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Gene Dmel\ema

General Information
Symbol	Dmel\ema	Species	D. melanogaster
Name	endosomal maturation defective	Annotation symbol	CG12753
Feature type	protein_coding_gene	FlyBase ID	FBgn0038427
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	89B16-89B16	Sequence location	3R:12,262,602..12,267,382 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene endosomal maturation defective is referred to in FlyBase by the symbol Dmel\ema (CG12753, FBgn0038427). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: endosome to lysosome transport; endosome transport. 4 alleles are reported. The phenotypes of these alleles are annotated with: terminal button; neuromuscular junction; endosome. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Uncharacterised protein family FPL. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1634737_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of CG12753. Gene sequence location is 3R:12262602..12267382.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN21213 HFA14536
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89B16-89B16   Limits computationally determined from genome sequence between P{PZ}gish04895&P{EP}EP3171 and P{lacW}CSN5L4032
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ema for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ema-RA FBtr0083315  NM_142285   3589   1067 ema-RB FBtr0100643  NM_001038969   3382   998
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ema-PA   FBpp0082766   119.4   1067   7.24     NP_650542   AAF55313 ema-PB   FBpp0100104   111.7   998   6.68     NP_001034058   ABC66179
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Uncharacterised protein family FPL (IPR019155)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA697553   AE014297 AAF55313   AE014297 ABC66179   AI389624   AY121652 AAM51979   BG633142   BI161570   BI161571   BI167710   BI239425   BI369430   BP548696   BT021249 AAX33397   CO190494   CO283273   CO308218   CO312991   CO341657   CZ490409   EC058013   EC058026   EC062001   EC066222   EC254766   EC257874   EV579341   GH944301   GH944686   GH949494   GH955713   GH961107   GH962534   GH963261   GH965856   GH969718   GH976950   GH977462   GH979187   GH984237
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q5BIH5 Q8MRD4 Q9VEV4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0038427    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0038427 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during early larval stages, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0038427 expression level Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3979) embryo 02-04hr  (3589) embryo 04-06hr  (3527) embryo 06-08hr  (2337) embryo 08-10hr  (2315) embryo 10-12hr  (1747) embryo 12-14hr  (1778) embryo 14-16hr  (1906) embryo 16-18hr  (1725) embryo 18-20hr  (1519) embryo 20-22hr  (1811) embryo 22-24hr  (1871) larva L1  (1799) larva L2  (1434) larva L3 12hr old  (979) larva L3 puffstage 1-2  (813) larva L3 puffstage 3-6  (1065) larva L3 puffstage 7-9  (1302) white prepupae new  (1307) white prepupae 12hr  (1117) white prepupae 24hr  (1398) pupae 2d postWPP  (1282) pupae 3d postWPP  (1078) pupae 4d postWPP  (1222) adult male 01day  (1353) adult male 05day  (1363) adult male 30day  (1404) adult female 01day  (1956) adult female 05day  (2470) adult female 30day  (2431) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3979) embryo 02-04hr  (3589) embryo 04-06hr  (3527) embryo 06-08hr  (2337) embryo 08-10hr  (2315) embryo 10-12hr  (1747) embryo 12-14hr  (1778) embryo 14-16hr  (1906) embryo 16-18hr  (1725) embryo 18-20hr  (1519) embryo 20-22hr  (1811) embryo 22-24hr  (1871) larva L1  (1799) larva L2  1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day  1404 adult female 01day  (1956) adult female 05day  (2470) adult female 30day  (2431) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0038427 expression level Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3979) embryo 02-04hr  (3589) embryo 04-06hr  (3527) embryo 06-08hr  (2337) embryo 08-10hr  (2315) embryo 10-12hr  (1747) embryo 12-14hr  (1778) embryo 14-16hr  (1906) embryo 16-18hr  (1725) embryo 18-20hr  (1519) embryo 20-22hr  (1811) embryo 22-24hr  (1871) larva L1  (1799) larva L2  (1434) larva L3 12hr old  (979) larva L3 puffstage 1-2  (813) larva L3 puffstage 3-6  (1065) larva L3 puffstage 7-9  (1302) white prepupae new  (1307) white prepupae 12hr  (1117) white prepupae 24hr  (1398) pupae 2d postWPP  (1282) pupae 3d postWPP  (1078) pupae 4d postWPP  (1222) adult male 01day  (1353) adult male 05day  (1363) adult male 30day  (1404) adult female 01day  (1956) adult female 05day  (2470) adult female 30day  (2431) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3979) embryo 02-04hr  (3589) embryo 04-06hr  (3527) embryo 06-08hr  2337 embryo 08-10hr  2315 embryo 10-12hr  1747 embryo 12-14hr  1778 embryo 14-16hr  1906 embryo 16-18hr  1725 embryo 18-20hr  1519 embryo 20-22hr  1811 embryo 22-24hr  1871 larva L1  1799 larva L2  1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day  1404 adult female 01day  1956 adult female 05day  2470 adult female 30day  2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3979 embryo 02-04hr    3589 embryo 04-06hr    3527 embryo 06-08hr    2337 embryo 08-10hr    2315 embryo 10-12hr    1747 embryo 12-14hr    1778 embryo 14-16hr    1906 embryo 16-18hr    1725 embryo 18-20hr    1519 embryo 20-22hr    1811 embryo 22-24hr    1871 larva L1    1799 larva L2    1434 larva L3 12hr old    979 larva L3 puffstage 1-2    813 larva L3 puffstage 3-6    1065 larva L3 puffstage 7-9    1302 white prepupae new    1307 white prepupae 12hr    1117 white prepupae 24hr    1398 pupae 2d postWPP    1282 pupae 3d postWPP    1078 pupae 4d postWPP    1222 adult male 01day    1353 adult male 05day    1363 adult male 30day    1404 adult female 01day    1956 adult female 05day    2470 adult female 30day    2431 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0038427    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0038427 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0038427 expression level Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0038427 expression level Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    61.15 Larval Midgut no informative data Larval Hindgut    42.6 Larval Malpighian Tubules    38.5 Larval Fat Body no informative data Larval Salivary Gland    46.8 Larval Trachea    33.675 Larval Carcass    32.525 Adult Head    56.5 Adult Eye    69.4 Adult Brain    184.3 Adult Thoracic-Abdominal Ganglion    132.2 Adult Crop    40 Adult Midgut no informative data Adult Hindgut    59.8 Adult Malpighian Tubules    70.7 Adult Fat Body    39.9 Adult Salivary Gland no informative data Adult Heart    43.225 Adult VirginFemale Spermatheca    28.6 Adult InseminatedFemale Spermatheca    32 Adult Ovary    73.4 Adult Testis    36.7 Adult Male Accessory Gland no informative data Adult Carcass    72.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    56.5   NA  Eye    69.4   NA  Brain    184.3   61.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    132.2   NA  Crop    40   no informative data  Midgut    no informative data   42.6  Hindgut    59.8   38.5  Malpighian Tubules    70.7   no informative data  Fat Body    39.9   46.8  Salivary Gland    no informative data   NA  Heart    43.225   33.675  Trachea    NA   NA  VirginFemale Spermatheca    28.6   NA  InseminatedFemale Spermatheca    32   NA  Ovary    73.4   NA  Testis    36.7   NA  Male Accessory Gland    no informative data   32.525  Carcass    72.9 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038427 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12753-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | P-stage | recessive emaf07675 viable, with Scer\GAL4pnr-MD237 emaGD474 Other Phenotypes Allele neuroanatomy defective | recessive emaf07675 Phenotype manifest in Allele endosome emaf07675 neuromuscular junction (with Df(3R)Exel7330) emaf07675 terminal button emaf07675 terminal button (with Df(3R)Exel7330) emaf07675
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of ema Class Mutagen Stocks Known lesion emaf07675 amorphic allele - genetic evidence piggyBac transposase 2 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ema Class Mutagen Stocks Known lesion emaGD474 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes emaKK101117 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes emaScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6269 (Ryder, 2004.4.26) Df(3R)Exel7330 (Ryder, 2004.4.26, Kim et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101117} NA P{UAS-ema.GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}emaf07675 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
endosome to lysosome transport inferred from mutant phenotype (Kim et al., 2010) endosome transport inferred from genetic interaction with Vps16A (Kim et al., 2010)
Cellular Component
CV term Evidence References
