FlyBase Gene Report: Dmel\Nup358

General Information

Symbol

Dmel\Nup358

Species

D. melanogaster

Name

Nucleoporin 358kD

Annotation Symbol

CG11856

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039302

Gene Model Status

Stock Availability

6 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

RanBP2

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:25,249,348..25,258,672 [+]

Recombination map

3-87

RefSeq locus

NT_033777 REGION:25249348..25258672

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VKM1

(assigned by UniProt )

(Parikh et al., 2018)

inferred from physical interaction with UniProtKB:P02828

(assigned by UniProt )

(He et al., 2017)

inferred from physical interaction with UniProtKB:M9PBD9,UniProtKB:Q9U1H9,UniProtKB:Q9UKK6

(assigned by UniProt )

(Forler et al., 2004)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

contributes_to GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

enables small GTPase binding

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

enables SUMO transferase activity

inferred from sequence or structural similarity with UniProtKB:P49792

(FlyBase Curators, 2008--)

Biological Process (12 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in germ cell development

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018)

involved_in juvenile hormone mediated signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

involved_in NLS-bearing protein import into nucleus

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

inferred from mutant phenotype

(Sabri et al., 2007)

involved_in nuclear pore complex assembly

inferred from mutant phenotype

(assigned by UniProt )

(Hampoelz et al., 2019)

involved_in positive regulation of cell population proliferation

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(assigned by UniProt )

(He et al., 2017)

involved_in positive regulation of protein localization to nucleus

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018, He et al., 2017)

involved_in positive regulation of RNA export from nucleus

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in protein import into nucleus

inferred from mutant phenotype

(Chen and Xu, 2010, Sabri et al., 2007)

involved_in regulation of cellular protein localization

inferred from mutant phenotype

(assigned by UniProt )

(Forler et al., 2004)

involved_in regulation of transposition

inferred from mutant phenotype

(assigned by UniProt )

(Parikh et al., 2018)

involved_in ventral cord development

inferred from high throughput mutant phenotype

(Bivik et al., 2015)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in annulate lamellae

inferred from direct assay

(assigned by UniProt )

(Hampoelz et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of nuclear pore

inferred from biological aspect of ancestor with PANTHER:PTN000566604

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX

SUMO TRANSFERASES

Protein Family (UniProt)

Belongs to the RanBP2 E3 ligase family. (A0A0B4K7J2)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX -: The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).
SUMO TRANSFERASES -: SUMO transferases catalyze the transfer of SUMO from either E2 or E3 enzyme to a substrate.

Protein Function (UniProtKB)

E3 SUMO-protein ligase (By similarity). Component of the nuclear pore complex (NPC), a complex required for trafficking across the nuclear envelope (PubMed:17682050). Required for nuclear import of nuclear localization signal (NLS)-containing proteins in an importin alpha/importin beta-dependent manner, but also for the nuclear import of specific proteins such as phosphorylated Mad or the sesquiterpenoid juvenile hormone receptor Met as part of the juvenile hormone signal transduction pathway (PubMed:27979731, PubMed:17682050, PubMed:17682050). Plays a role in nuclear mRNA export by recruiting the mRNA transport complex composed of Nxt1 and sbr/Nxf1 to the NPC (PubMed:14729961). Essential during germline development for transposon silencing and piRNA biogenesis probably by regulating piwi localization to the nucleus (PubMed:29735528). During oogenesis, required to form granules that modulate the biogenesis of annulate lamellae containing nuclear pore complex components (PubMed:31626769).

(UniProt, A0A0B4K7J2)

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Nup358 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0084813

8484

2695

FBtr0304692

8553

2718

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0084188

296.4

2695

5.96

FBpp0293235

298.9

2718

5.98

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Part of the nuclear pore complex (By similarity). Forms a complex with Nxt1, sbr/Nxf1 and RanGAP (PubMed:14729961). Interacts (via TPR repeats) with Hsp83; the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731). Interacts (via N-terminus) with piwi (PubMed:29735528).

(UniProt, A0A0B4K7J2)

Domain

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited.

