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Gene Dmel\TwdlB

General Information
Symbol	Dmel\TwdlB	Species	D. melanogaster
Name	TweedleB	Annotation symbol	CG6478
Feature type	protein_coding_gene	FlyBase ID	FBgn0039436
Gene Model Status	Current	Stock availability	1 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	97C2-97C2	Sequence location	3R:22,448,173..22,449,270 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TweedleB is referred to in FlyBase by the symbol Dmel\TwdlB (CG6478, FBgn0039436). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: structural constituent of chitin-based cuticle. Based on sequence similarity, it is predicted to be involved in the biological process: chitin-based cuticle development; body morphogenesis. One allele is reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Domain of unknown function DUF243. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1626765_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TwdlB. Gene sequence location is 3R:22448173..22449270.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References FlyBase Curators, 2010-, Gene Ontology (GO) annotation review.
FB2012_01	References Cornman, 2010, PLoS ONE 5(9): e12536 Sequence features HFA16050 BKN31755
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 97C2-97C2   Limits computationally determined from genome sequence between P{lacW}scribj7B3 and P{lacW}His2AvL1602
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TwdlB for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TwdlB-RA FBtr0085057  NM_143229   1030   286
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TwdlB-PA   FBpp0084429   28.6   286   6.27     NP_651486   AAF56602
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56602   AI260102   AY071163   AY071379   AY084171 AAL89909   AY231936   BI164806   BI167702   BI167975   BI171108   BI171897   BI172457   BI173541   BI212397   BI212994   BI230415   BI230857   BI239968   BI240376   BI242525   BI243269   BI358350   BI358487   BI363297   BI365022   BI367251   BI367550   BI371180   BI371721   BI375157   BI484733   BI578760   BI579057   BT001610   BT001696   BT003571   BT044113   BT044324   CO278066   CO292498   EC212449   EC233112   FD585658   FD585784   FD585906   FD585910   FD586301   FD586302   FD586315   GH739951   GH740235   GH740400   GH740479   GH744150   GH745257   GH745989   GH746097   GH746630   GH761664   GH762325   GH766139   GH766620   GH767057   GH768032   GH768718   GH768910   GH769540
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SXS1 Q9VBE0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0039436    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0039436 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0039436 expression level Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr    46823 embryo 18-20hr    180766 embryo 20-22hr    14422 embryo 22-24hr    21702 larva L1    489 larva L2    8836 larva L3 12hr old    30458 larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (163) embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr  (362) embryo 10-12hr  (237) embryo 12-14hr  (141) embryo 14-16hr  (3737) embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  (14422) embryo 22-24hr  (21702) larva L1  (489) larva L2  (8836) larva L3 12hr old  (30458) larva L3 puffstage 1-2  (173) larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP  (287) pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr  (3737) embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  (14422) embryo 22-24hr  (21702) larva L1    489 larva L2  (8836) larva L3 12hr old  (30458) larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  (14422) embryo 22-24hr  (21702) larva L1    489 larva L2    8836 larva L3 12hr old  (30458) larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr    46823 embryo 18-20hr    180766 embryo 20-22hr    14422 embryo 22-24hr    21702 larva L1    489 larva L2    8836 larva L3 12hr old    30458 larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0039436 expression level Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr    46823 embryo 18-20hr    180766 embryo 20-22hr    14422 embryo 22-24hr    21702 larva L1    489 larva L2    8836 larva L3 12hr old    30458 larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (163) embryo 02-04hr  (163) embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr  (362) embryo 10-12hr  (237) embryo 12-14hr  141 embryo 14-16hr  (3737) embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  (14422) embryo 22-24hr  (21702) larva L1  (489) larva L2  (8836) larva L3 12hr old  (30458) larva L3 puffstage 1-2  (173) larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP  (287) pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr  (3737) embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  (14422) embryo 22-24hr  (21702) larva L1    489 larva L2  (8836) larva L3 12hr old  (30458) larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr  (46823) embryo 18-20hr  (180766) embryo 20-22hr  14422 embryo 22-24hr  (21702) larva L1    489 larva L2    8836 larva L3 12hr old  (30458) larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    163 embryo 02-04hr    163 embryo 04-06hr    44 embryo 06-08hr    48 embryo 08-10hr    362 embryo 10-12hr    237 embryo 12-14hr    141 embryo 14-16hr    3737 embryo 16-18hr    46823 embryo 18-20hr    180766 embryo 20-22hr    14422 embryo 22-24hr    21702 larva L1    489 larva L2    8836 larva L3 12hr old    30458 larva L3 puffstage 1-2    173 larva L3 puffstage 3-6    28 larva L3 puffstage 7-9    0 white prepupae new    0 white prepupae 12hr    16 white prepupae 24hr    5 pupae 2d postWPP    287 pupae 3d postWPP    1 pupae 4d postWPP    10 adult male 01day    2 adult male 05day    5 adult male 30day    2 adult female 01day    0 adult female 05day    4 adult female 30day    1 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0039436    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0039436 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0039436 expression level Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0039436 expression level Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.45 Larval Midgut    4.4 Larval Hindgut    3.1 Larval Malpighian Tubules    5.3 Larval Fat Body    4.7 Larval Salivary Gland    3.7 Larval Trachea    2.225 Larval Carcass    2.825 Adult Head    2.9 Adult Eye    4.9 Adult Brain    2.4 Adult Thoracic-Abdominal Ganglion    4 Adult Crop    9.2 Adult Midgut    5.3 Adult Hindgut    7.1 Adult Malpighian Tubules    5.5 Adult Fat Body    2 Adult Salivary Gland    9.4 Adult Heart    4.025 Adult VirginFemale Spermatheca    2.2 Adult InseminatedFemale Spermatheca    2.9 Adult Ovary    3.5 Adult Testis    3 Adult Male Accessory Gland    7.8 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.9   NA  Eye    4.9   NA  Brain    2.4   3.45  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    4   NA  Crop    9.2   4.4  Midgut    5.3   3.1  Hindgut    7.1   5.3  Malpighian Tubules    5.5   4.7  Fat Body    2   3.7  Salivary Gland    9.4   NA  Heart    4.025   2.225  Trachea    NA   NA  VirginFemale Spermatheca    2.2   NA  InseminatedFemale Spermatheca    2.9   NA  Ovary    3.5   NA  Testis    3   NA  Male Accessory Gland    7.8   2.825  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_12
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039436 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6478-RA;CG6447-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TwdlB Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of TwdlB Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TwdlB Class Mutagen Stocks Known lesion TwdlBGD17296 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)ths238 (Kadam et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
structural constituent of chitin-based cuticle inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Biological Process
CV term Evidence References
body morphogenesis inferred from sequence or structural similarity with TwdlD (Curators, 2008--) chitin-based cuticle development inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Cellular Component
CV term Evidence References
proteinaceous extracellular matrix inferred from sequence or structural similarity with TwdlD (Curators, 2008--)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TwdlB allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68097 DroID - A comprehensive database of gene and protein interactions. • FBgn0039436 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43202
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18576 Dere\GG12182 Dper\GL24442 Dsec\GM10178 Dyak\GE10626
Curated drosophilid orthologs
