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Gene Dmel\nct

General Information
Symbol	Dmel\nct	Species	D. melanogaster
Name	nicastrin	Annotation symbol	CG7012
Feature type	protein_coding_gene	FlyBase ID	FBgn0039234
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	Nic, agro
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96B1-96B1	Sequence location	3R:20,677,294..20,680,730 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nicastrin is referred to in FlyBase by the symbol Dmel\nct (CG7012, FBgn0039234). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: Notch receptor processing; regulation of compound eye photoreceptor development; lateral inhibition; membrane protein ectodomain proteolysis; Notch signaling pathway; R8 cell development; photoreceptor cell morphogenesis; cytoskeleton organization. 36 alleles are reported. The phenotypes of these alleles are annotated with: larva; eye; wing disc. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Nicastrin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval midgut, larval hindgut, larval Malpighian tubules, larval fat body, larval salivary gland, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1640412_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of nct. Gene sequence location is 3R:20677294..20680730.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN20273 HFA16172
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96B1-96B1   Limits computationally determined from genome sequence between P{PZ}crb07207&P{PZ}BRWD305842 and P{EP}CycB3EP3127
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96A13-96B15     (Yu, 2000.3.2)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nct for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nct-RA FBtr0084705  NM_143040   2438   695 nct-RC FBtr0301865  NM_001170242   2936   695 nct-RD FBtr0306087     2513   692
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nct-PA   FBpp0084083   78.0   695   5.94     NP_651297   AAF56349 nct-PC   FBpp0291079   78.0   695   5.94     NP_001163713   ACZ95007 nct-PD   FBpp0297224   77.7   692   5.94
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Nicastrin (IPR008710)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202070   AA246654   AA264879   AA391131   AA391767   AA438411   AA536335   AA536337   AA539440   AA539446   AA696320   AA820574   AA820782   AA940620   AA949165   AA949763   AA979248   AA979543   AA979703   AE014297 AAF56349   AE014297 ACZ95007   AF240470 AAG11414   AI294482   AI455052   AI456701   AI519135   AI533335   AI541950   AI542345   AI546155   AW943356   BF495671   BI163357   BI165155   BI172782   BI212257   BI228600   BI228601   BI229738   BI234193   BI238102   BI240957   BI361309   BI362183   BI362785   BI363077   BI364040   BI367307   BI369539   BI373133   BI482890   BI578317   BI579730   BI639737   BI641144   BT003245 AAO25002   CA807387   CK657044   CK657176   CO154917   CO179220   CO187041   CO192410   CO194014   CO263963   CO269874   CO276254   CO276929   CO279715   CO284664   CO289819   CO290678   CO294031   CO294331   CO299566   CO300879   CO303006   CO306747   CO308601   CO309161   CO320832   CO328865   CO335396   DN154312   EC060019   EC062719   EC062918   EC071689   EC072315   EC078659   EC082649   EC085169   EC089150   EC090125   EC212762   EC213721   EC217775   EC219237   EC221122   EC230764   EC234964   EC242550   EC247325   EC249189   EC250926   EC261034   EC264946   EV600391   GH748237   GH748511   GH751949   GH755187   GH756892   GH760492   GH761373   GH772390   GH772579   GH772955   GH779089   GH785883   GH786115   GH786556   GH787797   GH788370   GH790426   GH791547   GH794307   GH796810   GH797602   GH801870   GH803883   GH804041   GH804647   