Gene Dmel\atl

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Guanylate-binding protein, N-terminal (IPR015894)

P-loop containing nucleoside triphosphate hydrolase (IPR027417)

Guanylate-binding protein, C-terminal (IPR003191)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VC57

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism

• cytoplasm | basal

Comment:apically cleared

embryonic stage 9 -- 10

anterior endoderm

inclusive hindgut primordium

organism | faint & ubiquitous

posterior endoderm

trunk mesoderm

embryonic stage 11 -- 12

anterior midgut primordium

foregut primordium

posterior midgut primordium

salivary gland body primordium

somatic muscle primordium

trunk mesoderm

visceral muscle primordium

embryonic stage 13 -- 16

embryonic dorsal epidermis

embryonic foregut

embryonic head epidermis

embryonic hindgut

embryonic ventral epidermis

embryonic/larval midgut

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval brain

ventral nerve cord

embryonic/larval somatic muscle

adult stage

adult brain | ubiquitous

dopaminergic neuron | subset of adult brain

oogenesis stage S5 -- S6

egg chamber | posterior compartment

nurse cell

• membrane

nurse cell

• cytoplasm

oogenesis stage S9

oocyte

• cytoplasm | anterior

oogenesis stage S10

follicle cell

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

In early oogenesis, atl protein is expressed in the posterior compartment of the egg chamber and in the membrane and cytoplasm of nurse cells. At oogenesis stage 9 it is found in the anterior cytoplasm of the oocyte. It then disapeears from this domain and at stage 10 atl protein is strongly expressed in follicle cells. In the adult brain it is expressed ubiquitously, with some cellls, including a subset of dopaminergic neurons expressing atl strongly.

atl is detected in neuropil regions of the brain and ventral nerve cord of third instar larvae. It is also detected in motor axon bundles emanating from the ventral ganglia. It is highly expressed throughout the cytolasm of body wall muscles and present at lower levels in the proximal parts of presynaptic arbors. It is not present in synaptic boutons.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

