FlyBase Gene Report: Dmel\Cad99C

General Information

Symbol

Dmel\Cad99C

Species

D. melanogaster

Name

Cadherin 99C

Annotation Symbol

CG31009

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039709

Gene Model Status

Stock Availability

20 publicly available

Gene Snapshot

Cadherin 99C (Cad99C) encodes a member of the cadherin superfamily of transmembrane proteins, which mediate cell-cell adhesion and/or cell-cell communication. Its roles include microvillus morphogenesis, tube elongation, endosome formation and immunity. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

CA-10

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:29,841,657..29,854,699 [+]

Recombination map

3-99

RefSeq locus

NT_033777 REGION:29841657..29854699

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables myosin binding

inferred from physical interaction with FLYBASE:ck; FB:FBgn0000317

(Glowinski et al., 2014)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR002126, InterPro:IPR015919

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Hill et al., 2001)

Biological Process (13 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in apical protein localization

inferred from mutant phenotype

(Chung and Andrew, 2014)

involved_in calcium-mediated signaling

inferred from genetic interaction with FLYBASE:norpA; FB:FBgn0262738

(Lee et al., 2015)

involved_in chorion-containing eggshell formation

inferred from mutant phenotype

(D'Alterio et al., 2005)

involved_in follicle cell microvillus organization

inferred from mutant phenotype

(Schlichting et al., 2006, D'Alterio et al., 2005)

involved_in mucosal immune response

inferred from mutant phenotype

(Lee et al., 2015)

inferred from genetic interaction with FLYBASE:norpA; FB:FBgn0262738

(Lee et al., 2015)

involved_in positive regulation of clathrin-dependent endocytosis

inferred from mutant phenotype

(Lee et al., 2015)

involved_in regulation of follicle cell microvillus length

inferred from mutant phenotype

(D'Alterio et al., 2005)

involved_in tube morphogenesis

inferred from mutant phenotype

(Chung and Andrew, 2014)

involved_in vitelline membrane formation involved in chorion-containing eggshell formation

inferred from mutant phenotype

(Schlichting et al., 2006)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules

non-traceable author statement

(assigned by UniProt )

(Hill et al., 2001)

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

involved_in cell-cell adhesion mediated by cadherin

inferred from sequence model

(Hill et al., 2001)

involved_in cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN001931898

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in homophilic cell adhesion via plasma membrane adhesion molecules

inferred from electronic annotation with InterPro:IPR002126

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Schlichting et al., 2006)

colocalizes_with brush border membrane

inferred from direct assay

(Lee et al., 2015)

colocalizes_with endosome

inferred from direct assay

(Lee et al., 2015)

located_in microvillus

inferred from direct assay

(Schlichting et al., 2006, D'Alterio et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in integral component of plasma membrane

inferred from sequence or structural similarity

(Hynes and Zhao, 2000)

non-traceable author statement

(assigned by UniProt )

(Hill et al., 2001)

Gene Group (FlyBase)

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CADHERINS -: The cadherin superfamily represent a diverse group of transmembrane receptors which mediate cell-cell adhesion. Cadherins are defined by the presence of cadherin domains, typically organised in tandem repeats, which mediate calcium-dependent homophilic interactions between cadherin molecules. (Adapted from FBrf0146675).

Protein Function (UniProtKB)

Cadherin that functions in epithelial morphogenesis and the intestine epithelial immune response (PubMed:16260500, PubMed:16507588, PubMed:24718992, PubMed:25639794). Essential for female fertility (PubMed:16260500, PubMed:16507588). Regulates the length and organization of apical microvilli in developing follicle cells and salivary glands (PubMed:16260500, PubMed:16507588, PubMed:24718992, PubMed:25236597). Function in the follicle cell is essential for egg development as the microvilli secrete eggshell material such as the vitelline membrane (PubMed:16260500, PubMed:16507588, PubMed:25236597). Acts at least in part by regulating the recruitment of the myosin ck to the follicle cell microvilli (PubMed:25236597). Also required to regulate cell rearrangements during salivary tube elongation, possibly by modulating cellular adhesion between the apical surface and apical extracellular matrix during epithelial tube elongation (PubMed:24718992). May also function in cellular adhesion during the development of other tubular epithelia such as the trachea (PubMed:24718992). Possibly functions as an apical membrane determinant which acts in apical membrane expansion during salivary and tracheal epithelial tube elongation (PubMed:24718992). In salivary gland development, this function is independent of the other apical membrane determinants crb and sas (PubMed:24718992). Essential downstream component of a hh-signaling pathway which regulates the Duox-dependent gut epithelial immune response to bacterial uracil; required for endosome formation in the enterocyte and activating norpA-dependent Ca2+ mobilization, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection (PubMed:25639794).

