FB2021_05, released October 15, 2021

Gene: Dmel\Usp8

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General Information
Symbol
Dmel\Usp8
Species
D. melanogaster
Name
Ubiquitin specific protease 8
Annotation Symbol
CG5798
Feature Type
protein_coding_gene
FlyBase ID
FBgn0038862
Gene Model Status
Current
Stock Availability
5 publicly available
Enzyme Name (EC)
Ubiquitinyl hydrolase 1 (3.4.19.12)
Gene Summary
Ubiquitin specific protease 8 (Usp8) encodes a protease involved in protein deubiquitination. It contributes to the integrity of ESCRT sorting machinery and the regulation of Hedgehog and Wingless signaling pathways. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Ubpy

Key Links
Genomic Location
Cytogenetic map
93C1-93C1
Sequence location
Recombination map
3-71
RefSeq locus
NT_033777 REGION:21134262..21138214
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables thiol-dependent deubiquitinase
inferred from direct assay
(Xia et al., 2012)
inferred from mutant phenotype
(Zhou et al., 2018)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (7 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in imaginal disc-derived wing margin morphogenesis
inferred from mutant phenotype
(Mukai et al., 2010)
involved_in neuron remodeling
inferred from mutant phenotype
(Loncle et al., 2015)
involved_in positive regulation of canonical Wnt signaling pathway
inferred from mutant phenotype
(Mukai et al., 2010)
involved_in positive regulation of receptor recycling
inferred from mutant phenotype
(Mukai et al., 2010)
involved_in positive regulation of smoothened signaling pathway
inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444
(Ma et al., 2016, Xia et al., 2012)
inferred from genetic interaction with FLYBASE:Uch-L5; FB:FBgn0011327
(Zhou et al., 2018)
involved_in protein deubiquitination
inferred from mutant phenotype
(Zhou et al., 2018, Xia et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in ubiquitin-dependent protein catabolic process
inferred from electronic annotation with InterPro:IPR018200
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Xia et al., 2012)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal) (3.4.19.12)
Predictions / Assertions
-
Summaries
Gene Snapshot
Ubiquitin specific protease 8 (Usp8) encodes a protease involved in protein deubiquitination. It contributes to the integrity of ESCRT sorting machinery and the regulation of Hedgehog and Wingless signaling pathways. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
USP DEUBIQUITINASES -
The Ubiquitin Specific Proteases (USP) is the largest sub-family of deubiquitinases. They are cysteine proteases and catalyze the removal of ubiquitin from ubiquitin chains and ubiquitinated proteins. USP deubiquitinases belong to MEROPS family C19. (Adapted from PMID:19626045).
Pathway (FlyBase)
Positive Regulators of Wnt-TCF Signaling Pathway -
Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
Positive Regulators of Hedgehog Signaling Pathway -
Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Usp8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Usp8-RA
FBtr0084016
NM_142691
3204
896
Usp8-RB
FBtr0304714
NM_001260298
1546
367
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Usp8-PA
FBpp0083419
102.7
896
7.31
NP_650948
AAF55858
Usp8-PB
FBpp0293257
42.0
367
8.91
NP_001247227
AFH06545
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Usp8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
eye
(Xu et al., 2004)
Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from direct assay
(Xia et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Usp8 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Usp8
Transgenic constructs containing regulatory region of Usp8
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4tim.PE
lethal | larval stage, with Scer\GAL4elav.PU
lethal | pupal stage, with Scer\GAL4elav.PU
lethal - all die before end of larval stage, with Scer\GAL4sqh.PW
lethal - all die before end of pupal stage, with Scer\GAL4dpp.PU
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
lethal - all die before end of pupal stage | somatic clone
lethal - all die during embryonic stage
partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
