Open Close
General Information
Symbol
Dmel\pins
Species
D. melanogaster
Name
partner of inscuteable
Annotation Symbol
CG5692
Feature Type
FlyBase ID
FBgn0040080
Gene Model Status
Stock Availability
Gene Snapshot
partner of inscuteable (pins) encodes a GDP-dissociation inhibitor involved in asymmetric cell division and mitotic spindle orientation. [Date last reviewed: 2019-06-13]
Also Known As

raps, Rapsynoid, Rad

Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:27,582,840..27,586,376 [+]
Recombination map

3-94

RefSeq locus
NT_033777 REGION:27582840..27586376
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
inferred from physical interaction with UniProtKB:P20353
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Gαi; FB:FBgn0001104
inferred from physical interaction with FLYBASE:mud; FB:FBgn0002873
Terms Based on Predictions or Assertions (0 terms)
Biological Process (7 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
-
Summaries
Summary (Interactive Fly)

involved in asymmetric cell divisions and establishment of cell polarity - Prefoldin acts synergistically with Pins to regulate asymmetric division of both neuroblasts and intermediate neural progenitors

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\pins for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0085189
3279
658
Additional Transcript Data and Comments
Reported size (kB)

3.3 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084559
71.5
658
6.73
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

658 (aa); 72 (kD predicted)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pins using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

raps is observed as a crescent in the apical cortex in neuroblasts after delamination. It is also observed in cortical crescents in the dividing cells of procephalic mitotic domain 9.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\pins in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 35 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of pins
Transgenic constructs containing regulatory region of pins
Deletions and Duplications ( 5 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
anterior fascicle & synapse, with Scer\GAL4elav-C155
anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155
embryonic neuroblast & spindle
larval brain & neuroblast
mitotic cell cycle & spindle
mitotic domain 9 & epithelial cell
mitotic domain 9 & spindle
spindle & external sensory organ precursor cell I
spindle & larval neuroblast
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
12 of 15
No
Yes
2 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
 
3  
12 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
10 of 13
Yes
Yes
9 of 13
No
Yes
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
8 of 12
Yes
Yes
7 of 12
No
Yes
2 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
12 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
13 of 15
Yes
Yes
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091904T9 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915012P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00VJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05CB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G04C9 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Dmel gene
Ortholog showing functional complementation
Supporting References
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map

3-94

Cytogenetic map
Sequence location
3R:27,582,840..27,586,376 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
98A8-98A8
Limits computationally determined from genome sequence between P{PZ}sda03884 and P{PZ}btzrL203
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (13)
Genomic Clones (20)
cDNA Clones (126)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: pins raps

    Source for database merge of

    Source for merge of: Rad pins

    Source for merge of: raps CG5692

    Additional comments

    'raps' renamed to 'pins' based on clear preference for the latter over the former in the published literature, and at the request of author O'Kane who first characterized and named the gene in FBrf0128604.

    Other Comments

    baz, raps and dlg1 establish and maintain planar polarity in pI cells.

    raps is involved in the control of asymmetric divisions of neuroblasts.

    In raps mutants, the asymmetric divisions of neuroblasts are altered; insc protein is no longer asymmetrically localized in the dividing neuroblast and the neuroblast division produces two daughter cells of similar sizes. However, the morphologically symmetrical divisions of raps mutant neuroblasts still lead to daughter cells with different fates, as shown by differences in gene expression.

    raps and insc might orient asymmetric cell divisions by localising or locally modulating a heterotrimeric G-protein signalling cascade at the apical cell cortex.

    Identification: identified as a protein that binds to insc in a yeast two-hybrid screen.

    raps is required for all aspects of insc function.

    raps is required for insc protein to asymmetrically localise.

    Isolated using a yeast two-hybrid system.

    raps encodes a putative SH3 binding protein, that co-localises with dlg1 at septate junctions.

    Origin and Etymology
    Discoverer
    Etymology

    The raps protein contains 7 tetratricopeptide repeats which are similar to those existing in "rapsyn", a mouse protein, hence the name "rapsynoid". However, raps is not a homologue of rapsyn.

