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Gene Dmel\Rbf2

General Information
Symbol	Dmel\Rbf2	Species	D. melanogaster
Name	Retinoblastoma-family protein 2	Annotation symbol	CG5083
Feature type	protein_coding_gene	FlyBase ID	FBgn0038390
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	89A8-89A8	Sequence location	3R:11,791,173..11,793,860 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Retinoblastoma-family protein 2 is referred to in FlyBase by the symbol Dmel\Rbf2 (CG5083, FBgn0038390). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription; transcription factor binding; transcription regulatory region DNA binding. There is experimental evidence that it is involved in the biological process: negative regulation of S phase of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter. 11 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Cyclin-like; Domain of unknown function DUF3452, retinoblastoma-associated; Retinoblastoma-associated protein, A-box; Retinoblastoma-associated protein, B-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1638806_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rbf2. Gene sequence location is 3R:11791173..11793860.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN25501 HFA16816 Controlled Vocabulary Terms negative regulation of S phase of mitotic cell cycle transcription factor binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89A8-89A8   Limits computationally determined from genome sequence between P{PZ}blp01618 and P{PZ}gish04895&P{EP}EP3171
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89B1-89B4     (Stevaux, 1999.10.15)
Experimentally Determined Recombination Data
Location	3- (Stevaux and Dyson, 2000)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rbf2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rbf2-RA FBtr0083213  NM_079648   2688   783
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rbf2-PA   FBpp0082667   89.8   783   8.05     NP_524372   AAF55258
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202554   AA441035   AE014297 AAF55258   AF195899 AAF66827   AF197059 AAF62399   AY071301 AAL48923   BI162298   BI164562   BI170256   BI212541   BI215298   BI236865   BI352825   CO262554   CO291367   CO303901   CO303906   CO303921   CO303923   CO303925   CO303927   CO318915   CO319729   CO322543   EC057228   EC058988   EC067235   EC078521   EC089923   EC196524   EC229100   EC230930   EC235026   EC240583   EC244545   EC246492   EC246493   EC247857   EV581334
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9NIS1 Q9VF04
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0038390    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0038390 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0038390 expression level Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3740) embryo 02-04hr  (2311) embryo 04-06hr  (2933) embryo 06-08hr  (1692) embryo 08-10hr  (941) embryo 10-12hr  (340) embryo 12-14hr  (247) embryo 14-16hr  (154) embryo 16-18hr  (110) embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2  (129) larva L3 puffstage 3-6  (181) larva L3 puffstage 7-9  (264) white prepupae new  (232) white prepupae 12hr  (243) white prepupae 24hr  (232) pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day  (584) adult female 05day  (1594) adult female 30day  (1673) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3740) embryo 02-04hr  (2311) embryo 04-06hr  (2933) embryo 06-08hr  (1692) embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day  (1594) adult female 30day  (1673) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0038390 expression level Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3740) embryo 02-04hr  (2311) embryo 04-06hr  (2933) embryo 06-08hr  (1692) embryo 08-10hr  (941) embryo 10-12hr  (340) embryo 12-14hr  (247) embryo 14-16hr  (154) embryo 16-18hr  110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2  129 larva L3 puffstage 3-6  (181) larva L3 puffstage 7-9  (264) white prepupae new  (232) white prepupae 12hr  (243) white prepupae 24hr  (232) pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day  (584) adult female 05day  (1594) adult female 30day  (1673) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3740) embryo 02-04hr  2311 embryo 04-06hr  (2933) embryo 06-08hr  1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day  1594 adult female 30day  1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3740 embryo 02-04hr    2311 embryo 04-06hr    2933 embryo 06-08hr    1692 embryo 08-10hr    941 embryo 10-12hr    340 embryo 