Gene Dmel\larp

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General Information

Symbol

Dmel\larp

Species

D. melanogaster

Name

La related protein

Annotation symbol

CG14066

Feature type

protein_coding_gene

FlyBase ID

FBgn0040108

Created / Updated

2003-12-22/2003-12-22

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

98C3-98C4

Sequence location

3R:24,143,896..24,154,005 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene La related protein is referred to in FlyBase by the symbol larp (CG14066, FBgn0040108). It has the cytological map location 98C3-98C4. Its sequence location is 3R:24143896..24154005. Its molecular function is unknown. It is involved in the biological processes: centrosome separation; mitotic chromosome condensation; autophagic cell death; salivary gland cell autophagic cell death; spindle assembly. 13 alleles are reported. The phenotypes of these alleles are annotated with: nucleus; spindle; condensed nuclear chromosome; centrosome; mitotic anaphase. It has 3 annotated transcripts and 3 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98C3-98C4

Limits computationally determined from genome sequence between P{PZ}btz^rL203 and P{PZ}l(3)06487⁰⁶⁴⁸⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

98C-98C

(Chauvet, 2000.1.5)

Experimentally Determined Recombination Data

Location

(Horn et al., 2003)

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\larp for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

larp-RA

FBtr0089680

NM_080259

6733

965

larp-RB

FBtr0089682

NM_170366

6749

965

larp-RC

FBtr0089681

NM_170365

6749

1403

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

larp-PA

FBpp0088622

106.2

965

9.12

NP_524998

AAF56783

larp-PB

FBpp0088624

106.2

965

9.12

NP_733245

AAN14139

larp-PC

FBpp0088623

150.9

1403

10.06

NP_733244

AAN14138

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

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FBgn0040108

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein of unknown function DM15 (IPR006607)

RNA-binding protein Lupus La (IPR006630)

Winged helix repressor DNA-binding (IPR011991)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

11115

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI542886
AI533545

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0040108

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

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FBgn0040108

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

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FBgn0040108

BDGP expression data

•

Show all BDGP original images, annotations and embryonic microarray data for larp

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for larp

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

larp^CC06230

lethal | recessive

larp⁴³

Other Phenotypes

Allele

mitotic cell cycle defective

larp¹

Phenotype manifest in

Allele

nucleus

larp¹

spindle

larp¹

condensed nuclear chromosome

larp¹

centrosome

larp¹

mitotic anaphase

larp¹

Classical Alleles ( 12 )

For All Classical Alleles Show

Allele of larp	Mutagen	Stocks	Known lesion
larp¹		0	--
larp⁴³	piggyBac transposase	0	Yes
larp^CC06230	P-element activity	0	Yes
larp^GS8036		0	--
larp^Wee-P5		0	Yes
larp^ZCL2726	P-element activity	1	--
larp^d00350	P-element activity	1	--
larp^e00508	piggyBac transposase	1	--
larp^f01093	piggyBac transposase	1	--
larp^f01481	piggyBac transposase	1	--
larp^f01612	piggyBac transposase	0	--
larp^f01877	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of larp	Class	Mutagen	Stocks	Known lesion
larp^GD8215		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful duplication

Dp(3;3)501

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD8215}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{PTT-GC}larp^CC06230

P{PTT-GC}larp^ZCL2726

P{Wee-P.ph0}larp^Wee-P5

insertion of enhancer trap binary system

PBac{GAL4D,EYFP}larp⁴³

insertion of enhancer trap

P{lacW}l(3)SH082^SH082

insertion of mobile activating element

P{GSV3}CG14065^GS8036

P{XP}larp^d00350

P{XP}larp^d07135

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 6 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

centrosome separation

inferred from mutant phenotype

(Blagden et al., 2005)

mitotic chromosome condensation

inferred from mutant phenotype

(Blagden et al., 2005)

salivary gland cell autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

spindle assembly

inferred from mutant phenotype

(Blagden et al., 2005)

Cellular Component

CV term

Evidence

References

cytosol

inferred from direct assay

(Clyne et al., 2003)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

larp allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

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72798

Orthologs

Genome-wide drosophilid orthologs

Dere\GG12072

Dmoj\GI24848

Dper\GL23866

Dpse\GA27083

Dvir\GJ24490

Dyak\GE10516

Curated drosophilid orthologs

Dpse\GA12737

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000019588
Anopheles gambiae (Ensembl)
ENSBTAP00000011109
Bos taurus (Ensembl)
ENSCAFP00000026016
Canis familiaris (Ensembl)
ENSCINP00000006261
Ciona intestinalis (Ensembl)
NEWSINFRUP00000134843
Takifugu rubripes (Ensembl)
NEWSINFRUP00000147306
Takifugu rubripes (Ensembl)
ENSGALP00000006364
Gallus gallus (Ensembl)
ENSP00000285894
Homo sapiens (Ensembl)
ENSMMUP00000012318
Macaca mulatta (Ensembl)
ENSMODP00000010779
Monodelphis domestica (Ensembl)
ENSPTRP00000047060
Pan troglodytes (Ensembl)
ENSRNOP00000037598
Rattus norvegicus (Ensembl)
GSTENP00029916001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000018431
Xenopus tropicalis (Ensembl)
Q5NBG8
Oryza sativa (Gramene)
AT5G21160.1
Arabidopsis thaliana (Arabidopsis)
WBGene00020097
Caenorhabditis elegans (Wormbase)
ZDB-GENE-030131-5386
Danio rerio (Zfin)
DDB0217890
Dictyostelium discoideum (Dictybase)
MGI:1890165
Mus musculus (Informatics)
SPAC1527.03
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 9 )

P{lacW}l(3)SH082^SH082

VDRC

v24841

w¹¹¹⁸; P{GD8215}v24841

v24840

w¹¹¹⁸; P{GD8215}v24840/CyO

Bloomington

6830

w¹¹¹⁸; P{PTT-GC}larp^ZCL2726/TM3, Sb¹

Genomic Clones ( 2 )

cDNA Clones ( 34 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

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FBgn0040108

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

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FBgn0040108

Antibody Information