FB2025_01 , released February 20, 2025
Gene: Dmel\Ns1
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General Information
Symbol
Dmel\Ns1
Species
D. melanogaster
Name
Nucleostemin 1
Annotation Symbol
CG3983
Feature Type
protein_coding_gene
FlyBase ID
FBgn0038473
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Nucleostemin 1 (Ns1) encodes a GTPase that is enriched in the peripheral granular components of nucleoli of most larval and adult cells. It is required for nucleolar release of the large ribosomal subunit. Depletion of the Ns1 product reduces viability of midgut imaginal island cells and ribosome abundance in polyploid cells. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
89E11-89E11
Sequence location
Recombination map
3-59
RefSeq locus
NT_033777 REGION:17070523..17072675
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables ATP hydrolysis activity
inferred from direct assay
(Rosby et al., 2009)
enables GTPase activity
inferred from direct assay
(Rosby et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables GTP binding
inferred from electronic annotation with InterPro:IPR006073, InterPro:IPR030378
(InterPro Project Members, 2004-)
inferred from sequence or structural similarity with UniProtKB:Q811S9
(Gene Ontology Curators, 2002-)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in regulation of cell population proliferation
inferred from mutant phenotype
(Kaplan et al., 2008)
involved_in regulation of multicellular organism growth
inferred from mutant phenotype
(Kaplan et al., 2008)
involved_in ribosomal large subunit biogenesis
inferred from mutant phenotype
(Rosby et al., 2009)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in granular component
inferred from direct assay
(Rosby et al., 2009)
located_in nucleolus
inferred from direct assay
(Moutinho-Pereira et al., 2013, Kaplan et al., 2008)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in nucleolus
inferred from biological aspect of ancestor with PANTHER:PTN000119956
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TRAFAC class YlqF/YawG GTPase family. (Q8MT06)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
GTPase activity
RHEA 19669: ATP hydrolysis activity
RHEA 13065:
Summaries
Gene Snapshot
Nucleostemin 1 (Ns1) encodes a GTPase that is enriched in the peripheral granular components of nucleoli of most larval and adult cells. It is required for nucleolar release of the large ribosomal subunit. Depletion of the Ns1 product reduces viability of midgut imaginal island cells and ribosome abundance in polyploid cells. [Date last reviewed: 2018-11-15]
Gene Group (FlyBase)
YLQF/YAWG GTPASES -
The YlqF/YawG GTPases are a family of GTPases characterized by a circularly permuted order of GTPase motifs within the GTP-binding domain. These are involved in ribosome biogenesis, cell proliferation, or cell growth. (Adapted from FBrf0209157 and PMID:21205822).
UNCLASSIFIED ATPASES -
This group comprises ATPases that do not classify under other groups in FlyBase.
Protein Function (UniProtKB)
May play a role in regulating cellular proliferation.
(UniProt, Q8MT06)
Summary (Interactive Fly)

GTPase that acts in serotonergic neurons to regulate insulin signaling and thus exert global growth control

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Ns1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q8MT06)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Ns1-RA
FBtr0083426
NM_169742
1895
581
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Ns1-PA
FBpp0082867
66.0
581
10.02
Q8MT06
NP_732199
AAF55384
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Domain

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

(UniProt, Q8MT06)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ns1 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.64

