FlyBase Gene Report: Dmel\Usp8

General Information

Symbol

Dmel\Usp8

Species

D. melanogaster

Name

Ubiquitin specific protease 8

Annotation Symbol

CG5798

Feature Type

protein_coding_gene

FlyBase ID

FBgn0038862

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Snapshot

Ubiquitin specific protease 8 is a protease involved in protein deubiquitination. It contributes to the integrity of ESCRT sorting machinery and the regulation of Hedgehog and Wingless signaling pathways. [Date last reviewed: 2016-06-09]

Other Summaries

Auto summary
Gene Group
Interactive Fly

Also Known As

Ubpy

Key Links

Genomic Location

Cytogenetic map

93C1-93C1

Sequence location

3R:21,134,262..21,138,214 [+]

Recombination map

3-71

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI Genome Data Viewer

UCSC Genome Browser

Ensembl Genome Browser

PopFly Genome Browser

GO Summary Ribbons

[Detailed GO annotations]

Families, Domains and Molecular Function

Gene Group Membership (FlyBase)

UBIQUITIN SPECIFIC PROTEASES (DEUBIQUITINASES)

Positive Regulators of Wnt-TCF Signaling Pathway

Positive Regulators of Hedgehog Signaling Pathway

Protein Family (UniProt, Sequence Similarities)

Protein Domains/Motifs

InterPro

Peptidase C19, ubiquitin carboxyl-terminal hydrolase; Rhodanese-like domain; USP8 dimerisation domain; Ubiquitin specific protease, conserved site; Ubiquitin specific protease domain; Rhodanese-like domain superfamily; Papain-like cysteine peptidase superfamily

Molecular Function (see GO section for details)

Experimental Evidence

thiol-dependent ubiquitin-specific protease activity

Predictions / Assertions

Summaries

Gene Group Membership

UBIQUITIN SPECIFIC PROTEASES (DEUBIQUITINASES) -: The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. (Adapted from PMID:19626045).
Positive Regulators of Wnt-TCF Signaling Pathway -: Positive regulators of canonical Wnt signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Usp8 or the JBrowse view of Dmel\Usp8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Usp8-RA

FBtr0084016

NM_142691

3204

896

Usp8-RB

FBtr0304714

NM_001260298

1546

367

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Usp8-PA

FBpp0083419

102.7

896

7.31

NP_650948

AAF55858

Usp8-PB

FBpp0293257

42.0

367

8.91

NP_001247227

AFH06545

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Usp8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

thiol-dependent ubiquitin-specific protease activity

inferred from direct assay

(Xia et al., 2012)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

imaginal disc-derived wing margin morphogenesis

inferred from mutant phenotype

(Mukai et al., 2010)

neuron remodeling

inferred from mutant phenotype

(Loncle et al., 2015)

positive regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Mukai et al., 2010)

positive regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444

(Xia et al., 2012)

protein deubiquitination

inferred from mutant phenotype

(Xia et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

ubiquitin-dependent protein catabolic process

inferred from electronic annotation with InterPro:IPR018200

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

cytosol

inferred from direct assay

(Xia et al., 2012)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

cytosol

inferred from direct assay

(Xia et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Usp8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Usp8

View exonic expression by tissue for Dmel\Usp8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0038862

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5798-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5798

Flygut - An atlas of the Drosophila adult midgut

FBgn0038862

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs and Phenotypes

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Allele of Usp8

Class

Mutagen

Associated Insertion

Stocks

Known lesion

Usp8^KO

amorphic allele - molecular evidence

Yes

Usp8^5-SZ-3473

P{RS5}Usp8^5-SZ-3473

Yes

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Usp8

Allele of Usp8

Mutagen

Associated Transgenic Construct

Stocks

Usp8^{CS.UAS.Tag:FLAG}

PBac{UAS-Flag-USP8.CS}

Usp8^{NT3.CS.UAS.Tag:HA}

PBac{UAS-HA-USP8.NT3.CS}

Usp8^{NT3.UAS.Tag:HA}

PBac{UAS-HA-USP8.NT3}

Usp8^{-SIM.UAS.cMa.Tag:FLAG}

P{UAS-Usp8.-SIM.FLAG}

Usp8^UAS.cLa

in vitro construct - regulatory fusion

P{UAS-Usp8.wt}

Usp8^{UAS.cMa.Tag:FLAG}

P{UAS-Usp8.FLAG}

Usp8^UAS.cMa

in vitro construct - regulatory fusion

P{UAS-Usp8.M}

Transgenic constructs containing regulatory region of Usp8

Deletions and Duplications ( 0 )

