FB2022_03, released June 9, 2022

Gene: Dmel\CG12069

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General Information
Symbol
Dmel\CG12069
Species
D. melanogaster
Name
Annotation Symbol
CG12069
Feature Type
protein_coding_gene
FlyBase ID
FBgn0039796
Gene Model Status
Current
Stock Availability
7 publicly available
Enzyme Name (EC)
cAMP-dependent protein kinase (2.7.11.11)
Gene Summary
Contribute a Gene Snapshot for this gene.
All Summaries
Key Links
Genomic Location
Cytogenetic map
100A2-100A2
Sequence location
Recombination map
3-101
RefSeq locus
NT_033777 REGION:30693572..30695498
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR000961, InterPro:IPR002290
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables cAMP-dependent protein kinase activity
inferred from sequence or structural similarity with HGNC:9380
(FlyBase Curators, 2008-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in protein kinase A signaling
inferred from biological aspect of ancestor with PANTHER:PTN000682577
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein phosphorylation
inferred from sequence or structural similarity with HGNC:9380
(FlyBase Curators, 2008-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of cAMP-dependent protein kinase complex
inferred from sequence or structural similarity with HGNC:9380
(FlyBase Curators, 2008-)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000682577
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.11)
Summaries
Gene Group (FlyBase)
PROTEIN KINASE A FAMILY -
The Protein Kinase A (PKA) or cAMP-dependent protein kinases are serine/threonine kinases activated by cAMP. The PKA catalytic subunit is maintained in an inactive state in a heterotetrameric complex consisting of two catalytic (C) subunits bound to two regulatory (R) subunits. Binding of cAMP to the R subunits, releases the C subunits from inhibition. (Adapted from PMID:20027184).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\CG12069 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VA47)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
CG12069-RA
FBtr0085728
NM_143562
1750
356
CG12069-RC
FBtr0335455
NM_170517
1259
356
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
CG12069-PA
FBpp0085090
41.0
356
8.39
Q9VA47
NP_651819
AAF57076
CG12069-PC
FBpp0307433
41.0
356
8.39
Q9VA47
NP_733396
AAN14253
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

356 aa isoforms: CG12069-PA, CG12069-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CG12069 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CG12069 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of CG12069
Transgenic constructs containing regulatory region of CG12069
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Gene disrupted in
Df(3R)Exel7378
(Cingolani et al., 2012, Ryder, 2004.4.26)
Gene not disrupted in
Df(3R)BSC504
(Cingolani et al., 2012)
Df(3R)Exel8194
(Cingolani et al., 2012)
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
Sterility
Allele
male semi-sterile
male sterile
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PRKACA
7 of 15
Yes
No
Hsap\PRKACG
7 of 15
Yes
No
Hsap\PRKACB
6 of 15
No
No
Hsap\PRKX
3 of 15
No
No
Hsap\PRKG1
1 of 15
No
No
0  
Hsap\PRKG2
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Prkaca
7 of 15
Yes
No
Mmus\Prkacb
5 of 15
No
No
Mmus\Prkx
3 of 15
No
No
Mmus\Prkg1
1 of 15
No
No
Mmus\Prkg2
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
Rnor\Prkaca
6 of 13
Yes
No
Rnor\Prkacb
5 of 13
No
No
Rnor\LOC102547029
2 of 13
No
Yes
Rnor\Prkx
2 of 13
No
No
Rnor\Prkg2
1 of 13
No
No
0  
Xenopus tropicalis (Western clawed frog) (4)
Xtro\prkaca
6 of 12
Yes
Yes
Xtro\prkacb
5 of 12
No
No
Xtro\prkx
3 of 12
No
No
Xtro\prkg2
1 of 12
No
No
Danio rerio (Zebrafish) (9)
Drer\prkacab
6 of 15
Yes
No
Drer\prkacba
6 of 15
Yes
No
Drer\prkacaa
5 of 15
No
No
Drer\prkacbb
5 of 15
No
No
Drer\prkx
3 of 15
No
No
Drer\prkg1a
1 of 15
No
No
Drer\prkg1b
1 of 15
No
No
Drer\prkg2
1 of 15
No
No
Drer\si:dkey-121j17.5
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\kin-1
6 of 15
Yes
No
Cele\F47F2.1
3 of 15
No
No
Cele\F47F2.2
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AT3G23310
1 of 9
Yes
No
Atha\AT4G14350
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (5)
Scer\TPK2
7 of 15
Yes
No
Scer\TPK1
6 of 15
No
No
Scer\TPK3
6 of 15
No
No
Scer\BCY1
1 of 15
No
No
Scer\YPK3
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\pka1
4 of 12
Yes
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Pka-C1
6 of 10
Pka-C2
6 of 10
Pka-C3
4 of 10
for
2 of 10
Pkg21D
2 of 10
CG4839
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PRKACA; protein kinase cAMP-activated catalytic subunit alpha
    7 of 15
    601639
    PRKACG; protein kinase cAMP-activated catalytic subunit gamma
    7 of 15
    176893
    PRKACB; protein kinase cAMP-activated catalytic subunit beta
    6 of 15
    176892
      PRKX; protein kinase X-linked
      3 of 15
      300083
          Functional Complementation Data
          Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
          Interactions
          Summary of Physical Interactions
          esyN Network Diagram
          Interactions Browser
          Summary of Genetic Interactions
          esyN Network Diagram
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          Starting gene(s)
          Interaction type
          Interacting gene(s)
          Reference
          External Data
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          Pathways
          Signaling Pathways (FlyBase)
          Metabolic Pathways
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          External Data
          Linkouts
          Genomic Location and Detailed Mapping Data
          Chromosome (arm)
          3R
          Recombination map
          3-101
          Cytogenetic map
          100A2-100A2
          Sequence location
          FlyBase Computed Cytological Location
          Cytogenetic map
          Evidence for location
          100A2-100A2
          Limits computationally determined from genome sequence between P{PZ}ncd05884 and P{lacW}l(3)s2500s2500
          Experimentally Determined Cytological Location
          Cytogenetic map
          Notes
          References
          Experimentally Determined Recombination Data
          Location

          3-101

          (FlyBase, 2016)

          3-98.7

          (Comeron et al., 2012)
          Left of (cM)
          Right of (cM)
          Notes
          Stocks and Reagents
          Stocks (7)
          Genomic Clones (13)
           

          Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

          cDNA Clones (13)
           

          Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

          cDNA clones, fully sequenced
          BDGP DGC clones
          Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Additional comments
          Other Comments

          CG12069 protein is present in mature sperm.

          (Lu, 2014.10.7)

          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

          (Kiger et al., 2003)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 38 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          modMine - A data warehouse for the modENCODE project
          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
          Linkouts
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          Synonyms and Secondary IDs (3)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          References (33)