• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\CG6194

General Information
Symbol	Dmel\CG6194	Species	D. melanogaster
Name		Annotation symbol	CG6194
Feature type	protein_coding_gene	FlyBase ID	FBgn0038325
Gene Model Status	Current	Stock availability	6 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88F1-88F1	Sequence location	3R:11,179,442..11,182,646 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\CG6194 (FBgn0038325). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: cysteine-type endopeptidase activity. There is experimental evidence that it is involved in the biological process: autophagic cell death; salivary gland cell autophagic cell death. 5 alleles are reported. The phenotype of these alleles is annotated with: trichogen cell. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase C54. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1627764_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG6194. Gene sequence location is 3R:11179442..11182646.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88F1-88F1   Limits computationally determined from genome sequence between P{lacW}MRG15j6A3 and P{PZ}l(3)0653606536
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CG6194 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG6194-RA FBtr0083115  NM_142195   2665   668
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CG6194-PA   FBpp0082569   74.8   668   5.86     NP_650452   AAF55180
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C54 (IPR005078)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA539394   AE014297 AAF55180   AW942448   AY069453 AAL39598   BG641430   BI163505   BI634843   BP546589   BT100116   CA804286   CA806505   CK660956   CO329857   EC204867   EL879996   EL879997   EV583544   GH750944   GH751745   GH756767   GH757936   GH760855   GH776689   GH778997   GH788747   GH792365   GH794155   GH796883   GH798366   GH801741   GH802099   GH804553   GH807283   GH810523   GH812423   GH816029   GH822821   GH823983   GH824072   GH825035   GH826396   GH826997   GH827532   GH829841   GH838951   GH846607   GH849066   GH849104   GH852496   GH852620   GH857117   GH862737   GH865985   GH868477   GH868936   GH869177   GH869517   GH870069   GH871129   GH872871   GH873688   GH874910   GH880134   GH882625   GH882639   GH883003   GH884195   GH884347   GH888832   GH891124   GH893865   GH897926   GH898350   GH899557   GH899823   GH900970   GH903218   GH908171   GH909068   GH909552   GH910530   GH914247   GH914722   GH915630   GH922749   GH924902   GH925546   GH926348   GH932420   GH934409   GH936617
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VF80 Q8T0A2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) Comment:rapidly degraded organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   central brain primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference pupal stage   salivary gland   (Lee et al., 2003)
Additional Descriptive Data
	The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue. (Lee et al., 2003)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    122.9 Larval Midgut    134.9 Larval Hindgut    268.8 Larval Malpighian Tubules    139.1 Larval Fat Body    61 Larval Salivary Gland    478.3 Larval Trachea    102.55 Larval Carcass    84.375 Adult Head    100.3 Adult Eye    130.65 Adult Brain    174.3 Adult Thoracic-Abdominal Ganglion    149.6 Adult Crop    93.5 Adult Midgut    110.7 Adult Hindgut    232.9 Adult Malpighian Tubules    234 Adult Fat Body    73.6 Adult Salivary Gland    297.3 Adult Heart    94.65 Adult VirginFemale Spermatheca    105.7 Adult InseminatedFemale Spermatheca    141 Adult Ovary    275.6 Adult Testis    29.7 Adult Male Accessory Gland    159.3 Adult Carcass    107.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    100.3   NA  Eye    130.65   NA  Brain    174.3   122.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    149.6   NA  Crop    93.5   134.9  Midgut    110.7   268.8  Hindgut    232.9   139.1  Malpighian Tubules    234   61  Fat Body    73.6   478.3  Salivary Gland    297.3   NA  Heart    94.65   102.55  Trachea    NA   NA  VirginFemale Spermatheca    105.7   NA  InseminatedFemale Spermatheca    141   NA  Ovary    275.6   NA  Testis    29.7   NA  Male Accessory Gland    159.3   84.375  Carcass    107.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    7 central nervous system, pupae P8    7 head, virgin 1-day female    5 head, virgin 4-day female    5 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    7 head, mated 20-day male    9 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    14 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    1 fat body, white prepupae    4 fat body, pupae P8    4 carcass, larvae L3 wandering    5 carcass, 1-day adult    4 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    17 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr  (43) white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (39) embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr  (43) white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    39 embryo 