FlyBase Gene Report: Dmel\REPTOR

General Information

Symbol

Dmel\REPTOR

Species

D. melanogaster

Name

Repressed by TOR

Annotation Symbol

CG13624

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039209

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Snapshot

Repressed by TOR (REPTOR) encodes a transcription factor that shuttles between cytoplasm and nucleus depending on the state of the activity of the product of Tor. Together with its binding partner encoded by REPTOR-BP, it mediates much of the transcriptional response observed upon Tor complex 1 inhibition. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

96A7-96A9

Sequence location

3R:24,557,368..24,582,864 [-]

Recombination map

3-85

RefSeq locus

NT_033777 REGION:24557368..24582864

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the bZIP family. CREBRF subfamily. (Q9VC61)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

protein heterodimerization activity

Predictions / Assertions

DNA-binding transcription factor activity

Summaries

Gene Group (FlyBase)

BASIC LEUCINE ZIPPER TRANSCRIPTION FACTORS -: The basic leucine zipper (bZIP) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60-80 amino acid bZIP domain: a basic DNA binding domain followed by a leucine zipper dimerization domain. (Adapted from FBrf0152056).

Protein Function (UniProtKB)

Transcriptional regulator that acts in the TORC1 signaling pathway to regulate energy homeostasis and promote survival during nutrient deprivation. Interacts with REPTOR to form a transcriptional activator complex that functions downstream of TORC1 to up-regulate the expression of most target genes induced by TORC1 inhibition. In the complex, acts as the transcriptional activator. Under normal conditions TORC1 is active, inhibiting the formation of the REPTOR/REPTOR-BP complex by phosphorylating REPTOR and mediates its cytoplasmic retention by forming a docking site for 14-3-3 proteins. Upon TORC1 inhibition resulting from nutrient stress, REPTOR is recruited into the nucleus where it interacts with REPTOR-BP and together they maintain organismal metabolism by activating the expression of target stress response genes including those involved in glycogenesis and triglyceride biosynthesis. The complex also appears to negatively regulate some aspects of TORC1-dependent larval growth.

(UniProt, Q9VC61)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\REPTOR or the JBrowse view of Dmel\REPTOR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.46

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

REPTOR-RA

FBtr0084674

NM_170150

3864

755

REPTOR-RB

FBtr0084672

NM_170151

5651

755

REPTOR-RC

FBtr0084675

NM_143014

4228

755

REPTOR-RD

FBtr0084673

NM_170152

3684

755

REPTOR-RE

FBtr0110795

NM_001043287

3680

814

REPTOR-RF

FBtr0110796

NM_001043288

3805

814

REPTOR-RG

FBtr0345204

NM_001300576

3698

755

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

REPTOR-PA

FBpp0084054

82.3

755

6.09

NP_733012

AAF56314

REPTOR-PB

FBpp0084052

82.3

755

6.09

NP_733013

AAN14001

REPTOR-PC

FBpp0084055

82.3

755

6.09

NP_651271

AAN14002

REPTOR-PD

FBpp0084053

82.3

755

6.09

NP_733014

AAN14003

REPTOR-PE

FBpp0110092

88.6

814

6.39

NP_001036752

ABI31203

REPTOR-PF

FBpp0110093

88.6

814

6.39

NP_001036753

ABI31204

REPTOR-PG

FBpp0311399

82.3

755

6.09

NP_001287505

AHN57504

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

755 aa isoforms: REPTOR-PA, REPTOR-PB, REPTOR-PC, REPTOR-PD, REPTOR-PG

814 aa isoforms: REPTOR-PE, REPTOR-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts (via C-terminus) with REPTOR-BP (via C-terminus). Interacts with the TORC1 complex component raptor. Interacts (when phosphorylated) with 14-3-3zeta, this interaction may assist its cytoplasmic retention.

(UniProt, Q9VC61)

Post Translational Modification

Phosphorylation by the TORC1 kinase complex at Ser-527 and Ser-530 abolishes nuclear localization. Upon TORC1 inhibition, dephosphorylated in a mts/PP2a-dependent manner leading to nuclear localization.

(UniProt, Q9VC61)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\REPTOR using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein heterodimerization activity

inferred from physical interaction with FLYBASE:REPTOR-BP; FB:FBgn0032202

(Tiebe et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR004827

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

positive regulation of transcription, DNA-templated

inferred from mutant phenotype

(Tiebe et al., 2015)

response to starvation

inferred from mutant phenotype

(Tiebe et al., 2015)

TORC1 signaling

inferred from mutant phenotype

(Tiebe et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

response to unfolded protein

inferred from electronic annotation with InterPro:IPR039165

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

cytosol

inferred from direct assay

(Tiebe et al., 2015)

nucleus

inferred from direct assay

(Tiebe et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

organism | pair rule expression pattern

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium