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Gene Dmel\Mgat2

General Information
Symbol	Dmel\Mgat2	Species	D. melanogaster
Name	Mgat2	Annotation symbol	CG7921
Feature type	protein_coding_gene	FlyBase ID	FBgn0039738
Gene Model Status	Current	Stock availability	7 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99D1-99D2	Sequence location	3R:25,841,235..25,847,943 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mgat2 is referred to in FlyBase by the symbol Dmel\Mgat2 (CG7921, FBgn0039738). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: acetylglucosaminyltransferase activity. There is experimental evidence that it is involved in the biological process: protein N-linked glycosylation. 7 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: N-acetylglucosaminyltransferase II. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult hindgut, adult heart, adult salivary gland, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1633061_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Mgat2. Gene sequence location is 3R:25841235..25847943.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99D1-99D2   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99D4-99D5     (Tan, 2001.9.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mgat2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mgat2-RA FBtr0085602  NM_143506   3331   608 Mgat2-RB FBtr0100418  NM_001014684   2672   488 Mgat2-RC FBtr0334705  NM_001276167   3034   509
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mgat2-PA   FBpp0084967   69.1   608   9.34     NP_651763   AAF56991 Mgat2-PB   FBpp0099836   56.3   488   9.08     NP_001014684   AAX53005 Mgat2-PC   FBpp0306759   58.8   509   9.41     NP_001263096   AGB96475
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • N-acetylglucosaminyltransferase II (IPR007754)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698936   AA802964   AE014297 AAF56991   AE014297 AAX53005   AB176553 BAD54757   AE014297 AGB96475   AI064116   AI108605   AI114298   AI404468   AI542626   AI543924   AJ303073 CAC83074   AJ428392 CAD21427   AW940271   AY047566 AAK77298   AY055120 AAL17663   BI229352   BI229373   BI619346   BI625690   BT124758   CK658178   CL528063   CO179150   CO267598   CO278283   CO321991   CO338907   CZ467121   CZ479009   CZ479074   EC069837   EC072608   EC073167   EC085872   EC206040   EC214340   EC221761   EC254233   EL869675   EL869676   EL869677   EL869678   EL869679   EL869722   EL869723
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q961U0 Q59DT4 Q95UE9 Q710C1 Q711U4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) garland cell primordium   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval garland cell   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Golgi medial cisterna inferred from direct assay (Yamamoto-Hino et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult hindgut, adult heart, adult salivary gland, adult testis, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain  (654) Adult Thoracic-Abdominal Ganglion  (936.7) Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain  654 Adult Thoracic-Abdominal Ganglion  (936.7) Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    241.225 Larval Midgut    28.3 Larval Hindgut    84 Larval Malpighian Tubules    94.7 Larval Fat Body    52 Larval Salivary Gland    44.1 Larval Trachea    86.95 Larval Carcass    56.825 Adult Head    182.3 Adult Eye    181.225 Adult Brain    654 Adult Thoracic-Abdominal Ganglion    936.7 Adult Crop    99.4 Adult Midgut    33.3 Adult Hindgut    256.5 Adult Malpighian Tubules    42.6 Adult Fat Body    52.4 Adult Salivary Gland    418.6 Adult Heart    174.225 Adult VirginFemale Spermatheca    40.8 Adult InseminatedFemale Spermatheca    46.3 Adult Ovary    15.3 Adult Testis    116.4 Adult Male Accessory Gland    64.2 Adult Carcass    196.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    182.3   NA  Eye    181.225   NA  Brain    654   241.225  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    936.7   NA  Crop    99.4   no informative data  Midgut    no informative data   84  Hindgut    256.5   94.7  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    418.6   NA  Heart    174.225   86.95  Trachea    NA   NA  VirginFemale Spermatheca    40.8   NA  InseminatedFemale Spermatheca    46.3   NA  Ovary    no informative data   NA  Testis    116.4   NA  Male Accessory Gland    64.2   no informative data  Carcass    196.