FlyBase Gene Report: Dmel\Jon99Fii

General Information

Symbol

Dmel\Jon99Fii

Species

D. melanogaster

Name

Jonah 99Fii

Annotation Symbol

CG2229

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039777

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Jon99F, SP135

Key Links

Genomic Location

Cytogenetic map

99F6-99F6

Sequence location

3R:30,487,016..30,487,928 [-]

Recombination map

3-100

RefSeq locus

NT_033777 REGION:30487016..30487928

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables serine hydrolase activity

inferred from high throughput direct assay

(Kumar et al., 2021)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables endopeptidase activity

inferred from sequence model

(Ross et al., 2003)

enables serine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Cao and Jiang, 2018)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Ross et al., 2003)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

S1A SERINE PROTEASES - CHYMOTRYPSIN-LIKE

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

S1A SERINE PROTEASES - CHYMOTRYPSIN-LIKE -: Chymotrypsin-like serine proteases cleave substrates following one of the hydrophobic amino acids at the P1 position. (Adapted from FBrf0239469). Chymotrypsin-like serine proteases of the S1A family are endopeptidases that cleave substrates following one of the hydrophobic amino acids at the P1 position. (Adapted from FBrf0239469).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Jon99Fii for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VA67)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Jon99Fii-RA

FBtr0085690

NM_143542

913

267

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Jon99Fii-PA

FBpp0085052

28.9

267

7.04

NP_651799

AAF57055

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VA67

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Jon99Fii using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Jon99Fii_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Jon99Fii in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Jon99Fii

View exonic expression by tissue for Dmel\Jon99Fii

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039777

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43621/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039777

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039777

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2229

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Jon99Fii

Transgenic constructs containing regulatory region of Jon99Fii

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel6215

(Ryder, 2004.4.26)

Df(3R)Exel6216

(Abrams et al., 2006, Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Jon99Fii^GD6349

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (15)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CTRB2

1 of 15

Yes

Hsap\CTRC

1 of 15

Yes

Hsap\CTRL

1 of 15

Yes

Hsap\PROC

1 of 15

Yes

Hsap\PRSS8

1 of 15

Yes

Hsap\PRSS21

1 of 15

Yes

Hsap\PRSS22

1 of 15

Yes

Hsap\PRSS27

1 of 15

Yes

Hsap\PRSS33

1 of 15

Yes

Hsap\PRSS41

1 of 15

Yes

Hsap\PRSS48

1 of 15

Yes

Hsap\ROCK2

1 of 15

Yes

Hsap\TPSAB1

1 of 15

Yes

Hsap\TPSD1

1 of 15

Yes

Hsap\TPSG1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (23)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cela1

2 of 15

Yes

Mmus\Ctrc

2 of 15

Yes

Mmus\Ctrl

2 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Cela3b

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Tpsab1

1 of 15

Mmus\Tpsb2

1 of 15

Mmus\Tpsg1

1 of 15

Rattus norvegicus (Norway rat) (20)

Rnor\Ctrb1

2 of 13

Yes

Rnor\Ctrc

2 of 13

Yes

Rnor\Cela1

1 of 13

Yes

Rnor\Cela2a

1 of 13

Yes

Rnor\Cela3b

1 of 13

Yes

Rnor\Ctrl

1 of 13

Rnor\Habp2

1 of 13

Yes

Rnor\Klk1b3

1 of 13

Rnor\Mcpt2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\cela2a

1 of 12

Yes

Xtro\cela3b

1 of 12

Yes

Xtro\ctrb1

1 of 12

Yes

Xtro\ctrb2

1 of 12

Yes

Xtro\ctrl

1 of 12

Yes

Xtro\LOC100489516

1 of 12

Yes

Xtro\LOC101733231

1 of 12

Yes

Xtro\prss27

1 of 12

Yes

Xtro\prss29

1 of 12

Yes

Danio rerio (Zebrafish) (25)

