FlyBase Gene Report: Dmel\rept

General Information

Symbol

Dmel\rept

Species

D. melanogaster

Name

reptin

Annotation Symbol

CG9750

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040075

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Acts as a transcriptional coactivator in Wg signaling caused by altered arm signaling. Pont and rept interfere antagonistically with nuclear arm signaling function, and are required to enhance or reduce arm activity, respectively. Also an essential cofactor for the normal function of Myc; required for cellular proliferation and growth. Proposed core component of the chromatin remodeling Ino80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. (UniProt, Q9V3K3)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

l(3)06945, dReptin, psg10, Tip48/Reptin, Tip48

Key Links

Genomic Location

Cytogenetic map

76A3-76A3

Sequence location

3L:19,255,340..19,257,111 [+]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:19255340..19257111

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (24 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VH07

(Bellosta et al., 2005)

inferred from physical interaction with UniProtKB:Q9W4S7

(Bellosta et al., 2005)

enables transcription corepressor activity

inferred from mutant phenotype

(Bellosta et al., 2005, Bauer et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR010339

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003593

(InterPro Project Members, 2004-)

enables ATP-dependent activity, acting on DNA

inferred from electronic annotation with InterPro:IPR027238

(InterPro Project Members, 2004-)

enables DNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

Biological Process (12 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in autophagic cell death

inferred from mutant phenotype

(Ihry and Bashirullah, 2014)

involved_in DNA repair-dependent chromatin remodeling

inferred from direct assay

(Kusch et al., 2004)

involved_in heterochromatin formation

inferred from mutant phenotype

(Qi et al., 2006)

involved_in imaginal disc-derived leg morphogenesis

inferred from mutant phenotype

(Ihry and Bashirullah, 2014)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Bauer et al., 2000)

involved_in negative regulation of gene expression

inferred from mutant phenotype

(Diop et al., 2008)

involved_in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I

inferred from mutant phenotype

(Vinayagam et al., 2016)

involved_in regulation of cell population proliferation

inferred from mutant phenotype

(Bellosta et al., 2005)

involved_in response to ecdysone

inferred from mutant phenotype

(Ihry and Bashirullah, 2014)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in box C/D snoRNP assembly

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

involved_in chromatin remodeling

inferred by curator from GO:0031011

(Klymenko et al., 2006)

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from physical interaction with FLYBASE:pont; FB:FBgn0040078

(Kusch et al., 2004)

part_of Ino80 complex

inferred from direct assay

(Klymenko et al., 2006)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Kusch et al., 2004)

inferred from direct assay

(Bauer et al., 2000)

part_of R2TP complex

inferred from direct assay

(Lennon et al., 2022)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

part_of Ino80 complex

inferred from biological aspect of ancestor with PANTHER:PTN000120245

(GO Reference Genome Project, 2011-)

part_of NuA4 histone acetyltransferase complex

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

part_of R2TP complex

inferred from biological aspect of ancestor with PANTHER:PTN000120244

(GO Reference Genome Project, 2011-)

part_of Swr1 complex

inferred from biological aspect of ancestor with PANTHER:PTN000120245

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

INO80 COMPLEX

TIP60 COMPLEX

RUVB-LIKE DNA HELICASES

SWR1 COMPLEX

Protein Family (UniProt)

Belongs to the RuvB family. (Q9V3K3)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Group (FlyBase)

INO80 COMPLEX -: The INO80 complex is an ATP-dependent chromatin remodeller that can mobilize nucleosomes. (Adapted from PMID:17316710).
TIP60 COMPLEX -: The Tip60 chromatin remodeling complex acetylates nucleosomal phospho-histone H2Av and exchanges it with unmodified H2Av. (Adapted from FBrf0185742).
RUVB-LIKE DNA HELICASES -: RuvB-like DNA helicases are homologs of E. coli RuvB, comprising RVB1 and RVB2 (also known as Pontin/Reptin) in eukaryotes. They are two are two highly conserved members of the AAA+ family of ATPases. RuvB-like DNA helicases are part of various chromatin remodeling complexes where they regulate the accessibility of DNA to the proteins involved in transcription and DNA damage repair by regulating the position or modification of nucleosomes. (Adapted from PMID:19524533.)
SWR1 COMPLEX -: In D.mel, the SWR1 complex is an ATP-dependent histone exchanger that incorporates the histone H2A variant, His2Av. (Adapted from FBrf0245697.)

