minc, gfl, gleeful, myoblasts incompetent
zinc finger transcription factor - has an essential regulatory role in the specification and function of fusion-competent myoblasts
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Gene model reviewed during 5.48
2 (northern blot)
None of the polypeptides share 100% sequence identity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lmd using the Feature Mapper tool.
Comment: reported as muscle system primordium
Comment: reported as muscle system primordium
lmd expression is first observed in the embryo at late stage 11 in repeateing patches of visceral mesoderm. Prominent expression is observed in somatic and visceral mesodermal cells throughout stage 12. By stage 13, low levels are seen in repeating groups of mesodermal cells and visceral mesoderm expression has subsided. lmd expression is gone from somatic mesodermal cells before fusion and is never observed in muscle fibers or in heart progenitors.
Expression is observed in mesodermal tissues starting in late embryonic stages.
Expression of lmd protein is observed in two bands in the fusion-competent myoblasts of the somatic and visceral mesoderm, a dorsal band corresponding to the fusion-competent myoblasts of the visceral mesoderm and a ventral band corresponding to the fusion-competent myoblasts of the somatic mesoderm.
lmd expression is first observed in the embryo at late stage 11 in repeateing patches of visceral mesoderm. Prominent expression is observed in somatic and visceral mesodermal cells throughout stage 12. By stage 13, low levels are seen in repeating groups of mesodermal cells and visceral mesoderm expression has subsided. lmd expression is gone from somatic mesodermal cells before fusion and is never observed in muscle fibers or in heart progenitors. Co-staining with Ecol\lacZkirre-rP298 and Mef2 markers shows that lmd protein is expressed at high levels in fusion-competent myoblasts and is barely detectable or undetectable in founder cells.
GBrowse - Visual display of RNA-Seq signals
View Dmel\lmd in GBrowse 23-77
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal antibody
Source for identity of: Lmd gfl
Source for identity of: Lmd CG4677
Source for merge of: CG4677 anon- EST:CL2d4
Source for identity of Lmd CG4677 was sequence comparison ( date:010702 ).
ChEST reveals this is a target of Mef2.
lmd is required for fusion-competent myoblasts to differentiate.
Fusion-competent myoblasts remain as an immature population of mesodermal cells that cannot contribute to muscle formation in mutant embryos, resulting in a failure to form multinucleate muscles.
lmd mutant embryos show loss of expression of two key differentiation and fusion genes, Mef2 and sns, in fusion-competent myoblasts and completely lack multinucleate somatic muscle fibres. However, founder cells are specified and retain their capacity to differentiate into mononucleate muscle cells.
lmd is essential for somatic muscle development.
Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.
The gene is named "myoblasts incompetent" after the mutant phenotype; mutants show a defect in the differentiation of fusion-competent myoblasts (while there is no apparent requirement for the gene in the differentiation of founder myoblasts).