FlyBase Gene Report: Dmel\cmet

KINESINS -: Kinesin superfamily proteins (KIFs) are microtubule motor proteins which use the hydrolysis of ATP to drive directional movement along microtubules. KIFs possess a well-conserved 360 residue globular head domain which binds and hydrolyses ATP and interacts with microtubules. Many KIFs homodimerize via coiled-coil interactions in the stalk region. KIFs bind cargo through their variable tail regions and are involved in transporting organelles, protein complexes, mRNAs and the movement of spindles and chromosomes during cell division. (Adapted from FBrf0219884).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\cmet or the JBrowse view of Dmel\cmet for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

cmet-RC

FBtr0332202

NM_080254

7008

2189

cmet-RD

FBtr0332203

NM_001273451

6843

2134

cmet-RE

FBtr0332204

NM_001273452

6999

2186

cmet-RF

FBtr0332205

NM_001273453

6837

2132

Additional Transcript Data and Comments

Reported size (kB)

7.524 (longest cDNA)

(Yucel et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

cmet-PC

FBpp0304511

251.5

2189

4.84

NP_524993

AAF53088

cmet-PD

FBpp0304512

245.0

2134

4.87

NP_001260380

AGB92915

cmet-PE

FBpp0304513

251.2

2186

4.83

NP_001260381

AGB92916

cmet-PF

FBpp0304514

244.8

2132

4.87

NP_001260382

AGB92917

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

2244 (aa); 250, 180 (kD observed); 257 (kD predicted)

(Yucel et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\cmet using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cmet_1

Linkouts

Gene Ontology (12 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR001752, InterPro:IPR019821

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

ATP-dependent microtubule motor activity, plus-end-directed

inferred from sequence or structural similarity with HGNC:1856

(Yucel et al., 2000)

ATPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

microtubule binding

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

microtubule motor activity

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

mitotic metaphase plate congression

inferred from mutant phenotype

(Yucel et al., 2000)

regulation of cell cycle

inferred from mutant phenotype

(Clemente-Ruiz et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

microtubule-based movement

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

mitotic metaphase plate congression

inferred from biological aspect of ancestor with PANTHER:PTN001947122

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

kinetochore

inferred from direct assay

(Yucel et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

condensed chromosome outer kinetochore

non-traceable author statement

(Blower and Karpen, 2001)

kinesin complex

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

microtubule

inferred from biological aspect of ancestor with PANTHER:PTN000648413

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

cmet associates with centromeric regions of chromosomes, probably kinetochores.

(Yucel et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

kinetochore

inferred from direct assay

(Yucel et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\cmet in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\cmet

View exonic expression by tissue for Dmel\cmet

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040232

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6392-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0040232

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of cmet

Transgenic constructs containing regulatory region of cmet

Deletions and Duplications ( 9 )

Disrupted in

Df(2L)ab-c1

(Sawamura et al., 2010)

Df(2L)BSC236

(Christensen and Cook, 2006.12.5)

Df(2L)BSC241

(Christensen and Cook, 2006.12.5)

Df(2L)BSC242

(Christensen and Cook, 2008.4.15)

Df(2L)BSC343

(Christensen and Cook, 2007.10.29)

Df(2L)Exel6028

(Ryder, 2004.4.26)

Df(2L)Prl

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2L)salm^FRT

(Spletter et al., 2015)

Partially disrupted in

Df(2L)Cenp-E141

(Radford et al., 2015)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

cmet^GD11911

lethal (with Df(2L)Cenp-E141)

