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Gene Dmel\MED25

General Information
Symbol	Dmel\MED25	Species	D. melanogaster
Name	Mediator complex subunit 25	Annotation symbol	CG12254
Feature type	protein_coding_gene	FlyBase ID	FBgn0038760
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	Arc92, dARC92
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	92C1-92C1	Sequence location	3R:15,834,669..15,837,434 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mediator complex subunit 25 is referred to in FlyBase by the symbol Dmel\MED25 (CG12254, FBgn0038760). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: RNA polymerase II transcription cofactor activity. There is experimental evidence that it is involved in the biological process: nervous system development; regulation of antimicrobial peptide biosynthetic process. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Mediator complex, subunit Med25, NR box; Mediator complex, subunit Med25, PTOV activation and synapsin 2; Mediator complex, subunit Med25, von Willebrand factor type A. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1624006_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of MED25. Gene sequence location is 3R:15834669..15837434.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 92C1-92C1   Limits computationally determined from genome sequence between P{PZ}l(3)1058510585 and P{EP}SyndEP409
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\MED25 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) MED25-RA FBtr0083825  NM_142597   2766   863
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein MED25-PA   FBpp0083234   96.6   863   8.65     NP_650854   AAF55729
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med25, PTOV activation and synapsin 2 (IPR021394) Mediator complex, subunit Med25, NR box (IPR021406) Mediator complex, subunit Med25, von Willebrand factor type A (IPR021419)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264534   AA441196   AA816561   AE014297 AAF55729   AW941651   AY069370 AAL39515   EC062761   GH946348   GH954480   GH957876   GH960560   GH961503   GH963903   GH965511   GH966312   GH967719   GH968135   GH969304   GH978110   GH978564   GH980212   GH980369   GH980496   GH982743
UniProtKB/Swiss-Prot	Q9VDR1
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    126.05 Larval Midgut    36.5 Larval Hindgut    64 Larval Malpighian Tubules    60.9 Larval Fat Body    54.5 Larval Salivary Gland    80.6 Larval Trachea    70.5 Larval Carcass    55.875 Adult Head    52 Adult Eye    68.1 Adult Brain    87 Adult Thoracic-Abdominal Ganglion    72.7 Adult Crop    53 Adult Midgut    54.4 Adult Hindgut    59 Adult Malpighian Tubules    38.2 Adult Fat Body    31.7 Adult Salivary Gland    62.6 Adult Heart    25.075 Adult VirginFemale Spermatheca    41 Adult InseminatedFemale Spermatheca    45.8 Adult Ovary    101.1 Adult Testis    19.8 Adult Male Accessory Gland    51.3 Adult Carcass    40.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    52   NA  Eye    68.1   NA  Brain    87   126.05  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    72.7   NA  Crop    53   36.5  Midgut    54.4   64  Hindgut    59   60.9  Malpighian Tubules    38.2   54.5  Fat Body    31.7   80.6  Salivary Gland    62.6   NA  Heart    25.075   70.5  Trachea    NA   NA  VirginFemale Spermatheca    41   NA  InseminatedFemale Spermatheca    45.8   NA  Ovary    101.1   NA  Testis    19.8   NA  Male Accessory Gland    51.3   55.875  Carcass    40.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    17 central nervous system, pupae P8    7 head, virgin 1-day female    4 head, virgin 4-day female    4 head, virgin 20-day female    2 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    3 digestive system, 20-day adult    3 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    10 carcass, 1-day adult    2 carcass, 4-day adult    3 carcass, 20-day adult    3 ovary, virgin 4-day female    11 ovary, mated 4-day female    14 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    17 embryo 04-06hr    19 embryo 06-08hr    20 embryo 08-10hr    13 embryo 10-12hr    13 embryo 12-14hr    9 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    6 embryo 20-22hr    4 embryo 22-24hr    3 larva L1    6 larva L2    5 larva L3 12hr old    4 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    7 pupae 3d postWPP    4 pupae 4d postWPP    3 adult male 01day    3 adult male 05day    4 adult male 30day    5 adult female 01day    9 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    13 Schneider line 2 Sg4    9 embryonic 1182-4H    9 embryonic GM2    10 embryonic Kc167    6 embryonic S1    9 embryonic S3    7 leg disc CME L1    10 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    14 wing disc ML-DmD21    10 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    