Gene Dmel\ksh

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General Information

Symbol

Dmel\ksh

Species

D. melanogaster

Name

kish

Annotation symbol

CG14199

Feature type

protein_coding_gene

FlyBase ID

FBgn0040890

Gene Model Status

Current

Stock availability

2 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

18C8-18C8

Sequence location

X:19,388,453..19,389,025 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene kish is referred to in FlyBase by the symbol Dmel\ksh (CG14199, FBgn0040890). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: intracellular transport. One allele is reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein of unknown function DUF1242. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1630246_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG14199. Gene sequence location is X:19388453..19389025.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

18C8-18C8

Limits computationally determined from genome sequence between P{EP}EP1526&P{EP}Pfrx^EP1150 and P{EP}Tao-1^EP1455

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ksh for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ksh-RB

FBtr0300214

NM_144242

435

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ksh-PB

FBpp0289451

8.2

10.15

NP_652499

AAF48961

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein of unknown function DUF1242 (IPR009653)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9VWH8

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

endoplasmic reticulum

inferred from direct assay

(Wendler et al., 2010)

Golgi apparatus

inferred from direct assay

(Wendler et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		(860.9)
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		(860.9)
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		169.35
Larval Midgut		175.4
Larval Hindgut		157.5
Larval Malpighian Tubules		133.3
Larval Fat Body		180.1
Larval Salivary Gland		860.9
Larval Trachea		200.45
Larval Carcass		146.175
Adult Head		127.3
Adult Eye		126.8
Adult Brain		132.7
Adult Thoracic-Abdominal Ganglion		125.6
Adult Crop		122.8
Adult Midgut		178.5
Adult Hindgut		152.8
Adult Malpighian Tubules		155.4
Adult Fat Body		152.6
Adult Salivary Gland		342.9
Adult Heart		127.65
Adult VirginFemale Spermatheca		113.6
Adult InseminatedFemale Spermatheca		109.3
Adult Ovary		251.3
Adult Testis		79.3
Adult Male Accessory Gland		435.1
Adult Carcass		167
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	127.3
NA	Eye	126.8
NA	Brain	132.7
169.35	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	125.6
NA	Crop	122.8
175.4	Midgut	178.5
157.5	Hindgut	152.8
133.3	Malpighian Tubules	155.4
180.1	Fat Body	152.6
860.9	Salivary Gland	342.9
NA	Heart	127.65
200.45	Trachea	NA
NA	VirginFemale Spermatheca	113.6
NA	InseminatedFemale Spermatheca	109.3
NA	Ovary	251.3
NA	Testis	79.3
NA	Male Accessory Gland	435.1
146.175	Carcass	167

