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Gene Dmel\PH4αEFB

General Information
Symbol	Dmel\PH4αEFB	Species	D. melanogaster
Name	prolyl-4-hydroxylase-alpha EFB	Annotation symbol	CG31022
Feature type	protein_coding_gene	FlyBase ID	FBgn0039776
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	PH4alphaEFB
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	99F6-99F6	Sequence location	3R:26,291,772..26,311,521 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene prolyl-4-hydroxylase-alpha EFB is referred to in FlyBase by the symbol Dmel\PH4αEFB (CG31022, FBgn0039776). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: procollagen-proline 4-dioxygenase activity. Based on sequence similarity, it is predicted to be involved in the biological process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline. 13 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Oxoglutarate/iron-dependent dioxygenase; Prolyl 4-hydroxylase alpha-subunit, N-terminal; Prolyl 4-hydroxylase, alpha subunit; Tetratricopeptide-like helical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult heart, adult fat body, adult spermathecae, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval trachea, adult ovary, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628705_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of PH4alphaEFB. Gene sequence location is 3R:26291772..26311521.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 99F6-99F6   Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\PH4αEFB for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency junction(s) not annotated. Gene model reviewed during 5.44
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) PH4αEFB-RA FBtr0085650  NM_170492   2248   550
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein PH4αEFB-PA   FBpp0085012   63.1   550   5.16     NP_733371   AAF57053
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent dioxygenase (IPR005123) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Prolyl 4-hydroxylase alpha-subunit, N-terminal (IPR013547) Tetratricopeptide-like helical (IPR011990)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694968   AA802406   AE014297 AAF57053   AF495536 AAM18058   AI389448   AI402915   AI531161   AI531215   AI531830   AI531831   AI532280   AI532345   AI532350   AI533665   AI542093   AY052109 AAK93533   AY069737   BF489562   BG635585   BG636112   BG639597   BG640316   BG640350   BI215768   BI354119   BI365012   BI373090   BI375681   BI485792   BI486746   BI567010   BI627223   BI632443   BI632466   BI632591   BI632670   BI636202   BI636829   BI637517   BI637522   BI637755   BI639711   BI640738   BT031148   CO277589   CZ468145   CZ480599   CZ480600   EC071783   EL878949   EL878950
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VA69
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) plasmatocyte primordium   (Fisher et al., 2012) Comment:reported as plasmatocytes anlage ventral epidermis primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval fat body   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) plasmatocyte   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult heart, adult fat body, adult spermathecae, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval trachea, adult ovary, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca    3020.9 Adult InseminatedFemale Spermatheca    2807.3 Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland    1947.3 Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass  528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion  514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body  (997.4) Adult Salivary Gland    40 Adult Heart  (899.775) Adult VirginFemale Spermatheca  (3020.9) Adult InseminatedFemale Spermatheca  (2807.3) Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland  (1947.3) Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca  (3020.9) Adult InseminatedFemale Spermatheca  (2807.3) Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland  (1947.3) Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca    3020.9 Adult InseminatedFemale Spermatheca    2807.3 Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland    1947.3 Adult Carcass    351.