cmet^Δ

lethal | embryonic stage | maternal effect

cmet⁰⁴⁴³¹

lethal | recessive

cmet⁰⁴⁴³¹

lethal - all die before end of larval stage | recessive

cmet⁰⁴⁴³¹

viable

cmet^EY00718

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

cmet^GD11911

viable, with Scer\GAL4^Mef2.PR

cmet^GD11911

viable, with Scer\GAL4^pnr-MD237

cmet^GD11911

viable, with Scer\GAL4^tin.CΔ4

cmet^GD11911

Sterility

Allele

fertile

cmet^EY00718

Other Phenotypes

Allele

decreased cell number | germline clone | oogenesis

cmet^Δ

mitotic cell cycle defective

cmet^Δ

mitotic cell cycle defective | recessive

neuroanatomy defective | larval stage

cmet^GD11911

size defective | adult stage

size defective | adult stage | conditional

size defective | adult stage | heat sensitive

some die during larval stage | recessive

cmet⁰⁴⁴³¹

visible, with Scer\GAL4^pnr-MD237

cmet^GD11911

visible | adult stage

visible | adult stage | conditional

visible | adult stage | heat sensitive

Phenotype manifest in

Allele

centrosome | germline clone

cmet^1x1

chromosome, with Scer\GAL4^{VP16.mat.αTub67C}

cmet^GL00404

condensed nuclear chromosome

cmet^Δ

embryo | germline clone | maternal effect

cmet^Δ

embryonic/first instar larval cuticle | maternal effect

cmet⁰⁴⁴³¹

embryonic/larval brain | larval stage

cmet^1x1

eye

eye | conditional

eye | heat sensitive

ganglion mother cell | larval stage

cmet^GD11911

karyosome, with Scer\GAL4^{VP16.mat.αTub67C}

cmet^GL00404

mitosis & nuclear chromosome

mitosis & nuclear chromosome | germ-line clone

mitotic anaphase | germline clone

mitotic cell cycle

mitotic metaphase | germline clone

spermatocyte

cmet^Δ

trichogen cell, with Scer\GAL4^pnr-MD237

cmet^GD11911

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (26)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CENPE

8 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\KIF27

1 of 15

Hsap\MORC3

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (25)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cenpe

8 of 15

Yes

Mmus\Kif5a

2 of 15

Mmus\Kif5c

2 of 15

Mmus\Cenpf

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Kif27

1 of 15

Mmus\Morc3

1 of 15

Yes

Rattus norvegicus (Norway rat) (18)

Rnor\Cenpe

8 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

Yes

Rnor\Golgb1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Rnor\Kif27

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\cenpe

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Danio rerio (Zebrafish) (26)

Drer\cenpe

8 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\si:cabz01066312.1

1 of 15

Yes

Drer\si:dkey-26i13.8

1 of 15

Drer\znf185

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\unc-116

2 of 15

Yes

Cele\anc-1

1 of 15

Cele\che-10

1 of 15

Cele\F59A2.2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (24)

Atha\AT3G10180

5 of 9

Yes

Atha\ZCF125

4 of 9

Yes

Atha\AT3G51150

2 of 9

Yes

Atha\AT4G24170

2 of 9

Yes

Atha\AT5G66310

2 of 9

Yes

Atha\CIP1

2 of 9

Yes

Atha\HIK

2 of 9

Yes

Atha\TES

2 of 9

Yes

Atha\AT1G21730

1 of 9

Yes

Atha\AT2G21300

1 of 9

Yes

Atha\AT2G21380

1 of 9

Yes

Atha\AT2G32240

1 of 9

Atha\AT3G44050

1 of 9

Atha\AT4G38950

1 of 9

Yes

Atha\AT4G39050

1 of 9

Yes

Atha\AT5G06670

1 of 9

Yes

Atha\AT5G40450

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

Yes

Atha\PAKRP1

1 of 9

Atha\POK1

1 of 9

Atha\POK2

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\KIP2

3 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\alm1

2 of 12

Yes

Spom\spo15

2 of 12

Yes

Spom\klp3

1 of 12

Spom\nup211

1 of 12

Spom\tea2

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900WF )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\cana

Drosophila melanogaster

fruit fly

Dmel\cmet

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00001915

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila simulans

Drosophila sechellia

Drosophila sechellia

Drosophila erecta

Drosophila erecta

Drosophila yakuba

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25699

Drosophila persimilis

Dper\GL18576

Drosophila willistoni

Dwil\GK14900

Drosophila virilis

Dvir\GJ13256

Drosophila mojavensis

Dmoj\GI20661

Drosophila grimshawi

Dgri\GH13389

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503WE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17017423

Glossina morsitans

Tsetse fly

Gmor\GMOY003563

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07584

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014736

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003594

Anopheles darlingi

American malaria mosquito

Adar\ADAC005972

Anopheles gambiae

Malaria mosquito

Agam\AGAP006472

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012220

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W085F )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000862

