FB2020_03, released Jun 16, 2020

Gene: Dmel\dgt1

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General Information
Symbol
Dmel\dgt1
Species
D. melanogaster
Name
dim γ-tubulin 1
Annotation Symbol
CG18041
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039710
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

NSL2, non-specific lethal 2

Key Links
Genomic Location
Cytogenetic map
99C4-99C4
Sequence location
3R:29,854,695..29,857,491 [-]
Recombination map

3-99

RefSeq locus
NT_033777 REGION:29854695..29857491
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
contributes_to histone acetyltransferase activity (H4-K16 specific)
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
contributes_to histone acetyltransferase activity (H4-K5 specific)
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
contributes_to histone acetyltransferase activity (H4-K8 specific)
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
histone H4-K16 acetylation
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
histone H4-K5 acetylation
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
histone H4-K8 acetylation
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
NSL complex
inferred from direct assay
(Raja et al., 2010)
nucleus
inferred from direct assay
(Raja et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
NSL complex
inferred from biological aspect of ancestor with PANTHER:PTN001003176
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
HISTONE ACETYLTRANSFERASES -
Histone acetyltransferases catalyze the acetylation of conserved lysine residues on histone proteins by transferring an acetyl group from acetyl CoA to form ε-N-acetyl lysine. (Adapted from PMID:11395403).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\dgt1 or the JBrowse view of Dmel\dgt1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.47

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
dgt1-RA
FBtr0085527
NM_143480
1712
484
dgt1-RB
FBtr0335006
NM_001276154
1687
425
dgt1-RC
FBtr0339942
NM_001300666
2231
589
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
dgt1-PA
FBpp0084893
56.0
484
8.15
NP_651737
AAF56956
dgt1-PB
FBpp0307014
49.4
425
8.83
NP_001263083
AGB96463
dgt1-PC
FBpp0308967
67.4
589
4.93
NP_001287595
AHN57594
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dgt1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
NSL complex
inferred from direct assay
(Raja et al., 2010)
nucleus
inferred from direct assay
(Raja et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dgt1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dgt1
Transgenic constructs containing regulatory region of dgt1
Deletions and Duplications ( 1 )
Not disrupted in
Df(3R)Drice
(Xu et al., 2006)
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\KANSL2
13 of 15
Yes
Yes
Hsap\INO80D
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Kansl2
15 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\LOC103690317
6 of 13
Yes
Yes
Rnor\Kansl2
5 of 13
No
Yes
Rnor\Ino80d
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\kansl2
9 of 12
Yes
Yes
Xtro\ino80d
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\kansl2
14 of 15
Yes
Yes
Drer\ino80db
2 of 15
No
No
Drer\LOC110440037
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (2)
Atha\AT2G31600
1 of 9
Yes
Yes
Atha\AT3G53860
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919050Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00008497
Drosophila simulans
Dsim\GD17628
Drosophila sechellia
Dsec\GM12214
Drosophila erecta
Dere\GG11995
Drosophila yakuba
Dyak\GE10420
Drosophila ananassae
Dana\GF16322
Drosophila pseudoobscura pseudoobscura
Dpse\GA14774
Drosophila persimilis
Dper\GL13478
Drosophila willistoni
Dwil\GK11447
Drosophila virilis
Dvir\GJ14216
Drosophila mojavensis
Dmoj\GI22340
Drosophila grimshawi
Dgri\GH13995
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502T9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024892
Glossina morsitans
Tsetse fly
Gmor\GMOY001243
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11943
Mayetiola destructor
Hessian fly
Mdes\Mdes008750
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL004832
Anopheles darlingi
American malaria mosquito
Adar\ADAC007924
Anopheles gambiae
Malaria mosquito
Agam\AGAP006680
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ012916
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ018194
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0ABO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009773
Danaus plexippus
Monarch butterfly
Dple\DPOGS213859-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL007130
Apis florea
Little honeybee
Aflo\2384582580
Apis mellifera
Western honey bee
Amel\GB49040
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP16521
Bombus terrestris
Buff-tailed bumblebee
Bter\15209115
Linepithema humile
Argentine ant
Lhum\LH15237
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0011c5
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG005240
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_05252
Tribolium castaneum
Red flour beetle
Tcas\TC033639
Pediculus humanus
Human body louse
Phum\PHUM354850
Rhodnius prolixus
Kissing bug
Rpro\RPRC001813
Cimex lectularius
Bed bug
Clec\CLEC006982
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009160
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03806
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X02U2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Isca\ISCW000480
Stegodyphus mimosarum
African social velvet spider
Smim\KFM66043.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM76214.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur20g02930
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_326156
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04C6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ino80d
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_004393gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000012575
Gallus gallus
Domestic chicken
Ggal\INO80D
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000028597
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    KANSL2; KAT8 regulatory NSL complex subunit 2
    13 of 15
    615488
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map

        3-99

        Cytogenetic map
        99C4-99C4
        Sequence location
        3R:29,854,695..29,857,491 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        99C4-99C4
        Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-99

        (FlyBase, 2016)

        3-97.0

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (10)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (20)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: dgt1 CG18041

          (Goshima et al., 2007)
          Source for database merge of
          Additional comments
          Other Comments

          RNAi screen using dsRNA made from templates generated with primers directed against this gene results in decreased γ-tubulin staining of the centrosome and spindle and the formation of an aberrantly long spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype is not observed without Cdc27 dsRNA.

          (Goshima et al., 2007)

          Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

          (Nybakken et al., 2005)
          Origin and Etymology
          Discoverer
          Etymology

          Named "dim γ-tubulin 1" for the decreased γ-tubulin staining of the spindle pole following treatment of S2 cells with dgt1 dsRNA.

          (Goshima et al., 2007)
          Identification
          External Crossreferences and Linkouts ( 41 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (6)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (42)