FlyBase Gene Report: Dmel\Jafrac1

General Information

Symbol

Dmel\Jafrac1

Species

D. melanogaster

Name

thioredoxin peroxidase 1

Annotation Symbol

CG1633

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040309

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DPx-4783, DPx4783, dPrx1, Jafrac, Peroxiredoxin 1

Key Links

Genomic Location

Cytogenetic map

11E11-11E11

Sequence location

X:13,329,824..13,331,994 [+]

Recombination map

1-42

RefSeq locus

NC_004354 REGION:13329824..13331994

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables thioredoxin peroxidase activity

inferred from direct assay

(assigned by UniProt )

(Bauer et al., 2002, Radyuk et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables thioredoxin peroxidase activity

non-traceable author statement

(assigned by UniProt )

(Rodriguez et al., 2000)

Biological Process (11 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cell adhesion

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in cell redox homeostasis

inferred from direct assay

(assigned by UniProt )

(Bauer et al., 2002)

involved_in determination of adult lifespan

inferred from mutant phenotype

(Biteau et al., 2010)

involved_in germ cell development

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in germ cell migration

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in hydrogen peroxide catabolic process

inferred from direct assay

(Radyuk et al., 2001)

involved_in response to oxidative stress

inferred from mutant phenotype

(Degennaro et al., 2011)

involved_in response to starvation

inferred from mutant phenotype

(Biteau et al., 2010)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cell redox homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in leukocyte activation

inferred from biological aspect of ancestor with PANTHER:PTN001641775

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in oxidation-reduction process

inferred from electronic annotation with InterPro:IPR000866, InterPro:IPR019479

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

involved_in removal of superoxide radicals

inferred from biological aspect of ancestor with PANTHER:PTN000743557

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(assigned by UniProt )

(Radyuk et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000073874

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

THIOREDOXIN PEROXIDASES

PEROXIREDOXINS

Protein Family (UniProt)

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily. (Q9V3P0)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

THIOREDOXIN PEROXIDASES -: Thioredoxin peroxidases oxidize thoredoxin with the reduction of hydrogen peroxide.
PEROXIREDOXINS -: Peroxiredoxins are a family of thiol-specific antioxidant proteins that catalyze the reduction of hydrogen peroxide, peroxynitrite and a wide range of organic hydroperoxides (ROOH) to corresponding alcohol (ROH) and water. Catalytic activity is dependent on the oxidation of a conserved cysteine residue at the active site. (Adapted from PMID:12517450).

Protein Function (UniProtKB)

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2) (By similarity). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation (By similarity).

(UniProt, Q9V3P0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Jafrac1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Jafrac1-RA

FBtr0073764

NM_058162

1010

194

Jafrac1-RB

FBtr0073763

NM_167359

1014

194

Jafrac1-RC

FBtr0339699

NM_001298273

1051

194

Jafrac1-RD

FBtr0339700

NM_001298274

1080

194

Jafrac1-RE

FBtr0345161

NM_001298275

976

194

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Jafrac1-PA

FBpp0073595

21.7

194

5.34

NP_477510

AAF48253

Jafrac1-PB

FBpp0073594

21.7

194

5.34

NP_727689

AAF48254

Jafrac1-PC

FBpp0308756

21.7

194

5.34

NP_001285202

AHN59672

Jafrac1-PD

FBpp0308757

21.7

194

5.34

NP_001285203

AHN59673

Jafrac1-PE

FBpp0311371

21.7

194

5.34

NP_001285204

AHN59674

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

194 aa isoforms: Jafrac1-PA, Jafrac1-PB, Jafrac1-PC, Jafrac1-PD, Jafrac1-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer; disulfide-linked, upon oxidation. 5 homodimers assemble to form a ring-like decamer (By similarity). Interacts with GDPD5; forms a mixed-disulfide with GDPD5 (By similarity). Interacts with SESN1 and SESN2 (By similarity).

(UniProt, Q9V3P0)

Post Translational Modification

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

Conjugated to URM1, a ubiquitin-like protein.