Other Phenotypes
Allele
abnormal locomotor rhythm, with Scer\GAL4Dvir\Pdf.1.9
abnormal locomotor rhythm, with Scer\GAL4P2.4.Pdf
abnormal locomotor rhythm, with Scer\GAL4tim.PE, Scer\GAL80ts.αTub84B
abnormal neuroanatomy | pupal stage, with Scer\GAL4ppk.1.9
short lived, with Scer\GAL4Mef2.PR
some die during embryonic stage
some die during larval stage, with Scer\GAL4sqh.PW
some die during pupal stage, with Scer\GAL4dpp.PU
some die during pupal stage, with Scer\GAL4pnr-MD237
some die during pupal stage | somatic clone
visible, with Scer\GAL4bbg-C96
visible, with Scer\GAL4Bx-MS1096
visible, with Scer\GAL4sd.PU
visible (with Usp8KO)
visible (with Usp8UAS.cMa)
visible | adult stage, with Scer\GAL4Bx-MS1096
visible | adult stage, with Scer\GAL4GMR.PF
Phenotype manifest in
Allele
dendrite | pupal stage, with Scer\GAL4ppk.1.9
eye | adult stage, with Scer\GAL4GMR.PF
larval dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4ppk.1.9
wing, with Scer\GAL4bbg-C96
wing, with Scer\GAL4Bx-MS1096
wing blade, with Scer\GAL4Bx-MS1096
wing margin bristle, with Scer\GAL4bbg-C96
wing margin bristle, with Scer\GAL4sd.PU
wing margin bristle (with Usp8KO)
wing margin bristle (with Usp8UAS.cMa)
wing vein L3, with Scer\GAL4Bx-MS1096
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (33)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\USP8
13 of 15
Yes
Yes
1  
Hsap\USP2
5 of 15
No
No
Hsap\USP50
3 of 15
No
Yes
Hsap\USP3
2 of 15
No
No
Hsap\USP4
2 of 15
No
Yes
Hsap\USP11
2 of 15
No
No
Hsap\USP21
2 of 15
No
No
Hsap\USP15
1 of 15
No
No
Hsap\USP17L1
1 of 15
No
No
Hsap\USP17L2
1 of 15
No
No
Hsap\USP17L3
1 of 15
No
No
Hsap\USP17L5
1 of 15
No
No
Hsap\USP17L7
1 of 15
No
No
Hsap\USP17L8
1 of 15
No
No
Hsap\USP17L9P
1 of 15
No
Yes
Hsap\USP17L10
1 of 15
No
No
Hsap\USP17L11
1 of 15
No
No
Hsap\USP17L12
1 of 15
No
No
Hsap\USP17L13
1 of 15
No
No
Hsap\USP17L15
1 of 15
No
No
Hsap\USP17L17
1 of 15
No
No
Hsap\USP17L18
1 of 15
No
No
Hsap\USP17L19
1 of 15
No
No
Hsap\USP17L20
1 of 15
No
No
Hsap\USP17L21
1 of 15
No
No
Hsap\USP17L22
1 of 15
No
No
Hsap\USP22
1 of 15
No
No
Hsap\USP26
1 of 15
No
Yes
Hsap\USP27X
1 of 15
No
No
Hsap\USP29
1 of 15
No
Yes
Hsap\USP32
1 of 15
No
No
2  
Hsap\USP37
1 of 15
No
Yes
Hsap\USP43
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (20)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Usp8
13 of 15
Yes
Yes
Mmus\Usp2
5 of 15
No
No
Mmus\Usp50
3 of 15
No
Yes
Mmus\Usp3
2 of 15
No
No
Mmus\Usp4
2 of 15
No
Yes
Mmus\Usp11
2 of 15
No
No
Mmus\Usp15
2 of 15
No
Yes
Mmus\Usp17la
1 of 15
No
No
Mmus\Usp17lb
1 of 15
No
No
Mmus\Usp17lc
1 of 15
No
No
Mmus\Usp17ld
1 of 15
No
No
Mmus\Usp17le
1 of 15
No
No
Mmus\Usp21
1 of 15
No
No
Mmus\Usp22
1 of 15
No
No
Mmus\Usp26
1 of 15
No
Yes
Mmus\Usp27x
1 of 15
No
No
Mmus\Usp29
1 of 15
No
Yes
Mmus\Usp32
1 of 15
No
No
Mmus\Usp37
1 of 15
No
Yes
Mmus\Usp43
1 of 15
No
No
Rattus norvegicus (Norway rat) (14)
Rnor\Usp8
11 of 13
Yes
Yes
Rnor\Usp2
4 of 13
No
No
Rnor\Usp50
3 of 13
No
Yes
Rnor\Usp21
2 of 13
No
No
Rnor\LOC689730
1 of 13
No
No
Rnor\LOC100910107
1 of 13
No
No
Rnor\Usp3
1 of 13
No
No
Rnor\Usp4
1 of 13
No
Yes
Rnor\Usp11
1 of 13
No
No
Rnor\Usp15
1 of 13
No
No
Rnor\Usp26
1 of 13
No
Yes
Rnor\Usp29
1 of 13
No
Yes
Rnor\Usp37
1 of 13
No
Yes
Rnor\Usp43
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (7)
Xtro\usp8
7 of 12
Yes
Yes
Xtro\usp2
3 of 12
No
No
Xtro\usp15
2 of 12
No
Yes
Xtro\usp21
2 of 12
No
No
Xtro\usp3
1 of 12
No
No
Xtro\usp4
1 of 12
No
No
Xtro\usp37
1 of 12
No
Yes
Danio rerio (Zebrafish) (11)
Drer\usp8
11 of 15
Yes
Yes
Drer\usp2a
5 of 15
No
No
Drer\usp2b
4 of 15
No
No
Drer\usp4
2 of 15
No
Yes
Drer\usp11
2 of 15
No
Yes
Drer\usp21
2 of 15
No
No
Drer\LOC100535263
1 of 15
No
Yes
Drer\usp3
1 of 15
No
No
Drer\usp15
1 of 15
No
Yes
Drer\usp32
1 of 15
No
No
Drer\usp43b
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\usp-50
11 of 15
Yes
Yes
Cele\usp-3
3 of 15
No
No
Cele\F07A11.4
2 of 15
No
Yes
Cele\H34C03.2
2 of 15
No
Yes
Cele\C04E6.5
1 of 15
No
Yes
Cele\cyk-3
1 of 15
No
No
Cele\T22F3.2
1 of 15
No
Yes
Cele\usp-33
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (11)
Atha\UBP19
2 of 9
Yes
No
Atha\UBP5
1 of 9
No
No
Atha\UBP8
1 of 9
No
No
Atha\UBP15
1 of 9
No
No
Atha\UBP16
1 of 9
No
No
Atha\UBP17
1 of 9
No
No