References

cytoplasm

inferred from direct assay

endoplasmic reticulum

inferred from direct assay

Golgi apparatus

inferred from direct assay

intrinsic to endoplasmic reticulum membrane

inferred from direct assay

(The modENCODE Consortium, 2010, Graveley et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult brain, adult ovary, adult testis.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		1799.3
Larval Trachea		1491.925
Larval Carcass		1331.125
Adult Head		851.2
Adult Eye		1127.15
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		1867.6
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		2544.3
Adult Salivary Gland		1291.7
Adult Heart		3277.025
Adult VirginFemale Spermatheca		1422.5
Adult InseminatedFemale Spermatheca		1788.8
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		1167.1
Adult Carcass		1905.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(659.4)
Larval Midgut		(963)
Larval Hindgut		(686.7)
Larval Malpighian Tubules		(938.3)
Larval Fat Body		(950.4)
Larval Salivary Gland		(1799.3)
Larval Trachea		(1491.925)
Larval Carcass		(1331.125)
Adult Head		(851.2)
Adult Eye		(1127.15)
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		(584.4)
Adult Crop		(1867.6)
Adult Midgut		(1000)
Adult Hindgut		(905.4)
Adult Malpighian Tubules		(645.7)
Adult Fat Body		(2544.3)
Adult Salivary Gland		(1291.7)
Adult Heart		(3277.025)
Adult VirginFemale Spermatheca		(1422.5)
Adult InseminatedFemale Spermatheca		(1788.8)
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		(1167.1)
Adult Carcass		(1905.5)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		(1799.3)
Larval Trachea		(1491.925)
Larval Carcass		(1331.125)
Adult Head		851.2
Adult Eye		(1127.15)
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		(1867.6)
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		(2544.3)
Adult Salivary Gland		(1291.7)
Adult Heart		(3277.025)
Adult VirginFemale Spermatheca		(1422.5)
Adult InseminatedFemale Spermatheca		(1788.8)
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		(1167.1)
Adult Carcass		(1905.5)
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		1799.3
Larval Trachea		1491.925
Larval Carcass		1331.125
Adult Head		851.2
Adult Eye		1127.15
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		1867.6
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		2544.3
Adult Salivary Gland		1291.7
Adult Heart		3277.025
Adult VirginFemale Spermatheca		1422.5
Adult InseminatedFemale Spermatheca		1788.8
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		1167.1
Adult Carcass		1905.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		1799.3
Larval Trachea		1491.925
Larval Carcass		1331.125
Adult Head		851.2
Adult Eye		1127.15
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		1867.6
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		2544.3
Adult Salivary Gland		1291.7
Adult Heart		3277.025
Adult VirginFemale Spermatheca		1422.5
Adult InseminatedFemale Spermatheca		1788.8
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		1167.1
Adult Carcass		1905.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		(963)
Larval Hindgut		686.7
Larval Malpighian Tubules		(938.3)
Larval Fat Body		(950.4)
Larval Salivary Gland		(1799.3)
Larval Trachea		(1491.925)
Larval Carcass		(1331.125)
Adult Head		(851.2)
Adult Eye		(1127.15)
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		(1867.6)
Adult Midgut		(1000)
Adult Hindgut		(905.4)
Adult Malpighian Tubules		645.7
Adult Fat Body		(2544.3)
Adult Salivary Gland		(1291.7)
Adult Heart		(3277.025)
Adult VirginFemale Spermatheca		(1422.5)
Adult InseminatedFemale Spermatheca		(1788.8)
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		(1167.1)
Adult Carcass		(1905.5)
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		(1799.3)
Larval Trachea		1491.925
Larval Carcass		1331.125
Adult Head		851.2
Adult Eye		1127.15
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		(1867.6)
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		(2544.3)
Adult Salivary Gland		1291.7
Adult Heart		(3277.025)
Adult VirginFemale Spermatheca		1422.5
Adult InseminatedFemale Spermatheca		(1788.8)
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		1167.1
Adult Carcass		(1905.5)
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		659.4
Larval Midgut		963
Larval Hindgut		686.7
Larval Malpighian Tubules		938.3
Larval Fat Body		950.4
Larval Salivary Gland		1799.3
Larval Trachea		1491.925
Larval Carcass		1331.125
Adult Head		851.2
Adult Eye		1127.15
Adult Brain		463.9
Adult Thoracic-Abdominal Ganglion		584.4
Adult Crop		1867.6
Adult Midgut		1000
Adult Hindgut		905.4
Adult Malpighian Tubules		645.7
Adult Fat Body		2544.3
Adult Salivary Gland		1291.7
Adult Heart		3277.025
Adult VirginFemale Spermatheca		1422.5
Adult InseminatedFemale Spermatheca		1788.8
Adult Ovary		431
Adult Testis		211.3
Adult Male Accessory Gland		1167.1
Adult Carcass		1905.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	851.2
NA	Eye	1127.15
NA	Brain	463.9
659.4	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	584.4
NA	Crop	1867.6
963	Midgut	1000
686.7	Hindgut	905.4
938.3	Malpighian Tubules	645.7
950.4	Fat Body	2544.3
1799.3	Salivary Gland	1291.7
NA	Heart	3277.025
1491.925	Trachea	NA
NA	VirginFemale Spermatheca	1422.5
NA	InseminatedFemale Spermatheca	1788.8
NA	Ovary	431
NA	Testis	211.3
NA	Male Accessory Gland	1167.1
1331.125	Carcass	1905.5

