• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Mgat2

General Information
Symbol	Dmel\Mgat2	Species	D. melanogaster
Name	Mgat2	Annotation symbol	CG7921
Feature type	protein_coding_gene	FlyBase ID	FBgn0039738
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99D1-99D2	Sequence location	3R:25,841,227..25,847,980 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mgat2 is referred to in FlyBase by the symbol Dmel\Mgat2 (CG7921, FBgn0039738). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: acetylglucosaminyltransferase activity. There is experimental evidence that it is involved in the biological process: protein N-linked glycosylation. 6 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: N-acetylglucosaminyltransferase II. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult hindgut, adult heart, adult salivary gland, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1633061_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Mgat2. Gene sequence location is 3R:25841227..25847980.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN27563 HFA16347
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99D1-99D2   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99D4-99D5     (Tan, 2001.9.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mgat2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mgat2-RA FBtr0085602  NM_143506   3376   608 Mgat2-RB FBtr0100418  NM_001014684   2700   488
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mgat2-PA   FBpp0084967   69.1   608   9.34     NP_651763   AAF56991 Mgat2-PB   FBpp0099836   56.3   488   9.08     NP_001014684   AAX53005
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • N-acetylglucosaminyltransferase II (IPR007754)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698936   AA802964   AE014297 AAF56991   AE014297 AAX53005   AB176553 BAD54757   AI064116   AI108605   AI114298   AI404468   AI542626   AI543924   AJ303073 CAC83074   AJ428392 CAD21427   AW940271   AY047566 AAK77298   AY055120 AAL17663   BI229352   BI229373   BI619346   BI625690   BT124758   CK658178   CL528063   CO179150   CO267598   CO278283   CO321991   CO338907   CZ467121   CZ479009   CZ479074   EC069837   EC072608   EC073167   EC085872   EC206040   EC214340   EC221761   EC254233   EL869675   EL869676   EL869677   EL869678   EL869679   EL869722   EL869723
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q710C1 Q711U4 Q95UE9 Q961U0 Q59DT4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0039738    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0039738 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0039738 expression level Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr  (459) embryo 04-06hr  (441) embryo 06-08hr  (672) embryo 08-10hr  (788) embryo 10-12hr  (1025) embryo 12-14hr  (980) embryo 14-16hr  (1630) embryo 16-18hr  (1436) embryo 18-20hr  (1169) embryo 20-22hr  (674) embryo 22-24hr  (761) larva L1  (719) larva L2  (307) larva L3 12hr old  (248) larva L3 puffstage 1-2  (340) larva L3 puffstage 3-6  (496) larva L3 puffstage 7-9  (788) white prepupae new  (788) white prepupae 12hr  (858) white prepupae 24hr  (1154) pupae 2d postWPP  (1418) pupae 3d postWPP  (1544) pupae 4d postWPP  (1418) adult male 01day  (1282) adult male 05day  (1687) adult male 30day  (1539) adult female 01day  (725) adult female 05day  (271) adult female 30day  (224) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr  (1630) embryo 16-18hr  1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP  1418 pupae 3d postWPP  (1544) pupae 4d postWPP  1418 adult male 01day    1282 adult male 05day  (1687) adult male 30day  (1539) adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0039738 expression level Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr  (459) embryo 04-06hr  (441) embryo 06-08hr  (672) embryo 08-10hr  (788) embryo 10-12hr  (1025) embryo 12-14hr  (980) embryo 14-16hr  (1630) embryo 16-18hr  (1436) embryo 18-20hr  (1169) embryo 20-22hr  (674) embryo 22-24hr  (761) larva L1  (719) larva L2  (307) larva L3 12hr old  (248) larva L3 puffstage 1-2  (340) larva L3 puffstage 3-6  (496) larva L3 puffstage 7-9  (788) white prepupae new  (788) white