Protein Function (UniProtKB)

(UniProt, Q9V3K3)

Summary (Interactive Fly)

member of AAA+ family of ATPases - DNA helicase - participates in epigenetic processes leading to a repressive chromatin state as part of the TIP60 HAT complex

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\rept for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3K3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

rept-RA

FBtr0074988

NM_079432

1772

481

rept-RB

FBtr0331852

NM_001275111

1707

481

rept-RC

FBtr0344993

NM_001300184

1714

481

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

rept-PA

FBpp0074756

53.5

481

5.61

FBpp0304236

53.5

481

5.61

FBpp0311247

53.5

481

5.61

Q9V3K3

NP_001287113

AHN58138

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

481 aa isoforms: rept-PA, rept-PB, rept-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Forms homohexameric rings. May form a dodecamer with rept made of two stacked hexameric rings (By similarity). Component of the chromatin remodeling Ino80 complex. Interacts with Myc and pont.

(UniProt, Q9V3K3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rept using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rept_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

3.62

Transcript Expression

dissected tissue

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval salivary gland

Comment: from -12 hr APF

(Ihry and Bashirullah, 2014)

prepupal stage P1 -- P4

embryonic/larval salivary gland

Comment: peak at P4

(Ihry and Bashirullah, 2014)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally contributed

(Bauer et al., 2000)

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | intense | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

mesoderm anlage

(Bauer et al., 2000)

endoderm anlage

(Bauer et al., 2000)

embryonic stage 6 -- 8

cephalic furrow

(Bauer et al., 2000)

mesoderm

(Bauer et al., 2000)

posterior endoderm

(Bauer et al., 2000)

anterior endoderm

(Bauer et al., 2000)

embryonic stage 7 -- 8

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 9 -- 15

endodermal derivative

(Bauer et al., 2000)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

lymph gland primordium

Comment: reported as lymph gland specific anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 15 -- 17

chordotonal organ precursor cell

(Bauer et al., 2000)

Additional Descriptive Data

Expression increases after the prepupal (+10 hr APF) ecdysone pulse.

(Ihry and Bashirullah, 2014)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 14 -- 17

endodermal derivative

• nucleus

(Bauer et al., 2000)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Bauer et al., 2000)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of histone acetyltransferase complex

inferred from physical interaction with FLYBASE:pont; FB:FBgn0040078

(Kusch et al., 2004)

part_of Ino80 complex

inferred from direct assay

(Klymenko et al., 2006)

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

located_in nucleus

inferred from direct assay

(Kusch et al., 2004)

inferred from direct assay

(Bauer et al., 2000)

part_of R2TP complex

inferred from direct assay

(Lennon et al., 2022)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\rept in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\rept

View exonic expression by tissue for Dmel\rept

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040075

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40092/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040075

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040075

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040075

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9750

Flygut - An atlas of the Drosophila adult midgut

FBgn0040075

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\rept

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of rept

Transgenic constructs containing regulatory region of rept

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene not disrupted in

Df(3L)VW3

(BDGP Project Members, 1994-1999)

Gene disrupted in

Df(3L)ED229

(Sapiro et al., 2013)

Df(3L)ED4789

(Ihry and Bashirullah, 2014)