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in granular component
inferred from direct assay
(Rosby et al., 2009)
located_in nucleolus
inferred from direct assay
(Moutinho-Pereira et al., 2013, Kaplan et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ns1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ns1
Transgenic constructs containing regulatory region of Ns1
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(3)DadP1883Δ32
(Bieli et al., 2015)
Df(3R)Exel6176
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (7)
Hsap\GNL3L
12 of 14
Yes
Yes
Hsap\GNL3
7 of 14
No
Yes
Hsap\GNL2
3 of 14
No
No
Hsap\GNL1
2 of 14
No
No
Hsap\LSG1
2 of 14
No
No
Hsap\MTG1
2 of 14
No
No
Hsap\NOA1
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (7)
Rnor\Gnl3l
12 of 14
Yes
Yes
Rnor\Gnl3
7 of 14
No
Yes
Rnor\Gnl2
3 of 14
No
No
Rnor\Gnl1
2 of 14
No
No
Rnor\Lsg1
2 of 14
No
No
Rnor\Mtg1
2 of 14
No
No
Rnor\Noa1
1 of 14
No
No
Mus musculus (laboratory mouse) (7)
Mmus\Gnl3l
12 of 14
Yes
Yes
Mmus\Gnl3
7 of 14
No
Yes
Mmus\Gnl1
2 of 14
No
No
Mmus\Gnl2
2 of 14
No
No
Mmus\Lsg1
2 of 14
No
No
Mmus\Mtg1
2 of 14
No
No
Mmus\Noa1
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\gnl3l
7 of 13
Yes
No
Xtro\gnl3
6 of 13
No
Yes
Xtro\acss1
1 of 13
No
No
Xtro\gnl2
1 of 13
No
No
Xtro\lsg1
1 of 13
No
No
Xtro\mtg1
1 of 13
No
No
Xtro\usp10
1 of 13
No
No
Danio rerio (Zebrafish) (7)
Drer\gnl3l
13 of 14
Yes
Yes
Drer\gnl3
8 of 14
No
Yes
Drer\gnl1
2 of 14
No
No
Drer\gnl2
2 of 14
No
No
Drer\mtg1
2 of 14
No
No
Drer\drg1
1 of 14
No
No
Drer\lsg1
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\nst-1
14 of 14
Yes
Yes
Cele\ngp-1
2 of 14
No
No
Cele\C53H9.2
1 of 14
No
No
Cele\mtg-1
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (6)
Agam\AgaP_AGAP004514
12 of 12
Yes
Yes
Agam\AgaP_AGAP011309
3 of 12
No
No
Agam\AgaP_AGAP004736
2 of 12
No
No
Agam\AgaP_AGAP008330
2 of 12
No
No
Agam\AgaP_AGAP011471
2 of 12
No
No
Agam\AgaP_AGAP010271
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (12)
Atha\NSN1
12 of 13
Yes
Yes
Atha\AtNug2
3 of 13
No
No
Atha\AT1G08410
2 of 13
No
No
Atha\AT2G27200
2 of 13
No
No
Atha\AT2G41670
2 of 13
No
No
Atha\AT4G10650
2 of 13
No
No
Atha\BPG2
2 of 13
No
No
Atha\EMB3129
2 of 13
No
No
Atha\AT1G72660
1 of 13
No
No
Atha\AT4G10620
1 of 13
No
No
Atha\DRG1
1 of 13
No
No
Atha\NOA1
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\NUG1
11 of 13
Yes
Yes
Scer\LSG1
2 of 13
No
No
1  
Scer\MTG1
2 of 13
No
No
Scer\NOG2
2 of 13
No
No
Scer\GEP3
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\grn1
10 of 12
Yes
Yes
Spom\mtg1
2 of 12
No
No
Spom\SPAC3F10.16c
2 of 12
No
No
Spom\SPAC6F6.03c
2 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\der
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Ns1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (5)
Ns2
6 of 13
Ns3
4 of 13
Ns4
4 of 13
CG17141
3 of 13
CG10914
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    GNL3L; G protein nucleolar 3 like
    12 of 14
    300873
        GNL3; G protein nucleolar 3
        7 of 14
        608011
            GNL2; G protein nucleolar 2
            3 of 14
            609365
                Functional Complementation Data
                Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                Interactions
                Summary of Physical Interactions
                esyN Network Diagram
                Show neighbor-neighbor interactions:
                Show/hide secondary interactors 
                (data from AllianceMine provided by esyN)
                Select Layout:
                Legend:
                Protein
                RNA
                Selected Interactor(s)
                Other Interaction Browsers
                View in FlyBase Interactions Browser   View in MIST tool (external link)
                Please see the Physical Interaction reports below for full details
                protein-protein
                Physical Interaction
                Assay
                References
                Ns1 - CG32344
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - CG6937
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - CG8939
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - Ifrd1
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - Non1
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - Nop56
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - Rs1
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - S6kII
                anti tag coimmunoprecipitation, Identification by mass spectrometry
                (Vinayagam et al., 2016)
                Ns1 - Srp72
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - Tsc1
                anti tag coimmunoprecipitation, Identification by mass spectrometry
                (Vinayagam et al., 2016)
                Ns1 - gig
                anti tag coimmunoprecipitation, Identification by mass spectrometry
                (Vinayagam et al., 2016)
                Ns1 - l(1)G0020
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - nop5
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - pit
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - pths
                experimental knowledge based
                (Guruharsha et al., 2011)
                Ns1 - rictor
                anti tag coimmunoprecipitation, Identification by mass spectrometry
                (Vinayagam et al., 2016)
                Summary of Genetic Interactions
                esyN Network Diagram
                Other Interaction Browsers
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                Starting gene(s)
                Interaction type
                Interacting gene(s)
                Reference
                External Data
                Linkouts
                DroID - A comprehensive database of gene and protein interactions.
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Pathways
                Signaling Pathways (FlyBase)
                Metabolic Pathways
                FlyBase
                External Links
                External Data
                Linkouts
                KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                Class of Gene
                Genomic Location and Detailed Mapping Data
                Chromosome (arm)
                3R
                Recombination map
                3-59
                Cytogenetic map
                89E11-89E11
                Sequence location
                FlyBase Computed Cytological Location
                Cytogenetic map
                Evidence for location
                89E11-89E11
                Limits computationally determined from genome sequence between P{EP}CG5208EP562&P{EP}DadEP3196 and P{PZ}l(3)0788207882
                Experimentally Determined Cytological Location
                Cytogenetic map
                Notes
                References
                Experimentally Determined Recombination Data
                Location