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca    3020.9 Adult InseminatedFemale Spermatheca    2807.3 Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland    1947.3 Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass  528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion  514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body  (997.4) Adult Salivary Gland    40 Adult Heart  (899.775) Adult VirginFemale Spermatheca  (3020.9) Adult InseminatedFemale Spermatheca  (2807.3) Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland  (1947.3) Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca  (3020.9) Adult InseminatedFemale Spermatheca  (2807.3) Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland  (1947.3) Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    389.425 Larval Midgut    103.9 Larval Hindgut    135.7 Larval Malpighian Tubules    365.1 Larval Fat Body    388.2 Larval Salivary Gland    42.3 Larval Trachea    104.65 Larval Carcass    528.675 Adult Head    281.2 Adult Eye    254.5 Adult Brain    431 Adult Thoracic-Abdominal Ganglion    514.9 Adult Crop    465.7 Adult Midgut    89.2 Adult Hindgut    384.2 Adult Malpighian Tubules    239.1 Adult Fat Body    997.4 Adult Salivary Gland    40 Adult Heart    899.775 Adult VirginFemale Spermatheca    3020.9 Adult InseminatedFemale Spermatheca    2807.3 Adult Ovary    159.6 Adult Testis    71.3 Adult Male Accessory Gland    1947.3 Adult Carcass    351.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    281.2   NA  Eye    254.5   NA  Brain    431   389.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    514.9   NA  Crop    465.7   103.9  Midgut    89.2   135.7  Hindgut    384.2   365.1  Malpighian Tubules    239.1   388.2  Fat Body    997.4   42.3  Salivary Gland    40   NA  Heart    899.775   104.65  Trachea    NA   NA  VirginFemale Spermatheca    3020.9   NA  InseminatedFemale Spermatheca    2807.3   NA  Ovary    159.6   NA  Testis    71.3   NA  Male Accessory Gland    1947.3   528.675  Carcass    351.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (53) digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae  (53) digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    7 central nervous system, larvae L3    17 central nervous system, pupae P8    23 head, virgin 1-day female    14 head, virgin 4-day female    8 head, virgin 20-day female    8 head, mated 1-day female    9 head, mated 4-day female    9 head, mated 20-day female    7 head, mated 1-day male    12 head, mated 4-day male    13 head, mated 20-day male    13 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    53 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    5 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    9 fat body, white prepupae    11 fat body, pupae P8    6 carcass, larvae L3 wandering    16 carcass, 1-day adult    12 carcass, 4-day adult    10 carcass, 20-day adult    11 ovary, virgin 4-day female    5 ovary, mated 4-day female    5 testis, mated 4-day male    4 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr    122 embryo 12-14hr    108 embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr  (31) embryo 06-08hr  (52) embryo 08-10hr  (63) embryo 10-12hr  (122) embryo 12-14hr  (108) embryo 14-16hr  (71) embryo 16-18hr  (38) embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2  (28) larva L3 12hr old  (38) larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9  (27) white prepupae new  (39) white prepupae 12hr  (39) white prepupae 24hr  (34) pupae 2d postWPP  (37) pupae 3d postWPP  (35) pupae 4d postWPP  (33) adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day  26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr  (122) embryo 12-14hr  (108) embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr    122 embryo 12-14hr    108 embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr    122 embryo 12-14hr    108 embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr  31 embryo 06-08hr  (52) embryo 08-10hr  (63) embryo 10-12hr  (122) embryo 12-14hr  (108) embryo 14-16hr  (71) embryo 16-18hr  (38) embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2  28 larva L3 12hr old  (38) larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9  27 white prepupae new  (39) white prepupae 12hr  (39) white prepupae 24hr  (34) pupae 2d postWPP  (37) pupae 3d postWPP  (35) pupae 4d postWPP  (33) adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day  26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr  122 embryo 12-14hr  108 embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    11 embryo 04-06hr    31 embryo 06-08hr    52 embryo 08-10hr    63 embryo 10-12hr    122 embryo 12-14hr    108 embryo 14-16hr    71 embryo 16-18hr    38 embryo 18-20hr    20 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    18 larva L2    28 larva L3 12hr old    38 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    27 white prepupae new    39 white prepupae 12hr    39 white prepupae 24hr    34 pupae 2d postWPP    37 pupae 3d