Df(3L)fz2

(Ihry and Bashirullah, 2014)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

rept^psg10

lethal, with Scer\GAL4^Act5C.PI

rept^KK105732

lethal, with Scer\GAL4^en.PU

rept^KK105732

lethal, with Scer\GAL4^ey.PH

rept^KK105732

lethal, with Scer\GAL4^insc-Mz1407

rept^GD4561

lethal, with Scer\GAL4^sca.PU

rept^KK105732

lethal | recessive

lethal - all die before end of larval stage

rept³⁵

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

rept^GD4561

lethal - all die before end of larval stage | recessive

rept³⁵

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

rept^GD4561

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

rept^GD4561

partially lethal - majority die, with Scer\GAL4^nub.PK

rept^KK105732

viable

rept^ex1

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

rept^GD4561

viable, with Scer\GAL4^tin.CΔ4

rept^GD4561

Sterility

Allele

male sterile, with Scer\GAL4^VP16.bam

rept^KK105732

Other Phenotypes

Allele

abnormal cytokinesis | spermatogenesis, with Scer\GAL4^VP16.bam

rept^GD4561

abnormal developmental rate | cell autonomous

rept³⁵

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

rept^GD4561

decreased cell number | male, with Scer\GAL4^VP16.bam

rept^KK105732

decreased cell number | somatic clone | third instar larval stage

decreased size | adult stage, with Scer\GAL4^ey.PU

rept^GD4561

non-suppressor of variegation, with P{hsp26-pt-T}39C-5

rept⁰⁶⁹⁴⁵

non-suppressor of variegation, with P{hsp26-pt-T}39C-12

rept⁰⁶⁹⁴⁵

non-suppressor of variegation, with P{hsp26-pt-T}39C-27

rept⁰⁶⁹⁴⁵

some die during larval stage

rept³⁵

some die during larval stage | recessive

rept³⁵

some die during pupal stage, with Scer\GAL4^pnr-MD237

rept^GD4561

suppressor of variegation, with P{hsp26-pt-T}39C-4

rept⁰⁶⁹⁴⁵

suppressor of variegation, with P{hsp26-pt-T}39C-72

rept⁰⁶⁹⁴⁵

suppressor of variegation, with P{hsp26-pt-T}118E-10

rept⁰⁶⁹⁴⁵

suppressor of variegation, with P{hsp26-pt-T}118E-12

rept⁰⁶⁹⁴⁵

suppressor of variegation | dominant

rept^unspecified

suppressor of variegation | dominant, with In(1)w^m4h

rept⁰⁶⁹⁴⁵

suppressor of variegation | dominant, with T(1;4)w^m258-21

rept⁰⁶⁹⁴⁵

visible | adult stage, with Scer\GAL4^ey.PU

rept^GD4561

visible | adult stage, with Scer\GAL4^nub.PK

rept^KK105732

Phenotype manifest in

Allele

adult head, with Scer\GAL4^ey.PU

rept^GD4561

chromatin | spermatogenesis, with Scer\GAL4^VP16.bam

rept^GD4561

embryonic/larval midgut | third instar larval stage

rept^psg10

embryonic/larval salivary gland | prepupal stage

rept^psg10

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^Tub.PU

rept^GD4561

eye, with Scer\GAL4^ey.PU

rept^GD4561

eye, with Scer\GAL4^Tub.PU

rept^UAS.Tag:AU1

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

rept^GD4561

larval brain | somatic clone | third instar larval stage

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

rept^GD4561

leg | prepupal stage

rept^psg10

meiotic spindle | spermatogenesis, with Scer\GAL4^VP16.bam

rept^GD4561

meiotic spindle pole | increased number | spermatogenesis, with Scer\GAL4^VP16.bam

rept^GD4561

ommatidium, with Scer\GAL4^Tub.PU

rept^UAS.Tag:AU1

polytene chromosome | third instar larval stage, with Scer\GAL4^Tub.PU

rept^GD4561

spermatid, with Scer\GAL4^VP16.bam

rept^KK105732

spermatid axoneme, with Scer\GAL4^VP16.bam

rept^KK105732

spermatozoon, with Scer\GAL4^VP16.bam

rept^KK105732

wing, with Scer\GAL4^nub.PK

rept^KK105732

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\RUVBL2

14 of 14

Yes

3 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Ruvbl2

14 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (4)

Mmus\Ruvbl2

14 of 14

Yes

Mmus\Ruvbl1

2 of 14

Mmus\Spata5

1 of 14

Mmus\Yme1l1

1 of 14

Xenopus tropicalis (Western clawed frog) (2)