prepupae 12hr  (858) white prepupae 24hr  (1154) pupae 2d postWPP  (1418) pupae 3d postWPP  (1544) pupae 4d postWPP  (1418) adult male 01day  (1282) adult male 05day  (1687) adult male 30day  (1539) adult female 01day  (725) adult female 05day  (271) adult female 30day  (224) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr  1630 embryo 16-18hr  1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP  1418 pupae 3d postWPP  1544 pupae 4d postWPP  1418 adult male 01day    1282 adult male 05day  1687 adult male 30day  1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    73 embryo 02-04hr    459 embryo 04-06hr    441 embryo 06-08hr    672 embryo 08-10hr    788 embryo 10-12hr    1025 embryo 12-14hr    980 embryo 14-16hr    1630 embryo 16-18hr    1436 embryo 18-20hr    1169 embryo 20-22hr    674 embryo 22-24hr    761 larva L1    719 larva L2    307 larva L3 12hr old    248 larva L3 puffstage 1-2    340 larva L3 puffstage 3-6    496 larva L3 puffstage 7-9    788 white prepupae new    788 white prepupae 12hr    858 white prepupae 24hr    1154 pupae 2d postWPP    1418 pupae 3d postWPP    1544 pupae 4d postWPP    1418 adult male 01day    1282 adult male 05day    1687 adult male 30day    1539 adult female 01day    725 adult female 05day    271 adult female 30day    224 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0039738    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0039738 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult hindgut, adult heart, adult salivary gland, adult testis, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0039738 expression level Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain  (654) Adult Thoracic-Abdominal Ganglion  (936.7) Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0039738 expression level Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain  654 Adult Thoracic-Abdominal Ganglion  (936.7) Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut no informative data Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea    86.95 Larval Carcass no informative data Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut no informative data Adult Hindgut    256.5 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary no informative data Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland no informative data Larval Trachea     Larval Carcass no informative data Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut no informative data Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary no informative data Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    182.3   NA  Eye    181.225   NA  Brain    654   241.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    936.7   NA  Crop    99.4   no informative data  Midgut    no informative data   84  Hindgut    256.5   94.7  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    418.6   NA  Heart    174.225   86.95  Trachea    NA   NA  VirginFemale Spermatheca    40.8   NA  InseminatedFemale Spermatheca    46.3   NA  Ovary    no informative data   NA  Testis    116.4   NA  Male Accessory Gland    64.2   no informative data  Carcass    196.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_24
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039738 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7921-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mgat2 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Mgat2EY07798 viable, with Scer\GAL4pnr-MD237 Mgat2GD377 Sterility Allele fertile Mgat2EY07798 Phenotype manifest in Allele
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Mgat2 Class Mutagen Stocks Known lesion Mgat2c01386 piggyBac transposase 1 -- Mgat2DP01278 1 -- Mgat2e01200 piggyBac transposase 1 -- Mgat2e01270 piggyBac transposase 1 -- Mgat2EY07798 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mgat2 Class Mutagen Stocks Known lesion Mgat2GD377 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD377} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Mgat2EY07798 NA P{Mae-UAS.6.11}Mgat2DP01278 NA miscellaneous insertions PBac{PB}Mgat2c01386 NA PBac{RB}Mgat2e01200 NA PBac{RB}Mgat2e01270 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function