postWPP    35 pupae 4d postWPP    33 adult male 01day    21 adult male 05day    17 adult male 30day    14 adult female 01day    26 adult female 05day    25 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    385 embryonic 1182-4H    170 embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3    171 leg disc CME L1    306 wing disc CME-W2    82 wing disc ML-DmD8    168 wing disc ML-DmD9    160 wing disc ML-DmD16-c3    123 wing disc ML-DmD21    231 wing disc ML-DmD32    107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11    106 antennal disc ML-DmD20-c5    182 mixed discs ML-DmD4-c1    137 CNS ML-DmBG1-c1    181 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    270 ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (385) embryonic 1182-4H  (170) embryonic GM2  (28) embryonic Kc167  (80) embryonic S1  (34) embryonic S3  (171) leg disc CME L1  (306) wing disc CME-W2  (82) wing disc ML-DmD8  (168) wing disc ML-DmD9  (160) wing disc ML-DmD16-c3  (123) wing disc ML-DmD21  (231) wing disc ML-DmD32  (107) haltere disc ML-DmD17-c3  (80) eye-antennal disc ML-DmD11  (106) antennal disc ML-DmD20-c5  (182) mixed discs ML-DmD4-c1  (137) CNS ML-DmBG1-c1  (181) CNS ML-DmBG2-c2  (70) tumorous blood cells mbn2  (270) ovary fGS/OSS  (27) ovary OSC  (27) ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (385) embryonic 1182-4H  (170) embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3  (171) leg disc CME L1  (306) wing disc CME-W2    82 wing disc ML-DmD8  (168) wing disc ML-DmD9  (160) wing disc ML-DmD16-c3  (123) wing disc ML-DmD21  (231) wing disc ML-DmD32  107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11  106 antennal disc ML-DmD20-c5  (182) mixed discs ML-DmD4-c1  (137) CNS ML-DmBG1-c1  (181) CNS ML-DmBG2-c2    70 tumorous blood cells mbn2  (270) ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    385 embryonic 1182-4H    170 embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3    171 leg disc CME L1    306 wing disc CME-W2    82 wing disc ML-DmD8    168 wing disc ML-DmD9    160 wing disc ML-DmD16-c3    123 wing disc ML-DmD21    231 wing disc ML-DmD32    107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11    106 antennal disc ML-DmD20-c5    182 mixed discs ML-DmD4-c1    137 CNS ML-DmBG1-c1    181 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    270 ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    385 embryonic 1182-4H    170 embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3    171 leg disc CME L1    306 wing disc CME-W2    82 wing disc ML-DmD8    168 wing disc ML-DmD9    160 wing disc ML-DmD16-c3    123 wing disc ML-DmD21    231 wing disc ML-DmD32    107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11    106 antennal disc ML-DmD20-c5    182 mixed discs ML-DmD4-c1    137 CNS ML-DmBG1-c1    181 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    270 ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (385) embryonic 1182-4H  (170) embryonic GM2  28 embryonic Kc167  (80) embryonic S1  (34) embryonic S3  (171) leg disc CME L1  (306) wing disc CME-W2  (82) wing disc ML-DmD8  (168) wing disc ML-DmD9  (160) wing disc ML-DmD16-c3  (123) wing disc ML-DmD21  (231) wing disc ML-DmD32  (107) haltere disc ML-DmD17-c3  (80) eye-antennal disc ML-DmD11  (106) antennal disc ML-DmD20-c5  (182) mixed discs ML-DmD4-c1  (137) CNS ML-DmBG1-c1  (181) CNS ML-DmBG2-c2  (70) tumorous blood cells mbn2  (270) ovary fGS/OSS  27 ovary OSC  27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (182) Schneider line 2 Sg4  (385) embryonic 1182-4H  (170) embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3  (171) leg disc CME L1  (306) wing disc CME-W2    82 wing disc ML-DmD8  (168) wing disc ML-DmD9  (160) wing disc ML-DmD16-c3  123 wing disc ML-DmD21  (231) wing disc ML-DmD32  107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11  106 antennal disc ML-DmD20-c5  (182) mixed discs ML-DmD4-c1  137 CNS ML-DmBG1-c1  (181) CNS ML-DmBG2-c2    70 tumorous blood cells mbn2  (270) ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    182 Schneider line 2 Sg4    385 embryonic 1182-4H    170 embryonic GM2    28 embryonic Kc167    80 embryonic S1    34 embryonic S3    171 leg disc CME L1    306 wing disc CME-W2    82 wing disc ML-DmD8    168 wing disc ML-DmD9    160 wing disc ML-DmD16-c3    123 wing disc ML-DmD21    231 wing disc ML-DmD32    107 haltere disc ML-DmD17-c3    80 eye-antennal disc ML-DmD11    106 antennal disc ML-DmD20-c5    182 mixed discs ML-DmD4-c1    137 CNS ML-DmBG1-c1    181 CNS ML-DmBG2-c2    70 tumorous blood cells mbn2    270 ovary fGS/OSS    27 ovary OSC    27 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    13 Cadmium 50 mM 12 hrs, larvae L3    12 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    8 Paraquat 10 mM 48 hrs, 4-day adult    9 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_25 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • SD05564 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039776 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31022-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for PH4αEFB Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable PH4αEFB02 PH4αEFBEY13295 viable, with Scer\GAL4pnr-MD237 PH4αEFBGD771 Sterility Allele fertile PH4αEFBEY13295 Phenotype manifest in Allele