(UniProt, Q9VAF5)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Cad99C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0085488

5795

1706

FBtr0303095

5651

1706

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0084854

186.9

1706

4.50

FBpp0292214

186.9

1706

4.50

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1706 aa isoforms: Cad99C-PA, Cad99C-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts (via the cytoplasmic domain) with ck (PubMed:25236597, PubMed:27331610). Interacts (via the cytoplasmic domain) with Cul1 and Ubr3 (PubMed:27331610).

(UniProt, Q9VAF5)

Domain

The extracellular domain is necessary for conferring apical characteristics on the salivary gland membrane (PubMed:24718992). Necessary for regulating salivary gland membrane expansion and tube length, but is not required for cell rearrangement during tube elongation (PubMed:24718992). Sufficient to promote the outgrowth of follicle cell microvilli and the formation of microvilli bundles (PubMed:16260500, PubMed:24718992) Sufficient to promote formation of microvilli-like structures in the salivary glands and the follicle cell microvilli bundles (PubMed:16507588, PubMed:24718992). Dispensable for apical localization (PubMed:24718992).

The cytoplasmic domain is necessary for the formation of uracil-induced endosomes.

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain (By similarity).

(UniProt, Q9VAF5)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cad99C using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cad99C_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S4 -- S8

main body follicle cell

(Zartman et al., 2009)

polar follicle cell

(Schlichting et al., 2006)

oogenesis stage S4 -- S13

polar follicle cell

(Zartman et al., 2009)

oogenesis stage S10B

border follicle cell

(Zartman et al., 2009)

oogenesis stage S2 -- S14

(Schlichting et al., 2006)

oogenesis stage S9 -- S14

main body follicle cell

(Schlichting et al., 2006)

Additional Descriptive Data

Cad99C is expressed in oogenesis stages S4-8 in the anterior and posterior follicle cells and in follicle cells that migrate to contact the ooctye at stage S9.

(Zartman et al., 2009)

Cad99C is detected in follicle cells at the anterior and posterior poles of the egg chamber at oogenesis stages 4-8. During stages 9-14, signal is detected in follicle cells surrounding the oocyte.

(Schlichting et al., 2006)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Demontis and Dahmann, 2009)

oogenesis stage S8 & adult stage | female

oocyte | posterior

(Talamillo et al., 2013)

oogenesis & adult stage | female

main body follicle cell | apical

(Khanal et al., 2016)

oogenesis stage S2 -- S14

(Schlichting et al., 2006)

oogenesis stage S9 -- S14

main body follicle cell

(Schlichting et al., 2006)

oogenesis stage S4 -- S8

polar follicle cell

(Schlichting et al., 2006)

Additional Descriptive Data

Cad99C protein is detected at the border between follicle cells and the oocyte at the anterior and posterior poles of the egg chamber at oogenesis stages 4-8. During stages 9-14, signal is detected in structures at the entire border between the oocyte and the follicle cells.

(Schlichting et al., 2006)

Marker for

(Glowinski et al., 2014)

Subcellular Localization

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Schlichting et al., 2006)

colocalizes_with brush border membrane

inferred from direct assay

(Lee et al., 2015)

colocalizes_with endosome

inferred from direct assay

(Lee et al., 2015)

located_in microvillus

inferred from direct assay

(Schlichting et al., 2006, D'Alterio et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cad99C in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cad99C

View exonic expression by tissue for Dmel\Cad99C

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039709

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0039709

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG31009-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31009

Flygut - An atlas of the Drosophila adult midgut

FBgn0039709

Images

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 25 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cad99C

Transgenic constructs containing regulatory region of Cad99C

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Cad99C^GD153

Cad99C^57A

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Sterility

Allele

female semi-fertile

Cad99C^GE23478

female semi-sterile

Cad99C^57A

Cad99C^248A

Other Phenotypes

Allele

cell adhesion defective, with Scer\GAL4^fkh.PH

flight defective, with Scer\GAL4^Mef2.PR

Cad99C^GD153

majority die during embryonic stage (with Cad99C^57A)

Cad99C^120B

majority die during embryonic stage (with Cad99C^120B)

Cad99C^57A

some die during pupal stage, with Scer\GAL4^pnr-MD237

Cad99C^GD153

visible, with Scer\GAL4^pnr-MD237

Cad99C^GD153

Phenotype manifest in

Allele

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

embryonic/larval salivary gland (with Cad99C^57A)

Cad99C^120B

embryonic/larval salivary gland (with Cad99C^120B)

