FlyBase Gene Report: Dmel\Nup358

General Information

Symbol

Dmel\Nup358

Species

D. melanogaster

Name

Nucleoporin 358kD

Annotation Symbol

CG11856

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039302

Gene Model Status

Stock Availability

6 publicly available

Gene Summary

E3 SUMO-protein ligase (By similarity). Component of the nuclear pore complex (NPC), a complex required for trafficking across the nuclear envelope (PubMed:17682050). Required for nuclear import of nuclear localization signal (NLS)-containing proteins in an importin alpha/importin beta-dependent manner, but also for the nuclear import of specific proteins such as phosphorylated Mad or the sesquiterpenoid juvenile hormone receptor Met as part of the juvenile hormone signal transduction pathway (PubMed:27979731, PubMed:17682050, PubMed:17682050). Plays a role in nuclear mRNA export by recruiting the mRNA transport complex composed of Nxt1 and sbr/Nxf1 to the NPC (PubMed:14729961). Essential during germline development for transposon silencing and piRNA biogenesis probably by regulating piwi localization to the nucleus (PubMed:29735528). During oogenesis, required to form granules that modulate the biogenesis of annulate lamellae containing nuclear pore complex components (PubMed:31626769). (UniProt, A0A0B4K7J2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

RanBP2

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:25,249,348..25,258,672 [+]

Recombination map

3-87

RefSeq locus

NT_033777 REGION:25249348..25258672

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:M9PBD9,UniProtKB:Q9U1H9,UniProtKB:Q9UKK6

(assigned by UniProt )

(Forler et al., 2004)

inferred from physical interaction with UniProtKB:P02828

(assigned by UniProt )

(He et al., 2017)

inferred from physical interaction with UniProtKB:Q9VKM1

(assigned by UniProt )

(Parikh et al., 2018)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

contributes_to GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

enables SUMO transferase activity

inferred from sequence or structural similarity with UniProtKB:P49792

(FlyBase Curators, 2008-)

Biological Process (13 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

involved_in germ cell development

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018)

involved_in juvenile hormone mediated signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

involved_in NLS-bearing protein import into nucleus

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

inferred from mutant phenotype

(Sabri et al., 2007)

involved_in nuclear pore complex assembly

inferred from mutant phenotype

(assigned by UniProt )

(Hampoelz et al., 2019)

acts_upstream_of piRNA metabolic process

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018)

involved_in positive regulation of cell population proliferation

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

involved_in positive regulation of protein localization to nucleus

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018, He et al., 2017)

involved_in positive regulation of RNA export from nucleus

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in protein import into nucleus

inferred from mutant phenotype

(Chen and Xu, 2010, Sabri et al., 2007)

involved_in regulation of protein localization

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in regulation of transposition

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018)

involved_in ventral cord development

inferred from high throughput mutant phenotype

(Bivik et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in annulate lamellae

inferred from direct assay

(assigned by UniProt )

(Hampoelz et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of nuclear pore

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX

SUMO TRANSFERASES

Protein Family (UniProt)

Belongs to the RanBP2 E3 ligase family. (A0A0B4K7J2)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX -: The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).
SUMO TRANSFERASES -: SUMO transferases catalyze the transfer of SUMO from either E2 or E3 enzyme to a substrate.

Protein Function (UniProtKB)

E3 SUMO-protein ligase (By similarity). Component of the nuclear pore complex (NPC), a complex required for trafficking across the nuclear envelope (PubMed:17682050). Required for nuclear import of nuclear localization signal (NLS)-containing proteins in an importin alpha/importin beta-dependent manner, but also for the nuclear import of specific proteins such as phosphorylated Mad or the sesquiterpenoid juvenile hormone receptor Met as part of the juvenile hormone signal transduction pathway (PubMed:27979731, PubMed:17682050, PubMed:17682050). Plays a role in nuclear mRNA export by recruiting the mRNA transport complex composed of Nxt1 and sbr/Nxf1 to the NPC (PubMed:14729961). Essential during germline development for transposon silencing and piRNA biogenesis probably by regulating piwi localization to the nucleus (PubMed:29735528). During oogenesis, required to form granules that modulate the biogenesis of annulate lamellae containing nuclear pore complex components (PubMed:31626769).