Summary of Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^tim.PE

lethal | larval stage, with Scer\GAL4^elav.PU

Usp8^HMS01898

lethal | pupal stage, with Scer\GAL4^elav.PU

Usp8^HMS01898

lethal - all die before end of embryonic stage

Usp8^KO

lethal - all die before end of larval stage, with Scer\GAL4^sqh.PW

Usp8^HMS01898

lethal - all die before end of pupal stage, with Scer\GAL4^dpp.PU

Usp8^KK100733

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Usp8^GD1285

lethal - all die before end of pupal stage | somatic clone

Usp8^KO

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Usp8^GD1285

Other Phenotypes

Allele

locomotor rhythm defective, with Scer\GAL4^Dvir\Pdf.1.9

locomotor rhythm defective, with Scer\GAL4^P2.4.Pdf

locomotor rhythm defective, with Scer\GAL4^tim.PE, Scer\GAL80^ts.αTub84B

neuroanatomy defective | pupal stage, with Scer\GAL4^ppk.1.9

short lived, with Scer\GAL4^Mef2.PR

Usp8^GD1285

some die during embryonic stage

Usp8^KO

some die during larval stage, with Scer\GAL4^sqh.PW

Usp8^HMS01898

some die during pupal stage

Usp8^KO

some die during pupal stage, with Scer\GAL4^dpp.PU

Usp8^KK100733

some die during pupal stage, with Scer\GAL4^pnr-MD237

Usp8^GD1285

visible, with Scer\GAL4^bbg-C96

Usp8^KK100733

visible, with Scer\GAL4^Bx-MS1096

visible, with Scer\GAL4^sd.PU

visible (with Usp8^KO)

Usp8^UAS.cMa

visible (with Usp8^UAS.cMa)

Usp8^KO

visible | adult stage, with Scer\GAL4^Bx-MS1096

Usp8^HMS01898

visible | adult stage, with Scer\GAL4^GMR.PF

Usp8^KK100733

Phenotype manifest in

Allele

dendrite | pupal stage, with Scer\GAL4^ppk.1.9

dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.1.9

eye | adult stage, with Scer\GAL4^GMR.PF

Usp8^KK100733

wing, with Scer\GAL4^bbg-C96

Usp8^KK100733

wing, with Scer\GAL4^Bx-MS1096

wing blade, with Scer\GAL4^Bx-MS1096

Usp8^HMS01898

wing margin bristle, with Scer\GAL4^bbg-C96

Usp8^KK100733

wing margin bristle, with Scer\GAL4^sd.PU

wing margin bristle (with Usp8^KO)

Usp8^UAS.cMa

wing margin bristle (with Usp8^UAS.cMa)

Usp8^KO

wing vein L3, with Scer\GAL4^Bx-MS1096

Usp8^UAS.Tag:FLAG

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\USP8

14 of 15

Yes

4 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\USP27X

1 of 15

Hsap\USP29

1 of 15

Yes

Hsap\USP37

1 of 15

Yes

Hsap\USP43

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Usp8

14 of 15

Yes

Mmus\Usp2

4 of 15

Mmus\Usp50

3 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Usp27x

1 of 15

Mmus\Usp29

1 of 15

Yes

Mmus\Usp32

1 of 15

Mmus\Usp37

1 of 15

Yes

Mmus\Usp43

1 of 15

Rattus norvegicus (Norway rat) (11)

Rnor\Usp8

11 of 13

Yes

Rnor\Usp2

3 of 13

Rnor\Usp50

3 of 13

Yes

Rnor\Usp21

2 of 13

Rnor\Usp4

1 of 13

Yes

Rnor\Usp19

1 of 13

Rnor\Usp26

1 of 13

Yes

Rnor\Usp29

1 of 13

Yes

Rnor\Usp32

1 of 13

Rnor\Usp37

1 of 13

Yes

Rnor\Usp43

1 of 13

Xenopus tropicalis (Western clawed frog) (5)

