FlyBase Gene Report: Dmel\Lztr1

General Information

Symbol

Dmel\Lztr1

Species

D. melanogaster

Name

Leucine zipper like transcription regulator 1

Annotation Symbol

CG3711

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040344

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Summary

Inhibitor of Ras signaling (PubMed:30442766). Acts as a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates ubiquitination of Ras (By similarity). Together with Nf1, plays an important role for normal sleep behavior, mainly during the night (PubMed:32339168). Might affect sleep by modulating GABA signaling in Rdl-expressing neurons (PubMed:32339168). Might play a role in the regulation of brain glycogen metabolism and organismal levels of triglycerides (PubMed:32339168). (UniProt, Q9V410)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

EG:BACR7A4.19

Key Links

Genomic Location

Cytogenetic map

1D2-1D2

Sequence location

X:922,889..927,582 [-]

Recombination map

1-0

RefSeq locus

NC_004354 REGION:922889..927582

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in gamma-aminobutyric acid signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(Maurer et al., 2020)

involved_in negative regulation of Ras protein signal transduction

inferred from mutant phenotype

(assigned by UniProt )

(Bigenzahn et al., 2018)

involved_in positive regulation of glycogen metabolic process

inferred from mutant phenotype

(assigned by UniProt )

(Maurer et al., 2020)

involved_in sleep

inferred from genetic interaction with UniProtKB:O01398

(assigned by UniProt )

(Maurer et al., 2020)

involved_in triglyceride homeostasis

inferred from mutant phenotype

(assigned by UniProt )

(Maurer et al., 2020)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN002355794

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the LZTR1 family. (Q9V410)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, Q9V410)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Lztr1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V410)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.51

Stop-codon suppression (UAG) postulated; FBrf0216884.

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Lztr1-RD

FBtr0331303

NM_001272185

4056

998

Lztr1-RE

FBtr0343361

NM_130513

4335

975

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Lztr1-PD

FBpp0303735

108.9

998

8.11

NP_001259114

AGB94960

Lztr1-PE

FBpp0310018

106.4

975

7.96

NP_569869

AAF45563

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of some BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex.

(UniProt, Q9V410)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Lztr1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG3711_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Lztr1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Lztr1

View exonic expression by tissue for Dmel\Lztr1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31037/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040344

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040344

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040344

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3711

Flygut - An atlas of the Drosophila adult midgut

FBgn0040344

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Lztr1

Transgenic constructs containing regulatory region of Lztr1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(1)Exel6224

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Other Phenotypes

Allele

abnormal circadian rhythm | adult stage

Lztr1^MB12128

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Lztr1^GD4339

abnormal sleep | male

Lztr1^MB12128

abnormal sleep | male, with Scer\GAL4^elav-C155

Lztr1^GD4339

abnormal sleep | male, with Scer\GAL4^nSyb.PS

Lztr1^GD4339

abnormal sleep | male, with Scer\GAL4^Rdl.PS

Lztr1^GD4339

abnormal sleep | male | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

Lztr1^GD4339

decreased sleep

Lztr1^KO

decreased sleep | male | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

Lztr1^GD4339

decreased sleep | male limited, with Scer\GAL4^elav-C155

decreased sleep | recessive

Lztr1^MB12128

hypoactive | adult stage | male limited

Lztr1^MB12128

hypoactive | adult stage | male limited, with Scer\GAL4^elav-C155

visible | adult stage, with Scer\GAL4^Act5C.PI

Phenotype manifest in

Allele

wing vein | ectopic, with Scer\GAL4^Act5C.PI

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\LZTR1

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\KLHDC3

1 of 15

Hsap\KLHDC4

1 of 15

Hsap\RABEPK

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Lztr1

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Lztr1

11 of 13

Yes

Rnor\Klhdc2

1 of 13

Yes

Rnor\RGD1562104

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\lztr1

9 of 12

Yes

Xtro\klhdc2

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\lztr1

9 of 15

Yes

Drer\hcfc1a

1 of 15

Drer\hcfc1b

1 of 15

Drer\klhdc2

1 of 15

Yes

Drer\klhdc4

1 of 15

Drer\rabepk

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\F53E4.1

1 of 15

Yes

Cele\hcf-1

1 of 15

Yes

Cele\herc-1

1 of 15

Yes

Cele\Y50D7A.1

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (4)