Xtro\ruvbl2

8 of 13

Yes

Xtro\ruvbl1

2 of 13

Danio rerio (Zebrafish) (4)

Drer\ruvbl2

14 of 14

Yes

Drer\ruvbl1

3 of 14

Drer\yme1l1a

1 of 14

Drer\yme1l1b

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\ruvb-2

14 of 14

Yes

Cele\ruvb-1

3 of 14

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP009746

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\AT3G49830

13 of 13

Yes

Atha\AT5G67630

13 of 13

Yes

Atha\RIN1

3 of 13

Atha\AT1G05910

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\RVB2

13 of 13

Yes

Scer\RVB1

2 of 13

Scer\YTA7

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\rvb2

12 of 12

Yes

Spom\rvb1

2 of 12

Spom\cdc48

1 of 12

Spom\SPAC31G5.19

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:rept. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

pont

5 of 13

smid

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RUVBL2; RuvB like AAA ATPase 2

14 of 14

604788

RUVBL1; RuvB like AAA ATPase 1

3 of 14

603449

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

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Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

rept - CG12911

experimental knowledge based

(Guruharsha et al., 2011)

rept - CG13901

experimental knowledge based

(Guruharsha et al., 2011)

rept - CG5973

experimental knowledge based

(Guruharsha et al., 2011)

rept - CG8745

experimental knowledge based

(Guruharsha et al., 2011)

rept - Dnaaf4

anti tag coimmunoprecipitation, anti tag western blot

(Lennon et al., 2022)

rept - Dnaaf6

anti tag coimmunoprecipitation, anti tag western blot

(Lennon et al., 2022)

rept - Myc

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation

(Bellosta et al., 2005)

rept - Pih1D1

anti tag coimmunoprecipitation, western blot

(Benbahouche et al., 2014)

rept - S6kII

anti tag coimmunoprecipitation, anti tag western blot

(Vinayagam et al., 2016)

rept - Sfmbt

anti tag coimmunoprecipitation, peptide massfingerprinting

(Alfieri et al., 2013)

rept - Tip60

anti bait coimmunoprecipitation, anti tag western blot, anti tag coimmunoprecipitation

(Qi et al., 2006, Kusch et al., 2004)

rept - Tti1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

rept - Wdr92

anti tag coimmunoprecipitation, anti tag western blot

(Zur Lage et al., 2018)

rept - mTor

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

rept - ph-p

anti tag coimmunoprecipitation, peptide massfingerprinting

(Saurin et al., 2001)

rept - pho

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Klymenko et al., 2006)

rept - pont

experimental knowledge based, anti tag coimmunoprecipitation, western blot, peptide massfingerprinting

(Guruharsha et al., 2011, Bellosta et al., 2005, Kusch et al., 2004)

rept - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rept - spag

anti tag coimmunoprecipitation, peptide massfingerprinting

(Benbahouche et al., 2014)

rept - wcy

anti tag coimmunoprecipitation, peptide massfingerprinting

(David-Morrison et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

rept

enhanceable

CG8677

(Hanai et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

arm

enhanceable

rept

(Bauer et al., 2000)

brm

suppressible

rept

(Diop et al., 2008)

CG8677

enhanceable

rept

(Hanai et al., 2008)

dom

enhanceable

rept

(Kwon et al., 2013)

suppressible

rept

(Carrera et al., 1998)

Myc

enhanceable

rept

(Bellosta et al., 2005)

enhanceable

(Qi et al., 2006)

suppressible

(Wilk et al., 2004)

enhanceable

(Qi et al., 2006)

suppressible

(Bauer et al., 2000)

External Data

Subunit Structure (UniProtKB)

Forms homohexameric rings. May form a dodecamer with rept made of two stacked hexameric rings (By similarity). Component of the chromatin remodeling Ino80 complex. Interacts with Myc and pont.

(UniProt, Q9V3K3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65368

DroID - A comprehensive database of gene and protein interactions.