(UniProt, A0A0B4K7J2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

A0A0B4K7J2

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup358 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nup358_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in annulate lamellae

inferred from direct assay

(assigned by UniProt )

(Hampoelz et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Nup358 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nup358

View exonic expression by tissue for Dmel\Nup358

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0039302

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11856-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11856

Flygut - An atlas of the Drosophila adult midgut

FBgn0039302

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nup358

Transgenic constructs containing regulatory region of Nup358

Deletions and Duplications ( 3 )

Disrupted in

Df(3R)FDD-0089346

(Bivik et al., 2015)

Df(3R)FDD-0247625

(DrosDel Project, 2008.11.14)

Df(3R)FDD-0247627

(Bivik et al., 2015, DrosDel Project, 2008.11.14)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Nup358^emGFP

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nup358^GD7405

viable, with Scer\GAL4^tin.CΔ4

Nup358^GD7405

Sterility

Allele

Nup358^emGFP

Other Phenotypes

Allele

neuroanatomy defective

some die during pupal stage, with Scer\GAL4^pnr-MD237

Nup358^GD7405

visible, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Phenotype manifest in

Allele

annulate lamellae | oogenesis, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

apterous-expressing neuron of the lateral cluster of the thorax

apterous-expressing neuron of the lateral cluster of the thorax Ap4

egg chamber, with Scer\GAL4^VP16.nos.UTR

Nup358^HMS00865

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Nup358^GD7405

tormogen cell, with Scer\GAL4^pnr-MD237

Nup358^GD7405

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

9 of 15

Yes

Yes

9 of 15

Yes

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

8 of 15

No

Yes

6 of 15

No

Yes

5 of 15

No

Yes

2 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

8 of 15

Yes

Yes

6 of 15

No

Yes

1 of 15

No

No

Rattus norvegicus (Norway rat) (3)

5 of 13

Yes

Yes

4 of 13

No

Yes

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (3)

5 of 12

Yes

Yes

4 of 12

No

Yes

1 of 12

No

No

Danio rerio (Zebrafish) (2)

4 of 15

Yes

Yes

Drer\si:ch1073-55a19.2

3 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

5 of 15

Yes

Yes

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (5)

2 of 9

Yes

Yes

2 of 9

Yes

Yes

2 of 9

Yes

Yes

1 of 9

No

No

1 of 9

No

No

Saccharomyces cerevisiae (Brewer's yeast) (3)

7 of 15

Yes

Yes

4 of 15

No

Yes

2 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (3)

4 of 12

Yes

Yes

1 of 12

No

No

1 of 12

No

No

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900FS )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00011375

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501HD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY002636

Glossina morsitans

Tsetse fly

Gmor\GMOY002763

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_02494

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10673

Mayetiola destructor

Hessian fly

Mdes\Mdes006428

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009884

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009457

Anopheles darlingi

American malaria mosquito

Adar\ADAC004547

Anopheles gambiae

Malaria mosquito

Agam\AGAP002982

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ007518

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01A6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007513

Danaus plexippus

Monarch butterfly

Dple\DPOGS204234-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015164

Apis florea

Little honeybee

Aflo\2151263028

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00257a

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG006437

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG003637

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM477850

Rhodnius prolixus

Kissing bug

Rpro\RPRC003552

Cimex lectularius

Bed bug

Clec\CLEC001769

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001796

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01831

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0052 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR006742

Ixodes scapularis

Black-legged tick

Isca\ISCW015975

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58843.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58844.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM83186.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur16g02910

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304059

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0BGL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_000546gn

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pdpi(cyclophilin)L8

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pdpi(cyclophilin)L5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ranbp2L_4

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015986gn

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pdpi(cyclophilin)L3

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ranbp2L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hypp_2426

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019830gn

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pdpi(cyclophilin)L7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ranbp2L_3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ranbp2_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ranbp2L_2

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000013303

Ciona intestinalis

Vase tunicate

Cint\LOC100177752

Ciona intestinalis

Vase tunicate

Cint\LOC100187126

Ciona intestinalis

Vase tunicate

Cint\LOC100183452

Ciona intestinalis

Vase tunicate

Cint\LOC100180164

Ciona intestinalis

Vase tunicate

Cint\LOC100182981

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000021983

Ciona intestinalis

Vase tunicate

Cint\LOC100183566

Ciona intestinalis

Vase tunicate

Cint\LOC100181997

Ciona intestinalis

Vase tunicate

Cint\LOC101242267

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\LOC100859731

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000028600

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000001269

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Gallus gallus

Domestic chicken

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

2 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RANBP2; RAN binding protein 2