(UniProt, Q9V3P0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Jafrac1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

germline cell

(Fisher et al., 2012)

lymph gland primordium

Comment: reported as lymph gland specific anlage

(Fisher et al., 2012)

procrystal cell

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic precursor of adult hindgut

(Fisher et al., 2012)

crystal cell

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

embryonic/larval corpus allatum

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism | ubiquitous | uniform

Comment: maternally deposited

(Degennaro et al., 2011)

embryonic stage 6

primordial germ cell

(Degennaro et al., 2011)

embryonic stage 9

germline cell

(Degennaro et al., 2011)

embryonic stage 14

crystal cell

(Degennaro et al., 2011)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | intense

(Radyuk et al., 2001)

third instar larval stage

organism

(Radyuk et al., 2001)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Lee et al., 2009)

(Lee et al., 2009)

(Lee et al., 2009)

third instar larval stage

embryonic/larval brain

(Lee et al., 2009)

imaginal disc

(Lee et al., 2009)

embryonic/larval Malpighian tubule

(Lee et al., 2009)

Additional Descriptive Data

Jafrac1 transcript is maternally deposited. Maternal transcript quickly degrades, except in germline cells, where it is protected from degradation through embryonic stage 9. At later embryonic stages, Jafrac1 transcript is expressed in crystal cells.

(Degennaro et al., 2011)

Jafrac1 transcripts are detected throughout deveopment by RT-PCR and are abundant in third instar larval brain, imaginal discs and Malpighian tubules.

(Lee et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 5

somatic cell

• cytosol

(Degennaro et al., 2011)

primordial germ cell

• cytosol

(Degennaro et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

day 1 of adulthood & male -- day 2 of adulthood & male

adult thorax

• cytosol

(Radyuk et al., 2001)

embryonic stage -- adult stage

organism

(Radyuk et al., 2001)

Additional Descriptive Data

Jafrac1 is present throughout blastoderm embryos in both somatic cells and germline precursor cells.

(Degennaro et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytosol

inferred from direct assay

(assigned by UniProt )

(Radyuk et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Jafrac1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Jafrac1

View exonic expression by tissue for Dmel\Jafrac1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040309

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040309

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1633-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1633

Flygut - An atlas of the Drosophila adult midgut

FBgn0040309

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Jafrac1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Jafrac1

Transgenic constructs containing regulatory region of Jafrac1

Deletions and Duplications ( 2 )

Disrupted in

Df(1)Exel6245

(Ryder, 2004.4.26)

Not disrupted in

Df(1)f08066-f07791

(Casad et al., 2011)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

cell adhesion defective

cell adhesion defective (with Jafrac1^f08066)

Jafrac1^KG05372

cell adhesion defective (with Jafrac1^KG05372)

Jafrac1^f08066

cell migration defective

cell migration defective (with Jafrac1^f08066)

Jafrac1^KG05372

cell migration defective (with Jafrac1^KG05372)

Jafrac1^f08066

chemosensitive behavior defective, with Scer\GAL4^da.PU

Jafrac1^UAS.cHa

long lived, with Scer\GAL4^NP5130

Jafrac1^UAS.cBa

oxidative stress response defective

Jafrac1^KG05372

oxidative stress response defective | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

Jafrac1^UASp.cDa

short lived | conditional, with Scer\GAL4^da.G32

Jafrac1^UAS.cHa

starvation stress response defective

Jafrac1^KG05372

starvation stress response defective, with Scer\GAL4^NP5130

Jafrac1^UAS.cBa

Phenotype manifest in

Allele

germline cell | maternal effect

germline cell | maternal effect (with Jafrac1^f08066)

Jafrac1^KG05372

germline cell | maternal effect (with Jafrac1^KG05372)

Jafrac1^f08066

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PRDX2

10 of 15

Yes

Hsap\PRDX1

9 of 15

Yes

Hsap\PRDX4

5 of 15

Hsap\PRDX3

4 of 15

Hsap\PRDX6

2 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Prdx2

9 of 15

Yes

Mmus\Prdx1

8 of 15

Yes

5 of 15

4 of 15

2 of 15

1 of 15

Yes

Mmus\Prdx6b

1 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Prdx2

9 of 13

Yes

Rnor\Prdx1

8 of 13

Yes

Rnor\Prdx4

5 of 13

Rnor\Prdx3

4 of 13

Rnor\Prdx6

2 of 13

Xenopus tropicalis (Western clawed frog) (5)

Xtro\prdx2

7 of 12

Yes

Xtro\prdx1

5 of 12

Yes

Xtro\prdx4

4 of 12

Xtro\prdx3

3 of 12

Xtro\prdx6

1 of 12

Danio rerio (Zebrafish) (5)

