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Gene Dmel\Buffy

General Information
Symbol	Dmel\Buffy	Species	D. melanogaster
Name	Buffy	Annotation symbol	CG8238
Feature type	protein_coding_gene	FlyBase ID	FBgn0040491
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	Buffy/dBorg-2, dBorg-2, Buffy/Borg2
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	48C1-48C1	Sequence location	2R:7,566,978..7,576,264 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Buffy is referred to in FlyBase by the symbol Dmel\Buffy (CG8238, FBgn0040491). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: anti-apoptosis; positive regulation of programmed cell death. 11 alleles are reported. The phenotypes of these alleles are annotated with: glial cell; presumptive embryonic/larval nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: Apoptosis regulator, Bcl-2, BH; Apoptosis regulator, Bcl2-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult midgut, adult hindgut, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1623425_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Buffy. Gene sequence location is 2R:7566978..7576264.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Sequence features HFA05957 BKN33202
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 48C1-48C1   Limits computationally determined from genome sequence between P{lacW}oho48Ak06524 and P{EP}walEP825&P{PZ}wal02516
Experimentally Determined Cytological Location
Cytogenetic map Notes References 48A-48E     (Colussi, 2000.2.22)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Buffy for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Buffy-RA FBtr0088077  NM_078978   1259   299
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Buffy-PA   FBpp0087182   33.4   299   10.28     NP_523702   AAF58628
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Apoptosis regulator, Bcl-2, BH (IPR000712) Apoptosis regulator, Bcl2-like (IPR002475)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58628   AC007473   AC016794   AF237864 AAF44120   AY075219 AAL68086   BF501258   BI369877   BP541299   BP559585   BP559586   CL844429   CO306286   CO319045   CO321384   CO327088   EC088726   EC223481   EC227390   EC254474
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8T8Y5 Q9NGX3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   organism | ubiquitous   (Brachmann et al., 2000) embryonic stage 12 -- 13   mesoderm   (Brachmann et al., 2000) northern blot Stage Tissue/Position (including subcellular localization) Reference pupal stage   salivary gland   (Lee et al., 2003) RT-PCR Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   organism   (Brachmann et al., 2000) adult stage | male   head   (Brachmann et al., 2000) abdomen & thorax   (Brachmann et al., 2000) adult stage | female   head   (Brachmann et al., 2000) abdomen & thorax   (Brachmann et al., 2000)
Additional Descriptive Data
	The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue. (Lee et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0040491    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0040491 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0040491 expression level Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr  (234) embryo 12-14hr  (205) embryo 14-16hr  (275) embryo 16-18hr  (496) embryo 18-20hr  (958) embryo 20-22hr  (509) embryo 22-24hr  (582) larva L1  (297) larva L2  (230) larva L3 12hr old  (374) larva L3 puffstage 1-2  (191) larva L3 puffstage 3-6  (324) larva L3 puffstage 7-9  (788) white prepupae new  (870) white prepupae 12hr  (1669) white prepupae 24hr  (291) pupae 2d postWPP  (810) pupae 3d postWPP  (981) pupae 4d postWPP  (1368) adult male 01day  (1008) adult male 05day  (1286) adult male 30day  (1213) adult female 01day  (464) adult female 05day  (314) adult female 30day  (273) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr  (1669) white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0040491 expression level Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr  (234) embryo 12-14hr  (205) embryo 14-16hr  (275) embryo 16-18hr  (496) embryo 18-20hr  (958) embryo 20-22hr  (509) embryo 22-24hr  (582) larva L1  (297) larva L2  (230) larva L3 12hr old  (374) larva L3 puffstage 1-2  (191) larva L3 puffstage 3-6  (324) larva L3 puffstage 7-9  (788) white prepupae new  (870) white prepupae 12hr  (1669) white prepupae 24hr  (291) pupae 2d postWPP  (810) pupae 3d postWPP  (981) pupae 4d postWPP  (1368) adult male 01day  (1008) adult male 05day  (1286) adult male 30day  (1213) adult female 01day  (464) adult female 05day  (314) adult female 30day  (273) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr  1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    6 embryo 02-04hr    37 embryo 04-06hr    33 embryo 06-08hr    45 embryo 08-10hr    96 embryo 10-12hr    234 embryo 12-14hr    205 embryo 14-16hr    275 embryo 16-18hr    496 embryo 18-20hr    958 embryo 20-22hr    509 embryo 22-24hr    582 larva L1    297 larva L2    230 larva L3 12hr old    374 larva L3 puffstage 1-2    191 larva L3 puffstage 3-6    324 larva L3 puffstage 7-9    788 white prepupae new    870 white prepupae 12hr    1669 white prepupae 24hr    291 pupae 2d postWPP    810 pupae 3d postWPP    981 pupae 4d postWPP    1368 adult male 01day    1008 adult male 05day    1286 adult male 30day    1213 adult female 01day    464 adult female 05day    314 adult female 30day    273 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0040491    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0040491 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult salivary gland, larval trachea. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult midgut, adult hindgut, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0040491 expression level Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop    1578.8 Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland    2127.4 Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea  (563.15) Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop  (1578.8) Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland  (2127.4) Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop  (1578.8) Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland  (2127.4) Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop    1578.8 Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland    2127.4 Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0040491 expression level Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop    1578.8 Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland    2127.4 Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea  563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop  (1578.8) Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland  (2127.4) Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop  1578.8 Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland  (2127.4) Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    20.625 Larval Midgut    32.9 Larval Hindgut    66.5 Larval Malpighian Tubules    53.2 Larval Fat Body    8.6 Larval Salivary Gland    37.1 Larval Trachea    563.15 Larval Carcass    21.225 Adult Head    119.6 Adult Eye    300.225 Adult Brain    13 Adult Thoracic-Abdominal Ganglion    23.9 Adult Crop    1578.8 Adult Midgut    122 Adult Hindgut    291 Adult Malpighian Tubules    71.3 Adult Fat Body    22.2 Adult Salivary Gland    2127.4 Adult Heart    79.6 Adult VirginFemale Spermatheca    60.9 Adult InseminatedFemale Spermatheca    74.5 Adult Ovary    21.9 Adult Testis    49.4 Adult Male Accessory Gland    413.8 Adult Carcass    41.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    119.6   NA  Eye    300.225   NA  Brain    13   20.625  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    23.9   NA  Crop    1578.8   32.9  Midgut    122   66.5  Hindgut    291   53.2  Malpighian Tubules    71.3   8.6  Fat Body    22.2   37.1  Salivary Gland    2127.4   NA  Heart    79.6   563.15  Trachea    NA   NA  VirginFemale Spermatheca    60.9   NA  InseminatedFemale Spermatheca    74.5   NA  Ovary    21.9   NA  Testis    49.4   NA  Male Accessory Gland    413.8   21.225  Carcass    41.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_18
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0040491 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8238-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | partially, with Scer\GAL4en-e16E BuffyScer\UAS.cQa viable BuffyEY11259 BuffyH37 Sterility Allele fertile BuffyEY11259 BuffyH37 Other Phenotypes Allele decreased cell death BuffyH37 decreased cell death, with Scer\GAL4en-e16E BuffyScer\UAS.cQa decreased cell death | conditional, with Scer\GAL4en-e16E BuffyScer\UAS.cQa BuffyΔN.Scer\UAS.T:Zzzz\FLAG increased cell death BuffydsRNA.cQa mitotic cell cycle defective, with Scer\GAL4en-e16E BuffyScer\UAS.cQa short lived, with Scer\GAL4elav-C155 BuffyScer\UAS.cQa Phenotype manifest in Allele glial cell BuffyH37 presumptive embryonic/larval nervous system BuffydsRNA.cQa
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Buffy Class Mutagen Stocks Known lesion BuffyH37 loss of function allele Delta2-3 3 Yes BuffyEY11259 P-element activity 1 -- BuffyMB04892 1 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Buffy Class Mutagen Stocks Known lesion BuffydsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BuffyHMS01484 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BuffyJF03287 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes BuffyScer\UAS.cSa in vitro construct - regulatory fusion 1 Yes BuffydsRNA.cQa in vitro construct - RNAi 0 Yes BuffyGD12518 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes BuffyScer\UAS.cQa in vitro construct - regulatory fusion 0 Yes BuffyΔN.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12518} NA P{TRiP.HMS01484} NA P{TRiP.JF03287} NA P{UAS-Buffy.Q} NA P{UAS-Buffy.RNAi} NA P{UAS-Buffy.S} NA P{UAS-Buffy.ΔN.FLAG} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}BuffyEY11259 NA insertion of enhancer trap binary system Mi{ET1}CG13196MB04892 No
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
anti-apoptosis inferred from genetic interaction with grim (Quinn et al., 2003) inferred from genetic interaction with rpr (Quinn et al., 2003) inferred from genetic interaction with th (Quinn et al., 2003) inferred from genetic interaction with W (Quinn et al., 2003) inferred from mutant phenotype (Quinn et al., 2003) positive regulation of programmed cell death inferred from genetic interaction with grim (Wu et al., 2010) inferred from mutant phenotype (Wu et al., 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
apoptotic process inferred from sequence or structural similarity with human BCL2 (Brachmann et al., 2000) inferred from sequence or structural similarity with mouse Bcl2l (FlyBase, 1992-) non-traceable author statement (Lawen, 2003) induction of apoptosis inferred from sequence or structural similarity with NCBI_gi:5802578 (Vernooy et al., 2000) programmed cell death traceable author statement (Richardson and Kumar, 2002) regulation of apoptosis inferred from electronic annotation with InterPro:IPR000712, InterPro:IPR002475 (FlyBase Curators et al., 2004-) traceable author statement (Richardson and Kumar, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Buffy allele Gene References BuffyScer\UAS.cQa th (Quinn et al., 2003) unspecified Pink1 (Park et al., 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62038 DroID - A comprehensive database of gene and protein interactions. • FBgn0040491 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36251
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11452 Dere\GG20214 Dgri\GH21024 Dmoj\GI18624 Dper\GL10080 Dpse\GA20921 Dsec\GM21300 Dsim\GD10809 Dvir\GJ21633 Dwil\GK17878 Dyak\GE12374
Curated drosophilid orthologs	Dpse\GA20921
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001521-PA Aedes aegypti (mosquito) XP_395083.3 Apis mellifera (honey bee) XP_001948797.1 Acyrthosiphon pisum (pea aphid) ENSCINP00000019812 Ciona intestinalis (sea squirt) CPIJ002201 Culex pipens (mosquito) ENSCSAVP00000012272 Ciona savignyi (sedentary tunicate) 148538 Capitella spl (polychaete worm) ENSDARP00000068408 Danio rerio (zebra fish) ENSDARP00000090569 Danio rerio (zebra fish) ENSGACP00000006084 Gymnopilus aculeatus (mushroom) ENSGALP00000009257 Gallus gallus (chicken) 183772 Hypsipyla robusta (cedar shoot borer) ENSMODP00000020066 Monodelphis domestica (gray opossum) 139439 Nematostella vectensis (starlet anemone) XP_001604280.1 Nasonia vitripennis (wasp) PHUM005550-PA Pediculus humanus (human body louse) 63759 Trichoplax adhaerens (metazoa) XP_966641.2 Tribolium castaneum (red flour beetle) ENSTNIP00000005841 Tetraodon nigroviridis (green spotted pufferfish) ENSTNIP00000018321 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000018728 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000030263 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13205 ISCW019088 ISCW008937 FBgn0230120 FBgn0219877 FBgn0208752 FBgn0182572 FBgn0176181 FBgn0080910 FBgn0147690 FBgn0141363 FBgn0128486 FBgn0112405 FBgn0088492 CPIJ002201 ACYPI005643 GB18455 AAEL001521 PHUM582920 GLEAN_13830
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	32059 w*; P{UAS-Buffy.S}E1 32060 w*; P{UAS-Buffy.RNAi}3 27340 w1118; BuffyH37 29608 y1 v1; P{TRiP.JF03287}attP2 27338 w1118; debclE26 BuffyH37 27339 w1118; debclW105 BuffyH37 20280 y1 w67c23; P{EPgy2}BuffyEY11259 35742 y1 sc* v1; P{TRiP.HMS01484}attP2 24309 w1118; Mi{ET1}CG13196MB04892 BuffyMB04892
Genomic Clones ( 2 )
	BACR35F01 BACR38D12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 12 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT16536
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE56209
Other clones	62146 144304 174760 214481.14 EK185560 EK250714 EK276057 EP05733 FGB001E06 FGM321G05
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0040491 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36251
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Buffy CG8238 (FlyBase, 1996-) Source for identity of: dBorg-2 Buffy (Brachmann et al., 2000)
Source for database merge of
Additional comments	Source for identity of Buffy CG8238 was sequence comparison (date:000327). (FlyBase, 1996-)
Other Comments
	Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB. (Liu and Lehmann, 2008) Buffy is not essential for normal development. (Sevrioukov et al., 2007) The Buffy product inactivates the debcl protein. (Senoo-Matsuda et al., 2005) dsRNA made from templates generated with primers directed against this gene causes a phenotype in S2 cells. Cell death induction by treatment with proteasome inhibitors, rotenone, or 3-NP is suppressed, while cell death induction by tunicamycin is not affected. (Senoo-Matsuda et al., 2005) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Buffy is required for cell survival and can prevent developmental and irradiation induced cell death. (Quinn et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078978 RefSeq (Proteins) • NP_523702 DDBJ / EMBL / GenBank • AAF44120 AAF58628 AAL68086 AC007473 AC016794 AF237864 AY075219 BF501258 CL844429 Entrez Gene - A searchable database of RefSeq genes. • 36251 UniProtKB/TrEMBL • Q8T8Y5 Q9NGX3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Apoptosis regulator, Bcl-2, BH (IPR000712) Apoptosis regulator, Bcl2-like (IPR002475)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62038 DroID - A comprehensive database of gene and protein interactions. • FBgn0040491 DRSC - Results from RNAi screens. • FBgn0040491 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0040491 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8238-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0040491 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36251 InParanoid A subset of ortholog calls from InParanoid. • AAEL001521-PA Aedes aegypti (mosquito) XP_395083.3 Apis mellifera (honey bee) XP_001948797.1 Acyrthosiphon pisum (pea aphid) ENSCINP00000019812 Ciona intestinalis (sea squirt) CPIJ002201 Culex pipens (mosquito) ENSCSAVP00000012272 Ciona savignyi (sedentary tunicate) 148538 Capitella spl (polychaete worm) ENSDARP00000068408 Danio rerio (zebra fish) ENSDARP00000090569 Danio rerio (zebra fish) ENSGACP00000006084 Gymnopilus aculeatus (mushroom) ENSGALP00000009257 Gallus gallus (chicken) 183772 Hypsipyla robusta (cedar shoot borer) ENSMODP00000020066 Monodelphis domestica (gray opossum) 139439 Nematostella vectensis (starlet anemone) XP_001604280.1 Nasonia vitripennis (wasp) PHUM005550-PA Pediculus humanus (human body louse) 63759 Trichoplax adhaerens (metazoa) XP_966641.2 Tribolium castaneum (red flour beetle) ENSTNIP00000005841 Tetraodon nigroviridis (green spotted pufferfish) ENSTNIP00000018321 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000018728 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000030263 Takifugu rubripes (pufferfish Takifugu) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/buffy1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36251 modMine - Data generated by the modENCODE project. • FBgn0040491 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13205 ISCW019088 ISCW008937 FBgn0230120 FBgn0219877 FBgn0208752 FBgn0182572 FBgn0176181 FBgn0080910 FBgn0147690 FBgn0141363 FBgn0128486 FBgn0112405 FBgn0088492 CPIJ002201 ACYPI005643 GB18455 AAEL001521 PHUM582920 GLEAN_13830
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	Bcl-2-48AE   buffy (Ortega et al., 2005, Monserrate et al., 2005, Gorski et al., 2003, Vernooy et al., 2000, Senoo-Matsuda et al., 2005, Sevrioukov et al., 2007, Tsubouchi et al., 2009, Wu et al., 2010, Terhzaz et al., 2010) Buffy (Park et al., 2006, Kuranaga and Miura, 2007, Liu and Lehmann, 2008, Zeitouni et al., 2007, Cao et al., 2007, Hou et al., 2008, Martin et al., 2007, Kadam et al., 2009, Mast et al., 2008, Wu et al., 2009, Lam et al., 2010) BUFFY (Doumanis et al., 2007) Buffy/Borg2 (Purves et al., 2005, Burr et al., 2005) Buffy/dBorg-2 (Igaki and Miura, 2004, Danial and Korsmeyer, 2004, Richardson and Kumar, 2002, Igaki et al., 2002, Galindo and Abrams, 2007) Buffy/DBorg-2 (Vernooy et al., 2000) CG8238 (Lee et al., 2003, Perkins et al., 2009, Ni et al., 2011.7.19) dBorg-2 (Gorski and Marra, 2002, Vernooy et al., 2000, Brachmann et al., 2000, Kornbluth and White, 2005) dBorg2 (Sevrioukov et al., 2007) dBORG-2/BUFFY (Meier et al., 2000) dbuffy (Monserrate et al., 2007) Dbx (Zhang et al., 2000) Drob2 (Tsubouchi et al., 2009)
Name Synonym	48A-E Bcl-2-like (Colussi et al., 2000) Buffy (Sevrioukov et al., 2007, Kornbluth and White, 2005, Zeitouni et al., 2007, Martin et al., 2007, Wu et al., 2009) BUFFY (Doumanis et al., 2007) buffy (Brachmann, 2010.8.2)
Secondary FlyBase IDs
	FBgn0033646
References ( 62 )
Generate a list of	All references relating to this gene ( 62 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 4 ) Review  ( 12 ) Personal communication to FlyBase  ( 5 ) Abstract  ( 6 ) FlyBase analysis  ( 2 ) Letter  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 4 )
	Tanner et al., 2011, Development 138(2): 327--338 Bcl-2 proteins and autophagy regulate mitochondrial dynamics during programmed cell death in the Drosophila ovary. [FBrf0212552] Lam et al., 2010, Cell Death Dis. 1(8): e66 Drosophila happyhour modulates JNK-dependent apoptosis. [FBrf0213175] Terhzaz et al., 2010, Cell. Signal. 22(5): 737--748 Cell-specific inositol 1,4,5 trisphosphate 3-kinase mediates epithelial cell apoptosis in response to oxidative stress in Drosophila. [FBrf0210037] Wu et al., 2010, Mech. Dev. 127(9-12): 407--417 grim promotes programmed cell death of Drosophila microchaete glial cells. [FBrf0212047] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...