Heliconius melpomene

Postman butterfly

Hmel\HMEL010509

Apis florea

Little honeybee

Aflo\2147570339

Apis florea

Little honeybee

Aflo\3440415092

Apis mellifera

Western honey bee

Amel\Ame.17218

Apis mellifera

Western honey bee

Amel\GB51255

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20104

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20075

Bombus terrestris

Buff-tailed bumblebee

Bter\15205923

Bombus terrestris

Buff-tailed bumblebee

Bter\15205943

Bombus terrestris

Buff-tailed bumblebee

Bter\15205926

Linepithema humile

Argentine ant

Lhum\LH25884

Linepithema humile

Argentine ant

Lhum\LH25507

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00068b

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001d47

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG037139

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05202

Tribolium castaneum

Red flour beetle

Tcas\TC014111

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI22614

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI010110

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01338

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0817 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011569

Ixodes scapularis

Black-legged tick

Isca\ISCW023750

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62031.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_300890

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_41086

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304777

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0CFT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Cenpe

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-CenpeL1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000012785

Gallus gallus

Domestic chicken

Ggal\CENPE

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (28)

Gene Symbol

Score

Source

Alignment

4 of 10

Khc

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

nod

2 of 10

unc-104

2 of 10

CG14535

1 of 10

CG32318

1 of 10

cos

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

lva

1 of 10

mud

1 of 10

ncd

1 of 10

neb

1 of 10

pav

1 of 10

sub

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

cancer, models of chromosomal instability

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

cmet^NIG.6392R

model of cancer

CEA with BacA\p35^UAS.cUa

(Clemente-Ruiz et al., 2014)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CENPE; centromere protein E

model of primary autosomal recessive microcephaly 13

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CENPE; centromere protein E

8 of 15

117143

MICROCEPHALY 13, PRIMARY, AUTOSOMAL RECESSIVE; MCPH13

primary autosomal recessive microcephaly 13

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

cmet - Cen

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Fgop2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Ndc80

anti tag coimmunoprecipitation, peptide massfingerprinting

(Przewloka et al., 2007)

cmet - Panx

experimental knowledge based

(Guruharsha et al., 2011)

cmet - RAF2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Slmap

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Tlk

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Ubqn

experimental knowledge based

(Guruharsha et al., 2011)

cmet - Zw10

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

cmet - Zwilch

anti bait coimmunoprecipitation, peptide massfingerprinting

(Williams et al., 2003)

cmet - dgt6

anti bait coimmunoprecipitation, western blot

(Bucciarelli et al., 2009)

cmet - dup

experimental knowledge based

(Guruharsha et al., 2011)

cmet - geminin

experimental knowledge based

(Guruharsha et al., 2011)

cmet - nxf2

experimental knowledge based

(Guruharsha et al., 2011)

cmet - row

experimental knowledge based

(Guruharsha et al., 2011)

cmet - woc

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cmet

enhanceable

Ndc80

(Radford et al., 2015)

cmet

suppressible

Spc105R

(Radford et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72792

DroID - A comprehensive database of gene and protein interactions.

FBgn0040232

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53561

MIST (protein-protein) - An integrated Molecular Interaction Database

cmet

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-44

Cytogenetic map

32E2-32E2

Sequence location

2L:11,261,026..11,269,002 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32E2-32E2

Limits computationally determined from genome sequence between P{PZ}l(2)04008⁰⁴⁰⁰⁸&P{PZ}cmet⁰⁴⁴³¹ and P{PZ}salm⁰³⁶⁰²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32D-32E

(Yucel, 1999.12.30)

32E1-32E2

(determined by in situ hybridisation)

(Spradling et al., 1999, Perrimon et al., 1996)

32E1-32E2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-44

(FlyBase, 2016)

2-37.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

54004

y¹ v¹; P{TRiP.HMJ21427}attP40

11375

P{PZ}cmet⁰⁴⁴³¹ cn¹/CyO; ry⁵⁰⁶

32327

y¹ w^*; cmet^Δ/CyO, y⁺

19807

y¹ w^67c23; P{EPgy2}cmet^EY00718

35816

y¹ sc^* v¹ sev²¹; P{TRiP.GL00404}attP2

VDRC

v330414

P{VSH330414}attP40

v35081

w¹¹¹⁸; P{GD11911}v35081

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040232

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040232

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53561

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Yucel et al., 2000)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: cmet CG6392

(Greene, 2000.4.11, FlyBase, 1996-)

Source for merge of: cmet l(2)04431

(Misra, 2000.8.9)

Additional comments

Source for merge of cmet CG6392 was sequence comparison ( date:000725 ).