Atha\UBP18
1 of 9
No
No
Atha\UBP20
1 of 9
No
Yes
Atha\UBP21
1 of 9
No
Yes
Atha\UBP23
1 of 9
No
No
Atha\UBP25
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\UBP5
7 of 15
Yes
Yes
Scer\DOA4
6 of 15
No
Yes
1  
Scer\UBP8
2 of 15
No
No
Scer\UBP12
2 of 15
No
No
Scer\UBP7
1 of 15
No
No
Scer\UBP11
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (5)
Spom\ubp4
5 of 12
Yes
Yes
Spom\ubp1
2 of 12
No
No
Spom\ubp8
2 of 12
No
No
Spom\ubp12
2 of 12
No
No
Spom\ubp16
1 of 12
No
No
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919021D )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00009055
Drosophila simulans
Dsim\GD19401
Drosophila sechellia
Dsec\GM15069
Drosophila erecta
Dere\GG24410
Drosophila yakuba
Dyak\GE25711
Drosophila ananassae
Dana\GF23156
Drosophila pseudoobscura pseudoobscura
Dpse\GA19137
Drosophila persimilis
Dper\GL12603
Drosophila willistoni
Dwil\GK12180
Drosophila virilis
Dvir\GJ23355
Drosophila mojavensis
Dmoj\GI10137
Drosophila grimshawi
Dgri\GH18183
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150346 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17016491
Glossina morsitans
Tsetse fly
Gmor\GMOY000704
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_04616
Mayetiola destructor
Hessian fly
Mdes\Mdes010327
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012219
Anopheles darlingi
American malaria mosquito
Adar\ADAC007510
Anopheles gambiae
Malaria mosquito
Agam\AGAP002900
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ002564
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W021D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA008661
Bombyx mori
Silkmoth
Bmor\BGIBMGA008660
Danaus plexippus
Monarch butterfly
Dple\DPOGS215177-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS215176-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL004423
Heliconius melpomene
Postman butterfly
Hmel\HMEL009253
Apis florea
Little honeybee
Aflo\3813683588
Apis mellifera
Western honey bee
Amel\GB46344
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP23645
Bombus terrestris
Buff-tailed bumblebee
Bter\15205825
Linepithema humile
Argentine ant
Lhum\LH13927
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001486
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG025741
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG015629
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG034350
Pediculus humanus
Human body louse
Phum\PHUM002680
Rhodnius prolixus
Kissing bug
Rpro\RPRC014066
Cimex lectularius
Bed bug
Clec\CLEC010975
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI21478
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_02070
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01YW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR012767
Ixodes scapularis
Black-legged tick
Isca\ISCW005312
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72428.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72427.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g00980
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_7618
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_266245
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_66136
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0120 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_010340gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Usp8
Ciona intestinalis
Vase tunicate
Cint\LOC100186834
Ciona intestinalis
Vase tunicate
Cint\LOC100186923
Gallus gallus
Domestic chicken
Ggal\USP8
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000006755
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (17)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Usp2
6 of 10
not
3 of 10
Usp15-31
2 of 10
Usp32
2 of 10
DUBAI
1 of 10
faf
1 of 10
scny
1 of 10
Usp1
1 of 10
Usp5
1 of 10
Usp7
1 of 10
Usp10
1 of 10
Usp14
1 of 10
Usp16-45
1 of 10
Usp20-33
1 of 10
Usp30
1 of 10
Usp39
1 of 10
Usp47
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    USP8; ubiquitin specific peptidase 8
    model of  ACTH-secreting pituitary adenoma
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 4 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    USP8; ubiquitin specific peptidase 8
    13 of 15
    603158
     
