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Gene Dmel\TwdlW

General Information
Symbol	Dmel\TwdlW	Species	D. melanogaster
Name	TweedleW	Annotation symbol	CG4060
Feature type	protein_coding_gene	FlyBase ID	FBgn0038487
Gene Model Status	Current	Stock availability	3 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	89F1-89F1	Sequence location	3R:12,966,930..12,968,126 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TweedleW is referred to in FlyBase by the symbol Dmel\TwdlW (CG4060, FBgn0038487). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: structural constituent of chitin-based cuticle. Based on sequence similarity, it is predicted to be involved in the biological process: chitin-based cuticle development; body morphogenesis. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Domain of unknown function DUF243. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 12-24 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1637568_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TwdlW. Gene sequence location is 3R:12966930..12968126.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89F1-89F1   Limits computationally determined from genome sequence between P{EP}CG5208EP562&P{EP}DadEP3196 and P{PZ}l(3)0788207882
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TwdlW for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TwdlW-RA FBtr0083415  NM_142349   1126   308
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TwdlW-PA   FBpp0082856   34.2   308   6.52     NP_650606   AAF55398
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA735521   AE014297 AAF55398   AI455147   BI214627   BI239798   BT001478 AAN71233   CO187760   CO310974   CO311676   EC206608   EC213643   GH748005   GH748420   GH748609   GH748709   GH749719   GH755427   GH755588   GH757733   GH758797   GH759102   GH759775   GH759777   GH760207   GH760390   GH760771   GH775826   GH776035   GH778817   GH781549   GH783250   GH786742   GH788571   GH789090   GH789340   GH789402   GH790046   GH790509   GH790770   GH791591   GH791964   GH791996   GH792532   GH792933   GH794030   GH795091   GH795305   GH796100   GH797434   GH798450   GH799262   GH800734   GH800805   GH800961   GH801378   GH803452   GH807845   GH808703   GH808885   GH809355   GH810292   GH810330   GH810642   GH812466   GH813279   GH813954   GH814109   GH814756   GH816003   GH816021   GH816046   GH816265   GH818736   GH819683   GH822913   GH823415   GH823881   GH827662   GH829667   GH830437   GH832394   GH832500   GH835352   GH835585   GH838858   GH839449   GH839489   GH839626   GH840275   GH840546   GH840662   GH840769   GH844186   GH845634   GH847871   GH849058   GH852382   GH852897   GH853913   GH855568   GH856829   GH856891   GH857441   GH857470   GH859491   GH860026   GH863052   GH863629   GH864416   GH865224   GH866883   GH867333   GH867799   GH869029   GH870383   GH871739   GH872864   GH873286   GH873469   GH877512   GH877765   GH879691   GH881605   GH881822   GH881947   GH883126   GH884961   GH886100   GH886424   GH887142   GH888423   GH889001   GH889254   GH889610   GH891218   GH893674   GH896066   GH896697   GH899404   GH902397   GH903249   GH906036   GH907752   GH907849   GH909289   GH911118   GH912153   GH912521   GH914059   GH915013   GH919088   GH919908   GH921197   GH921315   GH922442   GH922753   GH922843   GH923189   GH923272   GH923333   GH923678   GH924120   GH925207   GH925710   GH925796   GH926128   GH926456   GH928159   GH931227   GH934518   GH934885   GH935733   GH935883   GH935911   GH936534   GH936860   GH937019   GH937547   GH937653   GH938208   GH939304   GH939420   GH939453   GH940192   GH940209   GH940221   GH940840
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IH13
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.275 Larval Midgut    1.5 Larval Hindgut    3.6 Larval Malpighian Tubules    4.5 Larval Fat Body    1.9 Larval Salivary Gland    0.8 Larval Trachea    4.3 Larval Carcass    7.6 Adult Head    3.6 Adult Eye    3.125 Adult Brain    16.1 Adult Thoracic-Abdominal Ganglion    17.1 Adult Crop    5 Adult Midgut    2.3 Adult Hindgut    4.4 Adult Malpighian Tubules    1.3 Adult Fat Body    7.1 Adult Salivary Gland    11.2 Adult Heart    1.8 Adult VirginFemale Spermatheca    4.9 Adult InseminatedFemale Spermatheca    2.3 Adult Ovary    1.7 Adult Testis    4.6 Adult Male Accessory Gland    5.3 Adult Carcass    4.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.6   NA  Eye    3.125   NA  Brain    no informative data   3.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    5   1.5  Midgut    2.3   3.6  Hindgut    4.4   4.5  Malpighian Tubules    1.3   1.9  Fat Body    7.1   0.8  Salivary Gland    no informative data   NA  Heart    1.8   4.3  Trachea    NA   NA  VirginFemale Spermatheca    4.9   NA  InseminatedFemale Spermatheca    2.3   NA  Ovary    1.7   NA  Testis    4.6   NA  Male Accessory Gland    5.3   7.6  Carcass    4.