GH806745   GH809563   GH809704   GH812502   GH812937   GH814406   GH814999   GH816839   GH820949   GH820969   GH824919   GH825328   GH828393   GH832942   GH833870   GH834968   GH835837   GH841403   GH842697   GH842838   GH843196   GH847416   GH851953   GH852955   GH853350   GH853753   GH857208   GH860072   GH863621   GH865500   GH867767   GH868273   GH873366   GH874696   GH874806   GH877754   GH879248   GH880402   GH881445   GH884512   GH885126   GH886986   GH887132   GH889690   GH891158   GH891955   GH892700   GH895345   GH896309   GH898654   GH902214   GH908809   GH909058   GH909968   GH913140   GH916291   GH917545   GH919617   GH922561   GH930501   GH936976   GH937443
UniProtKB/Swiss-Prot	Q9VC27
UniProtKB/TrEMBL	Q7KS07 E1JIV8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0039234    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0039234 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0039234 expression level Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4422) embryo 02-04hr  (6296) embryo 04-06hr  (5473) embryo 06-08hr  (3663) embryo 08-10hr  (3480) embryo 10-12hr  (2070) embryo 12-14hr  (2760) embryo 14-16hr  (2080) embryo 16-18hr  (1734) embryo 18-20hr  (1002) embryo 20-22hr  (1958) embryo 22-24hr  (2090) larva L1  (668) larva L2  (1033) larva L3 12hr old  (782) larva L3 puffstage 1-2  (1281) larva L3 puffstage 3-6  (2491) larva L3 puffstage 7-9  (3970) white prepupae new  (4606) white prepupae 12hr  (3790) white prepupae 24hr  (3431) pupae 2d postWPP  (2806) pupae 3d postWPP  (1154) pupae 4d postWPP  (1067) adult male 01day  (1071) adult male 05day  (1063) adult male 30day  (1016) adult female 01day  (1542) adult female 05day  (2617) adult female 30day  (2605) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4422) embryo 02-04hr  (6296) embryo 04-06hr  (5473) embryo 06-08hr  (3663) embryo 08-10hr  (3480) embryo 10-12hr  (2070) embryo 12-14hr  (2760) embryo 14-16hr  (2080) embryo 16-18hr  (1734) embryo 18-20hr    1002 embryo 20-22hr  (1958) embryo 22-24hr  (2090) larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6  (2491) larva L3 puffstage 7-9  (3970) white prepupae new  (4606) white prepupae 12hr  (3790) white prepupae 24hr  (3431) pupae 2d postWPP  (2806) pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day  (1542) adult female 05day  (2617) adult female 30day  (2605) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0039234 expression level Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4422) embryo 02-04hr  (6296) embryo 04-06hr  (5473) embryo 06-08hr  (3663) embryo 08-10hr  (3480) embryo 10-12hr  (2070) embryo 12-14hr  (2760) embryo 14-16hr  (2080) embryo 16-18hr  (1734) embryo 18-20hr  (1002) embryo 20-22hr  (1958) embryo 22-24hr  (2090) larva L1  (668) larva L2  (1033) larva L3 12hr old  (782) larva L3 puffstage 1-2  (1281) larva L3 puffstage 3-6  (2491) larva L3 puffstage 7-9  (3970) white prepupae new  (4606) white prepupae 12hr  (3790) white prepupae 24hr  (3431) pupae 2d postWPP  (2806) pupae 3d postWPP  (1154) pupae 4d postWPP  (1067) adult male 01day  (1071) adult male 05day  (1063) adult male 30day  (1016) adult female 01day  (1542) adult female 05day  (2617) adult female 30day  (2605) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4422) embryo 02-04hr  (6296) embryo 04-06hr  (5473) embryo 06-08hr  (3663) embryo 08-10hr  (3480) embryo 10-12hr  2070 embryo 12-14hr  (2760) embryo 14-16hr  2080 embryo 16-18hr  1734 embryo 18-20hr    1002 embryo 20-22hr  1958 embryo 22-24hr  2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6  (2491) larva L3 puffstage 7-9  (3970) white prepupae new  (4606) white prepupae 12hr  (3790) white prepupae 24hr  (3431) pupae 2d postWPP  (2806) pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day  1542 adult female 05day  (2617) adult female 30day  (2605) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4422 embryo 02-04hr    6296 embryo 04-06hr    5473 embryo 06-08hr    3663 embryo 08-10hr    3480 embryo 10-12hr    2070 embryo 12-14hr    2760 embryo 14-16hr    2080 embryo 16-18hr    1734 embryo 18-20hr    1002 embryo 20-22hr    1958 embryo 22-24hr    2090 larva L1    668 larva L2    1033 larva L3 12hr old    782 larva L3 puffstage 1-2    1281 larva L3 puffstage 3-6    2491 larva L3 puffstage 7-9    3970 white prepupae new    4606 white prepupae 12hr    3790 white prepupae 24hr    3431 pupae 2d postWPP    2806 pupae 3d postWPP    1154 pupae 4d postWPP    1067 adult male 01day    1071 adult male 05day    1063 adult male 30day    1016 adult female 01day    1542 adult female 05day    2617 adult female 30day    2605 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0039234    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0039234 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult crop, larval midgut, larval hindgut, larval Malpighian tubules, larval fat body, larval salivary gland, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0039234 expression level Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0039234 expression level Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    326.625 Larval Midgut    206.2 Larval Hindgut    197.5 Larval Malpighian Tubules    354.2 Larval Fat Body    245.6 Larval Salivary Gland    134.3 Larval Trachea    72.975 Larval Carcass    118.075 Adult Head    86.4 Adult Eye    97.65 Adult Brain    103.4 Adult Thoracic-Abdominal Ganglion    129.9 Adult Crop    113.8 Adult Midgut    95.1 Adult Hindgut    76.7 Adult Malpighian Tubules    89 Adult Fat Body    76 Adult Salivary Gland    98.6 Adult Heart    57.35 Adult VirginFemale Spermatheca    98.4 Adult InseminatedFemale Spermatheca    103.5 Adult Ovary    380.2 Adult Testis    29.4 Adult Male Accessory Gland    139.7 Adult Carcass    44.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    86.4   NA  Eye    97.65   NA  Brain    103.4   326.625  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    129.9   NA  Crop    113.8   206.2  Midgut    95.1   197.5  Hindgut    76.7   354.2  Malpighian Tubules    89   245.6  Fat Body    76   134.3  Salivary Gland    98.6   NA  Heart    57.35   72.975  Trachea    NA   NA  VirginFemale Spermatheca    98.4   NA  InseminatedFemale Spermatheca    103.5   NA  Ovary    380.2   NA  Testis    29.4   NA  Male Accessory Gland    139.7   118.075  Carcass    44.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039234 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7012-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for nct Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal nct5P3 nct6F2 lethal | embryonic stage | germline clone | neurogenic | recessive nctA7 lethal | germline clone | neurogenic | recessive nctagro lethal | larval stage | recessive nctA7 nctJ1 nctJ2 lethal | pupal stage nctST1 nctST2 nctST3 nctST4 lethal | pupal stage (with nctST1) nctST2 nctST3 nctST4 lethal | pupal stage (with nctST2) nctST1 lethal | pupal stage (with nctST3) nctST1 lethal | pupal stage (with nctST4) nctST1 lethal | recessive nctagro nctE3 nctR46 viable nctPIE-6 Other Phenotypes Allele decreased cell number | somatic clone nctST1 nctST2 increased cell number (with nctST1) nctST3 increased cell number (with nctST3) nctST1 increased cell number | somatic clone nctST1 nctST2 neuroanatomy defective (with nctST1) nctST3 neuroanatomy defective (with nctST3) nctST1 neuroanatomy defective | somatic clone nctST1 nctST2 visible nctPIE-6 visible | recessive | somatic clone nct5P3 wild-type nct+t7 