                3-59

                (FlyBase, 2016)

                3-59.0

                (Comeron et al., 2012)
                Left of (cM)
                Right of (cM)
                Notes
                Stocks and Reagents
                Stocks (8)
                Genomic Clones (10)
                 

                Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

                cDNA Clones (167)
                 

                Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

                cDNA clones, fully sequenced
                BDGP DGC clones
                Other clones
                Drosophila Genomics Resource Center cDNA clones

                For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                cDNA Clones, End Sequenced (ESTs)
                BDGP DGC clones
                Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                Antibody Information
                Laboratory Generated Antibodies
                 

                polyclonal

                (Rosby et al., 2009)
                Commercially Available Antibodies
                 
                Cell Line Information
                Publicly Available Cell Lines
                 
                  Other Stable Cell Lines
                   
                    Other Comments

                    dsRNA made from templates generated with primers directed against this gene results in a change in cell proliferation and cell size.

                    (Guertin et al., 2006)
                    Relationship to Other Genes
                    Source for database merge of
                    Additional comments
                    Nomenclature History
                    Source for database identify of

                    Source for identity of: ns1 CG3983

                    (Kaplan et al., 2008)

                    Source for identity of: Ns1 ns1

                    (FlyBase, 2009-)
                    Nomenclature comments
                    Etymology

                    Named "Ns1" after the human ortholog.

                    (FlyBase, 2009-)
                    Synonyms and Secondary IDs (9)
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    Study result (0)
                    Result
                    Result Type
                    Title
                    External Crossreferences and Linkouts ( 36 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                    Other crossreferences
                    AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                    FlyMine - An integrated database for Drosophila genomics
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    MARRVEL_MODEL - MARRVEL (model organism gene)
                    Linkouts
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    References (64)