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of PH4αEFB Class Mutagen Stocks Known lesion PH4αEFB02 1 -- PH4αEFBc00724 1 -- PH4αEFBc02688 piggyBac transposase 1 -- PH4αEFBe02827 piggyBac transposase 1 -- PH4αEFBEY13295 P-element activity 1 -- PH4αEFBMB08046 1 -- PH4αEFBMI02864 1 -- PH4αEFBMI06111 1 -- PH4αEFBc00499 0 -- PH4αEFBCPTI002064 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of PH4αEFB Class Mutagen Stocks Known lesion PH4αEFBGD771 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PH4αEFBHMS00567 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes PH4αEFBHMS00835 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6215 (Ryder, 2004.4.26) Df(3R)Exel6216 (Ryder, 2004.4.26, Abrams et al., 2006)
Partially disrupted in	Df(3R)slab-3 (Rohrbough et al., 2004)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}PH4αEFBMI02864 NA Mi{MIC}PH4αEFBMI06111 NA P{UASp-YFP.Rab14.S49N}PH4αEFB02 NA PBac{681.P.FSVS-1}PH4αEFBCPTI002064 NA PBac{PB}PH4αEFBc00499 NA PBac{PB}PH4αEFBc00724 NA PBac{PB}PH4αEFBc02688 NA PBac{RB}PH4αEFBe02827 NA insertion of mobile activating element P{EPgy2}PH4αEFBEY13295 NA insertion of enhancer trap binary system Mi{ET1}PH4αEFBMB08046 No
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
iron ion binding inferred from electronic annotation with InterPro:IPR006620 (FlyBase Curators et al., 2004-) L-ascorbic acid binding inferred from electronic annotation with InterPro:IPR006620 (FlyBase Curators et al., 2004-) oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen inferred from electronic annotation with InterPro:IPR013547 (FlyBase Curators et al., 2004-) procollagen-proline 4-dioxygenase activity inferred from sequence or structural similarity with mouse P4ha1 (FlyBase, 1992-)
Biological Process
CV term Evidence References
oxidation-reduction process inferred from electronic annotation with InterPro:IPR005123, InterPro:IPR006620, InterPro:IPR013547 (FlyBase Curators et al., 2004-) peptidyl-proline hydroxylation to 4-hydroxy-L-proline inferred from sequence or structural similarity with mouse P4ha1 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
endoplasmic reticulum inferred from electronic annotation with InterPro:IPR013547 (FlyBase Curators et al., 2004-) procollagen-proline 4-dioxygenase complex inferred by curator from GO:0004656|GO:0018401 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
PH4αEFB - Aplip1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Atf-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG10754 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG1092 experimental knowledge based (Guruharsha et al., 2011) PH4αEFB - CG11060 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) PH4αEFB - CG11095 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG11911 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG14232 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG14826 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG2211 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG30090 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG31199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG31445 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG31637 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG32698 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG3726 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG6712 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG8043 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CG9547 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - CycK anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Cyp4d2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Cyp4g15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Fer1HCH experimental knowledge based (Guruharsha et al., 2011) PH4αEFB - Gpb5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - HPS1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Jon44E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Pdi