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(34)
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		(28)
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		(45)
ovary, mated 4-day female		(35)
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(34)
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		(45)
ovary, mated 4-day female		(35)
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		34
central nervous system, larvae L3		17
central nervous system, pupae P8		8
head, virgin 1-day female		8
head, virgin 4-day female		10
head, virgin 20-day female		10
head, mated 1-day female		8
head, mated 4-day female		7
head, mated 20-day female		7
head, mated 1-day male		7
head, mated 4-day male		12
head, mated 20-day male		21
salivary gland, larvae L3 wandering		18
salivary gland, white prepupae		8
digestive system, larvae L3 wandering		17
digestive system, 1-day adult		13
digestive system, 4-day adult		13
digestive system, 20-day adult		14
fat body, larvae L3 wandering		9
fat body, white prepupae		16
fat body, pupae P8		28
carcass, larvae L3 wandering		24
carcass, 1-day adult		6
carcass, 4-day adult		9
carcass, 20-day adult		9
ovary, virgin 4-day female		45
ovary, mated 4-day female		35
testis, mated 4-day male		19
accessory gland, mated 4-day male		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(58)
embryo 02-04hr		13
embryo 04-06hr		(33)
embryo 06-08hr		(49)
embryo 08-10hr		(44)
embryo 10-12hr		(41)
embryo 12-14hr		(39)
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		(33)
larva L2		(48)
larva L3 12hr old		(33)
larva L3 puffstage 1-2		(39)
larva L3 puffstage 3-6		(42)
larva L3 puffstage 7-9		(39)
white prepupae new		(44)
white prepupae 12hr		(35)
white prepupae 24hr		(46)
pupae 2d postWPP		(40)
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		(27)
adult male 05day		26
adult male 30day		(27)
adult female 01day		(38)
adult female 05day		(67)
adult female 30day		(60)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(58)
embryo 02-04hr		13
embryo 04-06hr		(33)
embryo 06-08hr		(49)
embryo 08-10hr		(44)
embryo 10-12hr		(41)
embryo 12-14hr		(39)
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		(33)
larva L2		(48)
larva L3 12hr old		(33)
larva L3 puffstage 1-2		(39)
larva L3 puffstage 3-6		(42)
larva L3 puffstage 7-9		(39)
white prepupae new		(44)
white prepupae 12hr		(35)
white prepupae 24hr		(46)
pupae 2d postWPP		(40)
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		(38)
adult female 05day		(67)
adult female 30day		(60)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		58
embryo 02-04hr		13
embryo 04-06hr		33
embryo 06-08hr		49
embryo 08-10hr		44
embryo 10-12hr		41
embryo 12-14hr		39
embryo 14-16hr		24
embryo 16-18hr		20
embryo 18-20hr		22
embryo 20-22hr		21
embryo 22-24hr		17
larva L1		33
larva L2		48
larva L3 12hr old		33
larva L3 puffstage 1-2		39
larva L3 puffstage 3-6		42
larva L3 puffstage 7-9		39
white prepupae new		44
white prepupae 12hr		35
white prepupae 24hr		46
pupae 2d postWPP		40
pupae 3d postWPP		25
pupae 4d postWPP		20
adult male 01day		27
adult male 05day		26
adult male 30day		27
adult female 01day		38
adult female 05day		67
adult female 30day		60
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(44)
Schneider line 2 Sg4		(47)
embryonic 1182-4H		(35)
embryonic GM2		(43)
embryonic Kc167		18
embryonic S1		(53)
embryonic S3		(44)
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		(33)
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		(39)
wing disc ML-DmD32		(27)
haltere disc ML-DmD17-c3		(53)
eye-antennal disc ML-DmD11		(39)
antennal disc ML-DmD20-c5		(57)
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		(32)
CNS ML-DmBG2-c2		(56)
tumorous blood cells mbn2		(30)
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(44)
Schneider line 2 Sg4		(47)
embryonic 1182-4H		(35)
embryonic GM2		(43)
embryonic Kc167		18
embryonic S1		(53)
embryonic S3		(44)
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		(33)
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		(39)
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		(53)
eye-antennal disc ML-DmD11		(39)
antennal disc ML-DmD20-c5		(57)
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		(56)
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		44
Schneider line 2 Sg4		47
embryonic 1182-4H		35
embryonic GM2		43
embryonic Kc167		18
embryonic S1		53
embryonic S3		44
leg disc CME L1		24
wing disc CME-W2		26
wing disc ML-DmD8		33
wing disc ML-DmD9		20
wing disc ML-DmD16-c3		21
wing disc ML-DmD21		39
wing disc ML-DmD32		27
haltere disc ML-DmD17-c3		53
eye-antennal disc ML-DmD11		39
antennal disc ML-DmD20-c5		57
mixed discs ML-DmD4-c1		23
CNS ML-DmBG1-c1		32
CNS ML-DmBG2-c2		56
tumorous blood cells mbn2		30
ovary fGS/OSS		12
ovary OSC		13
ovary OSS		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		(27)
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		(36)
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		(29)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		(27)
Paraquat 10 mM 48 hrs, 4-day adult		(32)
Rotenone 2 μg 12 hrs, larvae L3		(28)
Rotenone 8 μg 12 hrs, larvae L3		(40)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		(36)
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		(34)
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		(40)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		6
cold shock, 4-day adult		5
heat shock, 4-day adult		25
Cadmium 50 mM 6 hrs, larvae L3		23
Cadmium 50 mM 12 hrs, larvae L3		27
Cadmium 50 mM 48 hrs, 4-day adult		21
Cadmium 100 mM 48 hrs, 4-day adult		36
Copper 0.5 mM 12 hrs, larvae L3		12
Copper 15 mM 48 hrs, 4-day adult		7
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		8
Ethanol 2.5% 3 hrs, larvae L3		34
Ethanol 5% 3 hrs, larvae L3		20
Ethanol 10% 3 hrs, larvae L3		29
Caffeine 1.5 mg/ml 4 hrs, larvae L3		21
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		22
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		27
Paraquat 10 mM 48 hrs, 4-day adult		32
Rotenone 2 μg 12 hrs, larvae L3		28
Rotenone 8 μg 12 hrs, larvae L3		40
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0040890

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG14199-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

ksh^GD4659

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of ksh	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ksh	Class	Mutagen	Stocks	Known lesion
ksh^GD4659		in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes

Aneuploid Aberrations

Disrupted in

Df(1)Exel7468

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{GT1}Pfrx^BG02836

insertion of mobile activating element

P{EPgy2}Pfrx^EY05435

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

There is no information supporting a molecular function term for this gene.

