Gene model reviewed during 5.47
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dis3 using the Feature Mapper tool.
Dis3 transcript can be detected by Northern analysis from 0-12 hrs AEL, but not from 12-20 hrs AEL; expression peaks at 6-8 hrs AEL. Dis3 transcript is also detected during larval, pupal, and adult stages, with a second peak during the first larval instar. Dis3 transcript is detected by in situ hybridization starting at oogenesis stage 3, and is abundant in nurse cells. Maternally deposited transcript is abundant in embryos through cellularization. Dis3 transcript is ubiquituously expressed during gastrulation, and particularly abundant in mesoderm. Expression is less instance by germ band retraction.
Dis3 protein can be detected by Western analysis from 0 hrs AEL through the third larval instar; expression peaks at 6-8 hr AEL, drops sharply at 12-24 hr AEL, and is barely detectable at larval stages. Expression is also detected in female but not male adults. Dis3 protein distribution in embyros is similar to the distribution of Dis3 transcipt; ubiquitous immunoreactivity is observed through germ band retraction, and is more pronounced in mesoderm.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Dis3 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.