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Gene Dmel\TwdlD

General Information
Symbol	Dmel\TwdlD	Species	D. melanogaster
Name	TweedleD	Annotation symbol	CG14243
Feature type	protein_coding_gene	FlyBase ID	FBgn0039444
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	97C3-97C3	Sequence location	3R:22,465,747..22,466,776 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene TweedleD is referred to in FlyBase by the symbol Dmel\TwdlD (CG14243, FBgn0039444). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: structural constituent of chitin-based larval cuticle. There is experimental evidence that it is involved in the biological process: body morphogenesis; chitin-based cuticle development. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Domain of unknown function DUF243. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1636097_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of TwdlD. Gene sequence location is 3R:22465747..22466776.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 97C3-97C3   Limits computationally determined from genome sequence between P{lacW}scribj7B3 and P{lacW}His2AvL1602
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\TwdlD for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) TwdlD-RA FBtr0085035  NM_143235   965   256
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein TwdlD-PA   FBpp0084407   27.7   256   5.85     NP_651492   AAF56610
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56610   AY071417 AAL49039   BI162840   BI163719   BI166355   BI168620   BI169033   BI169295   BI169370   BI169580   BI173790   BI173859   BI214659   BI227479   BI229749   BI231623   BI232574   BI233258   BI235738   BI238611   BI241594   BI241993   BI363651   BI364675   BI364727   BI364995   BI367758   BI369025   BI369523   BI370337   BI371486   BI374916   BI375396   BI375929   BI481966   BI481977   BI577631   BI580136   BP552572   BP552573   CO176823   CO270709   CO281564   CO285524   CO297877   CO310907   CO318128   CO323746   EC080975   EC085546   EC089652   EC199465   EC199908   EC200743   EC220805   EC225176   EC260216   ES696180   FD586251   FD586595   GH740122   GH740917   GH741348   GH742872   GH743169   GH743584   GH743665   GH744878   GH745084   GH745167   GH746715   GH763494   GH764367   GH764540   GH765089   GH765629   GH766189   GH767617   GH768668   GH768885   GH768918
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VBD2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 16   dorsal head epidermis   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
proteinaceous extracellular matrix inferred from direct assay (Guan et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1.3 Larval Midgut    5.7 Larval Hindgut    1.6 Larval Malpighian Tubules    5.6 Larval Fat Body    6.9 Larval Salivary Gland    7.8 Larval Trachea    5.125 Larval Carcass    7.925 Adult Head    3.2 Adult Eye    5.95 Adult Brain    1.3 Adult Thoracic-Abdominal Ganglion    2.4 Adult Crop    10 Adult Midgut    6.7 Adult Hindgut    2.4 Adult Malpighian Tubules    2.1 Adult Fat Body    1.8 Adult Salivary Gland    8.1 Adult Heart    4.325 Adult VirginFemale Spermatheca    6.1 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    1.5 Adult Testis    2.5 Adult Male Accessory Gland    9.2 Adult Carcass    7.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    3.2   NA  Eye    5.95   NA  Brain    1.3   1.3  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.4   NA  Crop    no informative data   5.7  Midgut    6.7   1.6  Hindgut    2.4   5.6  Malpighian Tubules    2.1   6.9  Fat Body    1.8   7.8  Salivary Gland    8.1   NA  Heart    4.325   5.125  Trachea    NA   NA  VirginFemale Spermatheca    6.1   NA  InseminatedFemale Spermatheca    5.1   NA  Ovary    1.5   NA  Testis    2.5   NA  Male Accessory Gland    9.2   7.925  Carcass    7.