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    1 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    0 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 12-24 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr    102 embryo 16-18hr    696 embryo 18-20hr    491 embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2    159 larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr  (102) embryo 16-18hr  (696) embryo 18-20hr  (491) embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2  (159) larva L3 12hr old  (99) larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr  102 embryo 16-18hr  (696) embryo 18-20hr  (491) embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2  (159) larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr    102 embryo 16-18hr    696 embryo 18-20hr    491 embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2    159 larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr    102 embryo 16-18hr    696 embryo 18-20hr    491 embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2    159 larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr  (102) embryo 16-18hr  (696) embryo 18-20hr  (491) embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2  (159) larva L3 12hr old  (99) larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr  102 embryo 16-18hr  (696) embryo 18-20hr  (491) embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2  (159) larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    3 embryo 10-12hr    2 embryo 12-14hr    3 embryo 14-16hr    102 embryo 16-18hr    696 embryo 18-20hr    491 embryo 20-22hr    14 embryo 22-24hr    18 larva L1    2 larva L2    159 larva L3 12hr old    99 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    0 white prepupae 24hr    0 pupae 2d postWPP    2 pupae 3d postWPP    1 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    0 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    0 wing disc ML-DmD8    0 wing disc ML-DmD9    1 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    1 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    0 ovary OSC    0 ovary OSS    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    4 Paraquat 10 mM 48 hrs, 4-day adult    7 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_12 mE1_20_mRNA_expression_cluster_12
External Data & Images
Linkouts	BDGP expression data • LD21452 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038487 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4060-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TwdlW Stages(s) 1-3 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 TwdlWGD11204 Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of TwdlW Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TwdlW Class Mutagen Stocks Known lesion TwdlWGD11204 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes TwdlWKK110352 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6176 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK110352} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}EY02138 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
structural constituent of chitin-based cuticle inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Biological Process
CV term Evidence References
body morphogenesis inferred from sequence or structural similarity with TwdlD (Curators, 2008--) chitin-based cuticle development inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Cellular Component
CV term Evidence References
proteinaceous extracellular matrix inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TwdlW allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67115 DroID - A comprehensive database of gene and protein interactions. • FBgn0038487 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42075
Orthologs
OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6ZW5XH Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6ZW5XH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\TwdlW     Drosophila simulans   Dsim\GD20247 Y   Drosophila sechellia   Dsec\GM15381 Y   Drosophila erecta   Dere\GG16788 Y   Drosophila yakuba   Dyak\GE24178 Y   Drosophila ananassae   Dana\GF17860 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17925 Y   Drosophila persimilis   Dper\GL27212 Y   Drosophila willistoni   Dwil\GK10882 Y   Drosophila virilis   Dvir\GJ10443 Y   Drosophila mojavensis   Dmoj\GI23141 Y   Drosophila grimshawi   Dgri\GH14248 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD20247 Drosophila sechellia Dsec\GM15381 Drosophila erecta Dere\GG16788 Drosophila yakuba Dyak\GE24178 Drosophila ananassae Dana\GF17860 Drosophila pseudoobscura pseudoobscura Dpse\GA17925 Drosophila persimilis Dper\GL27212 Drosophila willistoni Dwil\GK10882 Drosophila virilis Dvir\GJ10443 Drosophila mojavensis Dmoj\GI23141 Drosophila grimshawi Dgri\GH14248