Drer\prdx2

10 of 15

Yes

Drer\prdx1

6 of 15

Yes

Drer\prdx3

4 of 15

Drer\prdx4

4 of 15

Drer\prdx6

2 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\prdx-2

10 of 15

Yes

Cele\prdx-3

5 of 15

Cele\prdx-6

2 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\2-Cys Prx B

8 of 9

Yes

Atha\AT3G11630

8 of 9

Yes

Atha\PER1

1 of 9

Atha\PRXQ

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\TSA1

12 of 15

Yes

Scer\TSA2

10 of 15

Yes

Scer\PRX1

2 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\tpx1

10 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190FQJ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14060

Drosophila persimilis

Dper\GL26916

Drosophila willistoni

Dwil\GK25196

Drosophila virilis

Dvir\GJ15754

Drosophila mojavensis

Dmoj\GI16105

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150B60 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17011000

Musca domestica

House fly

Mdoa\17018889

Glossina morsitans

Tsetse fly

Gmor\GMOY000509

Glossina morsitans

Tsetse fly

Gmor\GMOY008040

Glossina morsitans

Tsetse fly

Gmor\GMOY006085

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_04921

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_13857

Mayetiola destructor

Hessian fly

Mdes\Mdes003451

Mayetiola destructor

Hessian fly

Mdes\Mdes003413

Aedes aegypti

Yellow fever mosquito

Aaeg\TPX2

Aedes aegypti

Yellow fever mosquito

Aaeg\TPX3

Anopheles darlingi

American malaria mosquito

Adar\ADAC007846

Anopheles darlingi

American malaria mosquito

Adar\ADAC004923

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ011413

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003399

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0ELF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA002406

Bombyx mori

Silkmoth

Bmor\BGIBMGA002186

Bombyx mori

Silkmoth

Bmor\BGIBMGA000064

Danaus plexippus

Monarch butterfly

Dple\DPOGS210284-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS211437-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS208935-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL003523

Heliconius melpomene

Postman butterfly

Hmel\HMEL015335

Heliconius melpomene

Postman butterfly

Hmel\HMEL004763

Apis florea

Little honeybee

Aflo\4453311364

Apis florea

Little honeybee

Aflo\2824965204

Apis mellifera

Western honey bee

Amel\GB50555

Apis mellifera

Western honey bee

Amel\Tpx-1

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP15926

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP15667

Bombus terrestris

Buff-tailed bumblebee

Bter\15210864

Bombus terrestris

Buff-tailed bumblebee

Bter\15210947

Linepithema humile

Argentine ant

Lhum\LH17181

Linepithema humile

Argentine ant

Lhum\LH21147

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000a1f

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000289

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010912

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG017598

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005664

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07067

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01899

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12823

Tribolium castaneum

Red flour beetle

Tcas\TC001700

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_14929

Tribolium castaneum

Red flour beetle

Tcas\TC001071

Tribolium castaneum

Red flour beetle

Tcas\TC012328

Pediculus humanus

Human body louse

Phum\PHUM569550

Pediculus humanus

Human body louse

Phum\PHUM452340

Pediculus humanus

Human body louse

Phum\PHUM213050

Rhodnius prolixus

Kissing bug

Rpro\RPRC011126

Rhodnius prolixus

Kissing bug

Rpro\RPRC007595

Rhodnius prolixus

Kissing bug

Rpro\RPRC012922

Cimex lectularius

Bed bug

Clec\CLEC001613

Cimex lectularius

Bed bug

Clec\CLEC000824

Cimex lectularius

Bed bug

Clec\CLEC002968

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003960

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003947

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002506

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13882

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_15191

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_13766

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0E1Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005193

Strigamia maritima

European centipede

Smar\SMAR013104

Strigamia maritima

European centipede

Smar\SMAR013788

Ixodes scapularis

Black-legged tick

Isca\ISCW015321

Ixodes scapularis

Black-legged tick

Isca\ISCW013767

Ixodes scapularis

Black-legged tick

Isca\ISCW010532

Stegodyphus mimosarum

African social velvet spider

Smim\KFM66957.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM78263.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58668.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g09470

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g00400

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230422

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_329419

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_305931

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0IE5 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Tpx

