FlyBase Gene Report: Dmel\wun2

General Information

Symbol

Dmel\wun2

Species

D. melanogaster

Name

wunen-2

Annotation Symbol

CG8805

Feature Type

protein_coding_gene

FlyBase ID

FBgn0041087

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

Phosphatidate phosphatase (3.1.3.4)

Gene Summary

wunen-2 (wun2) encodes a lipid phosphate phosphatase, an enzyme with an active site predicted to face extracellularly. It is required for germ cell migration and survival, acting partially redundantly with its paralog wun. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

wun-2, N14, Tunen, Pap2G

Key Links

Genomic Location

Cytogenetic map

45D4-45D5

Sequence location

2R:9,414,342..9,417,846 [+]

Recombination map

2-60

RefSeq locus

NT_033778 REGION:9414342..9417846

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables lipid phosphatase activity

inferred from direct assay

(Renault et al., 2004)

enables lipid transporter activity

inferred from direct assay

(Renault et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables lipid phosphatase activity

inferred from biological aspect of ancestor with PANTHER:PTN000807400

(assigned by GO_Central )

(Gaudet et al., 2011)

enables phosphatidate phosphatase activity

inferred from sequence or structural similarity with FLYBASE:wun; FB:FBgn0016078

(Starz-Gaiano et al., 2001)

inferred from biological aspect of ancestor with PANTHER:PTN000017950

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (9 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in germ cell development

inferred from mutant phenotype

(Renault et al., 2004)

involved_in germ cell migration

inferred from mutant phenotype

(Starz-Gaiano et al., 2001)

inferred from genetic interaction with FLYBASE:wun; FB:FBgn0016078

(Starz-Gaiano et al., 2001)

involved_in germ cell repulsion

inferred from genetic interaction with FLYBASE:wun; FB:FBgn0016078

(Renault et al., 2010)

involved_in pole cell development

inferred from mutant phenotype

(Hanyu-Nakamura et al., 2004)

involved_in pole cell migration

inferred from mutant phenotype

(Hanyu-Nakamura et al., 2004)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in ectopic germ cell programmed cell death

traceable author statement

(Coffman, 2003)

involved_in germ cell migration

traceable author statement

(Müller, 2002)

involved_in germ cell repulsion

traceable author statement

(Raz, 2004, Coffman, 2003, Luquain et al., 2003)

involved_in phospholipid dephosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000807400

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in phospholipid metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000807400

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000807400

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in apical part of cell

inferred from direct assay

(Ile et al., 2012)

located_in plasma membrane

inferred from direct assay

(Starz-Gaiano et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000807400

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

LIPID PHOSPHATE PHOSPHATASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

A 1,2-diacylglycerol 3-phosphate + H(2)O = a 1,2-diacyl-sn-glycerol + phosphate (3.1.3.4)

Summaries

Gene Snapshot

Gene Group (FlyBase)

LIPID PHOSPHATE PHOSPHATASES -: Lipid phosphate phosphatases (LPP) are integral membrane proteins, with six putative transmembrane domains, that catalyze the dephosphorylation of a variety of lipid phosphates. (Adapted from FBrf0191316 and PMID:25814022).

Summary (Interactive Fly)

lipid phosphate phosphohydrolases - somatic Wun and Wun2 provide a repulsive environment for germ cell migration by depleting an extracellular, attractive substrate - in germ cells Wun2 competes with somatic Wun and Wun2 for a common lipid phosphate substrate, which is required by germ cells to produce a survival signal