Xtro\usp8

8 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\usp8

10 of 15

Yes

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\usp-50

9 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Cele\F07C6.3

1 of 15

Yes

Cele\usp-33

1 of 15

Arabidopsis thaliana (thale-cress) (5)

Atha\UBP5

2 of 9

Yes

Atha\UBP8

2 of 9

Yes

Atha\UBP9

2 of 9

Yes

Atha\UBP10

2 of 9

Yes

Atha\UBP11

2 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\UBP5

7 of 15

Yes

6 of 15

3 of 15

2 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\ubp4

4 of 12

Yes

Spom\ubp1

3 of 12

Spom\ubp12

3 of 12

Spom\ubp8

1 of 12

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919021D )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Usp8

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19137

Drosophila persimilis

Dper\GL12603

Drosophila willistoni

Dwil\GK12180

Drosophila virilis

Dvir\GJ23355

Drosophila mojavensis

Dmoj\GI10137

Drosophila grimshawi

Dgri\GH18183

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150346 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17016491

Glossina morsitans

Tsetse fly

Gmor\GMOY000704

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04616

Mayetiola destructor

Hessian fly

Mdes\Mdes010327

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012219

Anopheles darlingi

American malaria mosquito

Adar\ADAC007510

Anopheles gambiae

Malaria mosquito

Agam\AGAP002900

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002564

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W021D )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008661

Bombyx mori

Silkmoth

Bmor\BGIBMGA008660

Danaus plexippus

Monarch butterfly

Dple\DPOGS215177-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS215176-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL004423

Heliconius melpomene

Postman butterfly

Hmel\HMEL009253

Apis florea

Little honeybee

Aflo\3813683588

Apis mellifera

Western honey bee

Amel\GB46344

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP23645

Bombus terrestris

Buff-tailed bumblebee

Bter\15205825

Linepithema humile

Argentine ant

Lhum\LH13927

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001486

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG025741

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG015629

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG034350

Pediculus humanus

Human body louse

Phum\PHUM002680

Rhodnius prolixus

Kissing bug

Rpro\RPRC014066

Cimex lectularius

Bed bug

Clec\CLEC010975

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI21478

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02070

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01YW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012767

Ixodes scapularis

Black-legged tick

Isca\ISCW005312

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72428.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM72427.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g00980

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_7618

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_266245

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_66136

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0120 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_010340gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Usp8

Ciona intestinalis

Vase tunicate

Cint\LOC100186834

Ciona intestinalis

Vase tunicate

Cint\LOC100186923

Gallus gallus

Domestic chicken

Ggal\USP8

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000006755

Human Disease Model Data

FlyBase Human Disease Model Reports

Alleles Reported to Model Human Disease (Disease Ontology)

Download

Models ( 0 )

Allele

Disease

Evidence

References

Interactions ( 2 )

Allele

Disease

Interaction

References

Usp8^KK100733

exacerbates neurodegenerative disease

modeled by Hsap\TARDBP^WT.UAS.GFP

(Hans et al., 2014)

Usp8^HMS01898

ameliorates synucleinopathy

modeled by Hsap\SNCA^UAS.cFa

(Alexopoulou et al., 2016)

modeled by Hsap\SNCA^A53T.UAS

(Alexopoulou et al., 2016)

Comments ( 0 )

Human Orthologs (via DIOPT v7.1)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

Complementation?

Transgene?

USP8; ubiquitin specific peptidase 8

14 of 15

603158

PITUITARY ADENOMA 4, ACTH-SECRETING; PITA4

USP2; ubiquitin specific peptidase 2

4 of 15

604725

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please look at the Interaction Group reports for full details of the physical interactions

protein-protein

Interacting group

Assay

References

Usp8 - Clk

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Luo et al., 2012)

Usp8 - Hrs

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Zhang et al., 2014)

Usp8 - smo

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Ma et al., 2016, Fan et al., 2013, Li et al., 2012, Xia et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Sinenko, 2017)

enhanceable

(Xia et al., 2012)

suppressible

(Sinenko, 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67480

DroID - A comprehensive database of gene and protein interactions.