02-04hr    13 embryo 04-06hr    17 embryo 06-08hr    17 embryo 08-10hr    19 embryo 10-12hr    18 embryo 12-14hr    12 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    12 embryo 20-22hr    15 embryo 22-24hr    17 larva L1    10 larva L2    7 larva L3 12hr old    9 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    43 white prepupae 24hr    17 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    12 adult male 01day    10 adult male 05day    9 adult male 30day    10 adult female 01day    17 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    14 Schneider line 2 Sg4    12 embryonic 1182-4H    12 embryonic GM2    21 embryonic Kc167    21 embryonic S1    11 embryonic S3    5 leg disc CME L1    6 wing disc CME-W2    7 wing disc ML-DmD8    7 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    9 wing disc ML-DmD21    10 wing disc ML-DmD32    9 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    8 antennal disc ML-DmD20-c5    11 mixed discs ML-DmD4-c1    7 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    7 ovary fGS/OSS    7 ovary OSC    7 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    6 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    14 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    11 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    9 Paraquat 10 mM 48 hrs, 4-day adult    11 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_27 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD17482 RE44406 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038325 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6194-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG6194f01043 CG6194P0997 viable, with Scer\GAL4pnr-MD237 CG6194GD11855 Sterility Allele fertile CG6194f01043 CG6194P0997 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 CG6194GD11855 Phenotype manifest in Allele chaeta | supernumerary, with Scer\GAL4pnr-MD237 CG6194GD11855 trichogen cell, with Scer\GAL4pnr-MD237 CG6194GD11855
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of CG6194 Class Mutagen Stocks Known lesion CG6194f01043 piggyBac transposase 2 -- CG6194P0997 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG6194 Class Mutagen Stocks Known lesion CG6194GD11855 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes CG6194KK102037 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CG6194NIG.6194R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6174 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11855} NA P{KK102037} NA P{NIG.6194R} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}CG6194f01043 NA insertion of enhancer trap P{lacW}CG6194P0997 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
autophagic cell death inferred from expression pattern (Gorski et al., 2003) salivary gland cell autophagic cell death inferred from expression pattern (Gorski et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
cysteine-type endopeptidase activity inferred from sequence or structural similarity with NCBI:NP_116241 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces ATG4 (Thumm and Kadowaki, 2001)
Biological Process
CV term Evidence References
autophagy inferred from sequence or structural similarity with NCBI:NP_116241 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces ATG4 (Thumm and Kadowaki, 2001) proteolysis inferred from sequence or structural similarity with NCBI:NP_116241 (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG6194 allele Gene References CG6194P0997 ct (Thumm and Kadowaki, 2001) Dl (Thumm and Kadowaki, 2001) dx (Thumm and Kadowaki, 2001) H (Thumm and Kadowaki, 2001) N (Thumm and Kadowaki, 2001) Ser (Thumm and Kadowaki, 2001) sno (Thumm and Kadowaki, 2001) vg (Thumm and Kadowaki, 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66931 DroID - A comprehensive database of gene and protein interactions. • FBgn0038325 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41868
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6CNR9N Dipteran inclusive ortholog search EOG6001SF Insect inclusive ortholog search EOG6K0PHR Arthropod inclusive ortholog search EOG6D2556 Metazoa inclusive ortholog search EOG6XM71X
Orthologs in Drosophila Species (EOG6CNR9N)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CG6194     Drosophila simulans   Dsim\GD20357 Y   Drosophila sechellia   Dsec\GM25780 Y   Drosophila erecta   Dere\GG20629 Y   Drosophila yakuba   Dyak\GE26385 Y   Drosophila ananassae   Dana\GF23235 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19429 Y   Drosophila persimilis   Dper\GL13755 Y   Drosophila willistoni   Dwil\GK11310 Y   Drosophila virilis   Dvir\GJ10670 Y   Drosophila mojavensis   Dmoj\GI10728 Y   Drosophila grimshawi   Dgri\GH16873 Y
Orthologs in non-Drosophila Dipterans (EOG6001SF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010516   Anopheles gambiae Malaria mosquito  Agam\AGAP004023   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002380