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr    266 embryo 16-18hr    2710 embryo 18-20hr    2127 embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2    243 larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr  (266) embryo 16-18hr  (2710) embryo 18-20hr  (2127) embryo 20-22hr  (37) embryo 22-24hr  (53) larva L1    5 larva L2  (243) larva L3 12hr old  (52) larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr  (266) embryo 16-18hr  (2710) embryo 18-20hr  (2127) embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2  (243) larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr    266 embryo 16-18hr    2710 embryo 18-20hr    2127 embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2    243 larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr    266 embryo 16-18hr    2710 embryo 18-20hr    2127 embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2    243 larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr  (266) embryo 16-18hr  (2710) embryo 18-20hr  (2127) embryo 20-22hr  (37) embryo 22-24hr  (53) larva L1    5 larva L2  (243) larva L3 12hr old  (52) larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr  (266) embryo 16-18hr  (2710) embryo 18-20hr  (2127) embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2  (243) larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    3 embryo 04-06hr    2 embryo 06-08hr    2 embryo 08-10hr    10 embryo 10-12hr    14 embryo 12-14hr    11 embryo 14-16hr    266 embryo 16-18hr    2710 embryo 18-20hr    2127 embryo 20-22hr    37 embryo 22-24hr    53 larva L1    5 larva L2    243 larva L3 12hr old    52 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    2 larva L3 puffstage 7-9    3 white prepupae new    2 white prepupae 12hr    4 white prepupae 24hr    6 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    13 adult male 01day    13 adult male 05day    17 adult male 30day    20 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    0 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    11 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    0 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    1 Ethanol 5% 3 hrs, larvae L3    0 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    0 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    7 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    17 Paraquat 10 mM 48 hrs, 4-day adult    17 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_12 mE1_20_mRNA_expression_cluster_12
External Data & Images
Linkouts	BDGP expression data • RE49895 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039444 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14243-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for TwdlD Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 TwdlDGD6292 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of TwdlD Class Mutagen Stocks Known lesion TwdlD1 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of TwdlD Class Mutagen Stocks Known lesion TwdlDGD6292 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TwdlDKK113105 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes TwdlDAct5C.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TwdlDNIG.14243R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes TwdlDT:Disc\RFP-DsRed2 in vitro construct - coding region fusion 0 Yes TwdlDT:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes TwdlDΔ173-175.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK113105} NA P{NIG.14243R} NA characterization construct P{TwdlD1-RFP,Tb1-RFP} NA P{TwdlD-RFP} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function
CV term Evidence References
structural constituent of chitin-based larval cuticle inferred from direct assay (Guan et al., 2006)
Biological Process
CV term Evidence References
body morphogenesis inferred from mutant phenotype (Guan et al., 2006) chitin-based cuticle development inferred from mutant phenotype (Guan et al., 2006)
Cellular Component
CV term Evidence References
proteinaceous extracellular matrix inferred from direct assay (Guan et al., 2006)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions TwdlD allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68105 DroID - A comprehensive database of gene and protein interactions. • FBgn0039444 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43210
Orthologs
OrthoDB Orthologs (12) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6X0NCP Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6X0NCP)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\TwdlD     Drosophila simulans   Dsim\GD21297 Y   Drosophila sechellia   Dsec\GM10337 Y   Drosophila erecta   Dere\GG11494 Y   Drosophila yakuba   Dyak\GE23685 Y   Drosophila ananassae   Dana\GF16449 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA12855 Y   Drosophila persimilis   Dper\GL23105 Y   Drosophila willistoni   Dwil\GK13324 Y   Drosophila virilis   Dvir\GJ14179 Y   Drosophila mojavensis   Dmoj\GI22884 Y   Drosophila grimshawi   Dgri\GH18732 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21297 Drosophila sechellia Dsec\GM10337 Drosophila erecta Dere\GG11494 Drosophila yakuba Dyak\GE23685 Drosophila ananassae Dana\GF16449 Drosophila pseudoobscura pseudoobscura Dpse\GA12855 Drosophila persimilis Dper\GL23105 Drosophila willistoni Dwil\GK13324 Drosophila virilis Dvir\GJ14179 Drosophila mojavensis Dmoj\GI22884 Drosophila grimshawi Dgri\GH18732