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\wun2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KGH1)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

wun2-RA

FBtr0088506

NM_080252

1968

350

wun2-RB

FBtr0339380

NM_001299319

2211

246

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

wun2-PA

FBpp0087590

39.5

350

8.58

FBpp0308471

28.4

246

8.54

A0A0B4LEJ6

NP_001286248

AHN56046

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wun2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wun2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

posterior midgut primordium | restricted

Comment: excludes part where germ cells exit the gut

(Starz-Gaiano et al., 2001)

hindgut proper primordium

(Starz-Gaiano et al., 2001)

anterior midgut primordium

(Starz-Gaiano et al., 2001)

embryonic stage 10 -- 12

neuroblast | lateral | subset

(Starz-Gaiano et al., 2001)

embryonic stage 10 -- 13

epidermis | segmentally repeated

(Starz-Gaiano et al., 2001)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical part of cell

inferred from direct assay

(Ile et al., 2012)

located_in plasma membrane

inferred from direct assay

(Starz-Gaiano et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\wun2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\wun2

View exonic expression by tissue for Dmel\wun2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53558/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041087

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041087

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041087

Flygut - An atlas of the Drosophila adult midgut

FBgn0041087

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 15 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of wun2

Transgenic constructs containing regulatory region of wun2

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(2R)11

(Georgiev et al., 2012)

Df(2R)BSC29

(Georgiev et al., 2012)

Df(2R)Np5

(Georgiev et al., 2012, Hanyu-Nakamura et al., 2004)

Df(2R)NYX-D15

(Sano et al., 2005)

Df(2R)w45-19g

(Georgiev et al., 2012, Hanyu-Nakamura et al., 2004)

Df(2R)w45-30n

(Hanyu-Nakamura et al., 2004)

Df(2R)w73-1

(Hanyu-Nakamura et al., 2004)

Df(2R)wun-GL

(Sano et al., 2005, Hanyu-Nakamura et al., 2004)

Gene not disrupted in

Df(2R)Np1

(Hanyu-Nakamura et al., 2004)

Df(2R)w73-1

(Georgiev et al., 2012)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

wun2^GD1900

viable, with Scer\GAL4^- :

wun2^EP2527

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

wun2^GD1900

viable, with Scer\GAL4^tin.CΔ4

wun2^GD1900

viable (with Df(2R)w45-19g)

wun2^N14

Sterility

Allele

sterile, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

wun2^EP2217

Other Phenotypes

Allele

abnormal cell number | embryonic stage | maternal effect

wun2^N14

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

wun2^EP2607

decreased cell number | embryonic stage 15, with Scer\GAL4^twi.PG

wun2^UAS.Tag:MYC

decreased cell number | embryonic stage 15 | germline clone | maternal effect

decreased cell number | embryonic stage 15 | maternal effect (with Df(2R)w45-19g)

decreased cell number | embryonic stage 16, with Scer\GAL4^twi.PG

wun2^UAS.Tag:MYC

decreased cell number | embryonic stage 16 | germline clone | maternal effect

decreased cell number | embryonic stage 16 | maternal effect (with Df(2R)w45-19g)

increased cell death | embryonic stage, with Scer\GAL4^twi.PG

wun2^UAS.Tag:MYC

increased cell death | embryonic stage | germline clone | maternal effect

increased cell death | embryonic stage | maternal effect (with Df(2R)w45-19g)

short lived, with Scer\GAL4^Mef2.PR

wun2^GD1900

some die during pupal stage, with Scer\GAL4^Mef2.PR

wun2^GD1900

some die during pupal stage, with Scer\GAL4^pnr-MD237

wun2^GD1900

visible, with Scer\GAL4^pnr-MD237

wun2^GD1900

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

wun2^EP2607

germline cell

wun2^{αTub84B.Tag:MYC}

germline cell, with Scer\GAL4^48Y

wun2^EP2217

germline cell, with Scer\GAL4^btl.PS

wun2^EP2217

germline cell, with Scer\GAL4^da.G32

wun2^UAS.Tag:MYC

germline cell, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

germline cell, with Scer\GAL4^twi.PG

germline cell, with Scer\GAL4^VP16.nos.UTR

germline cell | embryonic stage 15, with Scer\GAL4^twi.PG

wun2^UAS.Tag:MYC

germline cell | embryonic stage 15 | germline clone | maternal effect

germline cell | embryonic stage 15 | maternal effect (with Df(2R)w45-19g)