9 of 15

ENCEPHALOPATHY, ACUTE, INFECTION-INDUCED, SUSCEPTIBILITY TO, 3; IIAE3

RGPD3; RANBP2 like and GRIP domain containing 3

9 of 15

RGPD1; RANBP2 like and GRIP domain containing 1

8 of 15

RGPD2; RANBP2 like and GRIP domain containing 2

8 of 15

RGPD4; RANBP2 like and GRIP domain containing 4

8 of 15

RGPD6; RANBP2 like and GRIP domain containing 6

8 of 15

RGPD8; RANBP2 like and GRIP domain containing 8

8 of 15

RANBP1; RAN binding protein 1

6 of 15

RGPD5; RANBP2 like and GRIP domain containing 5

5 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - CG2064

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - CG3008

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - CG9467

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Fukuyama et al., 2012)

proximity-dependent biotin identification, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Mazina et al., 2020)

Nup358 - Fs(2)Ket

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - GAPcenA

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot

(He et al., 2017)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2007)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based, pull down, molecular weight estimation by staining

(Zhao et al., 2019, Guruharsha et al., 2011)

Nup358 - Pi3K68D

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - Pih1D1

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nup358 - RanGAP

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

(Gawlinski et al., 2007)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Belozerov et al., 2012)

anti bait coimmunoprecipitation, western blot, pull down, anti tag western blot

(Parikh et al., 2018)

experimental knowledge based

(Guruharsha et al., 2011)

Nup358 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

experimental knowledge based, pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, western blot

(Zhao et al., 2019, Guruharsha et al., 2011, Kozlova et al., 2006)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Part of the nuclear pore complex (By similarity). Forms a complex with Nxt1, sbr/Nxf1 and RanGAP (PubMed:14729961). Interacts (via TPR repeats) with Hsp83; the interaction is required for the nuclear import of the sesquiterpenoid juvenile hormone receptor Met (PubMed:27979731). Interacts (via N-terminus) with piwi (PubMed:29735528).

(UniProt, A0A0B4K7J2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67960

DroID - A comprehensive database of gene and protein interactions.

FBgn0039302

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43041

MIST (protein-protein) - An integrated Molecular Interaction Database

Nup358

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-87

Cytogenetic map

Sequence location

3R:25,249,348..25,258,672 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96C8-96C8

Limits computationally determined from genome sequence between P{lacW}OstStt3^j2D9 and P{PZ}Aats-gln⁰⁵⁴⁶¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-87

(FlyBase, 2016)

3-85.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

62684

w¹¹¹⁸; PBac{IT.GAL4}Nup358^0175-G4/TM6B, Tb¹

33003

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00803}attP2

34967

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00865}attP2

59396

w¹¹¹⁸; P{FMRFa-EGFP.Tv}3, P{UAS-myr-mRFP}2, Nup358^12A002/TM6B, P{Dfd-EYFP}3, Sb¹ Tb¹ ca¹

v38583

w¹¹¹⁸; P{GD7405}v38583

v38581

w¹¹¹⁸; P{GD7405}v38581/CyO

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (68)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD43045

Other clones

LD24888

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039302

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039302

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43041

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Arnaoutov et al., 2020)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43041

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A0A0B4K7J2

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039302

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nup358_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG11856

Flygut - An atlas of the Drosophila adult midgut

FBgn0039302

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43041

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002881

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG11856

modMine - A data warehouse for the modENCODE project

FBgn0039302

Linkouts

BioGRID - A database of protein and genetic interactions.

67960

DroID - A comprehensive database of gene and protein interactions.

FBgn0039302

DRSC - Results frm RNAi screens

FBgn0039302

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0039302

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG11856-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039302

FlyMine - An integrated database for Drosophila genomics

FBgn0039302

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43041

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (protein-protein) - An integrated Molecular Interaction Database

Nup358

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG11856

(Kolkhof et al., 2017, Schwartz et al., 2016, Hosono et al., 2015, Ni et al., 2011.7.19, Müller et al., 2010, Ni et al., 2010.12.1, Hughes et al., 2008, Przewloka et al., 2007, Sabri et al., 2007, Cermelli et al., 2006, Giot et al., 2003)

NUP358

(Joyce et al., 2012)

Q9VBU7_DROME

(Fukuyama et al., 2012)

RanBP2

(Hughes et al., 2008, Forler et al., 2004)

nup358

(Chen and Xu, 2010, Sabri et al., 2007)

Name Synonyms

Nucleoporin 358

Nucleoporin 358kD

Nup358

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (74)