(Based on a review of available data before: 2006)

Terms Based on Experimental Evidence ( 3 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

intracellular transport

inferred from mutant phenotype

(Wendler et al., 2010)

Cellular Component

CV term

Evidence

References

endoplasmic reticulum

inferred from direct assay

(Wendler et al., 2010)

Golgi apparatus

inferred from direct assay

(Wendler et al., 2010)

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ksh allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

72652

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0040890

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

50363

Orthologs

OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG68WCNW

Dipteran inclusive ortholog search

EOG6DZ250

Insect inclusive ortholog search

EOG679D57

Arthropod inclusive ortholog search

EOG6C5B2K

Metazoa inclusive ortholog search

EOG6ZPSRN

Orthologs in Drosophila Species (EOG68WCNW)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12819

Drosophila persimilis

Dper\GL14781

Drosophila willistoni

Dwil\GK25511

Drosophila virilis

Dvir\GJ18294

Drosophila mojavensis

Dmoj\GI16437

Drosophila grimshawi

Dgri\GH17869

Orthologs in non-Drosophila Dipterans (EOG6DZ250)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000407

Anopheles gambiae

Malaria mosquito

Agam\AGAP002087

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002853

Orthologs in non-Dipteran Insects (EOG679D57)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55473

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG033366

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH16384

Atta cephalotes

Leafcutter ant

Acep\ACEP20167

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL16873

Linepithema humile

Argentine ant

Lhum\LH18566

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB14853

Solenopsis invicta

Red fire ant

Sinv\SINV20239

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI50889

Pediculus humanus

Human body louse

Phum\PHUM399380

Orthologs in non-Insect Arthropods (EOG6C5B2K)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\COI

Ixodes scapularis

Deer tick

Isca\ISCW010144

Orthologs in non-Arthropod Metazoa (EOG6ZPSRN)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Xenopus tropicalis

Western clawed frog

Xtro\tmem167a

Gallus gallus

Domestic chicken

Ggal\TMEM167A

Mus musculus

House mouse

Mmus\Tmem167a

Mus musculus

House mouse

Mmus\Gm10495

Rattus norvegicus

Norway rat

Rnor\LOC100359823

Homo sapiens

Human

Hsap\TMEM167A

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\TMEM167A

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12819

Drosophila persimilis

Dper\GL14781

Drosophila willistoni

Dwil\GK25511

Drosophila virilis

Dvir\GJ18294

Drosophila mojavensis

Dmoj\GI16437

Drosophila grimshawi

Dgri\GH17869

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v40884

w¹¹¹⁸; P{GD4659}v40884

v32051

w¹¹¹⁸; P{GD4659}v32051/CyO

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 3 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

IP03267

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0040890

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

50363

Antibody Information

Other Information

Discoverer

Etymology

The gene is named "kish", transliterated from the Hungarian for small, as the encoded protein is only 72 residues.

(Wendler et al., 2010)

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: ksh CG14199

(Wendler et al., 2010)

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_144242

RefSeq (Proteins)

•

NP_652499

DDBJ / EMBL / GenBank

•

AAF48961

Entrez Gene - A searchable database of RefSeq genes.

•

50363

UniProtKB/Swiss-Prot

•

Q9VWH8

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein of unknown function DUF1242 (IPR009653)

Linkouts

BioGRID - A database of protein and genetic interactions

•

72652

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0040890

DRSC - Results from RNAi screens.

•

FBgn0040890

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0040890

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG14199-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0040890

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

50363

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

50363

modMine - Data generated by the modENCODE project.

•

FBgn0040890

Synonyms & Secondary IDs ( 3 )

Reported As

Symbol Synonym

CG14199

(Wendler et al., 2010, Ayroles et al., 2009)

ksh

(Wendler et al., 2010, )

Name Synonym

kish

(Wendler et al., 2010, )

Secondary FlyBase IDs

References ( 10 )

Research paper

Wendler et al., 2010, EMBO J. 29(2): 304--314: A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway. [FBrf0209739]
Ayroles et al., 2009, Nat. Genet. 41(3): 299--307: Systems genetics of complex traits in Drosophila melanogaster. [FBrf0206705]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Gene Disruption Project members, 2001-, (Computer file): (Computer file) [FBrf0132177]

FlyBase analysis

FlyBase Curators, 2002-, Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase.: Use of the ND evidence code for Gene Ontology (GO) terms in FlyBase. [FBrf0159398]