Phenotype manifest in Allele adult head | somatic clone nct5P3 antennal disc | wandering third instar larval stage (with nctST1) nctST3 antennal disc | wandering third instar larval stage (with nctST3) nctST1 cytoskeleton & follicle cell | somatic clone nctagro embryonic epidermis | germline clone nctagro external sensory organ precursor cell | supernumerary | wandering third instar larval stage (with nctST1) nctST3 external sensory organ precursor cell | supernumerary | wandering third instar larval stage (with nctST3) nctST1 eye nctPIE-6 eye disc | somatic clone | wandering third instar larval stage nctST1 nctST3 eye disc | wandering third instar larval stage nctST1 nctST2 eye disc | wandering third instar larval stage (with Df(3R)96ST) nctST1 eye disc | wandering third instar larval stage (with nctST1) nctST3 eye disc | wandering third instar larval stage (with nctST3) nctST1 eye photoreceptor cell | somatic clone | supernumerary | wandering third instar larval stage nctST1 nctST2 nctST3 eye photoreceptor cell | somatic clone | wandering third instar larval stage nctST1 nctST2 nctST3 eye photoreceptor cell | wandering third instar larval stage (with nctST1) nctST3 eye photoreceptor cell | wandering third instar larval stage (with nctST3) nctST1 follicle cell | somatic clone nctagro germinal vesicle | cell non-autonomous nctagro larva nctA7 nctJ1 nctJ2 microchaeta | somatic clone nctA7 neuroblast | embryonic stage | germline clone nctR46 nucleus | somatic clone | wandering third instar larval stage nctST1 nctST2 oocyte | cell non-autonomous nctagro photoreceptor cell R8 | somatic clone | supernumerary | wandering third instar larval stage nctST1 nctST2 nctST3 presumptive embryonic/larval central nervous system | germline clone nctA7 presumptive embryonic/larval nervous system | germline clone nctagro sensory mother cell & dorsal mesothoracic disc nctA7 nctJ1 nctJ2 ventral thoracic disc | wandering third instar larval stage (with nctST1) nctST3 ventral thoracic disc | wandering third instar larval stage (with nctST3) nctST1 wing | somatic clone nctA7 nctagro wing disc nctA7 wing disc | somatic clone nctagro wing margin | somatic clone nctA7 nctR46 nctagro wing margin bristle | somatic clone nctagro wing vein | somatic clone nctA7 nctR46 nctagro
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of nct Class Mutagen Stocks Known lesion nctEY06883 P-element activity 1 -- nctST1 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nctST3 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nct5P3 ethyl methanesulfonate 0 Yes nct6F2 ethyl methanesulfonate 0 Yes nctA7 ethyl methanesulfonate 0 Yes nctagro 0 Yes nctE3 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 -- nctJ1 loss of function allele ethyl methanesulfonate 0 Yes nctJ2 loss of function allele ethyl methanesulfonate 0 Yes nctPIE-6 ethyl methanesulfonate 0 Yes nctR46 0 Yes nctSGE-10 ethyl methanesulfonate 0 Yes nctSGE-3 ethyl methanesulfonate 0 Yes nctSGE-8 ethyl methanesulfonate 0 Yes nctSGE-9 ethyl methanesulfonate 0 Yes nctSIE-22 ethyl methanesulfonate 0 Yes nctSIE-28 ethyl methanesulfonate 0 -- nctST2 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes nctST4 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nct Class Mutagen Stocks Known lesion nctJF02648 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nct+t7 in vitro construct - genomic fragment 0 Yes nct291-696.GMR in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes ncta.291-696.GMR in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes nctAct5C.