FlyBase Gene Report: Dmel\Nup358

NUCLEAR PORE COMPLEX -: The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).
SUMO TRANSFERASES -: SUMO transferases catalyze the transfer of SUMO from either E2 or E3 enzyme to a substrate.

Protein Function (UniProtKB)

E3 SUMO-protein ligase (By similarity). Component of the nuclear pore complex (NPC), a complex required for trafficking across the nuclear envelope (PubMed:17682050). Required for nuclear import of nuclear localization signal (NLS)-containing proteins in an importin alpha/importin beta-dependent manner, but also for the nuclear import of specific proteins such as phosphorylated Mad or the sesquiterpenoid juvenile hormone receptor Met as part of the juvenile hormone signal transduction pathway (PubMed:27979731, PubMed:17682050, PubMed:17682050). Plays a role in nuclear mRNA export by recruiting the mRNA transport complex composed of Nxt1 and sbr/Nxf1 to the NPC (PubMed:14729961). Essential during germline development for transposon silencing and piRNA biogenesis probably by regulating piwi localization to the nucleus (PubMed:29735528).

(UniProt, A0A0B4K7J2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Nup358 or the JBrowse view of Dmel\Nup358 for information on other features

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Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

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end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

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Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nup358-RA

FBtr0084813

NM_143104

8484

2695

Nup358-RB

FBtr0304692

NM_001260373

8553

2718

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Nup358-PA

FBpp0084188

296.4

2695

5.96

NP_651361

AAF56430

Nup358-PB

FBpp0293235

298.9

2718

5.98

NP_001247302

AFH06619

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Forms a complex with Nxt1, sbr/Nxf1 and RanGAP (PubMed:14729961). Interacts (via TPR repeats) with Hsp83; the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731). Interacts (via N-terminus) with piwi (PubMed:29735528).

(UniProt, A0A0B4K7J2)

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.

(UniProt, A0A0B4K7J2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

A0A0B4K7J2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup358 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nup358_1

Linkouts

Gene Ontology (20 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

protein binding

inferred from physical interaction with UniProtKB:Q9VKM1

(assigned by UniProt )

(Parikh et al., 2018)

inferred from physical interaction with UniProtKB:P02828

(assigned by UniProt )

(He et al., 2017)

inferred from physical interaction with UniProtKB:M9PBD9,UniProtKB:Q9U1H9,UniProtKB:Q9UKK6

(assigned by UniProt )

(Forler et al., 2004)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

contributes_to GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

Ran GTPase binding

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

SUMO transferase activity

inferred from sequence or structural similarity with UniProtKB:P49792

(FlyBase Curators, 2008--)

Biological Process (12 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

germ cell development