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(60)
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		(30)
head, virgin 4-day female		26
head, virgin 20-day female		(31)
head, mated 1-day female		(30)
head, mated 4-day female		25
head, mated 20-day female		(28)
head, mated 1-day male		(34)
head, mated 4-day male		(34)
head, mated 20-day male		(41)
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		(62)
digestive system, 1-day adult		(32)
digestive system, 4-day adult		(27)
digestive system, 20-day adult		(36)
fat body, larvae L3 wandering		(56)
fat body, white prepupae		(28)
fat body, pupae P8		24
carcass, larvae L3 wandering		(62)
carcass, 1-day adult		(56)
carcass, 4-day adult		(57)
carcass, 20-day adult		(68)
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(35)
testis, mated 4-day male		(76)
accessory gland, mated 4-day male		(31)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(60)
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		(34)
head, mated 4-day male		(34)
head, mated 20-day male		(41)
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		(62)
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		(36)
fat body, larvae L3 wandering		(56)
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		(62)
carcass, 1-day adult		(56)
carcass, 4-day adult		(57)
carcass, 20-day adult		(68)
ovary, virgin 4-day female		(42)
ovary, mated 4-day female		(35)
testis, mated 4-day male		(76)
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		60
central nervous system, larvae L3		26
central nervous system, pupae P8		21
head, virgin 1-day female		30
head, virgin 4-day female		26
head, virgin 20-day female		31
head, mated 1-day female		30
head, mated 4-day female		25
head, mated 20-day female		28
head, mated 1-day male		34
head, mated 4-day male		34
head, mated 20-day male		41
salivary gland, larvae L3 wandering		14
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		62
digestive system, 1-day adult		32
digestive system, 4-day adult		27
digestive system, 20-day adult		36
fat body, larvae L3 wandering		56
fat body, white prepupae		28
fat body, pupae P8		24
carcass, larvae L3 wandering		62
carcass, 1-day adult		56
carcass, 4-day adult		57
carcass, 20-day adult		68
ovary, virgin 4-day female		42
ovary, mated 4-day female		35
testis, mated 4-day male		76
accessory gland, mated 4-day male		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-18 hour embryonic stages, during late larval stages, during early pupal stages, in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(51)
embryo 02-04hr		(54)
embryo 04-06hr		(28)
embryo 06-08hr		(44)
embryo 08-10hr		(53)
embryo 10-12hr		(81)
embryo 12-14hr		(62)
embryo 14-16hr		(58)
embryo 16-18hr		(56)
embryo 18-20hr		(52)
embryo 20-22hr		(44)
embryo 22-24hr		(44)
larva L1		(28)
larva L2		(45)
larva L3 12hr old		(35)
larva L3 puffstage 1-2		(42)
larva L3 puffstage 3-6		(66)
larva L3 puffstage 7-9		(68)
white prepupae new		(71)
white prepupae 12hr		(45)
white prepupae 24hr		(39)
pupae 2d postWPP		(48)
pupae 3d postWPP		(48)
pupae 4d postWPP		(48)
adult male 01day		(60)
adult male 05day		(72)
adult male 30day		(60)
adult female 01day		(48)
adult female 05day		(47)
adult female 30day		(47)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(51)
embryo 02-04hr		(54)
embryo 04-06hr		28
embryo 06-08hr		(44)
embryo 08-10hr		(53)
embryo 10-12hr		(81)
embryo 12-14hr		(62)
embryo 14-16hr		(58)
embryo 16-18hr		(56)
embryo 18-20hr		(52)
embryo 20-22hr		(44)
embryo 22-24hr		(44)
larva L1		28
larva L2		(45)
larva L3 12hr old		(35)
larva L3 puffstage 1-2		(42)
larva L3 puffstage 3-6		(66)
larva L3 puffstage 7-9		(68)
white prepupae new		(71)
white prepupae 12hr		(45)
white prepupae 24hr		(39)
pupae 2d postWPP		(48)
pupae 3d postWPP		(48)
pupae 4d postWPP		(48)
adult male 01day		(60)
adult male 05day		(72)
adult male 30day		(60)
adult female 01day		(48)
adult female 05day		(47)
adult female 30day		(47)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		51
embryo 02-04hr		54
embryo 04-06hr		28
embryo 06-08hr		44
embryo 08-10hr		53
embryo 10-12hr		81
embryo 12-14hr		62
embryo 14-16hr		58
embryo 16-18hr		56
embryo 18-20hr		52
embryo 20-22hr		44
embryo 22-24hr		44
larva L1		28
larva L2		45
larva L3 12hr old		35
larva L3 puffstage 1-2		42
larva L3 puffstage 3-6		66
larva L3 puffstage 7-9		68
white prepupae new		71
white prepupae 12hr		45
white prepupae 24hr		39
pupae 2d postWPP		48
pupae 3d postWPP		48