Cad99C^57A

embryonic/larval trachea, with Scer\GAL4^btl.PS

endosome, with Scer\GAL4^NP0001

Cad99C^{Δcyto.UAS.GFP}

follicle cell | somatic clone

Cad99C^57A

follicle cell | somatic clone, with Scer\GAL4^Act5C.PP

Johnston organ | P-stage | somatic clone

Cad99C^57A

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Cad99C^GD153

microvillus & follicle cell, with Cad99C^21-5/Cad99C^21-6, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^{Δcyt.UAS.EGFP}

microvillus & follicle cell, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

microvillus & follicle cell (with Cad99C^21-5), with Cad99C^{Δcyt.UAS.EGFP}, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^21-6

microvillus & follicle cell (with Cad99C^21-6)

Cad99C^21-8

microvillus & follicle cell (with Cad99C^21-6), with Cad99C^{Δcyt.UAS.EGFP}, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^21-5

microvillus & follicle cell (with Cad99C^21-8)

Cad99C^21-6

microvillus | somatic clone

Cad99C^57A

microvillus | somatic clone, with Scer\GAL4^Act5C.PP

midgut, with Scer\GAL4^NP0001

Cad99C^{Δcyto.UAS.GFP}

scolopidium | P-stage | somatic clone

Cad99C^57A

seminal vesicle

Cad99C^248A

Cad99C^248A

Cad99C^248A

Cad99C^248A

tracheal precursor cell, with Scer\GAL4^btl.PS

trichogen cell, with Scer\GAL4^pnr-MD237

Cad99C^GD153

vitelline membrane

vitelline membrane, with Cad99C^21-5/Cad99C^21-6, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^{Δcyt.UAS.EGFP}

vitelline membrane, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

vitelline membrane (with Cad99C^21-5), with Cad99C^{Δcyt.UAS.EGFP}, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^21-6

vitelline membrane (with Cad99C^21-6), with Cad99C^{Δcyt.UAS.EGFP}, Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Cad99C^21-5

vitelline membrane (with Cad99C^51C)

vitelline membrane (with Cad99C^57A)

vitelline membrane (with Cad99C^120B)

vitelline membrane | somatic clone, with Scer\GAL4^Act5C.PP

Cad99C^UAS.EGFP

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

9 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (5)

4 of 13

Yes

Yes

Rnor\NEWGENE_1590969

2 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (4)

3 of 12

Yes

Yes

1 of 12

No

No

1 of 12

No

No

Xtro\LOC100495486

1 of 12

No

Yes

Danio rerio (Zebrafish) (6)

8 of 15

Yes

Yes

6 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (3)

8 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900HP )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00008496

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500B9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY009483

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14006

Mayetiola destructor

Hessian fly

Mdes\Mdes005417

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008421

Anopheles darlingi

American malaria mosquito

Adar\ADAC005733

Anopheles gambiae

Malaria mosquito

Agam\AGAP002310

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ005081

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0E6O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA006250

Danaus plexippus

Monarch butterfly

Dple\DPOGS210669-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL003928

Heliconius melpomene

Postman butterfly

Hmel\HMEL018086

Apis florea

Little honeybee

Aflo\2147574099

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002af5

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008551

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008563

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM580250

Rhodnius prolixus

Kissing bug

Rpro\RPRC006699

Cimex lectularius

Bed bug

Clec\CLEC009465

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI086258

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08070

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0E5C )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80883.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80881.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g04060

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_301624

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0MMW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-CadherinL2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Protocad15L

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000002744

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

PCDH15; protocadherin related 15

model of autosomal recessive nonsyndromic deafness 23

IEA

(FlyBase, 2019-)

model of Usher syndrome type 1D

IEA

(FlyBase, 2019-)

model of Usher syndrome type 1F

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PCDH15; protocadherin related 15

11 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2016)

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Li et al., 2016, Glowinski et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lee et al., 2018)

suppressible

(Eren-Ghiani et al., 2015)

suppressible

(Chung and Andrew, 2014)

suppressible

(Lee et al., 2018)

suppressible

(Lee et al., 2018)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Ismat et al., 2013)

suppressible

(Lee et al., 2015)

suppressible

(Lee et al., 2015)

suppressible

(Lee et al., 2015)

enhanceable

(Li et al., 2016)

External Data

Subunit Structure (UniProtKB)

Interacts (via the cytoplasmic domain) with ck (PubMed:25236597, PubMed:27331610). Interacts (via the cytoplasmic domain) with Cul1 and Ubr3 (PubMed:27331610).