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nctD329A.Y330A in vitro construct - amino acid replacement 0 Yes nctdsRNA.cFa in vitro construct - RNAi 0 Yes nctdsRNA.cHa in vitro construct - RNAi 0 Yes nctdsRNA.cIa in vitro construct - RNAi 0 Yes nctdsRNA.Scer\UAS.cLa in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nctGD252 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nctScer\UAS.cHa in vitro construct - regulatory fusion 0 -- nctT:Hsap\MYC in vitro construct - coding region fusion 0 -- nctαTub84B.PC in vitro construct - regulatory fusion 0 Yes nctΔ304-333 in vitro construct - deletion 0 Yes nctΔ607-637 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)96B (Hu et al., 2002) Df(3R)96ST (Zhang et al., 2005) Df(3R)Exel6200 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD252} NA P{TRiP.JF02648} NA P{UAS-nct.RNAi} NA characterization construct P{GMR-nct.RNAi} NA P{nct.7} NA P{αTub84B-nct.C} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}nctEY06883 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cytoskeleton organization inferred from mutant phenotype (Lopez-Schier and St. Johnston, 2002) lateral inhibition inferred from mutant phenotype (Zhang et al., 2005) membrane protein ectodomain proteolysis inferred from mutant phenotype (Chung and Struhl, 2001) Notch receptor processing inferred from genetic interaction with N AND inferred from mutant phenotype (Chung and Struhl, 2001) Notch signaling pathway inferred from mutant phenotype (Lopez-Schier and St. Johnston, 2001, Hu et al., 2002, Lopez-Schier and St. Johnston, 2002, Chung and Struhl, 2001) photoreceptor cell morphogenesis inferred from mutant phenotype (Zhang et al., 2005) R8 cell development inferred from mutant phenotype (Zhang et al., 2005) regulation of compound eye photoreceptor development inferred from mutant phenotype (Zhang et al., 2005)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
membrane protein ectodomain proteolysis non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) Notch signaling pathway non-traceable author statement (Yu, 2000.3.2) protein processing inferred from electronic annotation with InterPro:IPR008710 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR008710 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 2000.10.1) plasma membrane non-traceable author statement (Yu, 2000.3.2)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nct allele Gene References nctA7 N (Hu et al., 2002) nctagro btz (Lopez-Schier and St. Johnston, 2002) nctR46 N (Chung and Struhl, 2001) unspecified Psn (Mahoney et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67889 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0039234 DroID - A comprehensive database of gene and protein interactions. • FBgn0039234 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42964
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16997 Dere\GG11327 Dgri\GH19598 Dmoj\GI23992 Dper\GL13937 Dpse\GA20030 Dsec\GM26636 Dsim\GD21138 Dvir\GJ23626 Dwil\GK22490 Dyak\GE23523
Curated drosophilid orthologs	Dpse\GA20030
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004633-PA Aedes aegypti (mosquito) AGAP001734-PA Anopheles gambiae (mosquito) At3g52640.2 Arabidopsis thaliana (wall cress) 34433 Batrachochytrium dendrobatidis (chytrid fungus) 115392 Branchiostoma floridae (lancelet) XP_001898496.1 Brugia malayi (roundworm nematode) BGIBMGA007727-PA Bombyx mori (silk worm) ENSBTAP00000005677 Bos taurus (bovine) CBP05080 Caenorhabditis briggsae (nematode) CE34446 Caenorhabditis elegans (worm) ENSCAFP00000018497 Canis familiaris (dog) JA11191 Caenorhabditis japonica (nematode) CPIJ001617 Culex pipens (mosquito) ENSCPOP00000013448 Cavia porcellus (guinea pig) RP10569 Caenorhabditis remanei (nematode) 222879 Capitella spl (polychaete worm) DDB0237950 Dictyostelium discoideum (amoeba) 309169 Daphnia pulex (daphnia) ENSECAP00000007678 Equus