Gene Dmel\RhoGAP102A

Help

Close All Open All

General Information

Symbol

Dmel\RhoGAP102A

Species

D. melanogaster

Name

RhoGAP102A

Annotation symbol

CG1748

Feature type

protein_coding_gene

FlyBase ID

FBgn0039898

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

102B3-102B3

Sequence location

4:281,943..282,764 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol RhoGAP102A (CG1748, FBgn0039898). It has the cytological map location 102B3. Its sequence location is 4:281943..282764. Its molecular function is described as Rho GTPase activator activity. It is involved in the biological process signal transduction. 2 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102B3-102B3

Limits computationally determined from genome sequence between P{SUPor-P}Crk^KG00336&P{SUPor-P}KG00711 and P{SUPor-P}KG02769

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\RhoGAP102A for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

RhoGAP102A-RA

FBtr0089120

NM_143657

708

202

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

RhoGAP102A-PA

FBpp0088189

23.5

202

8.48

NP_651914

AAF59356

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0039898

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Rho GTPase activation protein (IPR008936)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V4C2

Maps to

Does NOT map to

Identified with

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13620

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0039898

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0039898

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0039898

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of RhoGAP102A	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of RhoGAP102A	Class	Mutagen	Stocks	Known lesion
RhoGAP102A^GD9389		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
RhoGAP102A^{dsRNA.Scer\UAS}		in vitro construct \| regulatory fusion in vitro construct \| RNAi	2	Yes

Aneuploid Aberrations

Not disrupted in

Df(4)B2-2d

(Sousa-Neves et al., 2005)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9389}

P{UAS-RhoGAP102A-dsRNA}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 3 )

Molecular Function

CV term

Evidence

References

Rho GTPase activator activity

inferred from sequence or structural similarity with EMBL:AF022212

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

signal transduction

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

RhoGAP102A allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68616

Orthologs

Genome-wide drosophilid orthologs

Dpse\GA14524

Dere\GG16384

Dgri\GH23982

Dmoj\GI14109

Dper\GL18165

Dsec\GM26791

Dvir\GJ17092

Dwil\GK13678

Dyak\GE14545

Curated drosophilid orthologs

Dpse\GA14524

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

SPBC3F6.05
Schizosaccharomyces pombe (Sanger)
YDR379W
Saccharomyces cerevisiae (Yeastgenome)
MGI:1196332
Mus musculus (Informatics)
DDB0189127
Dictyostelium discoideum (Dictybase)
WBGene00015303
Caenorhabditis elegans (Wormbase)
WBGene00026702
Caenorhabditis briggsae (Wormbase)
AT5G61530.1
Arabidopsis thaliana (Arabidopsis)
ENSXETP00000011716
Xenopus tropicalis (Ensembl)
GSTENP00027202001
Tetraodon nigroviridis (Ensembl)
ENSRNOP00000050852
Rattus norvegicus (Ensembl)
ENSPTRP00000044798
Pan troglodytes (Ensembl)
ENSMODP00000021683
Monodelphis domestica (Ensembl)
ENSMMUP00000005194
Macaca mulatta (Ensembl)
ENSP00000338967
Homo sapiens (Ensembl)
ENSGALP00000026744
Gallus gallus (Ensembl)
NEWSINFRUP00000145933
Takifugu rubripes (Ensembl)
ENSCAFP00000017044
Canis familiaris (Ensembl)
ENSBTAP00000003400
Bos taurus (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

VDRC

v32936

w¹¹¹⁸ P{GD9389}v32936

v32933

w¹¹¹⁸; P{GD9389}v32933

Bloomington

6448

y¹ w^*; P{UAS-RhoGAP102A-dsRNA}3.1

6449

y¹ w^*; P{UAS-RhoGAP102A-dsRNA}7.2

Genomic Clones ( 0 )

cDNA Clones ( 3 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0039898

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0039898

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_143657

RefSeq (Proteins)

•

NP_651914

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q9V4C2

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Rho GTPase activation protein (IPR008936)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68616

DEDB - Drosophila exon database: splicing graphs

•

13620

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0039898

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0039898

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0039898

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0039898

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0039898

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0039898

InParanoid orthologs - Eukaryotic orthologs

•

SPBC3F6.05
Schizosaccharomyces pombe (Sanger)
YDR379W
Saccharomyces cerevisiae (Yeastgenome)
MGI:1196332
Mus musculus (Informatics)
DDB0189127
Dictyostelium discoideum (Dictybase)
WBGene00015303
Caenorhabditis elegans (Wormbase)
WBGene00026702
Caenorhabditis briggsae (Wormbase)
AT5G61530.1
Arabidopsis thaliana (Arabidopsis)
ENSXETP00000011716
Xenopus tropicalis (Ensembl)
GSTENP00027202001
Tetraodon nigroviridis (Ensembl)
ENSRNOP00000050852
Rattus norvegicus (Ensembl)
ENSPTRP00000044798
Pan troglodytes (Ensembl)
ENSMODP00000021683
Monodelphis domestica (Ensembl)
ENSMMUP00000005194
Macaca mulatta (Ensembl)
ENSP00000338967
Homo sapiens (Ensembl)
ENSGALP00000026744
Gallus gallus (Ensembl)
NEWSINFRUP00000145933
Takifugu rubripes (Ensembl)
ENSCAFP00000017044
Canis familiaris (Ensembl)
ENSBTAP00000003400
Bos taurus (Ensembl)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0039898

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

CG1748

(Bernards, 2003, Billuart et al., 2001, )

RhoGAP102A

(Podemski et al., 2004)

Name Synonym

RhoGAP102A

Secondary FlyBase IDs

References ( 10 )

Research paper

Sousa-Neves et al., 2005, Genetics 170(1): 127--138: High-resolution mapping of the Drosophila fourth chromosome using site-directed terminal deficiencies. [FBrf0187662]
Podemski et al., 2004, Chromosoma 112(8): 381--388: Molecular mapping of deletion breakpoints on chromosome 4 of Drosophila melanogaster. [FBrf0179388]
Billuart et al., 2001, Cell 107(2): 195--207: Regulating axon branch stability. The role of p190 RhoGAP in repressing a retraction signaling pathway. [FBrf0139610]

Supplementary material

Kiger et al., 2003, J. Biol. 2(4):: Additional data file 1: The gene identity and primer sequences for dsRNAs used in the RNAi screens. [FBrf0174231]

Review

Bernards, 2003, Biochim. biophys. Acta 1603(2): 47--82: GAPs galore! A survey of putative Ras superfamily GTPase activating proteins in man and Drosophila. [FBrf0158741]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]

FlyBase analysis

FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]

Curated genome annotation

Li, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126682]