experimental knowledge based (Guruharsha et al., 2011) PH4αEFB - Prosβ2R2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - Rcd6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - dome anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - fau anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - gcl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - gro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - l(2)dtl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - mei-S332 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - stv anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) PH4αEFB - wcd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions PH4αEFB allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68473 DroID - A comprehensive database of gene and protein interactions. • FBgn0039776 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43620
Orthologs
OrthoDB Orthologs (61) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6D53HB Dipteran inclusive ortholog search EOG65X84F Insect inclusive ortholog search EOG65QG8F Arthropod inclusive ortholog search EOG6SXKTQ Metazoa inclusive ortholog search EOG69SK0M
Orthologs in Drosophila Species (EOG6D53HB)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\PH4αEFB     Drosophila simulans   Dsim\GD21521 Y   Drosophila sechellia   Dsec\GM12882 Y   Drosophila erecta   Dere\GG11753 Y   Drosophila yakuba   Dyak\GE10881 Y   Drosophila ananassae   Dana\GF22904 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15946 Y   Drosophila persimilis   Dper\GL13463 Y   Drosophila willistoni   Dwil\GK14136 Y   Drosophila virilis   Dvir\GJ24502 Y   Drosophila mojavensis   Dmoj\GI24860 Y   Drosophila grimshawi   Dgri\GH12976     Drosophila grimshawi   Dgri\GH14110 Y
Orthologs in non-Drosophila Dipterans (EOG65X84F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007038   Anopheles gambiae Malaria mosquito  Agam\AGAP000971   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017131
Orthologs in non-Dipteran Insects (EOG65QG8F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB41280   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010069   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12067   Atta cephalotes Leafcutter ant  Acep\ACEP10116   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20271   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14300   Linepithema humile Argentine ant  Lhum\LH15922   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24528   Solenopsis invicta Red fire ant  Sinv\SINV18462   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007079   Bombyx mori Silkmoth  Bmor\BGIBMGA010566   Pediculus humanus Human body louse  Phum\PHUM445570   Tribolium castaneum Red flour beetle  Tcas\TC011321   Tribolium castaneum Red flour beetle  Tcas\TC011322
Orthologs in non-Insect Arthropods (EOG6SXKTQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_45616   Daphnia pulex Water flea  Dpux\COI   Daphnia pulex Water flea  Dpux\rbcL   Daphnia pulex Water flea  Dpux\COI   Daphnia pulex Water flea  Dpux\JGI_V11_309107   Daphnia pulex Water flea  Dpux\JGI_V11_309117   Ixodes scapularis Deer tick  Isca\ISCW001441
Orthologs in non-Arthropod Metazoa (EOG69SK0M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\dpy-18 Y Caenorhabditis elegans Nematode  Cele\phy-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_027669gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014011gn Y Danio rerio Zebrafish  Drer\si:ch211-146f4.5 Y Danio rerio Zebrafish  Drer\p4ha2 Y Danio rerio Zebrafish  Drer\ENSDARG00000034058 Y Danio rerio Zebrafish  Drer\p4ha1 Y Danio rerio Zebrafish  Drer\si:ch73-68b22.2 Y Danio rerio Zebrafish  Drer\ENSDARG00000033537 Y Xenopus tropicalis Western clawed frog  Xtro\p4ha1 Y Xenopus tropicalis Western clawed frog  Xtro\p4ha2 Y Xenopus tropicalis Western clawed frog  Xtro\p4ha3 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031257 Y Gallus gallus Domestic chicken  Ggal\P4HA1 Y Gallus gallus Domestic chicken  Ggal\P4HA2 Y Gallus gallus Domestic chicken  Ggal\P4HA3 Y Mus musculus House mouse  Mmus\P4ha1 Y Mus musculus House mouse  Mmus\ENSMUSG00000051048 Y Rattus norvegicus Norway rat  Rnor\P4ha2 Y Rattus norvegicus Norway rat  Rnor\P4ha3 Y Homo sapiens Human  Hsap\P4HA3 Y Homo sapiens Human  Hsap\P4HA1 Y Homo sapiens Human  Hsap\P4HA2 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\P4HA3     Hsap\P4HA1     Hsap\P4HA2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21521 Drosophila sechellia Dsec\GM12882 Drosophila erecta Dere\GG11753 Drosophila yakuba Dyak\GE10881 Drosophila ananassae Dana\GF22904 Drosophila pseudoobscura pseudoobscura Dpse\GA15946 Drosophila persimilis Dper\GL13463 Drosophila willistoni Dwil\GK14136 Drosophila virilis Dvir\GJ24502 Drosophila mojavensis Dmoj\GI24860 Drosophila grimshawi Dgri\GH14110