caballus (horse) ENSGACP00000023450 Gymnopilus aculeatus (mushroom) ENSGALP00000014876 Gallus gallus (chicken) 190817 Hypsipyla robusta (cedar shoot borer) ENSP00000294785 Homo sapiens (human) 104663 Lottia gigantea (owl limpet) ENSMODP00000011863 Monodelphis domestica (gray opossum) ENSMMUP00000028781 Macaca mulatta (rhesus monkey) ENSMUSP00000003550 Mus musculus (house mouse) 244413 Nematostella vectensis (starlet anemone) XP_001601330.1 Nasonia vitripennis (wasp) ENSOANP00000005378 Ornithorynchus anatinus (platypus) ENSORLP00000003658 Oryzias latipes (japanese killifish) LOC_Os02g50360.1 Oryza sativa (asian rice) PHUM001527-PA Pediculus humanus (human body louse) PP10692 Pristionchus pacificus (nematode) 182421 Physcomitrella patens (moss) ENSPPYP00000000737 Pongo pygmaeus (orangutan) 198646 Populus trichocarpa (black cottonwood) ENSPTRP00000002601 Pan troglodytes (chimpanzee) ENSRNOP00000008150 Rattus norvegicus (norway rat) 5004509 Sorghum bicolor (sorghum) Smp_167240 Schistosoma mansoni (trematode parasite) XP_788243.2 Strongylocentrotus purpuratus (purple sea urchin) 55197 Trichoplax adhaerens (metazoa) ENSTNIP00000007242 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000025003 Takifugu rubripes (pufferfish Takifugu) 89137.m00118 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000056952 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12118 LH24484 FBgn0240701 FBgn0224467 FBgn0210723 FBgn0192590 FBgn0181489 JGI_V11_309169 FBgn0248340 FBgn0080026 FBgn0151542 FBgn0146715 FBgn0127062 FBgn0103628 FBgn0094016 CPIJ001617 BGIBMGA007727 ACYPI008285 GB11244 AGAP001734 AAEL011971 AAEL008666 AAEL006673 AAEL006671 AAEL004633 PB24941 PHUM368560 GLEAN_15142
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	27498 y1 v1; P{TRiP.JF02648}attP2 15979 y1 w67c23; P{EPgy2}nctEY06883/TM3, Sb1 Ser1
Genomic Clones ( 1 )
	BACR33F18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 191 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD36812
Other clones	DPiM_LD36812
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04630 GM07450 LD02341 LD05644 LD09213 LD09567 LD10229 LD11501 LD12685 LD16894 LD16896 LD17566 LD17574 LD21894 LD24375 LD24664 LD25715 LD27953 LD29770 LD33612 LD34159 LD38572 LD46252 LP07862 LP23003 LP23265 RE02736 RE04978 RE15456 RE17315 RE26188 RE26189 RE27579 RE29852 RE34482 RE37926 RE45472 RE46681 RE47443 RE47803 RE49074 RE53046 RE55750 RE60351 RE65695 RE72177 RE73807 SD05109 SD07928 SD08634 SD22402 SD24350
Other clones	1083 28114 29007 47838 57567 60431 61906 76352 76502 103443 144966 156253 178999 183097 211008 524252 533836 CK01.26D.H11 CK01.92B.D8 CK01.96A.C12 CK01.102C.D6 CK01.125C.C11 CK01.147A.D8 Dmel_neb_004717_0870_2637 EC33662 EC37270 EK025730 EK058806 EK072148 EK074742 EK096212 EK120148 EK122425 EK135723 EK137013 EK166520 EK175821 EK179601 EK185341 EK205536 EK206562 EK215506 EK252157 EK258828 EK260501 EK288361 EK291546 EN05030 EN10244 ESG017c.E21_H05 RP003019345 RP003020039 RP003027925 RP003027936 RP003031971 RP003034901 RP003043092 RP003059788 RP003060636 RP003068617 RP003081008 RP003081833 RP003086089 RP003090087 RP003092834 RP003119649 RP003124750 RP003127876 RP003130728 RP003133324 RP003148003 RP003150116 RP003153401 RP003160167 RP003161088 RP003174258 RP003176522 RP003199421 RP003205407 RP003215583 RP003226822 RP003233866 RP003238367 RP003245442 RP003259060 RP003260080 RP003260815 RP003266070 RP003284361 RP003291034 RP003293363 RP003307398 RP003321159 RP003323893 RP003327963 RP003332063 RP003336966 RP003358060 RP003370166 RP003385721 RP003397891 RP003404055 RP003412702 RP003418463 RP003421626 RP003422591 RP003423111 RP003432743 RP003444833 RP003458905 RP003459331 RP003461303 RP003468236 RP003479158 RP003485596 RP003495350 RP003504984 RP003507505 RP003510625 RP003522650 RP003538286 RP003551818 RP003556408 RP003558600 RP003561064 RP003565620 RP003566644 RP003567042 RP003575844 RP003578405 RP003585640 RP003589669 RP003606505 RP003647719 RP003652120 RP003654726 RP003656038