(FlyBase, 1996-)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cell morphology is aberrant, indicative of a failure in cell cycle/mitosis progression. This phenotype is not seen in Kc167 cells.

(Kiger et al., 2003)

cmet is required for the maintenance of chromosomes at the metaphase plate.

(Yucel et al., 2000)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. cmet gene expression during oogenesis is not critical to embryonic development, but the gene function may be essential for fertilisation and/or completion of meiosis.

(Perrimon et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53561

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040232

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

cmet_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0040232

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53561

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014111

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6392

modMine - A data warehouse for the modENCODE project

FBgn0040232

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Kinesins

Linkouts

BioGRID - A database of protein and genetic interactions.

72792

DroID - A comprehensive database of gene and protein interactions.

FBgn0040232

DRSC - Results frm RNAi screens

FBgn0040232

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040232

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6392-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0040232

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53561

MIST (protein-protein) - An integrated Molecular Interaction Database

cmet

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Kinesins

Synonyms and Secondary IDs (22)

Reported As

Symbol Synonym

CENP-E

(Kondo et al., 2017, Clemente-Ruiz et al., 2014, Morais da Silva et al., 2013)

CENP-E meta

(Brunet and Vernos, 2001)

CENP-META

(Martins et al., 2009)

CENP-Meta

(Kwon et al., 2008)

CENP-meta

(Ye et al., 2015, Feijão et al., 2013, Moutinho-Pereira et al., 2013, Pool et al., 2012, Gerhold et al., 2011, Goshima et al., 2007, Maia et al., 2007, Maiato et al., 2006, Goshima and Vale, 2003)

CENPE

(Morais da Silva et al., 2013)

CENPmeta

(Przewloka et al., 2007)

CG6392

(Kondo et al., 2017, Meadows, 2017.9.14, Lee et al., 2013, Moutinho-Pereira et al., 2013, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Zhang et al., 2010, Hughes et al., 2008, Goshima et al., 2007, Przewloka et al., 2007, Molnar et al., 2006, Mount, 2001.8.14, Goldstein and Gunawardena, 2000, Greene, 2000.4.11)

Cenp-E

(Bucciarelli et al., 2009, Cenci et al., 2008, Musarò et al., 2008, Mentelova et al., 2007)

Cenp-M

(Dzhindzhev et al., 2005)

Cenp-meta

(Resende et al., 2018, Bucciarelli et al., 2009, Cenci et al., 2008)

Cenp_E

(Civelekoglu-Scholey et al., 2006)

Cmet

(Hirokawa, 2012.11.20, Miki et al., 2005, Miki et al., 2001)

DmCmet

(Hirokawa, 2012)

DmCmeta

(Dagenbach and Endow, 2004, Dagenbach and Endow, 2004, Kim and Endow, 2000)

KIF 10

(Miki et al., 2001)

KIF10

(Hirokawa, 2012.11.20)

cmet

(Rotelli et al., 2019, Radford et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Wainman et al., 2012, Gerhold et al., 2011, Neumüller et al., 2011, Sawamura et al., 2010, Rahmani et al., 2009, Wainman et al., 2009, Christensen and Cook, 2008.4.15, Christensen and Cook, 2007.10.29, Georlette et al., 2007, Christensen and Cook, 2006.12.5, Christensen and Cook, 2006.12.5, Foglietti et al., 2006, Molnar et al., 2006, Sawamura and Yamamoto, 2004)

l(2)04431

(Spradling et al., 1999)

Name Synonyms

CENP-meta

(Hirokawa, 2012.11.20, Gerhold et al., 2011, Przewloka et al., 2011, Fisher et al., 2008, Sawamura and Yamamoto, 2004, Yucel, 1999.12.30)

Cenp-meta

(Musarò et al., 2008, Dzhindzhev et al., 2005)

CenpMeta

(Karess, 2005)

Kinesin2A

(Kollmar, 2015.9.15)

cenp-meta

(Rubin et al., 2014, Wainman et al., 2012, Wainman et al., 2009, Williams et al., 2007)

Secondary FlyBase IDs

FBgn0010574
FBgn0032355

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (97)

Report Sections

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