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Pxr4

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000019865

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000003002

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000006430

Gallus gallus

Domestic chicken

Ggal\PRDX3

Gallus gallus

Domestic chicken

Ggal\PRDX1

Gallus gallus

Domestic chicken

Ggal\PRDX4

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

7 of 10

7 of 10

6 of 10

3 of 10

3 of 10

3 of 10

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PRDX1; peroxiredoxin 1

model of methylmalonic aciduria and homocystinuria type cblC

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PRDX2; peroxiredoxin 2

10 of 15

600538

PRDX1; peroxiredoxin 1

9 of 15

176763

METHYLMALONIC ACIDURIA AND HOMOCYSTINURIA, cblC TYPE; MAHCC

methylmalonic aciduria and homocystinuria type cblC

PRDX4; peroxiredoxin 4

5 of 15

300927

PRDX3; peroxiredoxin 3

4 of 15

604769

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Jafrac1 - Mekk1

anti tag coimmunoprecipitation, disulfide bond, anti tag western blot

(Barata and Dick, 2020)

Jafrac1 - MtnA

pull down, anti tag western blot

(Tio et al., 2009)

Jafrac1 - MtnB

two hybrid, pull down, anti tag western blot

(Tio et al., 2009)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

gfzf

suppressible

Jafrac1

(Smith et al., 2019)

suppressible

(Nam et al., 2012)

suppressible

(Nam et al., 2012)

enhanceable

(Degennaro et al., 2011)

Urm1

suppressible

Jafrac1

(Khoshnood et al., 2016)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9V3P0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

72802

DroID - A comprehensive database of gene and protein interactions.

FBgn0040309

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53578

MIST (genetic) - An integrated Molecular Interaction Database

Jafrac1

MIST (protein-protein) - An integrated Molecular Interaction Database

Jafrac1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Peroxisome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

TP53 Regulates Metabolic Genes

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-42

Cytogenetic map

11E11-11E11

Sequence location

X:13,329,824..13,331,994 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11E11-11E11

Limits computationally determined from genome sequence between P{EP}EP1461 and P{EP}CG1640^EP1626

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11E-11E

(Santaren, 1999.7.8)

11E6-11E8

(Radyuk, 2000.11.16)

11E-11E

(determined by in situ hybridisation)

(Rodriguez et al., 2000)

Experimentally Determined Recombination Data

Location

1-42

(FlyBase, 2016)

1-42.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

14440

y¹ P{SUPor-P}Jafrac1^KG05372

32498

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00501}attP2

34971

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00935}attP2

38926

y¹ sc^* v¹ sev²¹; P{TRiP.GL01018}attP40

Kyoto

112979

w^* P{GawB}Jafrac1^NP2597 / FM7c

104253

y^* w^* P{GawB}Jafrac1^NP2597 / FM7c

113854

y^* w^* P{GawB}Jafrac1^NP6118 / FM7c

VDRC

v330046

P{VSH330046}attP40

v109514

P{KK110159}VIE-260B

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (137)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040309

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040309

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53578

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Degennaro et al., 2011, Radyuk et al., 2001)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Jafrac1 Prx4783

(Orr, 2001.3.5)

Source for database merge of

Source for merge of: Jafrac1 CG1633

(Bayraktaroglu, 2000.8.7)

Additional comments

The CG6888 gene may have been derived from the Jafrac1 gene by retroposition.

(Dai et al., 2006)

Relationship between "Jafrac1" and "SSP6107" is unclear; they may represent alternative transcripts of the same gene, or two separate genes.

(Rodriguez et al., 2000)

Other Comments

Jafrac1 regulates germ cell internalization during gastrulation.

(Degennaro et al., 2011)

Jafrac1 protects flies from oxidative stress.

(Degennaro et al., 2011)

Jafrac1 is not required for the formation, specification, motility, or survival of primordial germline cells.

(Degennaro et al., 2011)

Trx-2 not dhd is the substrate of Jafrac1.

(Bauer et al., 2002)

Origin and Etymology

Discoverer

Etymology

The gene is named "Jafrac1" after the initials of people in the Santaren research group who first published on the gene.

(Santaren, 2001.10.24)

Identification

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53578

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3P0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JF59

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040309

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040309

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1633

Flygut - An atlas of the Drosophila adult midgut

FBgn0040309

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53578

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1633

modMine - A data warehouse for the modENCODE project

FBgn0040309

Linkouts

BioGRID - A database of protein and genetic interactions.