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039234 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42964
Antibody Information
	polyclonal (Stempfle et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: nicastrin CG7012 (FlyBase, 1996-)
Source for database merge of	Source for merge of: nct agro (Lopez-Schier and St. Johnston, 2002)
Additional comments	Source for identity of nicastrin CG7012 was sequence comparison (date:000915). (FlyBase, 1996-)
Other Comments
	dsRNA made from a cDNA library with primers directed against this gene (+674-1448) is used in S2 cells to assess the effect on Psn maturation. (Hu and Fortini, 2003) nct plays a role in the γ-secretase step of N processing, and is required for signaling from membrane-anchored activated N. nct also plays a role in stabilising the Psn gene product. (Hu et al., 2002) nct mutants and clones show N-like characteristics with defective cell fate specifications. (Hu et al., 2002) nct is required for the long term stability of Psn in the follicle cells of egg chambers. (Lopez-Schier and St. Johnston, 2002) nct is a novel neurogenic gene required cell autonomously for the transduction of the N signal in responding cells. The nct gene product is required for N signalling and acts specifically at the S3 cleavage step. nct and Psn have a novel function, independent of their role in N signaling, that relates to the organization of the spectrin cytoskeleton. (Lopez-Schier and St. Johnston, 2002) Partially processed N accumulates apically in nct and Psn mutant follicle cells, which also show a disrupted spectrin cytoskeleton. (Lopez-Schier and St. Johnston, 2002) The nct gene product is required for Psn-mediated transmembrane cleavage. (Chung and Struhl, 2001) Loss of nct activity blocks the accumulation of Psn gene product associated with the apical plasma membrane, abolishes Psn-dependent cleavage of the transmembrane domain of N and abrogates N signal transduction. (Chung and Struhl, 2001) Mutants block N signaling by affecting the processing of the receptor. (Lopez-Schier and St. Johnston, 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143040 NM_001170242 RefSeq (Proteins) • NP_651297 NP_001163713 DDBJ / EMBL / GenBank • AA246654 AAF56349 AAG11414 AAO25002 AAS65213 ACZ95007 AF240470 AI456701 AW943356 BT003245 Entrez Gene - A searchable database of RefSeq genes. • 42964 UniProtKB/Swiss-Prot • Q9VC27 UniProtKB/TrEMBL • E1JIV8 Q7KS07
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Nicastrin (IPR008710)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67889 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0039234 DroID - A comprehensive database of gene and protein interactions. • FBgn0039234 DRSC - Results from RNAi screens. • FBgn0039234 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039234 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7012-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039234 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42964 InParanoid A subset of ortholog calls from InParanoid. • AAEL004633-PA Aedes aegypti (mosquito) AGAP001734-PA Anopheles gambiae (mosquito) At3g52640.2 Arabidopsis thaliana (wall cress) 34433 Batrachochytrium dendrobatidis (chytrid fungus) 115392 Branchiostoma floridae (lancelet) XP_001898496.1 Brugia malayi (roundworm nematode) BGIBMGA007727-PA Bombyx mori (silk worm) ENSBTAP00000005677 Bos taurus (bovine) CBP05080 Caenorhabditis briggsae (nematode) CE34446 Caenorhabditis