et al., 2004, Schenck et al., 2004)
      Cyfip - Rac1
      anti bait coimmunoprecipitation, western blot
      (Schenck et al., 2003)
      Cyfip - SCAR
      anti bait coimmunoprecipitation, peptide massfingerprinting, anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based
      (Gautier et al., 2011, Guruharsha et al., 2011, Schenck et al., 2004)
      Cyfip - WASp
      pull down, western blot
      (Bogdan et al., 2005)
      Cyfip - drk
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Friedman et al., 2011)
      Summary of Genetic Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Cyfip
      enhanceable
      Hem
      (Bogdan et al., 2004)
      Cyfip
      suppressible
      Hem
      (Bogdan et al., 2004)
      Cyfip
      suppressible
      WASp
      (Bogdan et al., 2004)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Fmr1
      enhanceable
      Cyfip
      (Schenck et al., 2003)
      Fmr1
      suppressible
      Cyfip
      (Schenck et al., 2004, Schenck et al., 2003)
      fra
      enhanceable
      Cyfip
      (Chaudhari et al., 2024)
      Hem
      enhanceable
      Cyfip
      (Bogdan et al., 2004)
      Rac1
      enhanceable
      Cyfip
      (Schenck et al., 2003)
      Rac1
      suppressible
      Cyfip
      (Banka et al., 2022, Schenck et al., 2003)
      External Data
      Subunit Structure (UniProtKB)
      Interacts with Fmr1 and Rac1. Component of the WAVE complex composed of Hem/Kette, Scar/Wave and Cyfip where it binds through its C-terminus directly to Hem.
      (UniProt, Q9VF87 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-57
      Cytogenetic map
      88F1-88F1
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      88F1-88F1
      Limits computationally determined from genome sequence between P{lacW}MRG15j6A3 and P{PZ}l(3)0653606536
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      88E13-88F1
      (Langmann, 2001.4.3)
      Experimentally Determined Recombination Data
      Location

      3-57

      (FlyBase, 2016)

      3-56.1

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (7)
      Genomic Clones (12)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (112)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Gautier et al., 2011, Schenck et al., 2003)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments

          S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

          (Stroschein-Stevenson et al., 2006)

          dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

          (Rogers et al., 2003)

          Sra-1 controls neural connectivity in the nervous system.

          (Schenck et al., 2003)
          Relationship to Other Genes
          Source for database merge of
          Additional comments

          Source for identity of Sra-1 CG4931 was sequence comparison ( date:010419 ).

          (FlyBase, 1996-)
          Nomenclature History
          Source for database identify of

          Source for identity of: Sra-1 CG4931

          (FlyBase, 1996-)

          Source for identity of: Cyfip Sra-1

          (Schenck et al., 2003)
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (24)
          Reported As
          Symbol Synonym
          CG4931
          (Schwartz et al., 2016, Rosa-Ferreira et al., 2015, Schertel et al., 2013, Christis and Munro, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Rogers et al., 2003)
          CYFIP
          (Bozzetti et al., 2015, Wang et al., 2012, Galy et al., 2011, Reeve et al., 2008, Qurashi et al., 2007, Qurashi et al., 2007, Govek et al., 2005, Schenck et al., 2004, Billuart and Chelly, 2003, Schenck et al., 2003, Schenck et al., 2003, Schenck, 2001.1.9, Schenck et al., 2001)
          CYFIP1
          (Ueoka et al., 2019)
          CYFIP1/2
          (Zhang and Broadie, 2005)
          Cyfip
          (Mariano et al., 2024, Mariano et al., 2023, Banka et al., 2022, Hirschhäuser et al., 2021, Kanellopoulos et al., 2020)
          DSra-1
          (Langmann et al., 2002, Langmann, 2001.4.3)
          PIR121
          (Law et al., 2013, Qurashi et al., 2007)
          PIR121/Sra-1
          (Vartiainen and Machesky, 2004)
          Sra
          (Bogdan et al., 2004)
          Sra-1
          (Adashev et al., 2022, Okuda et al., 2022, Cheong et al., 2020, Mariano et al., 2020, Takai et al., 2020, Yusuff et al., 2020, Gao et al., 2019, Katti et al., 2017, Bolin et al., 2016, Squarr et al., 2016, Aradska et al., 2015, Eikenes et al., 2015, Van Bortle et al., 2015, Toret et al., 2014, Kwon et al., 2013, Schertel et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Rohn et al., 2011, Stephan et al., 2011, Zhu and Bhat, 2011, Fricke et al., 2009, Czech et al., 2008, Stroschein-Stevenson et al., 2006, Yepiskoposyan et al., 2006, Bogdan et al., 2005, Rogers et al., 2003)
          Sra-1/CYFIP
          (Gohl et al., 2010)
          Sra1
          (Williams and Horne-Badovinac, 2024, Williams et al., 2022, Gao et al., 2019, Humphreys et al., 2012, Peltan et al., 2012, D'Ambrosio and Vale, 2010, Kunda et al., 2003)
          Sra1/PIR121
          (Chen et al., 2007)
          cyfip
          (Chaudhari et al., 2024, Chaudhari et al., 2021, Zhao et al., 2013)
          dCYFIP
          (Abekhoukh et al., 2017)
          dCYFIP1
          (Drozd et al., 2018)
          dCyfip
          (Woo et al., 2019)
          dSra
          (Chen et al., 2014, Chia et al., 2014, Ismail et al., 2009)
          sra-1
          (Chen et al., 2014, Gohl et al., 2010)
          sra1
          (Bischoff et al., 2021, Hamp et al., 2016, Bogdan et al., 2004)
          Name Synonyms
          Cytoplasmic FMR1 interacting protein
          Specifically Rac1-associated protein 1
          (Chang et al., 2008)
          specifically Rac1-associated protein 1
          (Bogdan et al., 2004)
          specifically, Rac-1 associated protein 1
          (Stephan et al., 2011)
          Secondary FlyBase IDs
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 49 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (117)