6 ovary fGS/OSS    23 ovary OSC    16 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    7 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    8 Zinc 5 mM 12 hrs, larvae L3    8 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    7 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    10 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    6 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • LD07688 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038760 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12254-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of MED25 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of MED25 Class Mutagen Stocks Known lesion MED25GD7550 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED25HMJ02066 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED25HMS00256 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED25KK107516 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED25a.cKa in vitro construct - RNAi 0 Yes MED25cKa in vitro construct - RNAi 0 Yes MED25NIG.12254R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7550} NA P{KK107516} NA P{NIG.12254R} NA P{TRiP.HMJ02066} NA P{TRiP.HMS00256} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
nervous system development inferred from mutant phenotype (Koizumi et al., 2007) regulation of antimicrobial peptide biosynthetic process inferred from mutant phenotype (Valanne et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
RNA polymerase II transcription cofactor activity inferred from sequence or structural similarity (Boube et al., 2000)
Biological Process
CV term Evidence References
transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity (Boube et al., 2000)
Cellular Component
CV term Evidence References
mediator complex inferred from sequence or structural similarity (Boube et al., 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
MED25 - CG14480 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG15087 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG15625 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG17002 experimental knowledge based (Guruharsha et al., 2011) MED25 - CG30491 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG32479 experimental knowledge based (Guruharsha et al., 2011) MED25 - CG34159 experimental knowledge based (Guruharsha et al., 2011) MED25 - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG5114 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - CG7185 experimental knowledge based (Guruharsha et al., 2011) MED25 - MED14 experimental knowledge based (Guruharsha et al., 2011) MED25 - MED17 experimental knowledge based (Guruharsha et al., 2011) MED25 - MED23 experimental knowledge based (Guruharsha et al., 2011) MED25 - MED8 experimental knowledge based (Guruharsha et al., 2011) MED25 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - Rrp45 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED25 - dsh experimental knowledge based (Guruharsha et al., 2011) MED25 - ebi experimental knowledge based (Guruharsha et al., 2011) MED25 - fan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions MED25 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0038760 DroID - A comprehensive database of gene and protein interactions. • FBgn0038760 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42385
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NGH6F Dipteran inclusive ortholog search EOG62NJ7S Insect inclusive ortholog search EOG6SBCSX Arthropod inclusive ortholog search EOG65MKM9 Metazoa inclusive ortholog search EOG6QK4F1
Orthologs in Drosophila Species (EOG6NGH6F)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\MED25     Drosophila simulans   Dsim\GD19330     Drosophila sechellia   Dsec\GM17687 Y   Drosophila erecta   Dere\GG23647 Y   Drosophila yakuba   Dyak\GE25639 Y   Drosophila yakuba   Dyak\GE11214     Drosophila ananassae   Dana\GF16117 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26511 Y   Drosophila persimilis   Dper\GL23418 Y   Drosophila willistoni   Dwil\GK12965 Y   Drosophila virilis   Dvir\GJ10527 Y   Drosophila mojavensis   Dmoj\GI24180 Y   Drosophila grimshawi   Dgri\GH18669 Y
Orthologs in non-Drosophila Dipterans (EOG62NJ7S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017650   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017651
Orthologs in non-Dipteran Insects (EOG6SBCSX)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\MED25   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012144   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17011   Atta cephalotes Leafcutter ant  Acep\ACEP24219   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15072   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15073   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17146   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16489   Linepithema humile Argentine ant  Lhum\LH19195   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15718   Solenopsis invicta Red fire ant  Sinv\SINV16537   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008950   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003748   Bombyx mori Silkmoth  Bmor\BGIBMGA004245   Pediculus humanus Human body louse  Phum\PHUM261610   Tribolium castaneum Red flour beetle  Tcas\GLEAN_08353