germline cell | embryonic stage 16, with Scer\GAL4^twi.PG

wun2^UAS.Tag:MYC

germline cell | embryonic stage 16 | germline clone | maternal effect

germline cell | embryonic stage 16 | maternal effect (with Df(2R)w45-19g)

germline cell | maternal effect (with Df(2R)wun-GL)

wun2^EP2650ex34

mesothoracic tergum, with Scer\GAL4^pnr-MD237

wun2^GD1900

ovary & germ cell & adult | non-rescuable maternal effect (with Df(2R)w45-19g)

wun2^N14

pole cell & gonad & embryo | non-rescuable maternal effect (with Df(2R)w45-19g)

wun2^N14

primordial germ cell | embryonic stage, with Scer\GAL4^twi.PG

wun2^EP2650

primordial germ cell | embryonic stage, with Scer\GAL4^VP16.nos.UTR

wun2^EP2650

primordial germ cell | embryonic stage | maternal effect

wun2^N14

primordial germ cell | embryonic stage | non-rescuable maternal effect (with Df(2R)w45-19g)

wun2^N14

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PLPP1

12 of 15

Yes

Hsap\PLPP3

12 of 15

Yes

Hsap\PLPP2

11 of 15

Yes

4 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

1 of 15

Yes

Hsap\SGPP1

1 of 15

Yes

Hsap\SGPP2

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (13)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Plpp1

13 of 15

Yes

Mmus\Plpp2

11 of 15

Yes

Mmus\Plpp3

11 of 15

Yes

4 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

1 of 15

Yes

Mmus\Sgpp1

1 of 15

Yes

Mmus\Sgpp2

1 of 15

Yes

Rattus norvegicus (Norway rat) (13)

Rnor\Plpp3

12 of 13

Yes

Rnor\Plpp1

10 of 13

Yes

Rnor\Plpp2

10 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Rnor\Sgpp2

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (12)

Xtro\plpp2

11 of 12

Yes

Xtro\plpp3

11 of 12

Yes

Xtro\LOC100135382

7 of 12

Yes

Xtro\plpp1

7 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

1 of 12

Yes

Xtro\plppr1

1 of 12

Danio rerio (Zebrafish) (21)

Drer\plpp1a

11 of 15

Yes

Drer\plpp3

11 of 15

Yes

Drer\plpp2a

10 of 15

Yes

Drer\plpp2b

10 of 15

Drer\ppap2d

10 of 15

Yes

Drer\LOC794598

9 of 15

Yes

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Drer\sgpp1

1 of 15

Yes

Drer\sgpp2

1 of 15

Yes

Drer\si:ch211-212g7.6

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\plpp-1.1

10 of 15

Yes

Cele\plpp-1.2

6 of 15

Cele\plpp-1.3

5 of 15

Yes

Cele\plpr-1

2 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\LPP2

1 of 9

Yes

Atha\LPP3

1 of 9

Yes

Atha\LPP4

1 of 9

Yes

Atha\PAP1

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\DPP1

5 of 15

Yes

Scer\LPP1

4 of 15

Scer\CAX4

1 of 15

Yes

Scer\LCB3

1 of 15

Yes

Scer\YSR3

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC409.18

3 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

8 of 10

wun

7 of 10

6 of 10

6 of 10

6 of 10

6 of 10

5 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PLPP1; phospholipid phosphatase 1

12 of 15

607124

PLPP3; phospholipid phosphatase 3

12 of 15

607125

PLPP2; phospholipid phosphatase 2

11 of 15

607126

PLPP5; phospholipid phosphatase 5

4 of 15

610626

PLPP4; phospholipid phosphatase 4

3 of 15

PLPPR1; phospholipid phosphatase related 1

3 of 15

619590

PLPPR2; phospholipid phosphatase related 2

3 of 15

619591

PLPPR3; phospholipid phosphatase related 3

3 of 15

610391

PLPPR4; phospholipid phosphatase related 4

3 of 15

607813

PLPPR5; phospholipid phosphatase related 5

3 of 15

617287

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

wun2

Hsap\PLPP3

(Renault et al., 2004)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

wun2 - Mob4

two hybrid

(Hu et al., 2017.6.13)

