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Gene Dmel\BobA

General Information
Symbol	Dmel\BobA	Species	D. melanogaster
Name	Brother of Bearded A	Annotation symbol	CG12487
Feature type	protein_coding_gene	FlyBase ID	FBgn0040487
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	Bob
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	71A2-71A2	Sequence location	3L:14,938,599..14,939,212 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Brother of Bearded A is referred to in FlyBase by the symbol Dmel\BobA (CG12487, FBgn0040487). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: sensory organ precursor cell fate determination; sensory organ development; Notch signaling pathway; cell fate specification. 3 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; wing margin bristle; macrochaeta; tormogen cell; microchaeta. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1628084_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of BobA. Gene sequence location is 3L:14938599..14939212.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	References Egger et al., 2010, Development 137(18): 2981--2987 Sequence features HFA09889
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 71A2-71A2   Limits computationally determined from genome sequence between P{PZ}Mpcp00564 and P{PZ}l(3)rO220rO220
Experimentally Determined Cytological Location
Cytogenetic map Notes References 71A1-71A2     (Bodner, 1999.11.24)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\BobA for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) BobA-RA FBtr0075673  NM_080348   614   78
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein BobA-PA   FBpp0075426   8.9   78   9.21     NP_525087   AAF49695
Additional Polypeptide Data & Comments
Reported size (kDa)	78 (aa) (Lai et al., 2000)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141792   AE014296 AAF49695   AF208486 AAF33202   BT023600   CO178252   CO189476   CO283991   EC069318   EC195799   EC212704   EL876467   EL876587   EL876695   FF579353   FF579603
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	P83262 P83263
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0040487    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0040487 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of no expression detected. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0040487 expression level Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr    20187 embryo 06-08hr    12618 embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (8379) embryo 04-06hr  (20187) embryo 06-08hr  (12618) embryo 08-10hr  (7441) embryo 10-12hr  (1053) embryo 12-14hr  (302) embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr  107 embryo 20-22hr    57 embryo 22-24hr  (126) larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr  (8379) embryo 04-06hr  (20187) embryo 06-08hr  (12618) embryo 08-10hr  (7441) embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr  (20187) embryo 06-08hr  (12618) embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr    20187 embryo 06-08hr    12618 embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0040487 expression level Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr    20187 embryo 06-08hr    12618 embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (8379) embryo 04-06hr  (20187) embryo 06-08hr  (12618) embryo 08-10hr  (7441) embryo 10-12hr  (1053) embryo 12-14hr  (302) embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr  107 embryo 20-22hr    57 embryo 22-24hr  126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr  (8379) embryo 04-06hr  (20187) embryo 06-08hr  (12618) embryo 08-10hr  (7441) embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr  20187 embryo 06-08hr  12618 embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    8379 embryo 04-06hr    20187 embryo 06-08hr    12618 embryo 08-10hr    7441 embryo 10-12hr    1053 embryo 12-14hr    302 embryo 14-16hr    76 embryo 16-18hr    85 embryo 18-20hr    107 embryo 20-22hr    57 embryo 22-24hr    126 larva L1    0 larva L2    3 larva L3 12hr old    7 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    0 white prepupae new    4 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    15 pupae 3d postWPP    0 pupae 4d postWPP    8 adult male 01day    0 adult male 05day    4 adult male 30day    0 adult female 01day    13 adult female 05day    12 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0040487    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0040487 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0040487 expression level Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0040487 expression level Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    24.775 Larval Midgut    4.2 Larval Hindgut    3.5 Larval Malpighian Tubules    3.9 Larval Fat Body    7.6 Larval Salivary Gland    5.2 Larval Trachea    3.65 Larval Carcass    3.5 Adult Head    0.8 Adult Eye    2.4 Adult Brain    2.2 Adult Thoracic-Abdominal Ganglion    1.2 Adult Crop    5.9 Adult Midgut    2.4 Adult Hindgut    2.9 Adult Malpighian Tubules    2.6 Adult Fat Body    8.4 Adult Salivary Gland no informative data Adult Heart    3.2 Adult VirginFemale Spermatheca    4.7 Adult InseminatedFemale Spermatheca    3.4 Adult Ovary    0.9 Adult Testis    2.1 Adult Male Accessory Gland    7.7 Adult Carcass    7.