elegans (worm) ENSCAFP00000018497 Canis familiaris (dog) JA11191 Caenorhabditis japonica (nematode) CPIJ001617 Culex pipens (mosquito) ENSCPOP00000013448 Cavia porcellus (guinea pig) RP10569 Caenorhabditis remanei (nematode) 222879 Capitella spl (polychaete worm) DDB0237950 Dictyostelium discoideum (amoeba) 309169 Daphnia pulex (daphnia) ENSECAP00000007678 Equus caballus (horse) ENSGACP00000023450 Gymnopilus aculeatus (mushroom) ENSGALP00000014876 Gallus gallus (chicken) 190817 Hypsipyla robusta (cedar shoot borer) ENSP00000294785 Homo sapiens (human) 104663 Lottia gigantea (owl limpet) ENSMODP00000011863 Monodelphis domestica (gray opossum) ENSMMUP00000028781 Macaca mulatta (rhesus monkey) ENSMUSP00000003550 Mus musculus (house mouse) 244413 Nematostella vectensis (starlet anemone) XP_001601330.1 Nasonia vitripennis (wasp) ENSOANP00000005378 Ornithorynchus anatinus (platypus) ENSORLP00000003658 Oryzias latipes (japanese killifish) LOC_Os02g50360.1 Oryza sativa (asian rice) PHUM001527-PA Pediculus humanus (human body louse) PP10692 Pristionchus pacificus (nematode) 182421 Physcomitrella patens (moss) ENSPPYP00000000737 Pongo pygmaeus (orangutan) 198646 Populus trichocarpa (black cottonwood) ENSPTRP00000002601 Pan troglodytes (chimpanzee) ENSRNOP00000008150 Rattus norvegicus (norway rat) 5004509 Sorghum bicolor (sorghum) Smp_167240 Schistosoma mansoni (trematode parasite) XP_788243.2 Strongylocentrotus purpuratus (purple sea urchin) 55197 Trichoplax adhaerens (metazoa) ENSTNIP00000007242 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000025003 Takifugu rubripes (pufferfish Takifugu) 89137.m00118 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000056952 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/nicstrn1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42964 modMine - Data generated by the modENCODE project. • FBgn0039234 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV12118 LH24484 FBgn0240701 FBgn0224467 FBgn0210723 FBgn0192590 FBgn0181489 JGI_V11_309169 FBgn0248340 FBgn0080026 FBgn0151542 FBgn0146715 FBgn0127062 FBgn0103628 FBgn0094016 CPIJ001617 BGIBMGA007727 ACYPI008285 GB11244 AGAP001734 AAEL011971 AAEL008666 AAEL006673 AAEL006671 AAEL004633 PB24941 PHUM368560 GLEAN_15142
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	agro (Kopan and Goate, 2002, Lopez-Schier and St. Johnston, 2001, ) CG7012 (Nairz et al., 2002, Zhang et al., 2005, Perkins et al., 2009) dNct (Isoo et al., 2007) Nct (Hu and Fortini, 2003, Takasugi et al., 2003, Loewer et al., 2004, Stempfle et al., 2010) nct (Zhang et al., 2005, Mahoney et al., 2006, Guo et al., 2003, Ayroles et al., 2009, Nahvi et al., 2009, Vied and Kalderon, 2009, Saj et al., 2010) Nic (Hu et al., 2002) nic (Koelzer and Klein, 2006, Smith, 2001) S5A (Nairz et al., 2002) ST96B (Zhang et al., 2005)
Name Synonym	agoraphobic (Lopez-Schier and St. Johnston, 2001) dNicastrin (Arbeitman et al., 2002) nicastrin (Chung and Struhl, 2001, Lopez-Schier and St. Johnston, 2002, Zhang et al., 2005, Jutras et al., 2005, Smith, 2001, Zhou et al., 2008) Nicastrin (Hu et al., 2002)
Secondary FlyBase IDs
	FBgn0043964
References ( 46 )
Generate a list of	All references relating to this gene ( 46 )
List References by type	Research paper  ( 25 ) Supplementary material  ( 3 ) Review  ( 5 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 2 )
Recent research papers ( 2 )
	Saj et al., 2010, Dev. Cell 18(5): 862--876 A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network. [FBrf0210834] Stempfle et al., 2010, Mol. Cell. Biol. 30(13): 3165--3175 In vivo reconstitution of gamma-secretase in Drosophila results in substrate specificity. [FBrf0211009] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...