Orthologs in non-Dipteran Insects (EOG6K0PHR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45197   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22893   Atta cephalotes Leafcutter ant  Acep\ACEP20233   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16916   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21074   Linepithema humile Argentine ant  Lhum\LH22618   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18243   Solenopsis invicta Red fire ant  Sinv\SINV17961   Bombyx mori Silkmoth  Bmor\BGIBMGA004926   Tribolium castaneum Red flour beetle  Tcas\TC014208
Orthologs in non-Insect Arthropods (EOG6D2556)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_302128   Ixodes scapularis Deer tick  Isca\ISCW010488
Orthologs in non-Arthropod Metazoa (EOG6XM71X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\atg-4.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027939gn   Danio rerio Zebrafish  Drer\BX547996.2   Danio rerio Zebrafish  Drer\atg4c   Xenopus tropicalis Western clawed frog  Xtro\atg4c   Xenopus tropicalis Western clawed frog  Xtro\atg4d   Gallus gallus Domestic chicken  Ggal\ATG4C   Mus musculus House mouse  Mmus\Atg4c   Mus musculus House mouse  Mmus\Atg4d   Rattus norvegicus Norway rat  Rnor\Atg4c   Homo sapiens Human  Hsap\ATG4D   Homo sapiens Human  Hsap\ATG4C
Human Orthologs (2)
Gene OMIM HGNC Hsap\ATG4D     Hsap\ATG4C
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20357 Drosophila sechellia Dsec\GM25780 Drosophila erecta Dere\GG20629 Drosophila yakuba Dyak\GE26385 Drosophila ananassae Dana\GF23235 Drosophila pseudoobscura pseudoobscura Dpse\GA19429 Drosophila persimilis Dper\GL13755 Drosophila willistoni Dwil\GK11310 Drosophila virilis Dvir\GJ10670 Drosophila mojavensis Dmoj\GI10728 Drosophila grimshawi Dgri\GH16873
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	18403 w1118; PBac{WH}CG6194f01043 36340 y1 w*; P{lacW}CG6194P0997
Harvard	- PBac{WH}CG6194f01043
VDRC	v108299 P{KK102037}VIE-260B v22293 w1118; P{GD11855}v22293 v22294 w1118; P{GD11855}v22294
Genomic Clones ( 2 )
	BACR13A02 BACR17K19 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 87 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD17482 RE44406
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP19954 RE02923 RH39086 SD12768
Other clones	191107 Dmel_dig_006827_0610_2500 EK294036 ESG013c.E21_H05 ESG0112d.B21_G09 FGH108A05 IP09860 RP003010263 RP003017560 RP003018529 RP003023389 RP003034801 RP003035453 RP003065211 RP003091566 RP003091900 RP003106692 RP003117657 RP003119457 RP003136920 RP003148447 RP003163001 RP003164181 RP003164579 RP003168036 RP003194136 RP003208393 RP003210230 RP003219914 RP003229888 RP003238406 RP003241940 RP003248598 RP003252712 RP003254228 RP003255939 RP003256949 RP003259482 RP003261204 RP003274771 RP003274993 RP003275172 RP003287328 RP003292547 RP003294508 RP003309320 RP003313971 RP003315238 RP003322191 RP003323948 RP003331162 RP003347497 RP003357064 RP003362829 RP003368148 RP003394243 RP003405713 RP003444839 RP003446651 RP003453439 RP003456965 RP003457179 RP003483197 RP003484936 RP003486961 RP003494735 RP003507082 RP003511177 RP003541541 RP003545699 RP003546691 RP003582900 RP003584946 RP003598757 RP003625568 RP003635225 RP003649396 RP003651195 RP003658100 RP003663036 RP003667391
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038325 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41868
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142195 RefSeq (Proteins) • NP_650452 DDBJ / EMBL / GenBank • AA539394 AAF55180 AAL39598 AW942448 AY069453 Entrez Gene - A searchable database of RefSeq genes. • 41868 UniProtKB/TrEMBL • Q8T0A2 Q9VF80
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase C54 (IPR005078) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD17482 RE44406
Linkouts
	BioGRID - A database of protein and genetic interactions • 66931 DroID - A comprehensive database of gene and protein interactions. • FBgn0038325 DRSC - Results from RNAi screens. • FBgn0038325 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038325 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6194-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038325 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41868 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41868 modMine - Data generated by the modENCODE project. • FBgn0038325
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Atg4b (Érdi et al., 2012) CG6194 (Czech et al., 2008, Chittaranjan et al., 2009, Liu and Lehmann, 2008, Keleman et al., 2009.8.5, Érdi et al., 2012, Mackay et al., 2012, Japanese National Institute of Genetics, 2012.5.21) DmAtg4-like (Chittaranjan et al., 2009)
Name Synonym
Secondary FlyBase IDs
References ( 22 )
Generate a list of	All references relating to this gene ( 22 )
List References by type	Research paper  ( 8 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 5 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 )
Recent research papers ( 2 )
	Mackay et al., 2012, Nature 482(7384): 173--178 The Drosophila melanogaster Genetic Reference Panel. [FBrf0217434] Érdi et al., 2012, Autophagy 8(7): 1124--1135 Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. [FBrf0219844] Show all research papers ...