Atha\AT1G18610

1 of 9

Yes

Atha\AT1G74150

1 of 9

Yes

Atha\AT2G36360

1 of 9

Yes

Atha\AT4G04670

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\KEL1

3 of 15

Yes

Scer\KEL2

3 of 15

Yes

Scer\KEL3

1 of 15

Scer\MDS3

1 of 15

Yes

Scer\PMD1

1 of 15

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\tea3

2 of 12

Yes

Spom\tea1

1 of 12

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (5)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

Hcf

2 of 10

slim

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

chromosome 22q11.2 deletion syndrome

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

LZTR1; leucine zipper like transcription regulator 1

model of Noonan syndrome 10

IEA

(FlyBase, 2019-)

model of Noonan syndrome 2

IEA

(FlyBase, 2019-)

model of neurilemmomatosis

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LZTR1; leucine zipper like transcription regulator 1

13 of 15

600574

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Lztr1

suppressible

Nf1

(Maurer et al., 2020)

Lztr1

enhanceable

Nf1

(Maurer et al., 2020)

Lztr1

suppressible

Ras85D

(Bigenzahn et al., 2018)

Lztr1

suppressible

Rdl

(Maurer et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nf1

enhanceable

Lztr1

(Maurer et al., 2020)

Ras85D

enhanceable

Lztr1

(Bigenzahn et al., 2018)

Ras85D

suppressible

Lztr1

(Bigenzahn et al., 2018)

Rdl

suppressible

Lztr1

(Maurer et al., 2020)

External Data

Subunit Structure (UniProtKB)

Component of some BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex.

(UniProt, Q9V410 )

Linkouts

BioGRID - A database of protein and genetic interactions.

57605

DroID - A comprehensive database of gene and protein interactions.

FBgn0040344

MIST (protein-protein) - An integrated Molecular Interaction Database

CG3711

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-0

Cytogenetic map

1D2-1D2

Sequence location

X:922,889..927,582 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

1D2-1D2

Limits computationally determined from genome sequence between P{EP}EP1600&P{EP}EP1498 and P{EP}EP1615&P{EP}EP964

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-0

(FlyBase, 2016)

1-0.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29940

Mi{ET1}Lztr1^MB12128 w¹¹¹⁸

33422

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00306}attP2

VDRC

v11164

w¹¹¹⁸; P{GD4339}v11164

v13008

w¹¹¹⁸; P{GD4892}v13008

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (26)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LP01394

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040344

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040344

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31037

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Maurer et al., 2020)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Lztr1 CG3711

(Bigenzahn et al., 2018)

Source for database merge of

Source for merge of: EG:BACR7A4.19 CG3711

(FlyBase, 1996-)

Additional comments

Source for merge of EG:BACR7A4.19 CG3711 was sequence comparison ( date:000425 ).

(FlyBase, 1996-)

Other Comments

Origin and Etymology

Discoverer

Etymology

The gene is named "night owl" based on the phenotype of flies in which its function is knocked down.

(Maurer et al., 2020)

Identification

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31037

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V410

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V410

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V410

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040344

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31037/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040344

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CG3711_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040344

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040344

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3711

Flygut - An atlas of the Drosophila adult midgut

FBgn0040344

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31037

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3711

MARRVEL_MODEL - MARRVEL (model organism gene)

31037

modMine - A data warehouse for the modENCODE project

FBgn0040344

Linkouts

BioGRID - A database of protein and genetic interactions.

57605

DroID - A comprehensive database of gene and protein interactions.

FBgn0040344

DRSC - Results frm RNAi screens

FBgn0040344

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040344

FlyMine - An integrated database for Drosophila genomics

FBgn0040344

MIST (protein-protein) - An integrated Molecular Interaction Database

CG3711

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG3711

(Bigenzahn et al., 2018, Appel et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Jungreis et al., 2011, Ni et al., 2010.12.1, Wasbrough et al., 2010, Grieder et al., 2007, Legube et al., 2006)

EG:BACR7A4.19

(Giot et al., 2003, Benos et al., 2001)

LZTR1

(Maurer et al., 2020, Bigenzahn et al., 2018)

Lztr1

(Parker et al., 2020)

nowl

(Maurer et al., 2020)

Name Synonyms

Leucine zipper like transcription regulator 1

night owl

(Maurer et al., 2020)