(UniProt, A0A0B4K7J2)

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Nup358 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4K7J2)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0084813

8484

2695

FBtr0304692

8553

2718

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0084188

296.4

2695

5.96

FBpp0293235

298.9

2718

5.98

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Part of the nuclear pore complex (By similarity). Forms a complex with Nxt1, sbr/Nxf1 and RanGAP (PubMed:14729961). Interacts (via TPR repeats) with Hsp83; the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731). Interacts (via N-terminus) with piwi (PubMed:29735528).

(UniProt, A0A0B4K7J2)

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.

(UniProt, A0A0B4K7J2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

A0A0B4K7J2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup358 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nup358_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in annulate lamellae

inferred from direct assay

(assigned by UniProt )

(Hampoelz et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Nup358 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nup358

View exonic expression by tissue for Dmel\Nup358

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43041/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039302

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039302

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039302

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11856

Flygut - An atlas of the Drosophila adult midgut

FBgn0039302

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nup358

Transgenic constructs containing regulatory region of Nup358

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)FDD-0089346

(Bivik et al., 2015)

Df(3R)FDD-0247625

(DrosDel Project, 2008.11.14)

Df(3R)FDD-0247627

(Bivik et al., 2015, DrosDel Project, 2008.11.14)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

lethal - all die during P-stage

Nup358^A

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Nup358^emGFP

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nup358^GD7405

viable, with Scer\GAL4^tin.CΔ4

Nup358^GD7405

Sterility

Allele

Nup358^emGFP

Other Phenotypes

Allele

abnormal developmental rate | P-stage | recessive

Nup358^A

abnormal neuroanatomy

some die during pupal stage, with Scer\GAL4^pnr-MD237

Nup358^GD7405

visible, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Phenotype manifest in

Allele

annulate lamellae | oogenesis, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

apterous-expressing neuron of the lateral cluster of the thorax

apterous-expressing neuron of the lateral cluster of the thorax Ap4

egg chamber, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Nup358^GD7405

tormogen cell, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

9 of 15

Yes

Yes

9 of 15

Yes

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

6 of 15

No

Yes

5 of 15

No

Yes

2 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

6 of 15

No

Yes

1 of 15

No

No

Rattus norvegicus (Norway rat) (3)

5 of 13

Yes

Yes

4 of 13

No

Yes

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (3)

5 of 12

Yes

Yes

4 of 12

No

Yes

1 of 12

No

No

Danio rerio (Zebrafish) (2)

4 of 15

Yes

Yes

Drer\si:ch1073-55a19.2

3 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

5 of 15

Yes

Yes

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (5)

2 of 9

Yes

Yes

2 of 9

Yes

Yes

2 of 9

Yes

Yes

1 of 9

No

No

1 of 9

No

No

Saccharomyces cerevisiae (Brewer's yeast) (3)

7 of 15

Yes

Yes

4 of 15

No

Yes

2 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (3)

4 of 12

Yes

Yes

1 of 12

No

No

1 of 12

No

No

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RANBP2; RAN binding protein 2

9 of 15

ENCEPHALOPATHY, ACUTE, INFECTION-INDUCED, SUSCEPTIBILITY TO, 3; IIAE3

RGPD3; RANBP2 like and GRIP domain containing 3

9 of 15

RGPD1; RANBP2 like and GRIP domain containing 1

8 of 15

RGPD2; RANBP2 like and GRIP domain containing 2

8 of 15

RGPD4; RANBP2 like and GRIP domain containing 4

8 of 15

RGPD6; RANBP2 like and GRIP domain containing 6

8 of 15

RGPD8; RANBP2 like and GRIP domain containing 8

8 of 15

RANBP1; RAN binding protein 1

6 of 15

RGPD5; RANBP2 like and GRIP domain containing 5

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - CG3008

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - CG9467

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fukuyama et al., 2012)

anti bait coimmunoprecipitation, western blot, proximity-dependent biotin identification, Identification by mass spectrometry