12-14hr    247 embryo 14-16hr    154 embryo 16-18hr    110 embryo 18-20hr    52 embryo 20-22hr    51 embryo 22-24hr    79 larva L1    81 larva L2    91 larva L3 12hr old    57 larva L3 puffstage 1-2    129 larva L3 puffstage 3-6    181 larva L3 puffstage 7-9    264 white prepupae new    232 white prepupae 12hr    243 white prepupae 24hr    232 pupae 2d postWPP    79 pupae 3d postWPP    38 pupae 4d postWPP    45 adult male 01day    38 adult male 05day    45 adult male 30day    53 adult female 01day    584 adult female 05day    1594 adult female 30day    1673 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0038390    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0038390 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0038390 expression level Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0038390 expression level Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    70.75 Larval Midgut    2.6 Larval Hindgut    7 Larval Malpighian Tubules    4.3 Larval Fat Body    1.2 Larval Salivary Gland no informative data Larval Trachea    24.6 Larval Carcass no informative data Adult Head    2.8 Adult Eye    6.775 Adult Brain    3.2 Adult Thoracic-Abdominal Ganglion    5.9 Adult Crop    2.6 Adult Midgut    2.2 Adult Hindgut    3.4 Adult Malpighian Tubules    1.8 Adult Fat Body    5.8 Adult Salivary Gland    3.3 Adult Heart    3.725 Adult VirginFemale Spermatheca    1.8 Adult InseminatedFemale Spermatheca    5.7 Adult Ovary    321.3 Adult Testis    1.9 Adult Male Accessory Gland    6.7 Adult Carcass    3.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.8   NA  Eye    6.775   NA  Brain    3.2   70.75  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    5.9   NA  Crop    2.6   2.6  Midgut    2.2   7  Hindgut    3.4   4.3  Malpighian Tubules    1.8   1.2  Fat Body    5.8   no informative data  Salivary Gland    3.3   NA  Heart    3.725   24.6  Trachea    NA   NA  VirginFemale Spermatheca    1.8   NA  InseminatedFemale Spermatheca    5.7   NA  Ovary    321.3   NA  Testis    1.9   NA  Male Accessory Gland    6.7   no informative data  Carcass    3.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038390 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5083-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rbf2 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Rbf2 Class Mutagen Stocks Known lesion Rbf2G5174 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rbf2 Class Mutagen Stocks Known lesion Rbf2KK104541 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbf2dsRNA.cFa in vitro construct - RNAi 0 Yes Rbf2dsRNA.cLa in vitro construct - RNAi 0 Yes Rbf2GD4124 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Rbf2ie1.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rbf2Kr.T:Scer\GAL4 in vitro construct - coding region fusion 0 Yes Rbf2Scer\UAS.cSa in vitro construct - regulatory fusion 0 Yes Rbf2T:Zzzz\FLAG in vitro construct - regulatory fusion 0 Yes Rbf2tSa in vitro construct - genomic fragment 0 Yes Rbf2tSb in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel7327 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4124} NA P{KK104541} NA P{UAS-Rbf2.S} NA characterization construct P{(CG4287)-CG5516-Rbf2.S} NA P{CG4287-CG5516-(Rbf2).S} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Rbf2G5174 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
contributes_to RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription inferred from physical interaction with E2f2 (Stevaux et al., 2002) transcription factor binding inferred from physical interaction with Dp (Stevaux et al., 2002) inferred from physical interaction with E2f2 (Stevaux et al., 2002) transcription regulatory region DNA binding inferred from direct assay (Ullah et al., 2007)
Biological Process
CV term Evidence References
negative regulation of S phase of mitotic cell cycle inferred from direct assay (Stevaux et al., 2002) negative regulation of transcription from RNA polymerase II promoter inferred from genetic interaction with Rbf (Lewis et al., 2004)
Cellular Component
CV term Evidence References
Myb complex inferred from direct assay (Lewis et al., 2004, Korenjak et al., 2004) Rb-E2F complex inferred from direct assay (Stevaux et al., 2002)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from sequence or structural similarity with EMBL:U50850 (Stevaux and Dyson, 2000)
Biological Process
CV term Evidence References