FBgn0040075

MIST (protein-protein) - An integrated Molecular Interaction Database

40092

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

ATP-dependent chromatin remodeling

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

76A3-76A3

Sequence location

3L:19,255,340..19,257,111 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76A3-76A3

Limits computationally determined from genome sequence between P{EP}MESR6^EP3142 and P{lacW}l(3)L3809^L3809

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76A3-76A4

(determined by in situ hybridisation)

(Spradling et al., 1999)

76A3-76A4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-55

(Sapiro et al., 2013)

3-42.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18476

w¹¹¹⁸; PBac{WH}rept^f01801/TM6B, Tb¹

11706

P{PZ}rept⁰⁶⁹⁴⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

21384

y¹ w^67c23; P{EPgy2}rept^EY12756/TM3, Sb¹ Ser¹

32415

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00410}attP2/TM3, Sb¹

64755

P{ey-FLP.N}2, w^* P{P-Sal}Myc^P0 P{αTub84B(FRT.Myc)GAL4.Bb}1/FM7a; rept³⁵/MKRS

FlyORF

F001385

M{UAS-rept.ORF}ZH-86Fb

NIG-Fly

9750R-1

P{NIG.9750R}1

9750R-2

P{NIG.9750R}2

HMS00410

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00410}attP2/TM3, Sb¹

VDRC

v103483

P{KK105732}VIE-260B

v19021

w¹¹¹⁸; P{GD4561}v19021

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (75)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD12420

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040075

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040075

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Diop et al., 2008, Ducat et al., 2008, Bauer et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: reptin CG9750

(Bayraktaroglu, 2000.8.7)

Source for merge of: l(3)06945 reptin

(Bauer et al., 2000)

Source for merge of: rept anon-WO0142479.1

(FlyBase, 1996-)

Additional comments

The rept gene may have been derived from the pont gene by retroposition.

(Pan and Zhang, 2009)

Source for merge of rept anon-WO0142479.1 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: rept reptin

(Pradel, 2001.1.25)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG9750

(Zur Lage et al., 2018, Kolkhof et al., 2017, Hosono et al., 2015, Benbahouche et al., 2014, Schertel et al., 2013, Glatter et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Hughes et al., 2008, Kusch et al., 2004)

Q9V3K3_DROME

(Fukuyama et al., 2012)

REP

(Bouazoune and Brehm, 2006)

REPT

(Fukuyama et al., 2013, Kondo and Perrimon, 2011)

Rept

(Rust et al., 2018, David-Morrison et al., 2016, Klymenko et al., 2006)

Reptin

(Clapier and Cairns, 2009, Stadeli, 2006)

Tip48

(Stadeli, 2006, Hulf and Gallant, 2003)

Tip48/Reptin

(Bellosta et al., 2005, Gallant et al., 2003)

anon-WO0142479.1

dRUVBL2

(Yasunaga et al., 2014)

dRep

(Kusch et al., 2004)

dReptin

(Hanai et al., 2008, Kusch et al., 2004, Kusch et al., 2004)

drep

(Bauer et al., 2000)

l(3)06945

(Spradling et al., 1999, Carrera et al., 1998, BDGP Project Members, 1994-1999)

psg10

(Ihry and Bashirullah, 2014, Sapiro et al., 2013)

rep

(Bauer et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBgn0010908
FBgn0036854
FBgn0044525

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40092

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3K3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3K3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PFN1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3K3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040075

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40092/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040075

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040075

FlyMine - An integrated database for Drosophila genomics

FBgn0040075

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9750

MARRVEL_MODEL - MARRVEL (model organism gene)

40092

Linkouts

BioGRID - A database of protein and genetic interactions.

65368

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040075

DroID - A comprehensive database of gene and protein interactions.

FBgn0040075

DRSC - Results frm RNAi screens

FBgn0040075

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rept_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040075

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040075

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9750

Flygut - An atlas of the Drosophila adult midgut

FBgn0040075

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015193

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

ATP-dependent chromatin remodeling

MIST (protein-protein) - An integrated Molecular Interaction Database

40092

References (127)

The following external sites may use different assemblies or annotations than FlyBase.

3.62

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