Orthologs in non-Insect Arthropods (EOG65MKM9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW022843   Ixodes scapularis Deer tick  Isca\ISCW022845
Orthologs in non-Arthropod Metazoa (EOG6QK4F1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013259gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000969gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009167gn Y Danio rerio Zebrafish  Drer\med25 Y Xenopus tropicalis Western clawed frog  Xtro\med25 Y Mus musculus House mouse  Mmus\Ptov1 Y Mus musculus House mouse  Mmus\ENSMUSG00000002968 Y Rattus norvegicus Norway rat  Rnor\Ptov1 Y Rattus norvegicus Norway rat  Rnor\Med25 Y Homo sapiens Human  Hsap\ENSG00000104960 Y Homo sapiens Human  Hsap\MED25 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\ENSG00000104960     Hsap\MED25
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19328 Drosophila sechellia Dsec\GM17687 Drosophila erecta Dere\GG23647 Drosophila yakuba Dyak\GE25639 Drosophila ananassae Dana\GF16117 Drosophila pseudoobscura pseudoobscura Dpse\GA26511 Drosophila persimilis Dper\GL23418 Drosophila willistoni Dwil\GK12965 Drosophila virilis Dvir\GJ10527 Drosophila mojavensis Dmoj\GI24180 Drosophila grimshawi Dgri\GH18669
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	42501 y1 v1; P{TRiP.HMJ02066}attP40 33595 y1 sc* v1; P{TRiP.HMS00256}attP2
VDRC	v108249 P{KK107516}VIE-260B v47922 w1118; P{GD7550}v47922
Genomic Clones ( 1 )
	BACR10H14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 22 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD07688
Other clones	DPiM_LD07688
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD02101 LD15908
Other clones	58362 RP004048562 RP004113641 RP004217689 RP004222403 RP004240283 RP004241726 RP004242776 RP004259471 RP004263782 RP004443260 RP004459253 RP004463832 RP004481197 RP004491463 RP004511270 RP004534844 RP004544040
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0038760 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42385
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Arc92 CG12254 (Boube et al., 2000) Source for identity of: MED25 Arc92 (Bourbon et al., 2004)
Source for database merge of
Additional comments
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142597 RefSeq (Proteins) • NP_650854 DDBJ / EMBL / GenBank • AA264534 AAF55729 AAL39515 AW941651 AY069370 Entrez Gene - A searchable database of RefSeq genes. • 42385 UniProtKB/Swiss-Prot • Q9VDR1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med25, PTOV activation and synapsin 2 (IPR021394) Mediator complex, subunit Med25, NR box (IPR021406) Mediator complex, subunit Med25, von Willebrand factor type A (IPR021419) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD07688
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0038760 DroID - A comprehensive database of gene and protein interactions. • FBgn0038760 DRSC - Results from RNAi screens. • FBgn0038760 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0038760 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12254-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0038760 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42385 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42385 modMine - Data generated by the modENCODE project. • FBgn0038760
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	Arc92 (Foley and O'Farrell, 2004, Bourbon et al., 2004, ) CG12254 (Boutros et al., 2004, Keleman et al., 2009.8.5, Gim et al., 2001, Ni et al., 2010.12.1) dARC92 (Boube et al., 2000, Levine and Tjian, 2003) dDrip97 (Gim et al., 2001) LD07688 (Kamimura et al., 2001) Med25 (Kim et al., 2004, Kim and Lis, 2005) MED25 (Marr et al., 2006, Koizumi et al., 2007, Zhou et al., 2008, Valanne et al., 2010, Japanese National Institute of Genetics, 2012.5.21) med25 (Terriente-Félix et al., 2010)
Name Synonym	Mediator complex subunit 25 (Bourbon et al., 2004)
Secondary FlyBase IDs
References ( 26 )
Generate a list of	All references relating to this gene ( 26 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 4 ) Review  ( 2 ) Personal communication to FlyBase  ( 5 ) Letter  ( 1 ) Stock list  ( 1 ) Website  ( 1 )
Recent research papers ( 1 )
	Staller et al., 2013, Genetics 193(1): 51--61 Depleting Gene Activities in Early Drosophila Embryos with the "Maternal-Gal4-shRNA" System. [FBrf0220341] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...