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v105039 P{KK113105}VIE-260B v14793 w1118; P{GD6292}v14793
Genomic Clones ( 2 )
	BACR08N03 BACR13P17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 77 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE49895
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE02083 RE03195 RE06458 RE09438 RE09991 RE10430 RE10525 RE10795 RE16894 RE16980 RE20643 RE22918 RE24537 RE27591 RE28660 RE29557 RE31659 RE35114 RE38708 RE39206 RE48558 RE49842 RE50210 RE53593 RE55106 RE55731 RE56816 RE58390 RE62609 RE63179 RE63835 RE64589 RE64612 RE71342 RE74276
Other clones	10595 42718 53472 56461 64868 100265 165868 214503.9 CK01.63C.C12 EK051244 EK100008 EK157447 EK169157 EK170820 EK191335 EK222943 EK270419 FGM219E08 RM01018 RM01418 RP001002599 RP001003499 RP001010651 RP001019253 RP001020234 RP001022779 RP001023928 RP001024409 RP001029277 RP001036020 RP001041273 RP001043681 RP001045999 RP001046363 RP001047647 RP001048226 RP001049517 RP001049922 RP001051279 RP001051423 RP001056536
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039444 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43210
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: TwdlD CG14243 (Guan, 2006, Guan et al., 2006)
Source for database merge of
Additional comments
Other Comments
	TwdlD affects body shape throughout the end of embryogenesis and through the pupal stage. (Guan et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143235 RefSeq (Proteins) • NP_651492 DDBJ / EMBL / GenBank • AAF56610 AAL49039 AY071417 BI364727 Entrez Gene - A searchable database of RefSeq genes. • 43210 UniProtKB/TrEMBL • Q9VBD2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF243 (IPR004145) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE49895
Linkouts
	BioGRID - A database of protein and genetic interactions • 68105 DroID - A comprehensive database of gene and protein interactions. • FBgn0039444 DRSC - Results from RNAi screens. • FBgn0039444 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039444 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14243-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039444 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43210 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43210 modMine - Data generated by the modENCODE project. • FBgn0039444
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG14243 (Guan, 2006, Guan et al., 2006, Keleman et al., 2009.8.5, Tomancak et al., 2007, Japanese National Institute of Genetics, 2012.5.21) TwdlD (Guan, 2006, Guan et al., 2006, Kallappagoudar et al., 2010, Pina and Pignoni, 2012)
Name Synonym	CG14243   TweedleD (Guan, 2006, Guan et al., 2006, Cornman, 2010)
Secondary FlyBase IDs
References ( 16 )
Research paper	Pina and Pignoni, 2012, genesis 50(2): 119--123 Tubby-RFP Balancers for Developmental Analysis: FM7c 2xTb-RFP, CyO 2xTb-RFP, and TM3 2xTb-RFP. [FBrf0217414] Cornman, 2010, PLoS ONE 5(9): e12536 The Distribution of GYR- and YLP-Like Motifs in Drosophila Suggests a General Role in Cuticle Assembly and Other Protein-Protein Interactions. [FBrf0211774] Kallappagoudar et al., 2010, Mol. Cell. Proteomics 9(9): 2005--2018 Nuclear Matrix Proteome Analysis of Drosophila melanogaster. [FBrf0211714] Tomancak et al., 2007, Genome Biol. 8(7): R145 Global analysis of patterns of gene expression during Drosophila embryogenesis. [FBrf0205271] Guan et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(45): 16794--16799 Mutation of TweedleD, a member of an unconventional cuticle protein family, alters body shape in Drosophila. [FBrf0195309]
Supplementary material	Graveley et al., 2011, Nature 471(7339): Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980] The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Guan, 2006, Proc. Natl. Acad. Sci. U.S.A. 103(45): [FBrf0200374] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file) Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
FlyBase analysis	FlyBase Curators, 2010-, Gene Ontology (GO) annotation review. Gene Ontology (GO) annotation review. [FBrf0216433]
Stock list	Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY. RNAi stocks available from NIG-FLY. [FBrf0218457]
Website	Fisher et al., 2012, BDGP insitu homepage BDGP insitu homepage [FBrf0219073]