(Mazina et al., 2020)

Nup358 - Fs(2)Ket

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - GAPcenA

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(He et al., 2017)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2007)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based, pull down, molecular weight estimation by staining

(Zhao et al., 2019, Guruharsha et al., 2011)

Nup358 - Pi3K68D

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - Pih1D1

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nup358 - RanGAP

pull down, western blot, anti bait coimmunoprecipitation, molecular weight estimation by staining, experimental knowledge based

(Chen et al., 2021, Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

(Gawlinski et al., 2007)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Parikh et al., 2018)

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, western blot, experimental knowledge based, pull down, molecular weight estimation by staining

(Zhao et al., 2019, Guruharsha et al., 2011, Kozlova et al., 2006)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Chen et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Part of the nuclear pore complex (By similarity). Forms a complex with Nxt1, sbr/Nxf1 and RanGAP (PubMed:14729961). Interacts (via TPR repeats) with Hsp83; the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731). Interacts (via N-terminus) with piwi (PubMed:29735528).

(UniProt, A0A0B4K7J2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67960

DroID - A comprehensive database of gene and protein interactions.

FBgn0039302

MIST (protein-protein) - An integrated Molecular Interaction Database

Nup358

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0039302

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-87

Cytogenetic map

Sequence location

3R:25,249,348..25,258,672 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96C8-96C8

Limits computationally determined from genome sequence between P{lacW}OstStt3^j2D9 and P{PZ}Aats-gln⁰⁵⁴⁶¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-87

(FlyBase, 2016)

3-85.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

62684

w¹¹¹⁸; PBac{IT.GAL4}Nup358^0175-G4/TM6B, Tb¹

33003

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00803}attP2

34967

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00865}attP2

59396

w¹¹¹⁸; P{FMRFa-EGFP.Tv}3, P{UAS-myr-mRFP}2, Nup358^12A002/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

v38583

w¹¹¹⁸; P{GD7405}v38583

v38581

w¹¹¹⁸; P{GD7405}v38581/CyO

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (68)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD43045

Other clones

LD24888

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039302

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039302

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43041

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Arnaoutov et al., 2020)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43041

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4K7J2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A0A0B4K7J2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4K7J2

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039302

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43041/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039302

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nup358_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039302

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039302

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11856

Flygut - An atlas of the Drosophila adult midgut

FBgn0039302

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0039302

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43041

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002881

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11856

MARRVEL_MODEL - MARRVEL (model organism gene)

43041

modMine - A data warehouse for the modENCODE project

FBgn0039302

Linkouts

BioGRID - A database of protein and genetic interactions.

67960

DroID - A comprehensive database of gene and protein interactions.

FBgn0039302

DRSC - Results frm RNAi screens

FBgn0039302

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039302

FlyMine - An integrated database for Drosophila genomics

FBgn0039302

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (protein-protein) - An integrated Molecular Interaction Database

Nup358

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG11856

(Kolkhof et al., 2017, Schwartz et al., 2016, Hosono et al., 2015, Ni et al., 2011.7.19, Müller et al., 2010, Ni et al., 2010.12.1, Hughes et al., 2008, Przewloka et al., 2007, Sabri et al., 2007, Cermelli et al., 2006, Giot et al., 2003)

NUP358

(Joyce et al., 2012)

Q9VBU7_DROME

(Fukuyama et al., 2012)

RanBP2

(Bernard et al., 2021, Hughes et al., 2008, Forler et al., 2004)

dmRanBP

(Chen et al., 2021)

nup358

(Munafò et al., 2021, Chen and Xu, 2010, Sabri et al., 2007)

ranbp2

(Chen et al., 2021)

Name Synonyms

Nucleoporin 358

Nucleoporin 358kD

Nup358

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (81)