Ime4, Inducer of meiosis 4
methyltransferase- internal modification of mRNA - regulates alternative splicing and Notch signaling - regulates sex determination and dosage compensation - modulates neural functions
Gene model reviewed during 5.49
Gene model reviewed during 5.43
There is only one protein coding transcript and one polypeptide associated with this gene
Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:29535189, PubMed:28675155, PubMed:29555755). The MAC subcomplex is composed of Ime4/Mettl3 and Mettl14 (PubMed:29535189, PubMed:28675155, PubMed:29555755). The MACOM subcomplex is composed of fl(2)d, Flacc/Xio, Hakai, vir, and, in some cases of nito (PubMed:29535189, PubMed:29555755).
Gate loop 1 and gate loop 2 regions are adjacent to the S-adenosyl-L-homocysteine-binding site and display large conformational changes upon ligand-binding. They may play an important role in adenosine recognition. The interface loop contributes to the heterodimer interaction.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mettl3 using the Feature Mapper tool.
Mettl3 transcripts are detected in ovaries and testes but not in the adult male and female carcasses from which they were removed. They are detected in ovaries by in situ hybridization. Transcripts are present in the germaria and persist in later stages, where they are most prominent in follicle cells.
Mettl3 protein is detected in ovaries but not in the adult female carcasses from which they were removed. It is detected by immunolocalization in somatic (prefollicle, follicle, and polar) cells and in germline-derived cells. Protein levels are strong in the middle region of the germarium, the region where 16-cell cysts of sister germ cells begin to be surrounded by a single layer of somatic follicle cells to form the emerging egg chamber. In later egg chambers throughout oogenesis, Mettl3 protein is observed in all follicle cells, but particularly strongly in polar follicle cells. It is also detected in the ooplasm and in cells of the 16-cell cyst of early stages.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Mettl3 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Renamed from 'Ime4' to 'Mettl3' to be: (i) more informative and not wrongly imply a role in inducing meiosis, (ii) consistent with the nomenclature of the human/vertebrate ortholog, and (iii) consistent with the nomenclature of the other D. melanogaster gene encoding an 'mRNA (2-O-methyladenosine-N(6)-)-methyltransferase' (CG7818/Mettl14).
CG5933 was originally named 'Mta70' based on FBrf0211786. However, that paper did not explicitly name or focus on this gene. FBrf0215277 is the first paper to specifically characterize the gene, and so the gene nomenclature used therein has been used as the FlyBase gene symbol/name.
FlyBase curator comment: Correspondence with author Hongay revealed that 'IME4' = 'CG5933'.