    Identification
    External Crossreferences and Linkouts ( 46 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (19)
    Reported As
    Symbol Synonym
    Pins
    (Aguilar-Aragon et al., 2020, Magnaghi-Jaulin et al., 2019, Carmena, 2018, Dewey and Johnston, 2017, Lang and Munro, 2017, Richardson and Portela, 2017, Ajduk and Zernicka-Goetz, 2016, Moreira and Morais-de-Sá, 2016, Yasugi and Nishimura, 2016, Zhang et al., 2016, Bajaj et al., 2015, Bell et al., 2015, Hanlon and Andrew, 2015, Mauri et al., 2014, Tipping and Perrimon, 2014, Bergstralh et al., 2013, Chai et al., 2013, Culurgioni and Mapelli, 2013, Lu and Johnston, 2013, Lu and Prehoda, 2013, Andersen et al., 2012, Guilgur et al., 2012, Homem and Knoblich, 2012, Kelsom and Lu, 2012, Smith and Prehoda, 2011, Wee et al., 2011, Cabernard et al., 2010, Januschke and Gonzalez, 2010, Cabernard and Doe, 2009, Gomes et al., 2009, Johnston et al., 2009, Kopein and Katanaev, 2009, Mummery-Widmer et al., 2009, Sahota et al., 2009, Boone and Doe, 2008, Chia et al., 2008, Krahn and Wodarz, 2008, Lin, 2008, Speicher et al., 2008, Yamashita and Fuller, 2008, Slack et al., 2007, Smith et al., 2007, Sommer et al., 2007, Bowman et al., 2006, Hutterer et al., 2006, Katanaev and Tomlinson, 2006, Lee et al., 2006, Lee et al., 2006, Ahringer, 2005, Budde, 2005, David et al., 2005, Gomes and Schweisguth, 2005, Hampoelz et al., 2005, Izumi et al., 2005, Jafar-Nejad et al., 2005, Siegrist and Doe, 2005, Wang et al., 2005, Yamashita et al., 2005, Yu et al., 2005, Bardin et al., 2004, Bellaiche et al., 2004, Betschinger and Knoblich, 2004, Dunlop et al., 2004, Hampoelz and Knoblich, 2004, Izumi et al., 2004, Roegiers and Jan, 2004, Siegrist and Doe, 2004, Torban et al., 2004, Blumer et al., 2003, Hidalgo and ffrench-Constant, 2003, Izumi et al., 2003, Rolls et al., 2003, Rothenberg et al., 2003, Schweisguth et al., 2003, Wodarz and Huttner, 2003, Gonczy, 2002, Le Borgne et al., 2002, Bellaiche et al., 2001, Cai et al., 2001, Dawes-Hoang and Wieschaus, 2001, Doe, 2001, Knust, 2001, Ohno, 2001, Roegiers et al., 2001, Schaefer et al., 2001, Schaefer et al., 2001, Schaefer et al., 2000)
    pins
    (Finegan et al., 2019, Franco and Carmena, 2019, Hu and Jasper, 2019, Pham et al., 2019, Saito et al., 2018, Banerjee et al., 2017, Roubinet et al., 2017, Transgenic RNAi Project members, 2017-, Tsankova et al., 2017, Bosveld et al., 2016, Cate et al., 2016, Zhang et al., 2016, Bell et al., 2015, Bergstralh et al., 2015, Besson et al., 2015, Keder et al., 2015, Schweisguth, 2015, Poulton et al., 2014, Bergstralh et al., 2013, Januschke et al., 2013, Jauffred et al., 2013, Andersen et al., 2012, Carney et al., 2012, Guilgur et al., 2012, Yoshiura et al., 2012, Furman and Bukharina, 2011, Miles et al., 2011, Wang et al., 2011, Chang et al., 2010, Janic et al., 2010, Januschke and Gonzalez, 2010, Ségalen et al., 2010, Bonaccorsi et al., 2007, Nipper et al., 2007, Rebollo et al., 2007, Tountas and Fortini, 2007, Izumi et al., 2006, Lee et al., 2006, Siller et al., 2006, Wang et al., 2006, Wang et al., 2006, Wodarz and Gonzalez, 2006, Budde, 2005, Caussinus and Gonzalez, 2005, David et al., 2005, Jafar-Nejad et al., 2005, Langevin et al., 2005, Yu et al., 2005, Lee et al., 2004, Ahringer, 2003, Albertson and Doe, 2003, Cai et al., 2003, Fuse et al., 2003, Le Borgne and Schweisguth, 2003, Radovic and Bryant, 2003, Tuxworth and Chia, 2003, Wallenfang and Matunis, 2003, Yu et al., 2003, Yu et al., 2003, Adler, 2002, Chia and Yang, 2002, Kaltschmidt and Brand, 2002, Rath et al., 2002, Yu et al., 2002, Bellaiche et al., 2001, Chase, 2001, Doe, 2001, Doe and Bowerman, 2001, Gulli and Peter, 2001, Kraut et al., 2001, Kraut et al., 2001, Parmentier et al., 2001, Schaefer and Knoblich, 2001, Schaefer et al., 2001, Tio et al., 2001, Wodarz, 2001, Brazil and Hemmings, 2000, Jan and Jan, 2000, Leevers, 2000, Ohshiro et al., 2000, Peng et al., 2000, Schaefer, 2000.3.6, Schaefer et al., 2000, Schweisguth, 2000, Yu, 2000.1.31, Yu et al., 2000, Yu et al., 2000)
    Secondary FlyBase IDs
    • FBgn0020384
    • FBgn0039526
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (257)