72802

DroID - A comprehensive database of gene and protein interactions.

FBgn0040309

DRSC - Results frm RNAi screens

FBgn0040309

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0040309

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1633-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040309

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

FlyMine - An integrated database for Drosophila genomics

FBgn0040309

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

53578

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Peroxisome

MIST (genetic) - An integrated Molecular Interaction Database

Jafrac1

MIST (protein-protein) - An integrated Molecular Interaction Database

Jafrac1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

TP53 Regulates Metabolic Genes

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

1633

(Ravi et al., 2009)

DPx-4783

(Radyuk et al., 2003, Orr and Radyuk, 2002, Radyuk et al., 2001)

DPx4783

(Detienne et al., 2018, Heifetz and Rivlin, 2010, Orr et al., 2001)

DmTPx-1

(Bauer et al., 2002)

Dpx-4783

(Lee et al., 2009)

JAFRAC 1

(Abolaji et al., 2015)

Jafrac

(Besson et al., 2015, Gao et al., 2014)

Jafrac1

(Olufs et al., 2020, Vásquez-Procopio et al., 2020, Bradshaw, 2019, Hall et al., 2019, Smith et al., 2019, Anderson-Baron and Simmonds, 2018, Jo and Imm, 2017, Khoshnood et al., 2017, McClelland et al., 2017, Miller et al., 2017, Wang et al., 2017, Du et al., 2016, Khoshnood et al., 2016, Besson et al., 2015, Dent et al., 2015, Izutsu et al., 2015, Kavi et al., 2015, Omelyanchuk et al., 2015, Ugrankar et al., 2015, Ashwal-Fluss et al., 2014, Huang et al., 2014, Luck et al., 2014, Mukherjee et al., 2014, Wang et al., 2014, Colinet et al., 2013, Kockmann et al., 2013, Kwon et al., 2013, Mishra et al., 2013, Sen et al., 2013, Edgar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Albrecht et al., 2011, Degennaro et al., 2011, Hochmuth et al., 2011, Wangler et al., 2011, Biteau et al., 2010, Gallach et al., 2010, Monzo et al., 2010, Müller et al., 2010, Lee et al., 2009, Li et al., 2009, Tio et al., 2009, Curtis et al., 2007, Lee et al., 2007, Stuart et al., 2007, Radyuk et al., 2001)

PRDX1

(Anderson-Baron and Simmonds, 2018)

PRDX1/2/4

(Baron et al., 2016)

PRDX1_DROME

(Fukuyama et al., 2012)

Prx

(Tio et al., 2009)

Prx4783

SSP5111

(Rodriguez et al., 2000)

T20

(Vierstraete et al., 2004)

TPX-1

(Lotz et al., 2014)

cTPx

(Radyuk et al., 2003)

dPrx I

(Chen et al., 2005)

dPrx1

(Bradshaw, 2019, Detienne et al., 2018)

jafrac1

(Nam et al., 2012, Degennaro et al., 2011, Biteau et al., 2010, Lee et al., 2007, Ha et al., 2005, Ha et al., 2005)

jf1

(Degennaro et al., 2011)

Name Synonyms

Jafrac-1

(Kuranaga, 2011)

Jafrac1

(Detienne et al., 2018, Heifetz and Rivlin, 2010)

Peroxiredoxin

(Castillo et al., 2015)

Peroxiredoxin 1

(Anholt and Williams, 2010, Pflieger et al., 2008)

Peroxiredoxin 4783

(Orr et al., 2001)

Peroxiredoxin I

(Chen et al., 2005)

Thioredoxin peroxidase 1

(Anderson-Baron and Simmonds, 2018, Heifetz and Rivlin, 2010, de Morais Guedes et al., 2003)

jafrac1

(Biteau et al., 2011)

peroxidase

(Curtis et al., 2007)

peroxiredoxin

(Tio et al., 2009)

thioredoxin peroxidase

(Zhang et al., 2004)

thioredoxin peroxidase 1

(Ramond et al., 2020, Detienne et al., 2018, Jaspers et al., 2012, Cammarato et al., 2011, Santaren, 1999.7.8)

Secondary FlyBase IDs

FBgn0030472

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (132)

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