wun2 - Vta1

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

wun2|wun

suppressible

Rac1

(Renault et al., 2010)

wun2|wun

suppressible

Rho1

(Renault et al., 2010)

wun2

suppressible

srp

(Renault et al., 2010)

wun2|wun

suppressible

srp

(Renault et al., 2010)

wun2

suppressible

wun

(Hanyu-Nakamura et al., 2004, Renault et al., 2004)

wun2

enhanceable

wun

(Renault et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

nes

enhanceable

wun2

(Steinhauer et al., 2009)

rdgA

enhanceable

wun2

(Garcia-Murillas et al., 2006)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72790

DroID - A comprehensive database of gene and protein interactions.

FBgn0041087

MIST (genetic) - An integrated Molecular Interaction Database

wun2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

diacylglycerol and triacylglycerol biosynthesis

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0041087

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sphingolipid de novo biosynthesis

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-60

Cytogenetic map

45D4-45D5

Sequence location

2R:9,414,342..9,417,846 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

45D4-45D5

Limits computationally determined from genome sequence between P{lacW}wun^k09507&P{lacW}l(2)k12402^k12402 and P{EP}EP2165&P{lacW}l(2)k01301^k01301

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

45D-45D

(Hanyu, 2002.3.27, Hayden, 2000.2.17)

45C-45D

(Hanyu et al., 2002)

Experimentally Determined Recombination Data

Location

2-60

(FlyBase, 2016)

2-59.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

59592

y¹ w^*; Mi{MIC}wun2^MI15037

14689

y¹ w^67c23; P{SUPor-P}wun2^KG08150

77295

y¹ sc^* v¹ sev²¹; P{TKO.GS01135}attP40

85763

y¹ sc^* v¹ sev²¹; P{TOE.GS01591}attP40

32423

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00418}attP2

32381

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00372}attP2/TM3, Sb¹

Kyoto

113067

w^*; P{GawB}wun2^NP3026 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v103452

P{KK113349}VIE-260B

v4176

w¹¹¹⁸; P{GD1900}v4176

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (95)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE26896

Other clones

FI04477

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0041087

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0041087

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53558

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mukherjee et al., 2013)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Tunen CG8805

(Bayraktaroglu, 2000.8.7)

Source for merge of: wun2 Tunen

(Lehmann and Starz-Gaiano, 2000.11.28)

Source for merge of: Tunen BEST:CK02248

(Bayraktaroglu, 2000.11.2)

Source for merge of: wun2 Pap2G

(Howard, 2002.5.8)

Source for merge of: wun2 N14

(Hanyu-Nakamura et al., 2004)

Additional comments

Other Comments

The Hrp59 protein binds preferentially to a subset of mRNAs, including wun2 mRNA.

(Kiesler et al., 2005)

wun2 may provide a repulsive environment for pole cell migration.

(Hanyu-Nakamura et al., 2004)

wun2 functions in germ cells in the embryo, enabling them to perceive the wun/wun2-related signal from the soma.

(Renault et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: genetic screen for mutants affecting pole cell development.

(Hanyu et al., 2002)

Embryos lacking maternal wun2 function form normal number of pole cells. However, most of these pole cells are lost during their migration to the gonads, and no or few pole cells are incorporated into the gonads. No defect is observed in somatic cell development, and the mutant embryos develop into sterile adults.

(Hanyu et al., 2002)

wun2 acts as a repellent for migrating primordial germ cells. wun and wun2 appear to act redundantly.

(Starz-Gaiano et al., 2001)

Identification: in an over-expression screen for genes which effect germ cell migration.

(Starz-Gaiano et al., 2000)

wun and wun2 cooperate to guide the germ cells by acting as repellants.

