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FB2025_05
,
released December 11, 2025
Ime4, Inducer of meiosis 4
methyltransferase- internal modification of mRNA - regulates alternative splicing and Notch signaling - regulates sex determination and dosage compensation - modulates neural functions
Please see the JBrowse view of Dmel\Mettl3 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.49
Gene model reviewed during 5.43
There is only one protein coding transcript and one polypeptide associated with this gene
Component of the WMM complex, a N6-methyltransferase complex composed of a catalytic subcomplex, named MAC, and of an associated subcomplex, named MACOM (PubMed:28675155, PubMed:29535189, PubMed:29555755). The MAC subcomplex is composed of Ime4/Mettl3 and Mettl14 (PubMed:28675155, PubMed:29535189, PubMed:29555755). The MACOM subcomplex is composed of fl(2)d, Flacc/Xio, Hakai, vir, and, in some cases of nito (PubMed:29535189, PubMed:29555755).
Gate loop 1 and gate loop 2 regions are adjacent to the S-adenosyl-L-homocysteine-binding site and display large conformational changes upon ligand-binding. They may play an important role in adenosine recognition. The interface loop contributes to the heterodimer interaction.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mettl3 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Mettl3 transcripts are detected in ovaries and testes but not in the adult male and female carcasses from which they were removed. They are detected in ovaries by in situ hybridization. Transcripts are present in the germaria and persist in later stages, where they are most prominent in follicle cells.
Mettl3 protein is detected in ovaries but not in the adult female carcasses from which they were removed. It is detected by immunolocalization in somatic (prefollicle, follicle, and polar) cells and in germline-derived cells. Protein levels are strong in the middle region of the germarium, the region where 16-cell cysts of sister germ cells begin to be surrounded by a single layer of somatic follicle cells to form the emerging egg chamber. In later egg chambers throughout oogenesis, Mettl3 protein is observed in all follicle cells, but particularly strongly in polar follicle cells. It is also detected in the ooplasm and in cells of the 16-cell cyst of early stages.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Mettl3 in JBrowse3-82
3-82.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
New stable cell line derived from S2-unspecified : Stable S2 Mettl3-KO cell lines were generated by CRISPR/Cas9-mediated mutatgenesis.
Deletions removing the Ime4 gene are homozygous lethal.
FlyBase curator comment: Correspondence with author Hongay revealed that 'IME4' = 'CG5933'.
Source for identity of: Mta70 CG5933
Source for identity of: Ime4 Mta70
Source for identity of: Mettl3 Ime4
Renamed from 'Ime4' to 'Mettl3' to be: (i) more informative and not wrongly imply a role in inducing meiosis, (ii) consistent with the nomenclature of the human/vertebrate ortholog, and (iii) consistent with the nomenclature of the other D. melanogaster gene encoding an 'mRNA (2-O-methyladenosine-N(6)-)-methyltransferase' (CG7818/Mettl14).
CG5933 was originally named 'Mta70' based on FBrf0211786. However, that paper did not explicitly name or focus on this gene. FBrf0215277 is the first paper to specifically characterize the gene, and so the gene nomenclature used therein has been used as the FlyBase gene symbol/name.
