FB2021_01, released Feb 18, 2021

Gene: Dmel\Non3

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General Information
Symbol
Dmel\Non3
Species
D. melanogaster
Name
Novel nucleolar protein 3
Annotation Symbol
CG7993
Feature Type
protein_coding_gene
FlyBase ID
FBgn0038585
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Key Links
Genomic Location
Cytogenetic map
90F8-90F8
Sequence location
3R:18,223,722..18,224,942 [+]
Recombination map

3-62

RefSeq locus
NT_033777 REGION:18223722..18224942
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables rRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000296192
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
inferred from biological aspect of ancestor with PANTHER:PTN000296192
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in ribosomal large subunit assembly
inferred from biological aspect of ancestor with PANTHER:PTN000296192
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in nucleolus
inferred from direct assay
(Moutinho-Pereira et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in nucleolus
inferred from biological aspect of ancestor with PANTHER:PTN000296192
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the RPF2 family. (Q9VEB3)
Protein Signatures (InterPro)
Summaries
Protein Function (UniProtKB)
Required for normal assembly of the mitotic spindle. May be involved in both centrosome-dependent and centrosome-independent spindle assembly programs.
(UniProt, Q9VEB3)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Non3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Non3-RA
FBtr0083562
NM_142437
1093
320
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Non3-PA
FBpp0082984
36.5
320
11.00
NP_650694
AAF55514
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Non3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous

Comment: apically cleared

(Fisher et al., 2012)
yolk
(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
hindgut anlage
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anal pad primordium

Comment: reported as anal pad specific anlage

(Fisher et al., 2012)
anterior midgut primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
longitudinal visceral muscle primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)
somatic muscle primordium
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
visceral muscle primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
circular visceral muscle fiber
(Fisher et al., 2012)
dorsal pharyngeal muscle
(Fisher et al., 2012)
embryonic anal pad
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
embryonic/larval visceral muscle
(Fisher et al., 2012)
longitudinal visceral muscle fiber
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleolus
inferred from direct assay
(Moutinho-Pereira et al., 2013)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Non3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Non3
Transgenic constructs containing regulatory region of Non3
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation
Gene disrupted in
Df(3R)Exel6178
(Ryder, 2004.4.26)
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal - all die before end of first instar larval stage
lethal - all die before end of P-stage (with Non3197)
lethal - all die before end of P-stage (with Non3259)
lethal - all die before end of P-stage (with Non3310)
lethal - all die before end of P-stage (with Non3G4706)
lethal - all die before end of P-stage (with Non3Δ600)
lethal - all die before end of P-stage | recessive
lethal - all die during larval stage, with Scer\GAL4Mef2.PR
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Scer\GAL4tin.CΔ4
viable (with Non3197)
viable (with Non3259)
viable (with Non3310)
viable (with Non3G4706)
Sterility
Allele
fertile
fertile (with Non3197)
fertile (with Non3259)
fertile (with Non3310)
semi-sterile
semi-sterile (with Non3197)
semi-sterile (with Non3310)
semi-sterile (with Non3G4706)
semi-sterile (with Non3Δ600)
sterile (with Non3259)
sterile (with Non3G4706)
Other Phenotypes
Allele
abnormal neuroanatomy | larval stage
abnormal size | adult stage (with Non3197)
abnormal size | adult stage (with Non3259)
abnormal size | adult stage (with Non3310)
abnormal size | adult stage (with Non3G4706)
abnormal size | adult stage (with Non3Δ600)
abnormal size | adult stage | recessive
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
visible | adult stage (with Non3197)
visible | adult stage (with Non3259)
visible | adult stage (with Non3310)
visible | adult stage (with Non3G4706)
visible | adult stage (with Non3Δ600)
visible | adult stage | recessive
Phenotype manifest in
Allele
adult thoracic mechanosensory chaeta
adult thoracic mechanosensory chaeta (with Non3197)
adult thoracic mechanosensory chaeta (with Non3259)
adult thoracic mechanosensory chaeta (with Non3310)
adult thoracic mechanosensory chaeta (with Non3G4706)
adult thoracic mechanosensory chaeta (with Non3Δ600)
embryonic/larval somatic muscle, with Scer\GAL4Mef2.PR
ganglion mother cell | larval stage
larval neuroblast | larval stage
mesothoracic tergum, with Scer\GAL4pnr-MD237
trichogen cell, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RPF2
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rpf2
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Rpf2
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\rpf2
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\rpf2
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\Y54E10A.10
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AT3G23620
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\RPF2
14 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC926.08c
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190DC0 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00009788
Drosophila simulans
Dsim\GD19216
Drosophila sechellia
Dsec\GM15289
Drosophila erecta
Dere\GG22748
Drosophila yakuba
Dyak\GE25522
Drosophila ananassae
Dana\GF23070
Drosophila pseudoobscura pseudoobscura
Dpse\GA20749
Drosophila persimilis
Dper\GL12003
Drosophila willistoni
Dwil\GK22384
Drosophila virilis
Dvir\GJ22542
Drosophila mojavensis
Dmoj\GI24755
Drosophila grimshawi
Dgri\GH18556
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506VC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17015688
Glossina morsitans
Tsetse fly
Gmor\GMOY006798
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_13570
Mayetiola destructor
Hessian fly
Mdes\Mdes003324
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL004455
Anopheles darlingi
American malaria mosquito
Adar\ADAC005542
Anopheles gambiae
Malaria mosquito
Agam\AGAP003048
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ014360
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0C83 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA011092
Danaus plexippus
Monarch butterfly
Dple\DPOGS203208-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL010980
Apis florea
Little honeybee
Aflo\4299170340
Apis mellifera
Western honey bee
Amel\LOC551311
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11970
Bombus terrestris
Buff-tailed bumblebee
Bter\15216824
Linepithema humile
Argentine ant
Lhum\LH12371
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00300c
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003035
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08648
Tribolium castaneum
Red flour beetle
Tcas\TC011042
Pediculus humanus
Human body louse
Phum\PHUM430960
Rhodnius prolixus
Kissing bug
Rpro\RPRC006277
Cimex lectularius
Bed bug
Clec\CLEC002749
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003681
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_00258
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0C5U )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR005182
Strigamia maritima
European centipede
Smar\SMAR005180
Ixodes scapularis
Black-legged tick
Isca\ISCW015558
Stegodyphus mimosarum
African social velvet spider
Smim\KFM81104.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03540
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g03510
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_302434
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0EYG )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Bxdc1_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Bxdc1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000008090
Gallus gallus
Domestic chicken
Ggal\RPF2
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    RPF2; ribosome production factor 2 homolog
    14 of 15
    618471
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Non3 - S6kII
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Non3 - Tsc1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Non3 - gig
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map

        3-62

        Cytogenetic map
        90F8-90F8
        Sequence location
        3R:18,223,722..18,224,942 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        90F8-90F8
        Limits computationally determined from genome sequence between P{EP}cpoEP3679 and P{EP}Dlc90FEP3634
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-62

        (FlyBase, 2016)

        3-61.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (5)
        Genomic Clones (18)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (27)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Andreyeva et al., 2019)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Non3 CG7993

        (Moutinho-Pereira et al., 2013)
        Source for database merge of
        Additional comments
        Other Comments

        dsRNA made from templates generated with primers directed against this gene results in a change in cell proliferation and cell size.

        (Guertin et al., 2006)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 28 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (3)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (38)