pupae 4d postWPP		48
adult male 01day		60
adult male 05day		72
adult male 30day		60
adult female 01day		48
adult female 05day		47
adult female 30day		47
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		166
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(56)
Schneider line 2 Sg4		(47)
embryonic 1182-4H		(41)
embryonic GM2		(58)
embryonic Kc167		(45)
embryonic S1		(166)
embryonic S3		(41)
leg disc CME L1		(41)
wing disc CME-W2		(36)
wing disc ML-DmD8		25
wing disc ML-DmD9		(38)
wing disc ML-DmD16-c3		(29)
wing disc ML-DmD21		(42)
wing disc ML-DmD32		(47)
haltere disc ML-DmD17-c3		(29)
eye-antennal disc ML-DmD11		(38)
antennal disc ML-DmD20-c5		(35)
mixed discs ML-DmD4-c1		(29)
CNS ML-DmBG1-c1		(31)
CNS ML-DmBG2-c2		(42)
tumorous blood cells mbn2		(70)
ovary fGS/OSS		(52)
ovary OSC		(35)
ovary OSS		(37)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		(166)
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		166
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		166
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(56)
Schneider line 2 Sg4		(47)
embryonic 1182-4H		(41)
embryonic GM2		(58)
embryonic Kc167		(45)
embryonic S1		(166)
embryonic S3		(41)
leg disc CME L1		(41)
wing disc CME-W2		(36)
wing disc ML-DmD8		25
wing disc ML-DmD9		(38)
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		(42)
wing disc ML-DmD32		(47)
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		(38)
antennal disc ML-DmD20-c5		(35)
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		(42)
tumorous blood cells mbn2		(70)
ovary fGS/OSS		(52)
ovary OSC		(35)
ovary OSS		(37)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		(166)
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		56
Schneider line 2 Sg4		47
embryonic 1182-4H		41
embryonic GM2		58
embryonic Kc167		45
embryonic S1		166
embryonic S3		41
leg disc CME L1		41
wing disc CME-W2		36
wing disc ML-DmD8		25
wing disc ML-DmD9		38
wing disc ML-DmD16-c3		29
wing disc ML-DmD21		42
wing disc ML-DmD32		47
haltere disc ML-DmD17-c3		29
eye-antennal disc ML-DmD11		38
antennal disc ML-DmD20-c5		35
mixed discs ML-DmD4-c1		29
CNS ML-DmBG1-c1		31
CNS ML-DmBG2-c2		42
tumorous blood cells mbn2		70
ovary fGS/OSS		52
ovary OSC		35
ovary OSS		37
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(32)
cold shock, 4-day adult		26
heat shock, 4-day adult		(36)
Cadmium 50 mM 6 hrs, larvae L3		(35)
Cadmium 50 mM 12 hrs, larvae L3		(41)
Cadmium 50 mM 48 hrs, 4-day adult		(29)
Cadmium 100 mM 48 hrs, 4-day adult		(42)
Copper 0.5 mM 12 hrs, larvae L3		(32)
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		(33)
Ethanol 2.5% 3 hrs, larvae L3		(48)
Ethanol 5% 3 hrs, larvae L3		(33)
Ethanol 10% 3 hrs, larvae L3		(31)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(40)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(27)
Paraquat 5 mM 48 hrs, 4-day adult		(42)
Paraquat 10 mM 48 hrs, 4-day adult		(60)
Rotenone 2 μg 12 hrs, larvae L3		(33)
Rotenone 8 μg 12 hrs, larvae L3		(38)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		(36)
Cadmium 50 mM 6 hrs, larvae L3		(35)
Cadmium 50 mM 12 hrs, larvae L3		(41)
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		(42)
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		(33)
Ethanol 2.5% 3 hrs, larvae L3		(48)
Ethanol 5% 3 hrs, larvae L3		(33)
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(40)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(39)
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		(42)
Paraquat 10 mM 48 hrs, 4-day adult		(60)
Rotenone 2 μg 12 hrs, larvae L3		(33)
Rotenone 8 μg 12 hrs, larvae L3		(38)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		32
cold shock, 4-day adult		26
heat shock, 4-day adult		36
Cadmium 50 mM 6 hrs, larvae L3		35
Cadmium 50 mM 12 hrs, larvae L3		41
Cadmium 50 mM 48 hrs, 4-day adult		29
Cadmium 100 mM 48 hrs, 4-day adult		42
Copper 0.5 mM 12 hrs, larvae L3		32
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		26
Zinc 4.5 mM 48 hrs, 4-day adult		33
Ethanol 2.5% 3 hrs, larvae L3		48
Ethanol 5% 3 hrs, larvae L3		33
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		40
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		39
Caffeine 25 mg/ml 48 hrs, 4-day adult		27
Paraquat 5 mM 48 hrs, 4-day adult		42
Paraquat 10 mM 48 hrs, 4-day adult		60
Rotenone 2 μg 12 hrs, larvae L3		33
Rotenone 8 μg 12 hrs, larvae L3		38
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for atl

