FlyBase Gene Report: Dmel\RpS8

General Information

Symbol

Dmel\RpS8

Species

D. melanogaster

Name

Ribosomal protein S8

Annotation Symbol

CG7808

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039713

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Ribosomal protein S8 (RpS8) encodes a structural constituent of ribosomes that is involved in larval lymph gland hemopoiesis. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

anon- EST:Liang-1.56 , S8

Key Links

Genomic Location

Cytogenetic map

99C4-99C4

Sequence location

3R:29,861,582..29,863,902 [+]

Recombination map

3-99

RefSeq locus

NT_033777 REGION:29861582..29863902

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

inferred from direct assay

(Anger et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables structural constituent of ribosome

inferred from sequence or structural similarity

(Liang and Biggin, 1998)

inferred from biological aspect of ancestor with PANTHER:PTN000044490

(assigned by GO_Central )

(Gaudet et al., 2011)

traceable author statement

(Marygold et al., 2007)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in larval lymph gland hemopoiesis

inferred from mutant phenotype

(Mondal et al., 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in cytoplasmic translation

traceable author statement

(Marygold et al., 2007)

inferred by curator from GO:0022626

(Anger et al., 2013, Alonso and Santaren, 2006)

involved_in maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

inferred from biological aspect of ancestor with PANTHER:PTN000044490

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in translation

inferred from electronic annotation with InterPro:IPR001047, InterPro:IPR018283

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Liang and Biggin, 1998)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of cytosolic small ribosomal subunit

traceable author statement

(Marygold et al., 2007)

inferred from biological aspect of ancestor with PANTHER:PTN000044490

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Liang and Biggin, 1998)

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS

Protein Family (UniProt)

Belongs to the eukaryotic ribosomal protein eS8 family. (Q8MLY8)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Ribosomal protein S8 (RpS8) encodes a structural constituent of ribosomes that is involved in larval lymph gland hemopoiesis. [Date last reviewed: 2019-07-11]

Gene Group (FlyBase)

CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -: Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RpS8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8MLY8)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.42

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RpS8-RC

FBtr0085489

NM_143483

1045

208

RpS8-RE

FBtr0310274

NM_001260433

756

208

RpS8-RF

FBtr0346158

NM_001300667

726

208

RpS8-RG

FBtr0346159

NM_001300668

931

208

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RpS8-PC

FBpp0099686

23.8

208

11.16

FBpp0301957

23.8

208

11.16

FBpp0311986

23.8

208

11.16

FBpp0311987

23.8

208

11.16

Q8MLY8

NP_001287597

AHN57596

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

208 aa isoforms: RpS8-PC, RpS8-PE, RpS8-PF, RpS8-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosolic ribosome

inferred from direct assay

(Anger et al., 2013)

inferred from high throughput direct assay

(Alonso and Santaren, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RpS8

View exonic expression by tissue for Dmel\RpS8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039713

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43532/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039713

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039713

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039713

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7808

Flygut - An atlas of the Drosophila adult midgut

FBgn0039713

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RpS8

Transgenic constructs containing regulatory region of RpS8

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

RpS8^GD12288

lethal, with Scer\GAL4^pnr-MD237

RpS8^GD12288

lethal | pupal stage, with Scer\GAL4^pnr-MD237

RpS8^GD12288

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

RpS8^GD12288

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RpS8^GD12288

viable, with Scer\GAL4^tin.CΔ4

RpS8^GD12288

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage

RpS8^GD12288

Phenotype manifest in

Allele

embryonic/larval lymph gland, with Scer\GAL4^Hml.Δ

RpS8^KK108882

ganglion mother cell | larval stage

RpS8^GD12288

larval neuroblast | larval stage

RpS8^GD12288

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\RPS8

14 of 15

Yes

Hsap\NSA2

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Rps8

8 of 15

Yes

Mmus\Gm15501

7 of 15

Yes

Mmus\Gm11353

2 of 15

Yes

Mmus\Nsa2

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Rps8

10 of 13

Yes

Rnor\LOC297756

8 of 13

Yes

Rnor\LOC100363452

3 of 13

Yes

Rnor\RGD1566369

3 of 13

Yes

Rnor\Nsa2

1 of 13

Xenopus tropicalis (Western clawed frog) (2)

Xtro\rps8

9 of 12

Yes

Xtro\nsa2

1 of 12

Danio rerio (Zebrafish) (3)

Drer\rps8b

14 of 15

Yes

Drer\rps8a

13 of 15

Yes

Drer\nsa2

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\rps-8

13 of 15

Yes

Cele\W09C5.1

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT5G20290

8 of 9

Yes

Atha\AT5G59240

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\RPS8B

13 of 15

Yes

Scer\RPS8A

8 of 15

Yes

Scer\NSA2

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\rps801

11 of 12

Yes

Spom\rps802

11 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RPS8; ribosomal protein S8

14 of 15

600357

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RpS8 - Mer

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

RpS8 - Rack1

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL10

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL10Ab

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL13A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL14

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL18A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL19

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL22

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL23A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL28

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL30

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL31

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL32

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL34a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL35

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL35A

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL36

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL36A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL37A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL37a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL38