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    0.8   NA  Eye    2.4   NA  Brain    2.2   24.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1.2   NA  Crop    5.9   4.2  Midgut    2.4   3.5  Hindgut    2.9   3.9  Malpighian Tubules    2.6   7.6  Fat Body    8.4   5.2  Salivary Gland    no informative data   NA  Heart    3.2   3.65  Trachea    NA   NA  VirginFemale Spermatheca    4.7   NA  InseminatedFemale Spermatheca    3.4   NA  Ovary    0.9   NA  Testis    2.1   NA  Male Accessory Gland    7.7   3.5  Carcass    7.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_31
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0040487 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12487-RA;CG13465-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4sca-537.4 BobAScer\UAS.cLa viable BobAf03024 Sterility Allele fertile BobAf03024 Other Phenotypes Allele visible, with Scer\GAL469B BobAScer\UAS.cLa visible, with Scer\GAL4Bx-MS1096 BobAScer\UAS.cLa visible, with Scer\GAL4sca-109-68 BobAScer\UAS.cLa visible, with Scer\GAL4sca-537.4 BobAScer\UAS.cLa Phenotype manifest in Allele external sensory organ precursor cell & dorsal mesothoracic disc, with Scer\GAL4sca-537.4 BobAScer\UAS.cLa macrochaeta, with Scer\GAL4sca-537.4 BobAScer\UAS.cLa microchaeta, with Scer\GAL4sca-537.4 BobAScer\UAS.cLa tormogen cell, with Scer\GAL4sca-109-68 BobAScer\UAS.cLa trichogen cell, with Scer\GAL4sca-109-68 BobAScer\UAS.cLa wing margin bristle, with Scer\GAL469B BobAScer\UAS.cLa wing margin bristle, with Scer\GAL4Bx-MS1096 BobAScer\UAS.cLa wing sensillum & wing vein, with Scer\GAL469B BobAScer\UAS.cLa wing sensillum & wing vein, with Scer\GAL4Bx-MS1096 BobAScer\UAS.cLa
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of BobA Class Mutagen Stocks Known lesion BobAf03024 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of BobA Class Mutagen Stocks Known lesion BobAScer\UAS.cLa in vitro construct - regulatory fusion 2 Yes BobAKK114961 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Brd-C1 (Chanet et al., 2009)
Duplicated in	Dp(3;2)Brd-C (Chanet et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13030} NA P{GD15234} NA P{GD17347} NA P{KK114961} NA P{UAS-BobA.L} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}BobAf03024 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell fate specification inferred from mutant phenotype (Lai et al., 2000) Notch signaling pathway inferred from mutant phenotype (Lai et al., 2000) sensory organ development inferred from mutant phenotype (Lai et al., 2000) sensory organ precursor cell fate determination inferred from mutant phenotype (Lai et al., 2000)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions BobA allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0040487
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs	Dyak\GE19996
Linkouts	OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0237322 FBgn0068461 FBgn0200949 FBgn0184319 FBgn0184318 FBgn0179383 FBgn0179385 FBgn0163578 FBgn0163558 FBgn0136693 FBgn0040809 FBgn0124404 FBgn0105964 FBgn0102169
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	26683 w*; P{UAS-BobA.L}2 P{UAS-BobA.L}7 18609 w1118; PBac{WH}BobAf03024 26684 w*; P{UAS-BobA.L}3 P{UAS-BobA.L}10
Harvard	- PBac{WH}BobAf03024
Genomic Clones ( 2 )
	BACR01H12 BACR22J22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP06720
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	75293 CK01.54D.F10 CK01.70D.H9 CK02476 Diego_25_G05 EK044355 EK056231 EK164960 IP06420 IP06520 IP06620 Parral_28_F05
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0040487 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 50281
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: BobA CG12487 (FlyBase, 1996-)
Additional comments	"CG12487" may correspond to "BobA" or "BobB" but does not correspond to "BobC". "BobA" may correspond to "CG12487" but does not correspond to "CG13465". (Whitfield, 2000.10.30) Source for merge of BobA CG12487 was sequence comparison (date:041206). (FlyBase, 1996-) In FBrf0123097, 3 tandemly arranged loci, which encode nearly identical "Bob" proteins have been detected in genomic clones of the 71A1-71A2 region. These loci are "BobA", "BobB" and "BobC". In the sequenced D.melanogaster genome, only two copies of "Bob" genes are present in the corresponding genomic region. These two genes are represented by the annotations "CG12487" and "CG13465". The annotation "CG12487" corresponds to "BobA". It is not possible to tell whether the "CG13465 annotation corresponds to "BobB" or "BobC". (FlyBase, 1996-)