regulation of cell cycle inferred from sequence or structural similarity with EMBL:U50850 (Stevaux and Dyson, 2000)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity (Stevaux and Dyson, 2000) Rb-E2F complex traceable author statement (Lee and Orr-Weaver, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rbf2 allele Gene References Rbf2Scer\UAS.cSa E2f2 (Stevaux et al., 2002)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0038390 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41941
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18553 Dere\GG16967 Dgri\GH23495 Dmoj\GI22956 Dper\GL22085 Dpse\GA18648 Dsec\GM24273 Dsim\GD19061 Dvir\GJ23772 Dwil\GK14024 Dyak\GE24354
Curated drosophilid orthologs	Dpse\GA18648
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • 187248 Chlamidomonas reinhardtii (green alga) DDB0232004 Dictyostelium discoideum (amoeba) 639804 Populus trichocarpa (black cottonwood) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0241475 FBgn0216030 FBgn0210869 FBgn0190568 FBgn0179137 FBgn0078648 FBgn0159677 FBgn0145683 FBgn0130952 FBgn0109195 FBgn0095571
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	30145 w1118; P{EP}Rbf2G5174
VDRC	v100635 P{KK104541}VIE-260B
Genomic Clones ( 1 )
	BACR04C07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 29 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE32990
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM20956 LD02737 LD15806 RE04262 RE11649 RE17647 RE21660
Other clones	19145 62806 84120.3 175558 180900 CK01.12A.E12 CK01.101C.E10 CK01.101C.H5 Dmel_dig_014071_0624_2556 EK077951 EK085738 EK188345 EK243113 EK243119 EK243137 EK243140 EK243143 EK243146 EK275213 EK280154 RE01657
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038390 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41941
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Rbf2 CG5083 (FlyBase, 1996-)
Additional comments	Source for merge of Rbf2 CG5083 was sequence comparison (date:000407). (FlyBase, 1996-)
Other Comments
	Rbf2 and E2f2 appear to act synergistically to antagonise Rbf-mediated activation, and may co-operate to block S phase progression. (Stevaux et al., 2002)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079648 RefSeq (Proteins) • NP_524372 DDBJ / EMBL / GenBank • AA202554 AA441035 AAF55258 AAF62399 AAF66827 AAL48923 AF195899 AF197059 AY071301 BI236865 Entrez Gene - A searchable database of RefSeq genes. • 41941 UniProtKB/TrEMBL • Q9NIS1 Q9VF04
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0038390 DRSC - Results from RNAi screens. • FBgn0038390 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038390 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5083-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038390 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41941 InParanoid A subset of ortholog calls from InParanoid. • 187248 Chlamidomonas reinhardtii (green alga) DDB0232004 Dictyostelium discoideum (amoeba) 639804 Populus trichocarpa (black cottonwood) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/rbf2.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41941 modMine - Data generated by the modENCODE project. • FBgn0038390 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0241475 FBgn0216030 FBgn0210869 FBgn0190568 FBgn0179137 FBgn0078648 FBgn0159677 FBgn0145683 FBgn0130952 FBgn0109195 FBgn0095571
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG5083 (Beall et al., 2007, Keleman et al., 2009.8.5) RBF2 (Korenjak and Brehm, 2005, Lipsick, 2004, Lee and Orr-Weaver, 2003, Stevaux et al., 2002, Stevaux, 2002.3.12, Douglas et al., 2000, Harbour and Dean, 2000, Tountas and Fortini, 2007, Georlette et al., 2007, Taylor-Harding et al., 2004, Wen et al., 2008, Ambrus et al., 2009, Lee et al., 2010) rbf2 (Dimova et al., 2003, Stevaux, 1999.10.20, Stevaux and Dyson, 2000, Foglietti et al., 2006, Ullah et al., 2007, Buttitta et al., 2007, Wen et al., 2008) Rbf2 (Buckley et al., 2007, Buckley et al., 2006, Keller et al., 2005, Beall et al., 2007, Ullah et al., 2007, Tadros et al., 2007, Ahlander et al., 2008)
Name Synonym	Retinoblastoma-family protein 2 (Stevaux and Dyson, 2000)
Secondary FlyBase IDs
References ( 43 )
Generate a list of	All references relating to this gene ( 43 )
List References by type	Research paper  ( 18 ) Supplementary material  ( 2 ) Review  ( 5 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 5 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Conference report  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Lee et al., 2010, Mol. Cell. Biol. 30(10): 2563--2577 Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. [FBrf0210656] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...