BDGP expression data

•

GH09383

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0039213

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG6668-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

atl^GD242

lethal, with Scer\GAL4^unspecified

atl^{Scer\UAS.T:Hsap\MYC}

lethal | pupal stage, with Scer\GAL4^pnr-MD237

atl^GD242

lethal | pupal stage (with Df(3R)Exel7357)

atl²

lethal | pupal stage | recessive

atl²

viable

Sterility

Allele

fertile

sterile | recessive

Other Phenotypes

Allele

bang sensitive

atl¹

bang sensitive, with Scer\GAL4^ple.PF

locomotor behavior defective

atl¹

neuroanatomy defective | larval stage

atl²

neuroanatomy defective | larval stage, with Scer\GAL4^C57

atl^{R192Q.Scer\UAS}

neuroanatomy defective | larval stage (with Df(3R)Exel7357)

atl²

short lived

atl¹

small body | adult stage

atl¹

small body | pupal stage

atl¹

small body | recessive

atl²

Phenotype manifest in

Allele

abdominal 2 ventral longitudinal muscle 3 (with Df(3R)Exel7357)

atl²

abdominal 2 ventral longitudinal muscle 4 (with Df(3R)Exel7357)

atl²

dopaminergic neuron

atl¹

dopaminergic neuron, with Scer\GAL4^ple.PF

embryonic/larval somatic muscle, with Scer\GAL4^C57

atl^Scer\UAS.cLa

endoplasmic reticulum

atl¹

endoplasmic reticulum, with Scer\GAL4^D42

atl^{Scer\UAS.T:Hsap\MYC}

endoplasmic reticulum, with Scer\GAL4^αTub84B.PL

atl^{dsRNA.Scer\UAS}

imaginal disc | somatic clone

atl¹

microtubule & embryonic/larval somatic muscle, with Scer\GAL4^C57

microtubule & embryonic/larval somatic muscle (with Df(3R)Exel7357)

atl²

NMJ bouton

atl²

NMJ bouton, with Scer\GAL4^C57

atl^{R192Q.Scer\UAS}

NMJ bouton (with Df(3R)Exel7357)

atl²

oocyte

atl¹

postsynaptic membrane (with Df(3R)Exel7357)

atl²

Classical Alleles ( 13 )

For All Classical Alleles Show

Allele of atl	Class	Mutagen	Stocks	Known lesion
atl^5-SZ-3256			1	--
atl^EY07916		P-element activity	1	--
atl^EY16768		P-element activity	1	--
atl^MB07599			1	--
atl^MI00863			1	--
atl^NP2088			1	--
atl^NP2644			1	--
atl^NP5390			1	--
atl^NP5434			1	--
atl¹			0	Yes
atl²	loss of function allele	P-element activity	0	Yes
atl^B101			0	--
atl^d02871		P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of atl	Mutagen	Stocks	Known lesion
atl^GD242	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
atl^HMS01627	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
atl^+t13	in vitro construct - genomic fragment	0	Yes
atl^{dsRNA.Scer\UAS}	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
atl^{K51A.Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
atl^{R192Q.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
atl^{R214C.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - amino acid replacement	0	Yes
atl^Scer\UAS.cLa	in vitro construct - regulatory fusion	0	Yes
atl^{Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel7357

(Ryder, 2004.4.26, Lee et al., 2009)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{atl^+t13}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

miscellaneous insertions

Mi{MIC}atl^MI00863

P{RS5}atl^5-SZ-3256

PBac{5HPw⁺}atl^B101

insertion of enhancer trap binary system

Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )

Terms Based on Experimental Evidence ( 12 terms )

Molecular Function

CV term

References

GTPase activity

inferred from direct assay

inferred from mutant phenotype

guanyl ribonucleotide binding

inferred from mutant phenotype

protein homodimerization activity

inferred from mutant phenotype

Biological Process

CV term

endoplasmic reticulum membrane fusion

References

inferred from direct assay

inferred from mutant phenotype

membrane fusion

inferred from direct assay

neuromuscular junction development

inferred from mutant phenotype

regulation of microtubule depolymerization

inferred from mutant phenotype

regulation of synaptic growth at neuromuscular junction

inferred from mutant phenotype

Cellular Component

CV term

References

cytoplasm

inferred from direct assay

endoplasmic reticulum

inferred from direct assay

Golgi apparatus

inferred from direct assay

intrinsic to endoplasmic reticulum membrane

inferred from direct assay

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function

CV term

(FlyBase Curators et al., 2004-)

