FB2022_02, released March 29, 2022

Gene: Dmel\mfrn

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General Information
Symbol
Dmel\mfrn
Species
D. melanogaster
Name
mitoferrin
Annotation Symbol
CG4963
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039561
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly. (UniProt, Q9VAY3)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

dmfrn

Key Links
Genomic Location
Cytogenetic map
98B6-98B6
Sequence location
Recombination map
3-94
RefSeq locus
NT_033777 REGION:27942796..27948021
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables iron ion transmembrane transporter activity
inferred from direct assay
(assigned by UniProt )
(Metzendorf et al., 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables iron ion transmembrane transporter activity
inferred from biological aspect of ancestor with PANTHER:PTN000641807
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in iron import into the mitochondrion
inferred from direct assay
(assigned by UniProt )
(Metzendorf et al., 2009)
involved_in multicellular organismal iron ion homeostasis
inferred from mutant phenotype
(Navarro et al., 2015)
involved_in spermatid differentiation
inferred from mutant phenotype
(Metzendorf and Lind, 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in iron import into the mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000641807
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in mitochondrial inner membrane
inferred from direct assay
(assigned by UniProt )
(Metzendorf et al., 2009)
located_in mitochondrion
inferred from direct assay
(Metzendorf et al., 2009)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the mitochondrial carrier (TC 2.A.29) family. (Q9VAY3)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
SLC25 FAMILY OF MITOCHONDRIAL TRANSPORTERS -
The solute carrier family 25 (SLC25) or mitochondrial carrier family (MCF) members shuttle metabolites, nucleotides and cofactors across the inner mitochondrial membrane. (Adapted from FBrf0212481, FBrf0212949 and PMID:23266187).
Protein Function (UniProtKB)
Mitochondrial iron transporter that mediates iron uptake. Probably required for heme synthesis of hemoproteins and Fe-S cluster assembly.
(UniProt, Q9VAY3)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\mfrn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VAY3)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mfrn-RA
FBtr0085266
NM_143343
3037
379
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
mfrn-PA
FBpp0084635
41.8
379
8.96
NP_651600
AAF56764
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mfrn using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male & day 3 of adulthood
adult head
(Metzendorf and Lind, 2010)
adult thorax
(Metzendorf and Lind, 2010)
testis
(Metzendorf and Lind, 2010)
adult Malpighian tubule
(Metzendorf and Lind, 2010)
adult gut
(Metzendorf and Lind, 2010)
Additional Descriptive Data

mfrn is expressed at similar levels to whole fly in all tissues tested except testis where it is 2-fold higher. This is in contast to Fly Atlas which shows the lowest levels in testis.