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	26095 w1118; Mi{ET1}PH4αEFBMB08046 33695 y1 sc* v1; P{TRiP.HMS00567}attP2 33896 y1 sc* v1; P{TRiP.HMS00835}attP2 21090 y1 w67c23; P{EPgy2}PH4αEFBEY13295 23264 y1 w*; P{UASp-YFP.Rab14.S49N}PH4αEFB02 36435 y1 w*; Mi{MIC}PH4αEFBMI02864/TM3, Sb1 Ser1 43854 y1 w*; Mi{MIC}PH4αEFBMI06111/TM3, Sb1 Ser1
Harvard	- PBac{PB}PH4αEFBc00724 - PBac{PB}PH4αEFBc02688 - PBac{RB}PH4αEFBe02827
VDRC	v2464 w1118; P{GD771}v2464
Genomic Clones ( 1 )
	BACR48G16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 43 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP05501 LP18565 SD05564
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT25627 GH20626 GH22253 GM01865 GM05509 GM26367 RE22371 RE50230 RE60307 RE63544 RE69137 RE70217 RH36835 RH68472 SD02083 SD02160 SD03106 SD03731 SD03827 SD03833 SD08169 SD11305 SD11345 SD13039 SD13709 SD17813 SD18654 SD19556 SD19561 SD19824 SD22366 SD23727 SD26224 SD26256 SD26411 SD26504
Other clones	7658 EK125557 IP08829 SD03105
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0039776 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43620
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: PH4αEFB CG1473 (Abrams, 2002.3.26, FlyBase, 1996-)
Source for database merge of	Source for merge of: CG1473 CG12088 (FlyBase, 1996-) Source for merge of: CG12088 CG1473 (Abrams, 2002.3.26) Source for merge of: PH4αEFB BcDNA:SD05564 (FlyBase, 1996-)
Additional comments	Annotations CG12088, CG1473 merged as CG31022 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of PH4αEFB BcDNA:SD05564 was a shared cDNA (date:020730). (FlyBase, 1996-) Source for identity of PH4αEFB CG1473 was sequence comparison (date:020425). (FlyBase, 1996-) Source for merge of CG1473 CG12088 was a shared cDNA (date:010720). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170492 RefSeq (Proteins) • NP_733371 DDBJ / EMBL / GenBank • AAF57053 AAK93533 AAM18058 AF495536 AI533665 AY052109 CZ468145 CZ480599 CZ480600 Entrez Gene - A searchable database of RefSeq genes. • 43620 UniProtKB/TrEMBL • Q9VA69
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Oxoglutarate/iron-dependent dioxygenase (IPR005123) Prolyl 4-hydroxylase, alpha subunit (IPR006620) Prolyl 4-hydroxylase alpha-subunit, N-terminal (IPR013547) Tetratricopeptide-like helical (IPR011990) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD05564
Linkouts
	BioGRID - A database of protein and genetic interactions • 68473 DroID - A comprehensive database of gene and protein interactions. • FBgn0039776 DRSC - Results from RNAi screens. • FBgn0039776 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0039776 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31022-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0039776 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43620 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43620 modMine - Data generated by the modENCODE project. • FBgn0039776
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BcDNA:SD05564 (Abrams and Andrew, 2002, ) CG1473 (Abrams, 2002.3.26, ) CG12088 (Abrams, 2002.3.26, ) CG31022 (Ni et al., 2010.12.1) PH4-aEFB (Pastor-Pareja and Xu, 2011) PH4alphaEFB (Giot et al., 2003, Ni et al., 2006, Rohrbough et al., 2004) PH4αEFB (Abrams et al., 2006, White et al., 2011, Zhang et al., 2007, Pastor-Pareja and Xu, 2011, Lerner et al., 2013, Cherbas et al., 2011) PH4α-EFB (Pastor-Pareja and Xu, 2011)
Name Synonym	prolyl-4-hydroxylase-alpha EFB (Abrams and Andrew, 2002) propyl-4-hydroxylase-alpha EFB (Cherbas et al., 2011)
Secondary FlyBase IDs
	FBgn0039775 FBgn0046215 FBgn0051022
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 9 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 14 ) FlyBase analysis  ( 4 ) Website  ( 1 ) Curated genome annotation  ( 3 ) DNA/RNA sequence record  ( 1 )
Recent research papers ( 5 )
	Lerner et al., 2013, Dev. Cell 24(2): 159--168 A Rab10-Dependent Mechanism for Polarized Basement Membrane Secretion during Organ Morphogenesis. [FBrf0220718] Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Pastor-Pareja and Xu, 2011, Dev. Cell 21(2): 245--256 Shaping Cells and Organs in Drosophila by Opposing Roles of Fat Body-Secreted Collagen IV and Perlecan. [FBrf0214668] White et al., 2011, J. Cell Biol. 193(4): 677--694 Drosophila histone locus bodies form by hierarchical recruitment of components. [FBrf0213662] Show all research papers ...