    USP2; ubiquitin specific peptidase 2
    5 of 15
    604725
        USP50; ubiquitin specific peptidase 50
        3 of 15
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Usp8 - Clk
            anti tag coimmunoprecipitation, western blot, anti tag western blot
            (Luo et al., 2012)
            Usp8 - Hrs
            anti tag coimmunoprecipitation, western blot, anti tag western blot
            (Zhang et al., 2014)
            Usp8 - Rack1
            anti tag coimmunoprecipitation, anti tag western blot
            (Li et al., 2020)
            Usp8 - smo
            anti tag coimmunoprecipitation, anti tag western blot, pull down
            (Li et al., 2020, Ma et al., 2016, Fan et al., 2013, Li et al., 2012, Xia et al., 2012)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Dronc
            enhanceable
            Usp8
            (Sinenko, 2017)
            ptc
            enhanceable
            Usp8
            (Xia et al., 2012)
            rpr
            suppressible
            Dark|Usp8
            (Sinenko, 2017)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Positive Regulators of Wnt-TCF Signaling Pathway -
            Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).
            Positive Regulators of Hedgehog Signaling Pathway -
            Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
            Metabolic Pathways
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            External Data
            Linkouts
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-71
            Cytogenetic map
            93C1-93C1
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            93C1-93C1
            Limits computationally determined from genome sequence between P{PZ}Atpα01164 and P{PZ}mod(mdg4)07038&P{lacW}mod(mdg4)L3101
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-71

            (FlyBase, 2016)

            3-73.7

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (5)
            Genomic Clones (20)
            cDNA Clones (48)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Xia et al., 2012)
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: Ubpy CG5798

            (Mukai et al., 2010)

            Source for identity of: Usp8 Ubpy

            (FlyBase, 2009-)
            Source for database merge of
            Additional comments

            Changed symbol from 'Ubpy' to 'Usp8' because: i) The original symbol (from FBrf0211204) was based on the symbol of the mammalian/human ortholog, and this symbol is currently 'USP8'; ii) all other 'Usp' genes in D. melanogaster named for their molecular function/orthology have a 'Usp' prefix and reflect the current HGNC nomenclature of their human orthologs; iii) several key publications refer to this gene as 'Usp8' (FBrf0211204, FBrf0218247, FBrf0219991, FBrf0225968).

            (FlyBase, 2009-)
            Other Comments

            Usp8 is required cell autonomously for dendrite pruning in sensory neurons.

            (Loncle et al., 2015)

            Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; increased expression in per01, tim01 and decreased expression in ClkJrk background.

            (Claridge-Chang et al., 2001)

            Identified as one of 10 highest fold cycling genes as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day.

            (McDonald and Rosbash, 2001)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 45 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (14)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            References (77)