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
VDRC	v101966 P{KK110352}VIE-260B v26380 w1118; P{GD11204}v26380/CyO v26381 w1118; P{GD11204}v26381/CyO
Genomic Clones ( 2 )
	BACR01E04 BACR02L16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 184 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD21452
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE20593 RE36385
Other clones	102463 CK01.39A.F10 EK032855 EK223101 EK225816 RP003005755 RP003009368 RP003014827 RP003018806 RP003030305 RP003032126 RP003039363 RP003047286 RP003053738 RP003067991 RP003069047 RP003071072 RP003071604 RP003074261 RP003075474 RP003083486 RP003086569 RP003089625 RP003095613 RP003097147 RP003099469 RP003110668 RP003110930 RP003119070 RP003130852 RP003131278 RP003132294 RP003139403 RP003142879 RP003143227 RP003150897 RP003152475 RP003161213 RP003163938 RP003168688 RP003170620 RP003171208 RP003173048 RP003174210 RP003174374 RP003177539 RP003179591 RP003193354 RP003199612 RP003199906 RP003200665 RP003203829 RP003217232 RP003217889 RP003218300 RP003222449 RP003222900 RP003228099 RP003228986 RP003230099 RP003235818 RP003243823 RP003253356 RP003259432 RP003269729 RP003270168 RP003271070 RP003277660 RP003281201 RP003283396 RP003294425 RP003299781 RP003301261 RP003304526 RP003305317 RP003307842 RP003311984 RP003313227 RP003313816 RP003329038 RP003330179 RP003331188 RP003331270 RP003333826 RP003337622 RP003337759 RP003338315 RP003342704 RP003344273 RP003346122 RP003346437 RP003346585 RP003347532 RP003355888 RP003360939 RP003363481 RP003367932 RP003375784 RP003376286 RP003386865 RP003389716 RP003393274 RP003406950 RP003420334 RP003422179 RP003426354 RP003435135 RP003436905 RP003447950 RP003450547 RP003452282 RP003453937 RP003454057 RP003468841 RP003469124 RP003471419 RP003472869 RP003478074 RP003478649 RP003480213 RP003487250 RP003493277 RP003499083 RP003504232 RP003508378 RP003509590 RP003510813 RP003511224 RP003511490 RP003513046 RP003515041 RP003515680 RP003522122 RP003522162 RP003526825 RP003526835 RP003532289 RP003532339 RP003534116 RP003534144 RP003534961 RP003539843 RP003543198 RP003543924 RP003552628 RP003553293 RP003554629 RP003557016 RP003557518 RP003560646 RP003560806 RP003561213 RP003563024 RP003564193 RP003564937 RP003567590 RP003567990 RP003571054 RP003586460 RP003589331 RP003590403 RP003607831 RP003609168 RP003611477 RP003612249 RP003614381 RP003620731 RP003634490 RP003635111 RP003635112 RP003637934 RP003645460 RP003649650 RP003652522 RP003652912 RP003653257 RP003658281 RP003659887 RP003660093 RP003662912 RP003669892
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038487 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42075
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: TwdlW CG4060 (Guan, 2006)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142349 RefSeq (Proteins) • NP_650606 DDBJ / EMBL / GenBank • AA735521 AAF55398 AAN71233 AI455147 BT001478 Entrez Gene - A searchable database of RefSeq genes. • 42075 UniProtKB/TrEMBL • Q8IH13
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD21452
Linkouts
	BioGRID - A database of protein and genetic interactions • 67115 DroID - A comprehensive database of gene and protein interactions. • FBgn0038487 DRSC - Results from RNAi screens. • FBgn0038487 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038487 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4060-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038487 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42075 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42075 modMine - Data generated by the modENCODE project. • FBgn0038487
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG4060 (Guan, 2006, Keleman et al., 2009.8.5, Haussmann et al., 2008) TwdlW (Guan, 2006)
Name Synonym	CG4060   TweedleW (Cornman, 2010)
Secondary FlyBase IDs
References ( 15 )
Research paper	Cornman, 2010, PLoS ONE 5(9): e12536 The Distribution of GYR- and YLP-Like Motifs in Drosophila Suggests a General Role in Cuticle Assembly and Other Protein-Protein Interactions. [FBrf0211774] Haussmann et al., 2008, Genome Biol. 9(4): R73 Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways. [FBrf0209726]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Guan, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(45): [FBrf0200374] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335] Gene Disruption Project members, 2001-, (Computer file) (Computer file) [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433] Curators, 2008--, Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. [FBrf0202953]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]