FBgn0038862

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

42509

Pathways

Gene Group - Pathway Membership (FlyBase)

Positive Regulators of Wnt-TCF Signaling Pathway -: Positive regulators of canonical Wnt signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling down-regulation the pathway, enhancing the activation of transcription of hh-responsive genes.

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-71

Cytogenetic map

93C1-93C1

Sequence location

3R:21,134,262..21,138,214 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93C1-93C1

Limits computationally determined from genome sequence between P{PZ}Atpα⁰¹¹⁶⁴ and P{PZ}mod(mdg4)⁰⁷⁰³⁸&P{lacW}mod(mdg4)^L3101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-71

(FlyBase, 2016)

3-73.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

38982

y¹ v¹; P{TRiP.HMS01898}attP40

39022

y¹ sc^* v¹; P{TRiP.HMS01941}attP2/TM3, Sb¹

Kyoto

126082

w¹¹¹⁸; P{RS5}Usp8^5-SZ-3473

VDRC

v330131

P{VSH330131}attP40

v107623

P{KK100733}VIE-260B

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (48)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0038862

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0038862

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42509

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Xia et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ubpy CG5798

(Mukai et al., 2010)

Source for identity of: Usp8 Ubpy

(FlyBase, 2009-)

Source for database merge of

Additional comments

Changed symbol from 'Ubpy' to 'Usp8' because: i) The original symbol (from FBrf0211204) was based on the symbol of the mammalian/human ortholog, and this symbol is currently 'USP8'; ii) all other 'Usp' genes in D. melanogaster named for their molecular function/orthology have a 'Usp' prefix and reflect the current HGNC nomenclature of their human orthologs; iii) several key publications refer to this gene as 'Usp8' (FBrf0211204, FBrf0218247, FBrf0219991, FBrf0225968).

(FlyBase, 2009-)

Other Comments

Usp8 is required cell autonomously for dendrite pruning in sensory neurons.

(Loncle et al., 2015)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; increased expression in per⁰¹, tim⁰¹ and decreased expression in Clk^Jrk background.

(Claridge-Chang et al., 2001)

Identified as one of 10 highest fold cycling genes as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

(McDonald and Rosbash, 2001)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42509

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

InterPro - A database of protein families, domains and functional sites

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

67480

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones

FBgn0038862

DroID - A comprehensive database of gene and protein interactions.

FBgn0038862

DRSC - Results frm RNAi screens

FBgn0038862

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0038862

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5798-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5798

Flygut - An atlas of the Drosophila adult midgut

FBgn0038862

FlyMine - An integrated database for Drosophila genomics

FBgn0038862

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

42509

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

42509

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5798

modMine - A data warehouse for the modENCODE project

FBgn0038862

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG5798

(Meadows, 2017.1.11, Aradska et al., 2015, Jacomin et al., 2015, Zhou et al., 2015, Engel et al., 2014, Yang et al., 2014, Tsou et al., 2012, Zhang et al., 2012, Napoletano et al., 2011, Kula-Eversole et al., 2010, Mukai et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Zhang et al., 2008, Takahashi et al., 2007, Wijnen et al., 2006, Cirelli et al., 2005)

CT18196

(Ceriani et al., 2002)

Q9VDD8

(Clague et al., 2013)

UBPY

(Lee et al., 2016, Ma et al., 2016, Hans et al., 2014, Chen and Jiang, 2013, Li et al., 2012)

UBPY/USP8

(Li et al., 2012)

USP8

(Abruzzi et al., 2017, Tataroglu and Emery, 2015, Chen and Jiang, 2013, Fan et al., 2013, Luo et al., 2012, Xia et al., 2012)

UbpY

(Engel et al., 2014)

Ubpy

(Sinenko, 2017, Jacomin et al., 2015, Yang et al., 2014, Tsou et al., 2012)

Usp8

(Alexopoulou et al., 2016, Zhou et al., 2015, Engel et al., 2014)

dUBPY

(Mukai et al., 2010)

ubpy

(Loncle et al., 2015, Pradhan-Sundd and Verheyen, 2015)

usp8

(Ristic et al., 2016, Luo et al., 2012)

Name Synonyms

UBPY ortholog

Ubiquitin specific protease 8

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (64)

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