integral to membrane inferred from direct assay (Kim et al., 2010) colocalizes_with late endosome inferred from direct assay (Kim et al., 2010)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ema allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0038427
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16516 Dere\GG19971 Dgri\GH18244 Dmoj\GI10200 Dper\GL22241 Dpse\GA11790 Dsec\GM15433 Dsim\GD20289 Dvir\GJ23488 Dwil\GK14067 Dyak\GE26313
Curated drosophilid orthologs	Dpse\GA11790
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005902-PA Aedes aegypti (mosquito) AGAP002781-PA Anopheles gambiae (mosquito) XP_393990.2 Apis mellifera (honey bee) XP_001944622.1 Acyrthosiphon pisum (pea aphid) At3g28430.1 Arabidopsis thaliana (wall cress) 183809 Branchiostoma floridae (lancelet) BGIBMGA007095-PA Bombyx mori (silk worm) CN28299 Caenorhabditis brenneri (small nematode) CBP05043 Caenorhabditis briggsae (nematode) CE01183 Caenorhabditis elegans (worm) ENSCAFP00000027911 Canis familiaris (dog) ENSCINP00000011794 Ciona intestinalis (sea squirt) JA18903 Caenorhabditis japonica (nematode) CPIJ008577 Culex pipens (mosquito) ENSCPOP00000002291 Cavia porcellus (guinea pig) RP42961 Caenorhabditis remanei (nematode) ENSCSAVP00000001850 Ciona savignyi (sedentary tunicate) 114708 Capitella spl (polychaete worm) DDB0234043 Dictyostelium discoideum (amoeba) 304370 Daphnia pulex (daphnia) ENSDARP00000094465 Danio rerio (zebra fish) ENSECAP00000018423 Equus caballus (horse) XP_650875 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018966 Gymnopilus aculeatus (mushroom) ENSGALP00000011594 Gallus gallus (chicken) 191365 Hypsipyla robusta (cedar shoot borer) ENSP00000261657 Homo sapiens (human) ISCW015446-PA Ixodes scapularis (deer tick) 196246 Lottia gigantea (owl limpet) 24555 Monocosta brevicollis (choanoflagellate) ENSMODP00000006363 Monodelphis domestica (gray opossum) ENSMMUP00000008579 Macaca mulatta (rhesus monkey) ENSMUSP00000065423 Mus musculus (house mouse) 84933 Nematostella vectensis (starlet anemone) ENSOANP00000001161 Ornithorynchus anatinus (platypus) ENSORLP00000025842 Oryzias latipes (japanese killifish) PHUM003591-PA Pediculus humanus (human body louse) PP25023 Pristionchus pacificus (nematode) 188232 Physcomitrella patens (moss) ENSPPYP00000008027 Pongo pygmaeus (orangutan) 127538 Phytophthora sojae (root rot pathogen) 826046 Populus trichocarpa (black cottonwood) ENSPTRP00000053770 Pan troglodytes (chimpanzee) PVX_114075 Plasmodium vivax (protozoal parasite) ENSRNOP00000003541 Rattus norvegicus (norway rat) Smp_143110 Schistosoma mansoni (trematode parasite) XP_001176503.1 Strongylocentrotus purpuratus (purple sea urchin) 53756 Trichoplax adhaerens (metazoa) TA14350 Theileria annulata (apicomplexan parasite) XP_974210.1 Tribolium castaneum (red flour beetle) Tc00.1047053507007.20 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053509551.130 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000014561 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000026660 Takifugu rubripes (pufferfish Takifugu) 170.m00055 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000051989 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50163 LH22511 ISCW015446 FBgn0243343 FBgn0216073 FBgn0210586 FBgn0191764 FBgn0170351 JGI_V11_304370 FBgn0071839 FBgn0159833 FBgn0132966 FBgn0125712 FBgn0112171 FBgn0093537 CPIJ008577 BGIBMGA007095 ACYPI010161 GB17939 AGAP002781 AAEL005902 PB22363 PHUM184150 GLEAN_05512
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	19092 w1118; PBac{WH}emaf07675/TM6B, Tb1
Harvard	- PBac{WH}emaf07675
VDRC	v1617 w1118; P{GD474}v1617 v1618 w1118; P{GD474}v1618/CyO
Genomic Clones ( 1 )
	BACR01H23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE01053 RE60631
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL02759 RE08154 RE35909 RE55612
Other clones	43420 153492 214475.32 214577.22 CK01.59A.A2 CK01.59D.H6 Dmel_dig_017857_0652_3228 EK048363 EK162742 EK230904 EK257025 EP12912 FGM001C08 GH20847 RP004092441 RP004184757 RP004200368 RP004238926 RP004242441 RP004254542 RP004290266 RP004306058 RP004437094 RP004487079 RP004516144 RP004524423 RP004539001
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038427 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41992
Antibody Information
	polyclonal (Kim et al., 2010)
Other Information
Discoverer
Etymology