References

GTP binding

inferred from electronic annotation with InterPro:IPR003191, InterPro:IPR015894

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

atl - CG1105

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

atl - Mul1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

atl - spas

pull down, anti tag western blot

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

atl allele

Gene

References

External Data

y¹ w^67c23; P{EPgy2}atl^EY07916

BioGRID - A database of protein and genetic interactions

•

67860

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0039213

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42934

Orthologs

OrthoDB Orthologs (53) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG61RQDN

Dipteran inclusive ortholog search

EOG6VHKFS

Insect inclusive ortholog search

EOG6Q57JR

Arthropod inclusive ortholog search

EOG6H70SW

Metazoa inclusive ortholog search

EOG6TJ7M6

Orthologs in Drosophila Species (EOG61RQDN)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19766

Drosophila persimilis

Dper\GL13613

Drosophila willistoni

Dwil\GK10964

Drosophila virilis

Dvir\GJ23644

Drosophila mojavensis

Dmoj\GI24013

Drosophila grimshawi

Dgri\GH18437

Orthologs in non-Drosophila Dipterans (EOG6VHKFS)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003109

Anopheles gambiae

Malaria mosquito

Agam\AGAP002047

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008647

Orthologs in non-Dipteran Insects (EOG6Q57JR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB44396

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006190

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH10922

Atta cephalotes

Leafcutter ant

Acep\ACEP20126

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO13971

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL16420

Linepithema humile

Argentine ant

Lhum\LH18317

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB26056

Solenopsis invicta

Red fire ant

Sinv\SINV11291

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI068591

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002537

Bombyx mori

Silkmoth

Bmor\BGIBMGA000802

Bombyx mori

Silkmoth

Bmor\BGIBMGA000767

Pediculus humanus

Human body louse

Phum\PHUM452510

Tribolium castaneum

Red flour beetle

Tcas\TC009975

Orthologs in non-Insect Arthropods (EOG6H70SW)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\Odf4

Ixodes scapularis

Deer tick

Isca\ISCW003306

Orthologs in non-Arthropod Metazoa (EOG6TJ7M6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\4D561

Caenorhabditis elegans

Nematode

Cele\Y41E3.3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_011773gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016744gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016743gn

Danio rerio

Zebrafish

Drer\atl1

Danio rerio

Zebrafish

Drer\arl6ip2

Xenopus tropicalis

Western clawed frog

Xtro\atl2

Xenopus tropicalis

Western clawed frog

Xtro\atl1

Xenopus tropicalis

Western clawed frog

Xtro\atl3

Gallus gallus

Domestic chicken

Ggal\ATL2

Gallus gallus

Domestic chicken

Ggal\ATL1

Mus musculus

House mouse

Mmus\Atl2

Mus musculus

House mouse

Mmus\Atl1

Mus musculus

House mouse

Mmus\Atl3

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000021203

Rattus norvegicus

Norway rat

Rnor\Atl1

Rattus norvegicus

Norway rat

Rnor\Atl2

Homo sapiens

Human

Hsap\ATL1

Homo sapiens

Human

Hsap\ATL2

Homo sapiens

Human

Hsap\ATL3

Human Orthologs (3)

Gene

OMIM

HGNC

Hsap\ATL1

Hsap\ATL2

Hsap\ATL3

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19766

Drosophila persimilis

Dper\GL13613

Drosophila willistoni

Dwil\GK10964

Drosophila virilis

Dvir\GJ23644

Drosophila mojavensis

Dmoj\GI24013

Drosophila grimshawi

Dgri\GH18437

Stocks & Reagents

Stocks Listed in FlyBase ( 11 )

Bloomington

37303

y¹ w^*; Mi{MIC}atl^MI00863

17414

21236

y¹ w^67c23; P{EPgy2}atl^EY16768

36736

y¹ sc^* v¹; P{TRiP.HMS01627}attP40

25560

w¹¹¹⁸; Mi{ET1}atl^MB07599/TM6C, Sb¹

Kyoto

125963

w¹¹¹⁸; P{RS5}atl^5-SZ-3256

113713

w^*; P{GawB}atl^NP5390 / TM6, P{UAS-lacZ.UW23-1}UW23-1

104083

w^*; P{GawB}atl^NP2088 / TM3, Sb¹ Ser¹

112990

w^*; P{GawB}atl^NP2644 / TM3, Sb¹ Ser¹

105010

y^* w^*; P{GawB}atl^NP5434 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v6719

w¹¹¹⁸; P{GD242}v6719

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 272 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information