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0228479 FBgn0228478 FBgn0215968 FBgn0215969 FBgn0215329 FBgn0201771 FBgn0201770 FBgn0201391 FBgn0201388 FBgn0192730 FBgn0189672 FBgn0165281 FBgn0165128 FBgn0165129 FBgn0248511 FBgn0247988 FBgn0247987 FBgn0162031 FBgn0162030 FBgn0160689 FBgn0147458 FBgn0147457 FBgn0145604 FBgn0039437 FBgn0039434 FBgn0126346 FBgn0126191 FBgn0104473 FBgn0095594 FBgn0095593
Stocks & Reagents
Stocks Listed in FlyBase ( 1 )
VDRC	v52320 w1118; P{GD17296}v52320/TM3
Genomic Clones ( 2 )
	BACR08N03 BACR13P17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 66 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD41821 RE20092 RE31819 RE40518 RE40851 RE59605
Other clones	DPiM_RE40518 FI02846 FI07246 yak-em_CG6478
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP03787 RE04553 RE08144 RE08502 RE12787 RE13790 RE14057 RE14953 RE16114 RE16477 RE17482 RE18287 RE36606 RE37211 RE39885 RE45096 RE45263 RE48070 RE50240 RE52969 RE53342 RE58026 RE58668 RE62896 RE67878 RE72674 RE73030
Other clones	17002 71804 EK128639 EK200324 RM01030 RM01130 RM01230 RM01330 RM01530 RM01630 RM01730 RP001001869 RP001008541 RP001013055 RP001016536 RP001016931 RP001018985 RP001021943 RP001025224 RP001029741 RP001037724 RP001040466 RP001042322 RP001046126 RP001048238 RP001048548 RP001052814 RP001056332 RP001056798
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039436 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43202
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: TwdlB CG6478 (Guan, 2006)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143229 RefSeq (Proteins) • NP_651486 DDBJ / EMBL / GenBank • AAF56602 AAL89909 AY084171 BI243269 Entrez Gene - A searchable database of RefSeq genes. • 43202 UniProtKB/TrEMBL • Q8SXS1 Q9VBE0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68097 DroID - A comprehensive database of gene and protein interactions. • FBgn0039436 DRSC - Results from RNAi screens. • FBgn0039436 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039436 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6478-RA;CG6447-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039436 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43202 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43202 modMine - Data generated by the modENCODE project. • FBgn0039436 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0228479 FBgn0228478 FBgn0215968 FBgn0215969 FBgn0215329 FBgn0201771 FBgn0201770 FBgn0201391 FBgn0201388 FBgn0192730 FBgn0189672 FBgn0165281 FBgn0165128 FBgn0165129 FBgn0248511 FBgn0247988 FBgn0247987 FBgn0162031 FBgn0162030 FBgn0160689 FBgn0147458 FBgn0147457 FBgn0145604 FBgn0039437 FBgn0039434 FBgn0126346 FBgn0126191 FBgn0104473 FBgn0095594 FBgn0095593
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG6478 (Guan, 2006, Guan et al., 2006, Blanco et al., 2008) TwdlB (Guan, 2006, Guan et al., 2006, Kadam et al., 2009)
Name Synonym	CG6478   TweedleB (Cornman, 2010)
Secondary FlyBase IDs
References ( 11 )
Research paper	Cornman, 2010, PLoS ONE 5(9): e12536 The Distribution of GYR- and YLP-Like Motifs in Drosophila Suggests a General Role in Cuticle Assembly and Other Protein-Protein Interactions. [FBrf0211774] Kadam et al., 2009, Development 136(5): 739--747 FGF ligands in Drosophila have distinct activities required to support cell migration and differentiation. [FBrf0206790] Blanco et al., 2008, Genome Biol. 9(9): R134 Conserved chromosomal clustering of genes governed by chromatin regulators in Drosophila. [FBrf0207036] Guan et al., 2006, Proc. Natl. Acad. Sci. USA 103(45): 16794--16799 Mutation of TweedleD, a member of an unconventional cuticle protein family, alters body shape in Drosophila. [FBrf0195309] Gonzalez-Aguero et al., 2005, Gene 345(2): 213--224 Identification of genes expressed during Drosophila melanogaster gastrulation by using subtractive hybridization. [FBrf0187577]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Guan, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(45): [FBrf0200374]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase analysis	FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433] Curators, 2008--, Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. Assigning Gene Ontology (GO) terms by sequence similarity in FlyBase. [FBrf0202953]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]