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3  (32) central nervous system, pupae P8  (60) head, virgin 1-day female  (33) head, virgin 4-day female  (29) head, virgin 20-day female    23 head, mated 1-day female  (29) head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male  (54) head, mated 4-day male  (43) head, mated 20-day male  (42) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3  32 central nervous system, pupae P8  (60) head, virgin 1-day female  (33) head, virgin 4-day female  29 head, virgin 20-day female    23 head, mated 1-day female  29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male  (54) head, mated 4-day male  (43) head, mated 20-day male  (42) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    32 central nervous system, pupae P8    60 head, virgin 1-day female    33 head, virgin 4-day female    29 head, virgin 20-day female    23 head, mated 1-day female    29 head, mated 4-day female    23 head, mated 20-day female    19 head, mated 1-day male    54 head, mated 4-day male    43 head, mated 20-day male    42 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    4 digestive system, 4-day adult    3 digestive system, 20-day adult    5 fat body, larvae L3 wandering    4 fat body, white prepupae    7 fat body, pupae P8    21 carcass, larvae L3 wandering    11 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    12 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    22 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages, during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    6 embryo 04-06hr    6 embryo 06-08hr    9 embryo 08-10hr    10 embryo 10-12hr    13 embryo 12-14hr    13 embryo 14-16hr    21 embryo 16-18hr    19 embryo 18-20hr    15 embryo 20-22hr    9 embryo 22-24hr    10 larva L1    9 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    11 white prepupae 24hr    15 pupae 2d postWPP    19 pupae 3d postWPP    20 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    22 adult male 30day    20 adult female 01day    10 adult female 05day    4 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32  (35) haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32  (35) haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    11 Schneider line 2 Sg4    4 embryonic 1182-4H    19 embryonic GM2    14 embryonic Kc167    6 embryonic S1    15 embryonic S3    4 leg disc CME L1    3 wing disc CME-W2    6 wing disc ML-DmD8    7 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    8 wing disc ML-DmD32    35 haltere disc ML-DmD17-c3    8 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    10 tumorous blood cells mbn2    4 ovary fGS/OSS    11 ovary OSC    6 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    8 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    8 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    6 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    11 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_14
External Data & Images
Linkouts	BDGP expression data • GH07804 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039738 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7921-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mgat2 Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Mgat2EY07798 viable, with Scer\GAL4pnr-MD237 Mgat2GD377 Sterility Allele fertile Mgat2EY07798 Phenotype manifest in Allele
Classical Alleles ( 5 )
For All Classical Alleles Show
Allele of Mgat2 Class Mutagen Stocks Known lesion Mgat2c01386 piggyBac transposase 1 -- Mgat2DP01278 1 -- Mgat2e01200 piggyBac transposase 1 -- Mgat2e01270 piggyBac transposase 1 -- Mgat2EY07798 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mgat2 Class Mutagen Stocks Known lesion Mgat2GD377 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Mgat2Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD377} NA P{UAS-Mgat2.HA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Mgat2EY07798 NA P{Mae-UAS.6.11}Mgat2DP01278 NA miscellaneous insertions PBac{PB}Mgat2c01386 NA PBac{RB}Mgat2e01200 NA PBac{RB}Mgat2e01270 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
acetylglucosaminyltransferase activity inferred from direct assay (Ichimiya et al., 2004)
Biological Process
CV term Evidence References
protein N-linked glycosylation inferred from direct assay (Ichimiya et al., 2004)
Cellular Component
CV term Evidence References
Golgi medial cisterna inferred from direct assay (Yamamoto-Hino et al., 2012)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity inferred from sequence or structural similarity with UniProtKB:Q09326 (FlyBase, 1992-)
Biological Process
CV term Evidence References
oligosaccharide biosynthetic process inferred from electronic annotation with InterPro:IPR007754 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR007754 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mgat2 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0039738
Orthologs
OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6SJ60D Dipteran inclusive ortholog search EOG6T1HVR Insect inclusive ortholog search EOG6ZS7ZD Arthropod inclusive ortholog search EOG6PZGNT Metazoa inclusive ortholog search EOG6X6R2P
Orthologs in Drosophila Species (EOG6SJ60D)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mgat2     Drosophila simulans   Dsim\GD17443 Y   Drosophila sechellia   Dsec\GM12193 Y   Drosophila erecta   Dere\GG11975 Y   Drosophila yakuba   Dyak\GE23425 Y   Drosophila ananassae   Dana\GF16208 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26865 Y   Drosophila persimilis   Dper\GL13486 Y   Drosophila willistoni   Dwil\GK13428 Y   Drosophila virilis   Dvir\GJ14322 Y   Drosophila mojavensis   Dmoj\GI21962 Y   Drosophila grimshawi   Dgri\GH22139 Y
Orthologs in non-Drosophila Dipterans (EOG6T1HVR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005949   Anopheles gambiae Malaria mosquito  Agam\AGAP004397   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003884   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ003883