Other Comments
	Overexpression of BobA antagonises the N pathway in multiple cell fate decisions during adult sensory organ development. (Lai et al., 2000) Brd family overexpression phenotypes resemble N loss of function phenotypes. (Bodner et al., 1999) Overexpression of Bob results in both bristle tufting and balding phenotypes. (Lai et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080348 RefSeq (Proteins) • NP_525087 DDBJ / EMBL / GenBank • AAF33202 AAF49695 AF208486 Entrez Gene - A searchable database of RefSeq genes. • 50281 UniProtKB/TrEMBL • P83262 P83263
Other Crossreferences
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0040487 DRSC - Results from RNAi screens. • FBgn0040487 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0040487 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12487-RA;CG13465-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0040487 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 50281 modMine - Data generated by the modENCODE project. • FBgn0040487 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0237322 FBgn0068461 FBgn0200949 FBgn0184319 FBgn0184318 FBgn0179383 FBgn0179385 FBgn0163578 FBgn0163558 FBgn0136693 FBgn0040809 FBgn0124404 FBgn0105964 FBgn0102169
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	Bob (Lai, 2002, Lai et al., 1999, Bodner et al., 1999, Fontana and Posakony, 2009, Osada and Innan, 2008) bobA (Ayres et al., 2008) Bob-A (Lai et al., 2005) BobA (Reiter et al., 2007, Parks and Muskavitch, 2009.2.2, Chanet et al., 2009, Egger et al., 2010) CG12487 (Ayres et al., 2008, Whitfield, 2000.10.30, Keleman et al., 2009.8.5)
Name Synonym	Brother of Bearded (Osada and Innan, 2008) Brother of Bearded A (Bodner, 1999.11.24, Lai et al., 2000)
Secondary FlyBase IDs
	FBgn0026561 FBgn0040808
References ( 20 )
Research paper	Egger et al., 2010, Development 137(18): 2981--2987 Notch regulates the switch from symmetric to asymmetric neural stem cell division in the Drosophila optic lobe. [FBrf0211572] Chanet et al., 2009, Genetics 182(4): 1101--1108 Genome engineering-based analysis of bearded family genes reveals both functional redundancy and a nonessential function in lateral inhibition in Drosophila. [FBrf0208667] Fontana and Posakony, 2009, Dev. Biol. 333(2): 373--385 Both inhibition and activation of Notch signaling rely on a conserved Neuralized-binding motif in Bearded proteins and the Notch ligand Delta. [FBrf0208547] Ayres et al., 2008, Genetics 178(3): 1807--1815 Identification of Drosophila mutants altering defense of and endurance to Listeria monocytogenes infection. [FBrf0205079] Osada and Innan, 2008, PLoS Genet. 4(12): e1000305 Duplication and gene conversion in the Drosophila melanogaster genome. [FBrf0206545] Reiter et al., 2007, Fly 1(3): 164--171 Accentuate the negative: proteome comparisons using the negative proteome database. [FBrf0200136] Lai et al., 2005, Genes Dev. 19(9): 1067--1080 Pervasive regulation of Drosophila Notch target genes by GY-box-, Brd-box-, and K-box-class microRNAs. [FBrf0187594] Lai, 2002, Nature Genetics 30(4): 363--364 Micro RNAs are complementary to 3' UTR sequence motifs that mediate negative post-transcriptional regulation. [FBrf0151441] Lai et al., 2000, Development 127(2): 291--306 Antagonism of Notch signaling activity by members of a novel protein family encoded by the Bearded and Enhancer of split gene complexes. [FBrf0123097] Lai et al., 2000, Development 127(16): 3441--3455 The Enhancer of split Complex of Drosophila includes four Notch-regulated members of the Bearded gene family. [FBrf0128540]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Parks and Muskavitch, 2009.2.2, P{UAS-BobA.L} insertions. P{UAS-BobA.L} insertions. [FBrf0206631] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] Posakony, 2000.2.8, Bob genes. Bob genes. [FBrf0125100] Whitfield, 2000.10.30, FlyBase error report on Mon Oct 30 16:31:09 2000. FlyBase error report on Mon Oct 30 16:31:09 2000. [FBrf0132135]
Abstract	Bodner et al., 1999, A. Dros. Res. Conf. 40: 509A A new gene family encoding potential antagonists of Notch signalling in the eye. [FBrf0106240] Lai et al., 1999, A. Dros. Res. Conf. 40: 73 A novel family of five Notch-pathway regulators encoded by members of the Enhancer of split and Bearded gene complexes. [FBrf0106809]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
DNA/RNA sequence record	Bodner, 1999.11.24, GenBank/EMBL/DDBJ: AF208486 Drosophila melanogaster Brother of Bearded protein A (BobA), Brother of Bearded protein B (BobB), and Brother of Bearded protein C (BobC) genes, complete cds; and putative microtubule-associated protein gene, partial cds. [FBrf0125532]