Nup98, Nup96, l(3)95BCd, Nucleoporin 98
The larger Nup98-RA transcript encodes a single, large precursor polyprotein that, in other eukaryotes, and thus presumably in flies, autoproteolytically cleaves itself between residues Phe1028 and Ser1029, yielding separate Nup98 and Nup96 proteins (FBrf0159638).
Gene model reviewed during 5.41
Gene model reviewed during 5.47
Part of the nuclear pore complex (NPC) (PubMed:25310983). Interacts with Rae1 (PubMed:21874015, PubMed:28554770). Nuclear pore complex protein Nup98: Interacts with pzg and Chro (PubMed:25310983). Interacts with MBD-R2; the interaction allows Nup98 recruitment to chromatin (PubMed:25310983). Interacts with Trx (PubMed:25310983). Interacts with Wds (PubMed:25310983). Interacts with Mtor and Cp190 (PubMed:28366641). Upon ecdysone stimulation, interacts with EcR, CTCF, su(Hw) and Trl (PubMed:28366641).
Isoform A and isoform C are autoproteolytically cleaved to yield Nup98 and Nup96 or Nup98 only, respectively.
Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup98-96 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Nup98-96 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.
This gene is alternatively transcribed, giving rise to a minor 3.5kb Nup98 transcript and a major 7.3kb Nup98-Nup96 transcript. Which may autoproteolytically cleaves itself yielding separate Nup98 and Nup96 proteins.