A Database of Drosophila Genes & Genomes

 

Gene Dmel\RhoGAP100F

General Information
SymbolDmel\RhoGAP100FSpeciesD. melanogaster
NameRhoGAP100FAnnotation symbolCG1976
Feature typeprotein_coding_geneFlyBase IDFBgn0039883
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3RRecombination map
Cytogenetic map 100D2-100D3 Sequence location3R:27,658,600..27,670,981 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
This gene is referred to in FlyBase by the symbol RhoGAP100F (CG1976, FBgn0039883). It has the cytological map location 100D2-100D3. Its sequence location is 3R:27658599..27670981. Its molecular function is described as: GTPase activator activity; protein binding. It is involved in the biological process signal transduction. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
100D2-100D3  
Limits computationally determined from genome sequence between P{EP}pygoEP1076 and P{PZ}ttk02667&P{lacW}ttkj6C7  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\RhoGAP100F for information on other features
detailed view FBtr0085869 FBpp0085228 FBti0052826 FBti0051898 FBti0076326 FBti0065044 FBti0064912 FBti0048679
Comments on Gene Model
DGC clone LD28013 appears problematic: incomplete CDS
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
RhoGAP100F-RA
FBtr0085869
 NM_143645
  9025
  1844
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
RhoGAP100F-PA  
FBpp0085228  
195.5  
1844  
9.82  
  NP_651902
  AAF57207
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
C2 calcium-dependent membrane targeting (IPR000008)
RhoGAP (IPR000198)
PDZ/DHR/GLGF (IPR001478)
Rho GTPase activation protein (IPR008936)
C2 calcium/lipid-binding region, CaLB (IPR008973)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA949204
 
AE003780
AAF57207
 
AI532780
 
AW942789
 
AY095038
AAM11366
 
BI640831
 
CK659766
 
CO189883
 
CO275953
 
CO277502
 
CO277570
 
CO286532
 
CO293123
 
CO302760
 
CO312481
 
CO313067
 
CO322158
 
CO323690
 
CZ467414
 
CZ487183
 
CZ487184
 
CZ488877
 
CZ488878
 
EC060305
 
EC071772
 
EC074500
 
EC077768
 
EC077779
 
EC085284
 
EC087708
 
EC087979
 
EC201206
 
EC214732
 
EC218121
 
EC223353
 
EC229215
 
EC243389
 
EC246379
 
EC255581
 
EW712844
 
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (BDGP Project Members, 2000-)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0085869
        AA949204
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Phenotype manifest in
        Allele
        hide Classical Alleles ( 6 )
        For All Classical Alleles Show

        Allele of RhoGAP100FClassMutagenStocksKnown lesion
        RhoGAP100FMB90151
          		1 --
          RhoGAP100Fc011282 --
          RhoGAP100Fc017540 --
          RhoGAP100FdsRNA.H.Scer\UAS
            		0 --
            RhoGAP100Ff046411 --
            RhoGAP100Ff062171 --
            hide Alleles Carried on Transgenic Constructs ( 2 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of RhoGAP100FClassMutagenStocksKnown lesion
            RhoGAP100FGD96940 Yes
            RhoGAP100FdsRNA.Scer\UAS2 Yes
            hide Aneuploid Aberrations
            Useful deficiency
             