Orthologs in non-Dipteran Insects (EOG6ZS7ZD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50232   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006714   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19177   Atta cephalotes Leafcutter ant  Acep\ACEP18099   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20346   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16851   Linepithema humile Argentine ant  Lhum\LH20521   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21183   Solenopsis invicta Red fire ant  Sinv\SINV11410   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001004   Acyrthosiphon pisum Pea aphid  Apim\ACYPI064239   Bombyx mori Silkmoth  Bmor\BGIBMGA001608   Pediculus humanus Human body louse  Phum\PHUM003200   Tribolium castaneum Red flour beetle  Tcas\TC001867
Orthologs in non-Insect Arthropods (EOG6PZGNT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_305651   Ixodes scapularis Deer tick  Isca\ISCW022527   Ixodes scapularis Deer tick  Isca\ISCW014205
Orthologs in non-Arthropod Metazoa (EOG6X6R2P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\gly-20   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003990gn   Danio rerio Zebrafish  Drer\CH73-301C19.1   Danio rerio Zebrafish  Drer\ENSDARG00000091598   Xenopus tropicalis Western clawed frog  Xtro\mgat2   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000025754   Mus musculus House mouse  Mmus\Mgat2   Rattus norvegicus Norway rat  Rnor\Mgat2   Homo sapiens Human  Hsap\MGAT2
Human Orthologs (1)
Gene OMIM HGNC Hsap\MGAT2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17443 Drosophila sechellia Dsec\GM12193 Drosophila erecta Dere\GG11975 Drosophila yakuba Dyak\GE23425 Drosophila ananassae Dana\GF16208 Drosophila pseudoobscura pseudoobscura Dpse\GA26865 Drosophila persimilis Dper\GL13486 Drosophila willistoni Dwil\GK13428 Drosophila virilis Dvir\GJ14322 Drosophila mojavensis Dmoj\GI21962 Drosophila grimshawi Dgri\GH22139
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	16419 y1 w67c23; P{EPgy2}Mgat2EY07798 21838 y1 w67c23; P{Mae-UAS.6.11}Mgat2DP01278
Harvard	- PBac{PB}Mgat2c01386 - PBac{RB}Mgat2e01200 - PBac{RB}Mgat2e01270
VDRC	v1380 w1118; P{GD377}v1380 v1381 w1118; P{GD377}v1381
Genomic Clones ( 1 )
	BACR07A18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 32 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH07804
Other clones	MIP20596
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH04380 GH11105 GH24206 GM06662 HL05976 LD45612 LP14904 RE27119 RE27143 RH50366 RH66159 SD09048
Other clones	17414 33193 38199 60888 119616 214311.21 CK01.8D.G4 CK01.74C.F11 EK058622 EK129632 EK150964 EK186944 EP00160 IP01773 IP01774 IP01775 IP01776 IP01829
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039738 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43563
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mgat2 CG7921 (FlyBase, 1996-)
Source for database merge of
Additional comments	Source for identity of Mgat2 CG7921 was sequence comparison (date:011104). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143506 NM_001014684 NM_001276167 RefSeq (Proteins) • NP_651763 NP_001014684 NP_001263096 DDBJ / EMBL / GenBank • AAF56991 AAF56991.2 AAF56991.3 AAK77298 AAL17663 AAX53005 AB176553 AGB96475 AI108605 AJ303073 AJ428392 AW940271 AY047566 AY055120 BAD54757 CAC83074 CAD21427 CL528063 CZ467121 CZ479009 CZ479074 Entrez Gene - A searchable database of RefSeq genes. • 43563 UniProtKB/TrEMBL • Q59DT4 Q95UE9 Q710C1 Q711U4 Q961U0
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • N-acetylglucosaminyltransferase II (IPR007754) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH07804
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0039738 DRSC - Results from RNAi screens. • FBgn0039738 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039738 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7921-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039738 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43563 modMine - Data generated by the modENCODE project. • FBgn0039738
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG7921 (Halfon et al., 2002, Yamamoto-Hino et al., 2012) dMGAT2 (Ichimiya et al., 2004) GlcNAcT2 (Baas et al., 2011) GlcNAc-TII (Fabini and Wilson, 2001) Mgat2 (Stark et al., 2005, Mortimer et al., 2012, Yamamoto-Hino et al., 2012)
Name Synonym	Mgat2
Secondary FlyBase IDs
References ( 23 )
Generate a list of	All references relating to this gene ( 23 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 3 )
	Mortimer et al., 2012, PLoS Pathog. 8(7): e1002819 Mgat1-dependent N-glycosylation of Membrane Components Primes Drosophila melanogaster Blood Cells for the Cellular Encapsulation Response. [FBrf0218977] Yamamoto-Hino et al., 2012, Cell Struct. Funct. 37(1): 55--63 Cisterna-specific Localization of Glycosylation-related Proteins to the Golgi Apparatus. [FBrf0217862] Baas et al., 2011, Development 138(3): 553--563 Sugar-free frosting, a homolog of SAD kinase, drives neural-specific glycan expression in the Drosophila embryo. [FBrf0212690] Show all research papers ...