(Metzendorf and Lind, 2010)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in mitochondrial inner membrane
inferred from direct assay
(assigned by UniProt )
(Metzendorf et al., 2009)
located_in mitochondrion
inferred from direct assay
(Metzendorf et al., 2009)
Expression Deduced from Reporters
Reporter: P{lacW}mfrnSH115
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
spermatid
(Metzendorf and Lind, 2010)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mfrn in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of mfrn
Transgenic constructs containing regulatory region of mfrn
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Df(3R)ED6277
(Metzendorf and Lind, 2010)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\SLC25A37
14 of 15
Yes
Yes
1  
Hsap\SLC25A28
12 of 15
No
Yes
Hsap\NARS2
1 of 15
No
No
1  
Hsap\NARS
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Slc25a28
12 of 15
Yes
Yes
Mmus\Gm16867
8 of 15
No
Yes
Mmus\Slc25a37
8 of 15
No
Yes
Mmus\Nars
1 of 15
No
No
Rattus norvegicus (Norway rat) (4)
Rnor\Slc25a37
12 of 13
Yes
Yes
Rnor\Slc25a28
11 of 13
No
Yes
Rnor\Nars2
1 of 13
No
No
Rnor\Nars
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
Xtro\slc25a37
12 of 12
Yes
Yes
Xtro\slc25a28
11 of 12
No
Yes
Xtro\nars2
1 of 12
No
No
Xtro\nars
1 of 12
No
No
Danio rerio (Zebrafish) (4)
Drer\slc25a37
13 of 15
Yes
Yes
Drer\slc25a28
12 of 15
No
Yes
Drer\nars2
1 of 15
No
No
Drer\nars
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\mfn-1
14 of 15
Yes
Yes
Cele\nars-1
1 of 15
No
No
Cele\nars-2
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\AT1G07030
9 of 9
Yes
Yes
Atha\AT2G30160
9 of 9
Yes
Yes
Atha\AT1G07025
1 of 9
No
Yes
Atha\AT1G68420
1 of 9
No
Yes
Atha\NS1
1 of 9
No
No
Atha\NS2
1 of 9
No
No
Atha\SYNC1
1 of 9
No
No
Atha\SYNC3
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\MRS3
14 of 15
Yes
Yes
Scer\MRS4
13 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC8C9.12c
11 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (45)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Ant2
1 of 10
aralar1
1 of 10
AsnRS
1 of 10
AsnRS-m
1 of 10
Bmcp
1 of 10
CG1628
1 of 10
CG1907
1 of 10
CG2616
1 of 10
CG4743
1 of 10
CG4995
1 of 10
CG5254
1 of 10
CG5805
1 of 10
CG6893
1 of 10
CG7514
1 of 10
CG7943
1 of 10
CG8026
1 of 10
CG8323
1 of 10
CG9582
1 of 10
CG16736
1 of 10
CG18324
1 of 10
CG18327
1 of 10
CG18418
1 of 10
colt
1 of 10
Dic1
1 of 10
Dic2
1 of 10
Dic3
1 of 10
Dic4
1 of 10
DPCoAC
1 of 10
GC1
1 of 10
GC2
1 of 10
MME1
1 of 10
Mpcp1
1 of 10
Mpcp2
1 of 10
PMP34
1 of 10
Rim2
1 of 10
SCaMC
1 of 10
sea
1 of 10
sesB
1 of 10
Shawn
1 of 10
Tpc1
1 of 10
Tpc2
1 of 10
Tyler
1 of 10
Ucp4A
1 of 10
Ucp4B
1 of 10
Ucp4C
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 4 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    SLC25A37; solute carrier family 25 member 37
    14 of 15
    610387
         
        SLC25A28; solute carrier family 25 member 28
        12 of 15
        609767
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            mfrn - Drep2
            anti tag coimmunoprecipitation, peptide massfingerprinting
            (Andlauer et al., 2014)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            fh
            suppressible
            mfrn
            (Edenharter et al., 2017, Navarro et al., 2015)
            fh
            enhanceable
            mfrn
            (Navarro et al., 2015)
            External Data
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            External Data
            Linkouts
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-94
            Cytogenetic map
            98B6-98B6
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            98B6-98B6
            Limits computationally determined from genome sequence between P{PZ}btzrL203 and P{PZ}l(3)0648706487
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-94

            (FlyBase, 2016)

            3-92.4

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (8)
            Genomic Clones (7)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (204)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: mfrn CG4963

            (Metzendorf et al., 2009)
            Source for database merge of

            Source for merge of: CG4963 anon- EST:fe2H5

            (FlyBase, 1996-)
            Additional comments

            Source for merge of CG4963 anon- EST:fe2H5 was sequence comparison ( date:000605 ).

            (FlyBase, 1996-)
            Other Comments

            Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

            (Claridge-Chang et al., 2001)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 42 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Synonyms and Secondary IDs (9)
            Datasets (8)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (8)
            Result
            Result Type
            Title
            scRNAseq_2020_Cattenoz_NI_seq_clustering
            Clustering analysis of hemocytes from non-infested third instar larvae
            scRNAseq_2020_Cattenoz_WI_seq_clustering
            Clustering analysis of hemocytes from wasp-infested third instar larvae
            scRNAseq_2020_Cho_NI72LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
            scRNAseq_2020_Cho_NI96LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
            scRNAseq_2020_Cho_WI96LG_seq_clustering
            Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI96HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
            References (60)