CV term Evidence References
acetylglucosaminyltransferase activity inferred from direct assay (Ichimiya et al., 2004)
Biological Process
CV term Evidence References
protein N-linked glycosylation inferred from direct assay (Ichimiya et al., 2004)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity inferred from sequence or structural similarity with UniProtKB:Q09326 (FlyBase, 1992-)
Biological Process
CV term Evidence References
oligosaccharide biosynthetic process inferred from electronic annotation with InterPro:IPR007754 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
Golgi stack inferred from electronic annotation with InterPro:IPR007754 (FlyBase Curators et al., 2004-) integral to membrane inferred from electronic annotation with InterPro:IPR007754 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mgat2 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0039738
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16208 Dere\GG11975 Dgri\GH22139 Dmoj\GI21962 Dper\GL13486 Dpse\GA26865 Dsec\GM12193 Dsim\GD17443 Dvir\GJ14322 Dwil\GK13428 Dyak\GE23425
Curated drosophilid orthologs	Dpse\GA20689
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005949-PA Aedes aegypti (mosquito) AGAP004397-PA Anopheles gambiae (mosquito) XP_001122208.1 Apis mellifera (honey bee) At2g05320.1 Arabidopsis thaliana (wall cress) BGIBMGA001608-PA Bombyx mori (silk worm) CN06416 Caenorhabditis brenneri (small nematode) CBP32738 Caenorhabditis briggsae (nematode) CE26613 Caenorhabditis elegans (worm) JA08748 Caenorhabditis japonica (nematode) CPIJ003883 Culex pipens (mosquito) RP34404 Caenorhabditis remanei (nematode) ENSDARP00000068744 Danio rerio (zebra fish) ENSGACP00000016998 Gymnopilus aculeatus (mushroom) 108438 Lottia gigantea (owl limpet) 11959 Nematostella vectensis (starlet anemone) ENSOANP00000022065 Ornithorynchus anatinus (platypus) ENSORLP00000019798 Oryzias latipes (japanese killifish) LOC_Os02g55470.1 Oryza sativa (asian rice) PHUM003451-PA Pediculus humanus (human body louse) 113818 Physcomitrella patens (moss) 247036 Populus trichocarpa (black cottonwood) 4885034 Sorghum bicolor (sorghum) Smp_089240 Schistosoma mansoni (trematode parasite) XP_969675.1 Tribolium castaneum (red flour beetle) ENSTNIP00000005016 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000020003 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13086 LH20521 ISCW022527 ISCW014205 FBgn0240610 FBgn0215436 FBgn0203738 FBgn0201536 FBgn0198468 FBgn0188997 FBgn0167130 JGI_V11_305651 FBgn0248237 FBgn0151091 FBgn0144691 FBgn0129600 FBgn0121198 FBgn0104267 FBgn0093230 CPIJ003884 CPIJ003883 BGIBMGA001608 ACYPI001004 GB10457 AGAP004397 AAEL005949 PB21183 PHUM003200 GLEAN_01867
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	16419 y1 w67c23; P{EPgy2}Mgat2EY07798 21838 y1 w67c23; P{Mae-UAS.6.11}Mgat2DP01278
Harvard	- PBac{PB}Mgat2c01386 - PBac{RB}Mgat2e01200 - PBac{RB}Mgat2e01270
VDRC	v1380 w1118; P{GD377}v1380 v1381 w1118; P{GD377}v1381
Genomic Clones ( 1 )
	BACR07A18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 32 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH07804
Other clones	MIP20596
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04380 GH11105 GH24206 GM06662 HL05976 LD45612 LP14904 RE27119 RE27143 RH50366 RH66159 SD09048
Other clones	17414 33193 38199 60888 119616 214311.21 CK01.8D.G4 CK01.74C.F11 EK058622 EK129632 EK150964 EK186944 EP00160 IP01773 IP01774 IP01775 IP01776 IP01829
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039738 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43563