(UniProt, Q9VAF5 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0039709

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43528

MIST (protein-protein) - An integrated Molecular Interaction Database

Cad99C

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-99

Cytogenetic map

Sequence location

3R:29,841,657..29,854,699 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99C3-99C4

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-99

(FlyBase, 2016)

3-97.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

67199

67200

Cad99C^248A/TM6B, Tb¹

26413

w¹¹¹⁸; Mi{ET1}Cad99C^MB08891

27510

y¹ v¹; P{TRiP.JF02660}attP2

67198

P{neoFRT}82B Cad99C^57A/TM6B, Tb¹

v3733

w¹¹¹⁸; P{GD151}v3733

v3739

w¹¹¹⁸; P{GD153}v3739

v27211

w¹¹¹⁸; P{GD14471}v27211

v27212

w¹¹¹⁸; P{GD14471}v27212

v27215

w¹¹¹⁸; P{GD14472}v27215

v27216

w¹¹¹⁸; P{GD14472}v27216

v318428

PBac{fTRG10245.sfGFP-nlsTTSTF}VK00033

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (19)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039709

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039709

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43528

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2015, Glowinski et al., 2014)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Cad99C CA-10

(Demontis, 2004.11.6)

Source for database merge of

Source for merge of: CG7805 CG15511

(Hill et al., 2001)

Additional comments

Annotations CG15511, CG7805 merged as CG31009 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

The extracellular (and transmembrane) domain of Cad99C is sufficient to confer apical character on membranes but does not affect cell rearrangement during tube elongation.

(Chung and Andrew, 2014)

Loss of Cad99C results in shorter microvilli and overexpression in longer microvilli than in wild-type, indicating that the concentration of Cad99C is positively correlated with the length of microvilli in follicle cells.

(D'Alterio et al., 2005)

Cad99C is regulated by hh.

(Schlichting et al., 2005)

Expression is highly increased in the A/P organizer region.

(Demontis et al., 2003)

Cad99C is a hh target gene that might play an important role during wing tissue patterning and larval development.

(Demontis et al., 2003)

Overexpression of Cad99C is associated with the genesis/stabilization of long apical membrane protrusions. RNAi-mediated loss of Cad99C function is associated with a developmental block at the larval stage.

(Demontis et al., 2003)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43528

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VAF5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q4L2X0

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039709

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039709

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cad99C_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31009

Flygut - An atlas of the Drosophila adult midgut

FBgn0039709

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43528

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011374

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG31009

modMine - A data warehouse for the modENCODE project

FBgn0039709

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0039709

DRSC - Results frm RNAi screens

FBgn0039709

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0039709

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG31009-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039709

FlyMine - An integrated database for Drosophila genomics

FBgn0039709

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43528

MIST (protein-protein) - An integrated Molecular Interaction Database

Cad99C

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CA-10

(Demontis, 2004.11.6, Demontis et al., 2003)

CG15511

(Hill et al., 2001)

CG310034

(D'Alterio et al., 2005)

CG31009

(Ni et al., 2011.7.19, Perkins et al., 2009)

CG7805

(Whitfield, 2000.5.15)

CT35624

(Hynes and Zhao, 2000)

CT4930

(Hynes and Zhao, 2000)

Cad99C

(Chmiel et al., 2019, Hall et al., 2019, Lattner et al., 2019, Li et al., 2018, Transgenic RNAi Project members, 2017-, Gene Disruption Project members, 2016-, Kuleesha et al., 2016, Sarov et al., 2016, Eren-Ghiani et al., 2015, Gene Disruption Project members, 2015-, Lee et al., 2015, Lee et al., 2015, Wangler et al., 2015, Ashwal-Fluss et al., 2014, Chung and Andrew, 2014, Glowinski et al., 2014, Ismat et al., 2013, Talamillo et al., 2013, Sarpal et al., 2012, Zhai et al., 2012, Monier et al., 2011, Yan et al., 2011, Zappia et al., 2011, Blumenstiel et al., 2009, Demontis and Dahmann, 2009, Genevet et al., 2009, Zartman et al., 2009, Elalayli et al., 2008, Fung et al., 2008, Zartman et al., 2008, Demontis et al., 2006, Schlichting et al., 2006, D'Alterio et al., 2005, Schlichting et al., 2005, Demontis et al., 2003)

Cad99c

(Napoletano et al., 2011)

DCad99C

(Tepass et al., 2000)

Dm Cad99C

(Demontis et al., 2006)

cad99C

(Godt et al., 2005, Schlichting et al., 2005)

Name Synonyms

Cad99C

(Fung et al., 2008)

Cadherin 99C

(Lee et al., 2015, Chung and Andrew, 2014, Monier et al., 2011)

Cadherin99C

(Ismat et al., 2013)

Secondary FlyBase IDs

FBgn0039708
FBgn0051009

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (78)