	The name 'endosomal maturation defective' is based on the loss-of-function phenotype of the gene. (Kim et al., 2010)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ema CG12753 (Kim et al., 2010)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142285 NM_001038969 RefSeq (Proteins) • NP_650542 NP_001034058 DDBJ / EMBL / GenBank • AAF55313 AAF55313.2 AAM51979 AAX33397 ABC66179 AY121652 BI161570 BI161571 BT021249 CZ490409 Entrez Gene - A searchable database of RefSeq genes. • 41992 UniProtKB/TrEMBL • Q5BIH5 Q8MRD4 Q9VEV4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Uncharacterised protein family FPL (IPR019155)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0038427 DRSC - Results from RNAi screens. • FBgn0038427 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038427 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12753-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038427 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41992 InParanoid A subset of ortholog calls from InParanoid. • AAEL005902-PA Aedes aegypti (mosquito) AGAP002781-PA Anopheles gambiae (mosquito) XP_393990.2 Apis mellifera (honey bee) XP_001944622.1 Acyrthosiphon pisum (pea aphid) At3g28430.1 Arabidopsis thaliana (wall cress) 183809 Branchiostoma floridae (lancelet) BGIBMGA007095-PA Bombyx mori (silk worm) CN28299 Caenorhabditis brenneri (small nematode) CBP05043 Caenorhabditis briggsae (nematode) CE01183 Caenorhabditis elegans (worm) ENSCAFP00000027911 Canis familiaris (dog) ENSCINP00000011794 Ciona intestinalis (sea squirt) JA18903 Caenorhabditis japonica (nematode) CPIJ008577 Culex pipens (mosquito) ENSCPOP00000002291 Cavia porcellus (guinea pig) RP42961 Caenorhabditis remanei (nematode) ENSCSAVP00000001850 Ciona savignyi (sedentary tunicate) 114708 Capitella spl (polychaete worm) DDB0234043 Dictyostelium discoideum (amoeba) 304370 Daphnia pulex (daphnia) ENSDARP00000094465 Danio rerio (zebra fish) ENSECAP00000018423 Equus caballus (horse) XP_650875 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000018966 Gymnopilus aculeatus (mushroom) ENSGALP00000011594 Gallus gallus (chicken) 191365 Hypsipyla robusta (cedar shoot borer) ENSP00000261657 Homo sapiens (human) ISCW015446-PA Ixodes scapularis (deer tick) 196246 Lottia gigantea (owl limpet) 24555 Monocosta brevicollis (choanoflagellate) ENSMODP00000006363 Monodelphis domestica (gray opossum) ENSMMUP00000008579 Macaca mulatta (rhesus monkey) ENSMUSP00000065423 Mus musculus (house mouse) 84933 Nematostella vectensis (starlet anemone) ENSOANP00000001161 Ornithorynchus anatinus (platypus) ENSORLP00000025842 Oryzias latipes (japanese killifish) PHUM003591-PA Pediculus humanus (human body louse) PP25023 Pristionchus pacificus (nematode) 188232 Physcomitrella patens (moss) ENSPPYP00000008027 Pongo pygmaeus (orangutan) 127538 Phytophthora sojae (root rot pathogen) 826046 Populus trichocarpa (black cottonwood) ENSPTRP00000053770 Pan troglodytes (chimpanzee) PVX_114075 Plasmodium vivax (protozoal parasite) ENSRNOP00000003541 Rattus norvegicus (norway rat) Smp_143110 Schistosoma mansoni (trematode parasite) XP_001176503.1 Strongylocentrotus purpuratus (purple sea urchin) 53756 Trichoplax adhaerens (metazoa) TA14350 Theileria annulata (apicomplexan parasite) XP_974210.1 Tribolium castaneum (red flour beetle) Tc00.1047053507007.20 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053509551.130 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000014561 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000026660 Takifugu rubripes (pufferfish Takifugu) 170.m00055 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000051989 Xenopus tropicalis (frog) modMine - Data generated by the modENCODE project. • FBgn0038427 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV50163 LH22511 ISCW015446 FBgn0243343 FBgn0216073 FBgn0210586 FBgn0191764 FBgn0170351 JGI_V11_304370 FBgn0071839 FBgn0159833 FBgn0132966 FBgn0125712 FBgn0112171 FBgn0093537 CPIJ008577 BGIBMGA007095 ACYPI010161 GB17939 AGAP002781 AAEL005902 PB22363 PHUM184150 GLEAN_05512
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG12753 (Kim et al., 2010, Keleman et al., 2009.8.5) ema (Kim et al., 2010)
Name Synonym	endosomal maturation defective (Kim et al., 2010)
Secondary FlyBase IDs
References ( 12 )
Research paper	Kim et al., 2010, J. Cell Biol. 188(5): 717--734 The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. [FBrf0210177] Matzkin et al., 2005, Genetics 170(3): 1143--1152 The structure and population genetics of the breakpoints associated with the cosmopolitan chromosomal inversion In(3R)Payne in Drosophila melanogaster. [FBrf0190746]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]