Drer\cela1.1

1 of 15

Yes

Drer\cela1.2

1 of 15

Yes

Drer\cela1.3

1 of 15

Yes

Drer\cela1.4

1 of 15

Yes

Drer\cela1.6

1 of 15

Yes

Drer\ctrb1

1 of 15

Yes

Drer\ctrl

1 of 15

Yes

Drer\ela2

1 of 15

Yes

Drer\ela2l

1 of 15

Yes

Drer\ela3l

1 of 15

Yes

Drer\f7l

1 of 15

Yes

Drer\LOC100004427

1 of 15

Yes

Drer\prss59.2

1 of 15

Yes

Drer\prss60.3

1 of 15

Yes

Drer\si:dkey-16l2.17

1 of 15

Yes

Drer\si:dkey-78l4.10

1 of 15

Yes

Drer\si:dkey-238d18.3

1 of 15

Yes

Drer\si:dkeyp-93a5.3

1 of 15

Yes

Drer\zgc:112160

1 of 15

Yes

Drer\zgc:112285

1 of 15

Yes

Drer\zgc:136461

1 of 15

Yes

Drer\zgc:154142

1 of 15

Yes

Drer\zgc:163079

1 of 15

Yes

Drer\zgc:165423

1 of 15

Yes

Drer\zgc:171592

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\try-4

2 of 15

Yes

Cele\try-5

2 of 15

Yes

Cele\try-10

2 of 15

Yes

Cele\svh-1

1 of 15

Cele\T22A3.6

1 of 15

Yes

Cele\try-3

1 of 15

Cele\try-9

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (100)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

MP1

2 of 10

psh

2 of 10

scaf

2 of 10

snk

2 of 10

SPE

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68474

DroID - A comprehensive database of gene and protein interactions.

FBgn0039777

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-100

Cytogenetic map

99F6-99F6

Sequence location

3R:30,487,016..30,487,928 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99F6-99F6

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99F-99F

(determined by in situ hybridisation)

(Carlson and Hogness, 1985)

Experimentally Determined Recombination Data

Location

3-100

(FlyBase, 2016)

3-98.4

(Comeron et al., 2012)

3-[101]

(Lindsley and Zimm, 1992)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

80210

y¹ sc^* v¹ sev²¹; P{TOE.GS02567}attP40

50648

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03049}attP2

VDRC

v52351

w¹¹¹⁸; P{GD17783}v52351

v52352

w¹¹¹⁸; P{GD17783}v52352

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (162)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

TA01126

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039777

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039777

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43621

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Jon99Fii CG2229

(Schaeffer, 2004.1.1)

Additional comments

Characterization of LAP genes "Lap-A" and "Lap-D" from the distribution and enzymatic activity of their proteins reveals a striking resemblance to the description of Jonah genes in 99C ("Jon99Co", "Jon99Ci", "Jon99Cii" and "Jon99Ciii") and 99F ("Jon99Fi" and "Jon99Fii") and the pattern of expression of their transcripts. At least one of the Jonah genes in 99C or 99F may correspond to "Lap-A" or "Lap-D".

(Collett and Seymour-Jones, 1992)

Other Comments

Origin and Etymology

Discoverer

Etymology

One of a number of genes isolated from a cDNA library made from third instar salivary glands, but subsequently found not to be expressed in the salivary glands but instead expressed specifically and abundantly in the midgut (which resulted in contamination of the original cDNA library). This surprising discovery prompted the name "Jonah" for this family of genes, after the Biblical prophet who unexpectedly turned up in the gut.

(Carlson and Hogness, 1985)

Identification

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43621

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VA67

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9VA67

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VA67

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039777

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039777

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43621/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039777

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Jon99Fii_1

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039777

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2229

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43621

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2229

MARRVEL_MODEL - MARRVEL (model organism gene)

43621

modMine - A data warehouse for the modENCODE project

FBgn0039777

Linkouts

BioGRID - A database of protein and genetic interactions.

68474

DroID - A comprehensive database of gene and protein interactions.

FBgn0039777

DRSC - Results frm RNAi screens

FBgn0039777

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039777

FlyMine - An integrated database for Drosophila genomics

FBgn0039777

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Name Synonyms

Jonah 99F

(Schaeffer, 2004.1.1)

Jonah 99Fii

Secondary FlyBase IDs

FBgn0001289

Datasets (3)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (3)

Result

Result Type

Title

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (45)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections