FlyBase Gene Report: Dmel\Alk

General Information

Symbol

Dmel\Alk

Species

D. melanogaster

Name

Anaplastic lymphoma kinase

Annotation Symbol

CG8250

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040505

Gene Model Status

Stock Availability

7 publicly available

Enzyme Name (EC)

Receptor protein-tyrosine kinase (2.7.10.1)

Gene Summary

Anaplastic lymphoma kinase (Alk) encodes a protein that employs Ras/ERK and PI3K signalling pathways to function in multiple contexts including embryonic visceral muscle development, growth and organ size regulation, retinal axon targeting, modulation of neuromuscular transmission as well as sleep regulation and olfactory learning. Its ligand is the secreted protein encoded by jeb. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DAlk

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:16,625,293..16,640,011 [-]

Recombination map

2-80

RefSeq locus

NT_033778 REGION:16625293..16640011

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (23 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:cnk; FB:FBgn0286070

(Wolfstetter et al., 2017)

enables transmembrane receptor protein tyrosine kinase activity

inferred from direct assay

(Yang et al., 2007, Lee et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002011, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables transmembrane receptor protein tyrosine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN001230349

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (15 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in axon guidance

inferred from mutant phenotype

(Bazigou et al., 2007)

involved_in compound eye photoreceptor development

inferred from mutant phenotype

(Bazigou et al., 2007)

involved_in digestive tract development

inferred from mutant phenotype

(Loren et al., 2003)

involved_in mesoderm development

inferred from mutant phenotype

(Bazigou et al., 2007)

involved_in negative regulation of cell size

inferred from mutant phenotype

(Gouzi et al., 2011)

involved_in negative regulation of neuromuscular synaptic transmission

inferred from mutant phenotype

(Rohrbough et al., 2013)

involved_in positive regulation of ERK1 and ERK2 cascade

inferred from mutant phenotype

(Gouzi et al., 2011)

involved_in positive regulation of MAPK cascade

inferred from mutant phenotype

(Rohrbough et al., 2013)

involved_in regulation of growth

inferred from mutant phenotype

(Gouzi et al., 2011)

involved_in regulation of olfactory learning

inferred from mutant phenotype

(Gouzi et al., 2011)

involved_in transmembrane receptor protein tyrosine kinase signaling pathway

inferred from direct assay

(Yang et al., 2007)

involved_in visceral muscle development

inferred from mutant phenotype

(Englund et al., 2003, Lee et al., 2003)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in positive regulation of kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN001230349

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein phosphorylation

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245, InterPro:IPR002011, InterPro:IPR008266, InterPro:IPR020635

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

involved_in regulation of neuron differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000699407

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in transmembrane receptor protein tyrosine kinase signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001230349

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in subsynaptic reticulum

inferred from direct assay

(Rohrbough et al., 2013)

located_in type Ib terminal bouton

inferred from direct assay

(Rohrbough et al., 2013)

located_in type Is terminal bouton

inferred from direct assay

(Rohrbough et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN001230349

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of receptor complex

inferred from biological aspect of ancestor with PANTHER:PTN001230349

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

RECEPTOR TYROSINE KINASES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.1)

Predictions / Assertions

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (2.7.10.1)

Summaries

Gene Snapshot

Anaplastic lymphoma kinase (Alk) encodes a protein that employs Ras/ERK and PI3K signalling pathways to function in multiple contexts including embryonic visceral muscle development, growth and organ size regulation, retinal axon targeting, modulation of neuromuscular transmission as well as sleep regulation and olfactory learning. Its ligand is the secreted protein encoded by jeb. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

RECEPTOR TYROSINE KINASES -: Receptor tyrosine kinases (RTK) are single-pass transmembrane receptors expressed on the plasma membrane. Upon the binding of an extracellular signalling molecule (e.g. growth factors, hormones), RTKs dimerize leading to the activation of the intracellular tyrosine kinase domain and intermolecular phosphorylation. The phosphotyrosines function as specific sites for the assembly, phosphorylation and activation of downstream signaling molecules. (Adapted from PMID:20602996).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Alk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KJ08)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0087118

8655

1701

FBtr0331926

9402

1701

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0086264

185.6

1701

6.55

FBpp0304259

185.6

1701

6.55

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1701 aa isoforms: Alk-PA, Alk-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Alk using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Alk_1
Alk_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

fusion competent cell of visceral mesoderm

• plasma membrane

(Stute et al., 2004)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

visceral mesoderm | restricted

• plasma membrane

(Stute et al., 2004)

myoblast | subset

• plasma membrane

(Stute et al., 2004)

muscle founder cell of visceral mesoderm

• plasma membrane

(Stute et al., 2004)

embryonic stage 17

embryonic/larval neuromuscular junction

Comment: type Ib boutons

(Rohrbough and Broadie, 2010)

Comment: synapse

(Rohrbough and Broadie, 2010)

third instar larval stage

embryonic/larval brain | restricted

(Gouzi et al., 2011)

larval ventral nerve cord

(Gouzi et al., 2011)

mushroom body calyx

(Gouzi et al., 2011)

lamina plexus | faint

(Bazigou et al., 2007)

medulla anlage | intense

(Bazigou et al., 2007)

wandering third instar larval stage

embryonic/larval neuromuscular junction

• postsynaptic density

(Rohrbough et al., 2013)

pupal stage -- adult stage

(Bazigou et al., 2007)

optic cartridge

(Bazigou et al., 2007)

(Bazigou et al., 2007)

adult brain | restricted

(Gouzi et al., 2011)

calyx of adult mushroom body

(Gouzi et al., 2011)

adult optic lobe

(Gouzi et al., 2011)

protocerebral bridge

(Gouzi et al., 2011)

gnathal ganglion

(Gouzi et al., 2011)

adult antennal lobe

(Gouzi et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Alk protein is expressed throughout the third instar larval and adult brain. In the adult, labelling is stronger in the mushroom body calyx (dendrites of Kenyon cells), protocerebral bridge, antennal lobe, subesophageal ganglion and optic lobe. Alk protein is not detected in the mushroom body lobes (axons of Kenyon cells). The expression of Alk protein is similar but more widespread than labelling with Scer\GAL4^Alk.38.

(Gouzi et al., 2011)

Alk is concentrated postsynaptically at larval glutamergic neuromusclular junction synapses. It is strongly expressed in type Ib boutons and is more weakly localized at type Is boutons. Alk superimposed extensively with the anti-HRP labeled presynaptic membrane, but had a much broader domain and was localized to the surrounding postsynaptic muscle. In mature embryos, Alk is present in developing CNS synapses.

(Rohrbough and Broadie, 2010)

In third instar larvae, Alk protein is expressed at low levels in the lamina plexus, and at high levels in the medulla neuropil; expression is absent from photoreceptor axons and repo-positive glial cells. At pupal timepoints 24, 40, and 50 hours APF, and at adult stages, Alk protein expresson increases in optic cartridges (lamina cartridges) and remains strong in the medulla and lobula neuropils. The expression pattern in the developing visual system is complementay to that of jeb.

(Bazigou et al., 2007)

Expression of Alk protein is observed in both muscle founder cells and in fusion-competent myoblasts in the visceral mesoderm, and is localized to the cell membrane.

(Stute et al., 2004)

Marker for

visceral mesoderm

(Eriksson et al., 2010)

Subcellular Localization

CV Term

Evidence

References

located_in subsynaptic reticulum

inferred from direct assay

(Rohrbough et al., 2013)

located_in type Ib terminal bouton

inferred from direct assay

(Rohrbough et al., 2013)

located_in type Is terminal bouton

inferred from direct assay

(Rohrbough et al., 2013)

Expression Deduced from Reporters

Reporter: P{Alk-GAL4.6.5}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

visceral mesoderm

(Englund et al., 2003)

embryonic stage 11 -- 12

visceral mesoderm

(Loren et al., 2001)

Reporter: P{Alk-GAL4.38}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval brain | restricted

(Gouzi et al., 2011)

larval ventral nerve cord

(Gouzi et al., 2011)

mushroom body calyx

(Gouzi et al., 2011)

adult brain | restricted

(Gouzi et al., 2011)

calyx of adult mushroom body

(Gouzi et al., 2011)

lobe system of mushroom body

(Gouzi et al., 2011)

protocerebral bridge

(Gouzi et al., 2011)

gnathal ganglion

(Gouzi et al., 2011)

adult antennal lobe

(Gouzi et al., 2011)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Alk in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Alk

View exonic expression by tissue for Dmel\Alk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53425/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040505

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040505

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040505

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8250

Flygut - An atlas of the Drosophila adult midgut

FBgn0040505

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 25 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Alk

Transgenic constructs containing regulatory region of Alk

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

(Loren et al., 2003)

(Stute et al., 2004)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | embryonic stage, with Scer\GAL4^Act5C.PI

Alk^UAS.cLa

lethal | embryonic stage | recessive

Alk¹

lethal | first instar larval stage | recessive

Alk¹

lethal | heat sensitive

Alk^ts

lethal | heat sensitive (with Alk¹)

Alk^ts

lethal | heat sensitive (with Alk^ts)

Alk¹

lethal | somatic clone, with Scer\GAL4^Act5C.PI

Alk^UAS.cLa

lethal - all die before end of larval stage, with Scer\GAL4^Alk.38

Alk^UAS.cLa

partially lethal, with Scer\GAL4^phm.PO

Alk^{EC.UAS.Tag:MYC}

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Alk^GD42

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Alk^GD42

viable, with Scer\GAL4^elav-C155

Other Phenotypes

Allele

abnormal cell migration | embryonic stage 13

Alk¹

abnormal developmental rate, with Scer\GAL4^GS.spok

Alk^{act.UAS.Tag:HA}

abnormal developmental rate, with Scer\GAL4^phm.PO

abnormal developmental rate, with Scer\GAL4^spok.1.45

Alk^{act.UAS.Tag:HA}

abnormal learning | adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B

abnormal learning | adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

abnormal learning | adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B

abnormal locomotor behavior | adult stage

Alk^MB06458

abnormal locomotor rhythm

Alk¹

abnormal locomotor rhythm, with Scer\GAL4^elav-C155

Alk^UAS.cRa

abnormal locomotor rhythm, with Scer\GAL4^how-24B

Alk^UAS.cRa

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR9B08

Alk^{dsRNA.UAS.cUa}

abnormal neuroanatomy | somatic clone

abnormal neuroanatomy | somatic clone, with Scer\GAL4^Act5C.PI

Alk^{EC.UAS.Tag:MYC}

abnormal neurophysiology

Alk¹

abnormal neurophysiology | third instar larval stage

Alk^ts

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

Alk^{dsRNA.WIZ.UAS}

abnormal neurophysiology | third instar larval stage (with Alk^f01491)

Alk^ts

abnormal neurophysiology | third instar larval stage (with Alk^ts)

Alk^f01491

abnormal size | pupal stage, with Scer\GAL4^386Y

abnormal size | pupal stage, with Scer\GAL4^Alk.38

abnormal size | pupal stage, with Scer\GAL4^elav-C155

abnormal size | pupal stage, with Scer\GAL4^Ras64B.PW

abnormal sleep, with Scer\GAL4^007Y

Alk^GD42

abnormal sleep, with Scer\GAL4^30Y

Alk^GD42

abnormal sleep, with Scer\GAL4^30Y, Scer\GAL80^Mef2.mb247

Alk^GD42

abnormal sleep, with Scer\GAL4^121Y

Alk^GD42

abnormal sleep, with Scer\GAL4^238Y

Alk^GD42

abnormal sleep, with Scer\GAL4^386Y

Alk^GD42

abnormal sleep, with Scer\GAL4¹⁴⁷¹

Alk^GD42

abnormal sleep, with Scer\GAL4^c309

Alk^GD42

abnormal sleep, with Scer\GAL4^c309, Scer\GAL80^Mef2.mb247

Alk^GD42

abnormal sleep, with Scer\GAL4^c320

Alk^GD42

abnormal sleep, with Scer\GAL4^c453

Alk^GD42

abnormal sleep, with Scer\GAL4^ChAT.7.4

Alk^GD42

abnormal sleep, with Scer\GAL4^elav.PU

Alk^GD42

abnormal sleep, with Scer\GAL4^ey-OK107

Alk^GD42

abnormal sleep, with Scer\GAL4^H24

Alk^GD42

abnormal sleep, with Scer\GAL4^MJ63

Alk^GD42

abnormal sleep, with Scer\GAL4^NP1131

Alk^GD42

abnormal sleep, with Scer\GAL4^nSyb.PS

Alk^GD42

abnormal sleep (with Alk¹)

Alk^ts

abnormal sleep (with Alk^ts)

Alk¹

abnormal sleep (with Df(2R)Exel7144)

Alk^ts

abnormal sleep | :heat sensitive | conditional

Alk^ts

abnormal sleep | heat sensitive (with Alk¹)

Alk^ts

abnormal sleep | heat sensitive (with Alk^ts)

Alk¹

abnormal sleep | heat sensitive (with Df(2R)Exel7144)

Alk^ts

abnormal sleep | recessive | heat sensitive

Alk^ts

decreased body size | P-stage, with Scer\GAL4^spok.1.45

Alk^{act.UAS.Tag:HA}

decreased cell size | adult stage, with Scer\GAL4^Ras64B.PW

increased body size | P-stage, with Scer\GAL4^phm.PO

Alk^JF02668

increased cell death | embryonic stage 13

Alk¹

increased cell number | larval stage, with Scer\GAL4^He.PZ

increased cell number | larval stage, with Scer\GAL4^Hml.PG

Alk^{act.UAS.Tag:HA}

increased cell size | adult stage, with Scer\GAL4^Ras64B.PW

increased size | larval stage, with Scer\GAL4^spok.1.45

Alk^{act.UAS.Tag:HA}

short lived, with Scer\GAL4^elav.PU

Alk^UAS.cLa

some die during larval stage, with Scer\GAL4^Alk.38

Alk^UAS.cLa

some die during pupal stage, with Scer\GAL4^pnr-MD237

Alk^GD42

visible | adult stage, with Scer\GAL4^sev.PU

Alk^UAS.cLa

Phenotype manifest in

Allele

axon & photoreceptor cell R8 | somatic clone

axon & photoreceptor cell R8 | somatic clone, with Scer\GAL4^Act5C.PI

Alk^{EC.UAS.Tag:MYC}

embryonic/larval digestive system

Alk¹

embryonic/larval gut | first instar larval stage, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

Alk^{dsRNA.WIZ.UAS}

embryonic/larval midgut

Alk¹

embryonic myoblast

Alk¹⁰

embryonic somatic muscle cell, with Scer\GAL4^twi.PG

Alk^UAS.cLa

embryonic visceral muscle cell, with Scer\GAL4^twi.PG

Alk^UAS.cLa

eye, with Scer\GAL4^sev.EP

Alk^UAS.cLa

eye, with Scer\GAL4^sev.PU

Alk^UAS.cLa

hemocyte, with Scer\GAL4^He.PZ

hemocyte, with Scer\GAL4^Hml.PG

Alk^{act.UAS.Tag:HA}

lamellocyte, with Scer\GAL4^He.PZ

Alk^UAS.cLa

lamina monopolar neuron L3 | absent | adult stage, with Scer\GAL4^GMR9B08

Alk^{dsRNA.UAS.cUa}

longitudinal visceral muscle primordium | embryonic stage 13

Alk¹

Alk^ts

muscle founder cell & circular visceral muscle primordium

Alk^mili

muscle founder cell & visceral mesoderm, with Scer\GAL4^twi.PG

Alk^UAS.cLa

muscle founder cell & visceral muscle primordium

Alk^mili

muscle founder cell & visceral muscle primordium, with Scer\GAL4^twi.2PE

Alk^UAS.DN

muscle founder cell & visceral muscle primordium | ectopic

Alk^bap3.PL

muscle founder cell | embryonic stage

Alk¹

neuromuscular junction

Alk¹

ommatidium | adult stage, with Scer\GAL4^sev.PU

Alk^UAS.cLa

optic cartridge | somatic clone

Alk¹

prothoracic gland | larval stage, with Scer\GAL4^spok.1.45

Alk^{act.UAS.Tag:HA}

visceral mesoderm

visceral mesoderm, with Scer\GAL4^twi.PG

Alk^{EC.UAS.Tag:MYC}

wing, with Scer\GAL4^Ras64B.PW

wing disc | somatic clone, with Scer\GAL4^Act5C.PI

Alk^UAS.cLa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

16

11 of 15

No

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (7)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

9 of 15

No

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

Rattus norvegicus (Norway rat) (6)

8 of 13

Yes

Yes

4 of 13

No

Yes

2 of 13

No

No

Rnor\LOC103694423

1 of 13

No

No

Rnor\LOC108351182

1 of 13

No

Yes

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (5)

3 of 12

Yes

Yes

2 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

Yes

1 of 12

No

No

Danio rerio (Zebrafish) (7)

10 of 15

Yes

Yes

3 of 15

No

Yes

2 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (5)

11 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

Arabidopsis thaliana (thale-cress) (6)

1 of 9

Yes

No

1 of 9

Yes

Yes

1 of 9

Yes

Yes

1 of 9

Yes

Yes

1 of 9

Yes

Yes

1 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

1 of 15

Yes

No

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (19)

Gene Symbol

Score

Source

Alignment

4 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 6 )

Allele

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Gouzi et al., 2011)

modeled by Nf1^E1, Nf1^E2

(Gouzi et al., 2011)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Gouzi et al., 2011)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013, Gouzi et al., 2011)

Alk^{EC.UAS.Tag:MYC}

ameliorates neurofibromatosis

modeled by Nf1^E2

(Gouzi et al., 2011)

modeled by Nf1^JF01866

(Georganta et al., 2021)

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ALK; ALK receptor tyrosine kinase

12 of 15

NEUROBLASTOMA, SUSCEPTIBILITY TO, 3; NBLST3

LTK; leukocyte receptor tyrosine kinase

11 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

(Wolfstetter et al., 2017)

anti bait coimmunoprecipitation, western blot, enzyme linked immunosorbent assay, affinity technology, fluorescence microscopy, inferred by author, tag visualisation

(Englund et al., 2003, Lee et al., 2003)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Wolfstetter et al., 2017)

suppressible

(Bai and Sehgal, 2015)

enhanceable

(Pan and O'Connor, 2021)

enhanceable

(Pan and O'Connor, 2021)

suppressible

(Pan and O'Connor, 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Pan and O'Connor, 2021)

suppressible

(Walker et al., 2013, Gouzi et al., 2011)

enhanceable

(Pan and O'Connor, 2021)

enhanceable

(Pan and O'Connor, 2021)

enhanceable

(Pan and O'Connor, 2021)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0040505

MIST (genetic) - An integrated Molecular Interaction Database

Alk

MIST (protein-protein) - An integrated Molecular Interaction Database

Alk

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-80

Cytogenetic map

Sequence location

2R:16,625,293..16,640,011 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

53C12-53C13

Limits computationally determined from genome sequence between P{PZ}Sema-2a⁰³⁰²¹&P{PZ}Cdk4⁰⁵⁴²⁸ and P{EP}BEST:SD02913^EP2148&P{lacW}Dek^k09907

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

53-53

(determined by in situ hybridisation)

(Loren et al., 2001)

53C-53D

(Palmer, 2000.2.17)

Experimentally Determined Recombination Data

Location

2-80

(FlyBase, 2016)

2-83.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

92968

P{UAS-Alk.DN}2B, w^*

25509

w¹¹¹⁸; Mi{ET1}Alk^MB06458

27518

y¹ v¹; P{TRiP.JF02668}attP2

54555

y¹ w^*; Mi{MIC}Alk^MI10448

v107083

P{KK102942}VIE-260B

v11446

w¹¹¹⁸ P{GD42}v11446

v342449

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01437}attP40/CyO-GFP

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (20)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LP03070

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040505

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP12161

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040505

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53425

Antibody Information

Laboratory Generated Antibodies

polyclonal antibody

(Stute et al., 2004)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Alk CG8250

(Palmer, 2000.5.27)

Additional comments

Other Comments

Alk is required in target neurons in the lamina for the correct formation of adult R cell projections.

(Bazigou et al., 2007)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Named 'Alk' / 'Anaplastic lymphoma kinase' because of shared homology with mammalian ALK.

(Loren et al., 2001)

Identification

External Crossreferences and Linkouts ( 51 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53425

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KJ08

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KJ08

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040505

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53425/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040505

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Alk_1
Alk_2

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040505

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040505

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8250

Flygut - An atlas of the Drosophila adult midgut

FBgn0040505

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53425

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8250

MARRVEL_MODEL - MARRVEL (model organism gene)

53425

modMine - A data warehouse for the modENCODE project

FBgn0040505

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0040505

DRSC - Results frm RNAi screens

FBgn0040505

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040505

FlyMine - An integrated database for Drosophila genomics

FBgn0040505

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

Alk

MIST (protein-protein) - An integrated Molecular Interaction Database

Alk

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

ALK

(Lanet and Maurange, 2014, Michaut et al., 2011, Schönherr et al., 2011, Wolfstetter et al., 2009, Grabbe et al., 2004)

Alk

(Hertenstein et al., 2021, Kumar et al., 2021, Mendoza-Garcia et al., 2021, Pan and O'Connor, 2021, Pang et al., 2021, Park et al., 2021, Park et al., 2021, Bakshi and Joshi, 2020, Chou et al., 2020, Lathen et al., 2020, Mariano et al., 2020, Nye et al., 2020, Orthofer et al., 2020, Pan et al., 2020, Rushton et al., 2020, Texada et al., 2020, Mele and Johnson, 2019, Pahl et al., 2019, Neuert et al., 2017, Tomita et al., 2017, Wolfstetter et al., 2017, Rohde et al., 2016, Strigini and Leulier, 2016, Yadav et al., 2016, Bai and Sehgal, 2015, Grotewiel and Bettinger, 2015, Nässel et al., 2015, Hugosson et al., 2014, Lanet and Maurange, 2014, Mulakkal et al., 2014, Pecot et al., 2014, Salazar-Jaramillo et al., 2014, Hallberg and Palmer, 2013, Jin et al., 2013, Koyama et al., 2013, Kwon et al., 2013, Muñoz-Soriano et al., 2013, Pecot et al., 2013, Sopko and Perrimon, 2013, Dani et al., 2012, Grewal, 2012, Homem and Knoblich, 2012, Reim et al., 2012, Rodriguez et al., 2012, Gouzi et al., 2011, Hadjieconomou et al., 2011, Eriksson et al., 2010, Rohrbough and Broadie, 2010, Leatherbarrow and Halfon, 2009, Bazigou et al., 2007, Bazigou et al., 2007, Popichenko et al., 2007, Shirinian et al., 2007, Varshney and Palmer, 2007, Bartolome and Charlesworth, 2006, Varshney and Palmer, 2006, Loren et al., 2003)

CG8250

(Neuert et al., 2017, Vogel et al., 2013, Keleman et al., 2009.8.5, Perkins et al., 2009, Morrison et al., 2000, Palmer, 2000.5.27)

DAlk

(Weiss et al., 2006, Loren et al., 2003, Loren et al., 2003, Weiss et al., 2003, Loren et al., 2001, Loren et al., 2001, Loren et al., 2001)

DAlk53

(Loren et al., 2001)

alk

(Lanet and Maurange, 2014, Popichenko et al., 2013, Rohrbough et al., 2013)

dALK

(Walker et al., 2013, Loren et al., 2001)

dAlk

(Yang et al., 2007)

mili

(Stute et al., 2004)

Name Synonyms

Alk

Anaplastic Lymphoma Kinase

(Mirth and Shingleton, 2012, Shirinian et al., 2007)

Anaplastic lymphoma kinase

(Mele and Johnson, 2019, Tomita et al., 2017, Lanet and Maurange, 2014, Pecot et al., 2013, Reim et al., 2012, Rohrbough and Broadie, 2010, Varshney and Palmer, 2007, Varshney and Palmer, 2006)

anaplastic lymphoma kinase

(Pecot et al., 2014, Koyama et al., 2013, Grewal, 2012, Kaun et al., 2012, Franco et al., 2011, Schönherr et al., 2011, Popichenko et al., 2007)

Secondary FlyBase IDs

FBgn0034146

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (139)