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL39

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL40

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL41

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpL5

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL7A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL8

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpL9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpLP0

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpLP1

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpLP2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS10a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS11

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS12

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS13

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS14a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS14b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS15

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS15Aa

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS16

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS17

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS18

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS19a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS20

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS21

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS23

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS24

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS25

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS26

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS27

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS27A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS28b

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS29

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS3

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS30

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - RpS3A

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS4

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS5a

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS6

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS7

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - RpS9

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - eEF2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - kra

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Wang et al., 2006)

RpS8 - mats

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

RpS8 - sta

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RpS8 - vig2

cosedimentation, Identification by mass spectrometry, electron microscopy

(Anger et al., 2013)

RNA-protein

Physical Interaction

Assay

References

RpS8 - 18SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - 28SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - 2SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - 5.8SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

RpS8 - 5SrRNA

cosedimentation, electron microscopy

(Anger et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68391

DroID - A comprehensive database of gene and protein interactions.

FBgn0039713

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0039713

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-99

Cytogenetic map

99C4-99C4

Sequence location

3R:29,861,582..29,863,902 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99C4-99C4

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Distal to Df(3R)01215 and quite likely lies proximal to Df(3R)X3F.

(Cook, 2006.1.31)

Experimentally Determined Recombination Data

Location

3-99

(FlyBase, 2016)

3-97.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

22076

y¹ w^67c23; P{Mae-UAS.6.11}RpS8^DP01446

FlyORF

F001303

M{UAS-RpS8.ORF}ZH-86Fb

F001175

M{UAS-RpS8.ORF.3xHA}ZH-86Fb

VDRC

v106835

P{KK108882}VIE-260B

v35277

w¹¹¹⁸; P{GD12288}v35277

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (806)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039713

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039713

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43532

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: CG7808 anon- EST:fe1A2

(Bayraktaroglu, 2000.11.2)

Source for merge of: RpS8 anon- EST:Liang-1.56

(FlyBase, 1996-)

Source for merge of: CG7808 BcDNA:RH06886

(FlyBase, 1996-)

Additional comments

"RpS8" likely corresponds to "M(3)99B".

(Marygold et al., 2007)

May correspond to M(3)99B.

(Cook, 2006.1.31)

Source for merge of RpS8 anon- EST:Liang-1.56 was sequence comparison ( date:040623 ).

(FlyBase, 1996-)

Source for merge of CG7808 BcDNA:RH06886 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Other Comments

Haploinsufficient locus.

(Cook et al., 2012)

Likely Minute gene.

(Marygold et al., 2007)

RpS8 lies in a gap in deletion coverage.

(Marygold et al., 2007)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Identified with: SD17528 (BDGP-DGC) <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

(BDGP Project Members, 2000-)

EST SD17528 is chimeric; the 5' portion matches part of AE on the opposite strand to Glt, the 3' portion corresponds to part of RpS8.

(FlyBase, 1996-)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 62 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43532

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8MLY8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8MLY8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4K6N1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8MLY8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039713

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039713

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43532/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039713

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039713

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039713

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7808

Flygut - An atlas of the Drosophila adult midgut

FBgn0039713

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0039713

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43532

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7808

MARRVEL_MODEL - MARRVEL (model organism gene)

43532

modMine - A data warehouse for the modENCODE project

FBgn0039713

Linkouts

BioGRID - A database of protein and genetic interactions.

68391

DroID - A comprehensive database of gene and protein interactions.

FBgn0039713

DRSC - Results frm RNAi screens

FBgn0039713

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039713

FlyMine - An integrated database for Drosophila genomics

FBgn0039713

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ribosome

MIST (protein-protein) - An integrated Molecular Interaction Database

RpS8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

BcDNA:RH06886

BcDNA:SD17528

CG7808

(Kolkhof et al., 2017, Rosa-Ferreira et al., 2015, Fukuoh et al., 2014, Sing et al., 2014, Hoffmann et al., 2013, Schertel et al., 2013, Cammarato et al., 2011, Müller et al., 2010, Keleman et al., 2009.8.5, Marygold et al., 2007, Stuart et al., 2007, Cermelli et al., 2006, Huang et al., 2005, Ashburner, 2004.6.30, Boutros et al., 2004, Bolshakov et al., 2002)

RPS8

(Wang et al., 2006)

Rp S8

(Alonso and Santaren, 2006)

Rps8

(Sims et al., 2009)

(Carpenter et al., 2009, Brogna et al., 2002)

S8e

(Anger et al., 2013)

anon-EST:Liang-1.56

(Ashburner, 2004.6.30)

anon-EST:fe1A2

chr3R:25688477..25688608

(Will et al., 2013)

clone 1.56

(Liang and Biggin, 1998)

Name Synonyms

Ribosomal protein S8

(Hoffmann et al., 2013, Cammarato et al., 2011, Ashburner and Marygold, 2004.7.2)

anon-fast-evolving-1A2

Secondary FlyBase IDs

FBgn0025277
FBgn0025982
FBgn0062869
FBgn0062967

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (87)

The following external sites may use different assemblies or annotations than FlyBase.

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