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mgat2 CG7921 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of Mgat2 CG7921 was sequence comparison (date:011104). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143506 NM_001014684 RefSeq (Proteins) • NP_651763 NP_001014684 DDBJ / EMBL / GenBank • AAF56991 AAF56991.2 AAF56991.3 AAK77298 AAL17663 AAX53005 AB176553 AI108605 AJ303073 AJ428392 AW940271 AY047566 AY055120 BAD54757 CAC83074 CAD21427 CL528063 CZ467121 CZ479009 CZ479074 Entrez Gene - A searchable database of RefSeq genes. • 43563 UniProtKB/TrEMBL • Q59DT4 Q95UE9 Q710C1 Q711U4 Q961U0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • N-acetylglucosaminyltransferase II (IPR007754)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0039738 DRSC - Results from RNAi screens. • FBgn0039738 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039738 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7921-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039738 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43563 InParanoid A subset of ortholog calls from InParanoid. • AAEL005949-PA Aedes aegypti (mosquito) AGAP004397-PA Anopheles gambiae (mosquito) XP_001122208.1 Apis mellifera (honey bee) At2g05320.1 Arabidopsis thaliana (wall cress) BGIBMGA001608-PA Bombyx mori (silk worm) CN06416 Caenorhabditis brenneri (small nematode) CBP32738 Caenorhabditis briggsae (nematode) CE26613 Caenorhabditis elegans (worm) JA08748 Caenorhabditis japonica (nematode) CPIJ003883 Culex pipens (mosquito) RP34404 Caenorhabditis remanei (nematode) ENSDARP00000068744 Danio rerio (zebra fish) ENSGACP00000016998 Gymnopilus aculeatus (mushroom) 108438 Lottia gigantea (owl limpet) 11959 Nematostella vectensis (starlet anemone) ENSOANP00000022065 Ornithorynchus anatinus (platypus) ENSORLP00000019798 Oryzias latipes (japanese killifish) LOC_Os02g55470.1 Oryza sativa (asian rice) PHUM003451-PA Pediculus humanus (human body louse) 113818 Physcomitrella patens (moss) 247036 Populus trichocarpa (black cottonwood) 4885034 Sorghum bicolor (sorghum) Smp_089240 Schistosoma mansoni (trematode parasite) XP_969675.1 Tribolium castaneum (red flour beetle) ENSTNIP00000005016 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000020003 Xenopus tropicalis (frog) modMine - Data generated by the modENCODE project. • FBgn0039738 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13086 LH20521 ISCW022527 ISCW014205 FBgn0240610 FBgn0215436 FBgn0203738 FBgn0201536 FBgn0198468 FBgn0188997 FBgn0167130 JGI_V11_305651 FBgn0248237 FBgn0151091 FBgn0144691 FBgn0129600 FBgn0121198 FBgn0104267 FBgn0093230 CPIJ003884 CPIJ003883 BGIBMGA001608 ACYPI001004 GB10457 AGAP004397 AAEL005949 PB21183 PHUM003200 GLEAN_01867
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG7921 (Halfon et al., 2002, ) dMGAT2 (Ichimiya et al., 2004) GlcNAcT2 (Baas et al., 2011) GlcNAc-TII (Fabini and Wilson, 2001) Mgat2 (Stark et al., 2005)
Name Synonym	Mgat2
Secondary FlyBase IDs
References ( 19 )
Research paper	Baas et al., 2011, Development 138(3): 553--563 Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo. [FBrf0212690] Stark et al., 2005, Cell 123(6): 1133--1146 Animal MicroRNAs confer robustness to gene expression and have a significant impact on 3'UTR evolution. [FBrf0189926] Ichimiya et al., 2004, J. Biol. Chem. 279(41): 42638--42647 The twisted abdomen phenotype of Drosophila POMT1 and POMT2 mutants coincides with their heterophilic protein O-mannosyltransferase activity. [FBrf0179828] Inlow and Restifo, 2004, Genetics 166(2): 835--881 Molecular and comparative genetics of mental retardation. [FBrf0174714] Tsitilou and Grammenoudi, 2003, Biochem. biophys. Res. Commun. 312(4): 1372--1376 Evidence for alternative splicing and developmental regulation of the Drosophila melanogaster Mgat2 (N-acetylglucosaminyltransferase II) gene. [FBrf0167336] Halfon et al., 2002, Genome Res. 12(7): 1019--1028 Computation-based discovery of related transcriptional regulatory modules and motifs using an experimentally validated combinatorial model. [FBrf0151337] Fabini and Wilson, 2001, D. I. S. 84: 122--129 Glycosyltransferases in Drosophila melanogaster. [FBrf0150727]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Levis, 2009.8.13, Release 5 sequence localizations for HP and DP lines. Release 5 sequence localizations for HP and DP lines. [FBrf0208533] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Tan, 2001.9.10, GenBank/EMBL/DDBJ: AY055120 Drosophila melanogaster UDP-GlcNAc:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II (mgat2) mRNA, complete cds. [FBrf0140753]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177] BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Curated genome annotation	Zhu, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126704]