            Useful duplication
             
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            UAS construct
            P{GD9694}
            NA
            P{UAS-RhoGAP100F-dsRNA}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            miscellaneous insertions
            PBac{PB}RhoGAP100Fc01128
            NA
            PBac{PB}RhoGAP100Fc01754
            NA
            PBac{WH}RhoGAP100Ff04641
            NA
            PBac{WH}RhoGAP100Ff06217
            NA
            insertion of enhancer trap binary system
            Mi{ET1}RhoGAP100FMB90151
            No
            insertion of enhancer trap
            P{PZ}G45
            No
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            hide Gene Ontology: Function, Process & Cellular Component ( 5 )
            hide Molecular Function
            CV term
            Evidence
            References
            GTPase activator activity
            inferred from sequence or structural similarity with MGD:Sh3bp1; MGI:MGI:104603
            (FlyBase, 1992-)
            protein binding
            inferred from electronic annotation with InterPro:IPR001478
            (FlyBase Curators et al., 2004-)
            hide Biological Process
            CV term
            Evidence
            References
            signal transduction
            inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936
            (FlyBase Curators et al., 2004-)
            hide Cellular Component
            CV term
            Evidence
            References
            cellular_component
            no biological data available
            (FlyBase Curators, 2002-)
            intracellular
            inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936
            (FlyBase Curators et al., 2004-)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            RhoGAP100F allele
            Gene
            References
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 7 )
            Harvard
            -
            PBac{PB}RhoGAP100Fc01128
            -
            PBac{WH}RhoGAP100Ff04641
            -
            PBac{WH}RhoGAP100Ff06217
            Bloomington
            6446
            y1 w*; P{UAS-RhoGAP100F-dsRNA}6.2
            6447
            y1 w*; P{UAS-RhoGAP100F-dsRNA}3.1
            23092
            y1 w67c23; Mi{ET1}RhoGAP100FMB90151
            10374
            w1118; PBac{PB}RhoGAP100Fc01128/TM6B, Tb1
            hide Genomic Clones ( 1 )
            hide cDNA Clones ( 14 )
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for database merge of
            Additional comments
            hide Comments About Role
            hide Comments About Molecular Function
            hide Other Comments
            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
            (Kiger et al., 2003)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •
            UniProtKB/TrEMBL
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            C2 calcium-dependent membrane targeting (IPR000008)
            RhoGAP (IPR000198)
            PDZ/DHR/GLGF (IPR001478)
            Rho GTPase activation protein (IPR008936)
            C2 calcium/lipid-binding region, CaLB (IPR008973)
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DEDB - Drosophila exon database: splicing graphs
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            •
            FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
            •
            GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            InParanoid orthologs - Eukaryotic orthologs
            •
            PANTHER - Protein classification by function, families, and pathways
            •
            University of Chicago Dev. Expression
            •
            hide Synonyms & Secondary IDs ( 3 )
            Reported As
            Symbol Synonym
            1976
            (Hu et al., 2005)
            CG1976
            (Hu, 2005, Bernards, 2003, Billuart et al., 2001, )
            RhoGAP100F
             
            Name Synonym
            RhoGAP100F
             
            Secondary FlyBase IDs
              hide References ( 16 )
              Research paper
              Hu et al., 2005, Proc. Natl. Acad. Sci. USA 102(12): 4613--4618
              Cross GTPase-activating protein (CrossGAP)/Vilse links the Roundabout receptor to Rac to regulate midline repulsion. [FBrf0191476]
              Billuart et al., 2001, Cell 107(2): 195--207
              Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610]
              Supplementary material
              Dietzl et al., 2007, Nature 448(7150):
              Supplementary Table 1. [FBrf0202579]
              Hu, 2005, Proc. Natl. Acad. Sci. USA 102(12):
              [title not yet available] [FBrf0191477]
              Kiger et al., 2003, J. Biol. 2(4):
              Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]
              Review
              Bernards, 2003, Biochim. biophys. Acta 1603(2): 47--82
              GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. [FBrf0158741]
              Personal communication to FlyBase
              Bellen, 2006.8.3, MBBatch686.xml
              MBBatch686.xml [FBrf0191806]
              Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase.
              Exelixis insertion alleles that are not in FlyBase. [FBrf0179797]
              FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.
              Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
              FlyBase analysis
              FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase.
              Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]
              FlyBase, 1992-, FlyBase curation
              FlyBase curation. [FBrf0105495]
              Computer file
              Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.
              Genomic mapping of Exelixis insertion collection. [FBrf0184340]
              Gene Disruption Project members, 2001-, [title not yet available]
              [title not yet available] [FBrf0132177]
              BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.
              Berkeley Drosophila Genome Project. [FBrf0125078]
              DNA/RNA sequence record
              Finkielsztein, 2001.10.19, GenBank/EMBL/DDBJ: AF436843
              Drosophila melanogaster genomic sequence flanking the 5' end of transposon P{PZ}G45 enhancer trap insertion. [FBrf0139920]
              Automatic genome annotation
              FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]
              [Annotations performed at the Celera Jamboreee.] [FBrf0126705]