(Starz-Gaiano et al., 2000)

Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

(Kopczynski et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53558

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KGH1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KGH1

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0041087

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53558/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041087

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wun2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041087

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041087

Flygut - An atlas of the Drosophila adult midgut

FBgn0041087

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0041087

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

53558

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8805

MARRVEL_MODEL - MARRVEL (model organism gene)

53558

modMine - A data warehouse for the modENCODE project

FBgn0041087

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

11015-wunen-2[k11120a]

BioGRID - A database of protein and genetic interactions.

72790

DroID - A comprehensive database of gene and protein interactions.

FBgn0041087

DRSC - Results frm RNAi screens

FBgn0041087

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0041087

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

diacylglycerol and triacylglycerol biosynthesis

FlyMine - An integrated database for Drosophila genomics

FBgn0041087

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

wun2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Sphingolipid de novo biosynthesis

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

BEST:CK02248

CG8805

(Wang et al., 2019, Hosono et al., 2015, Ibrahim et al., 2013, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Muse et al., 2007, Valanne et al., 2007, Brody et al., 2002, Morrison et al., 2000)

CK02248

(Kopczynski et al., 1998)

DrPAP2^G

(Hayden and Howard, 1999)

LPP-like

(Garcia-Murillas et al., 2006)

N14

(Hanyu-Nakamura et al., 2004, Hanyu, 2002.3.27, Hanyu et al., 2002)

Pap2G

(Hayden, 1999.3.3)

Tunen

(Kraut et al., 2001, Kraut et al., 2001, Hayden, 2000.2.17)

WUN-like

(Morrison et al., 2000)

Wun2

(Steinhauer et al., 2009)

Wunen-2

(Zhang et al., 2021)

wun-2

(Thanh et al., 2020, Georgiev et al., 2012, Sano et al., 2005, Santos and Lehmann, 2004, Starz-Gaiano and Lehmann, 2001)

wun2

(Lehmann, 2021, Slaidina et al., 2021, Tu et al., 2021, Trcek et al., 2020, Wat et al., 2020, Brunet Avalos et al., 2019, Hanyu-Nakamura et al., 2019, Shah et al., 2019, Trcek and Lehmann, 2019, Croset et al., 2018, Hu et al., 2017.6.13, LeBlanc and Lehmann, 2017, Transgenic RNAi Project members, 2017-, Huang et al., 2015, Katsuyama et al., 2015, Kwon et al., 2013, Zhu and Zhang, 2013, Ile et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Smith et al., 2011, Renault et al., 2010, Bao and Friedrich, 2009, Gilchrist et al., 2008, Kwon et al., 2008, Adryan et al., 2007, Buszczak et al., 2007, Muse et al., 2007, Tao et al., 2007, Choksi et al., 2006, Garcia-Murillas et al., 2006, Garcia-Murillas et al., 2006, Montell, 2006, Renault and Lehmann, 2006, Kiesler et al., 2005, Xavier-Neto, 2005, Brodsky et al., 2004, Renault et al., 2004, Renault et al., 2004, Burnett and Howard, 2003)

Name Synonyms

Wunen 2

(Rice et al., 2021, Sano et al., 2005)

Wunen2

(Jacquemyn et al., 2021, Burnett and Howard, 2003, Kraut et al., 2001, Kraut et al., 2001)

wunen 2

(Renault et al., 2004)

wunen-2

(Ibrahim et al., 2013, Renault et al., 2010, Bao and Friedrich, 2009, Garcia-Murillas et al., 2006, Garcia-Murillas et al., 2006, Sano et al., 2005, Starz-Gaiano et al., 2000)

wunen2

(Osman and Pek, 2021, Steinhauer et al., 2009, Bakal et al., 2008, Tao et al., 2007, Montell, 2006, Renault and Lehmann, 2006, Raymond et al., 2004, Renault et al., 2002)

Secondary FlyBase IDs

FBgn0025690
FBgn0026382
FBgn0033411
FBgn0040264
FBgn0062263

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (135)

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