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Gene Dmel\rols

General Information
Symbol	Dmel\rols	Species	D. melanogaster
Name	rolling pebbles	Annotation symbol	CG32096
Feature type	protein_coding_gene	FlyBase ID	FBgn0041096
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	Ants, Rols7, l(3)08232
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	68F1-68F1	Sequence location	3L:12,001,325..12,057,959 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene rolling pebbles is referred to in FlyBase by the symbol Dmel\rols (CG32096, FBgn0041096). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: myoblast fusion; anterior Malpighian tubule development; border follicle cell migration. 47 alleles are reported. The phenotypes of these alleles are annotated with: embryonic anterior Malpighian tubule; embryonic/larval pharynx; embryonic Malpighian tubule; Malpighian tubule main body primordium; presumptive embryonic/larval muscle system; embryonic somatic muscle; embryonic myoblast; midgut constriction 1; presumptive embryonic/larval digestive system. It has 9 annotated transcripts and 9 annotated polypeptides. Protein features are: Ankyrin repeat; Ankyrin repeat-containing domain; Tetratricopeptide repeat-containing domain; Tetratricopeptide-like helical; Zinc finger, RING-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult Malpighian tubules, adult spermathecae. Comments on Affy2 ProbeSet: ProbeSet 1624004_s_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of rols. Gene sequence location is 3L:12001325..12057959.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms border follicle cell migration
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 68F1-68F1   Limits computationally determined from genome sequence between P{PZ}CycA02461&P{PZ}CycA03946 and P{PZ}rols08232&P{lacW}l(3)j2D3j2D3
Experimentally Determined Cytological Location
Cytogenetic map Notes References 68E-68F     (Chen, 2001.10.29) 68F1-68F2     (Menon, 2001.8.18) 68F1-68F3   (determined by in situ hybridisation)   (Spradling et al., 1999) 68F1-68F3     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\rols for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.46 Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) rols-RA FBtr0076056  NM_168488   5505   1569 rols-RB FBtr0076053  NM_168487   6275   1900 rols-RC FBtr0076057  NM_168489   5832   1670 rols-RD FBtr0076054  NM_140285   6026   1569 rols-RE FBtr0076055  NM_168490   5849   1670 rols-RF FBtr0310009  NM_001274807   5846   1669 rols-RG FBtr0310010  NM_001274808   5972   1799 rols-RH FBtr0334109  NM_001274809   5502   1568 rols-RI FBtr0334110  NM_001274810   5969   1798
Additional Transcript Data & Comments
Reported size (kB)	7, 6 (northern blot) (Menon and Chia, 2001)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein rols-PA   FBpp0075788   169.1   1569   8.23     NP_729779   AAF49968 rols-PB   FBpp0075785   204.6   1900   6.64     NP_729778   AAF49967 rols-PC   FBpp0075789   180.1   1670   8.46     NP_729780   AAF49970 rols-PD   FBpp0075786   169.1   1569   8.23     NP_648542   AAN12250 rols-PE   FBpp0075787   180.1   1670   8.46     NP_729781   AAN12251 rols-PF   FBpp0301715   179.9   1669   8.46     NP_001261736   AGB94429 rols-PG   FBpp0301716   193.6   1799   6.40     NP_001261737   AGB94430 rols-PH   FBpp0306229   168.9   1568   8.23     NP_001261738   AGB94431 rols-PI   FBpp0306230   193.5   1798   6.40     NP_001261739   AGB94432
Additional Polypeptide Data & Comments
Reported size (kDa)	1900 (aa); 205 (kD) (Menon and Chia, 2001)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, RING-type (IPR001841) Ankyrin repeat (IPR002110) Tetratricopeptide-like helical (IPR011990) Tetratricopeptide repeat-containing domain (IPR013026) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Ankyrin repeat-containing domain (IPR020683)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA803723   AE014296 AAF49967   AE014296 AAF49968   AE014296 AAF49970   AE014296 AAN12250   AE014296 AAN12251   AF386647 AAL50557   AF386648 AAL50558   AF411457 AAL32442   AF411458 AAL32443   AF440568 AAL30170   AF440569 AAL30171   AE014296 AGB94429   AE014296 AGB94430   AE014296 AGB94431   AE014296 AGB94432   AI259518   AI292638   AI532396   AW940595   AX482651   AY058388 AAL13617   BG637610   BG639648   BI569494   BI570179   BI574084   BI574085   BI590364   CO264722   CZ470166   CZ471295   CZ473824   CZ474147   CZ485569   DU708667   EC051461   EC051854   EC051897   EC052461   EC052902   EC053001   EC053009   EC053888   EC054397   EC054404   EC056264   EC072444   G01171   GH944100   GH944180   GH953355   GH953913   GH954817   GH957971   GH962648   GH963450   GH964563   GH965465   GH966250   GH966933   GH970057   GH975876   GH976993   GH981380   GH983815
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VTS6 Q9VTS8 Q9VTS9 Q8WS23 Q8WS24 Q8WTD2 Q8WTD1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 9 -- 10   dorsal ectoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 11   presumptive embryonic/larval muscle system & myoblast | subset   (Menon and Chia, 2001) embryonic stage 11 -- 12   amnioserosa   (Fisher et al., 2012) anterior midgut primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium embryonic stage 11 -- 14   presumptive embryonic/larval muscle system | restricted   (Chen and Olson, 2001) embryonic stage 12 -- 15   myotube   (Menon and Chia, 2001) embryonic stage 13 -- 16   embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic head epidermis   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval posterior spiracle   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) embryonic stage 13 -- 17   endoderm   (Menon and Chia, 2001) embryonic stage 15   muscle attachment site   (Chen and Olson, 2001)
Additional Descriptive Data
	Transcript is first detected in the progenitors of visceral, somatic and pharyngeal muscles. Visceral mesoderm expression decreases during germband shortening while somatic mesoderm expression persists. At stage 15 rols transcript is no longer detected in the mesoderm but rather in muscle attachment sites along the segmental border. (Chen and Olson, 2001)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   presumptive embryonic/larval muscle system & myoblast | subset   • cytoplasm (Menon and Chia, 2001) embryonic stage 11 -- 14   presumptive embryonic/larval muscle system | restricted   • cytoplasm | restricted (Chen and Olson, 2001) embryonic stage 12 -- 15   myotube   • cytoplasm & plasma membrane (Menon and Chia, 2001) embryonic stage 15   muscle attachment site   • cytoplasm | restricted (Chen and Olson, 2001)
Additional Descriptive Data
	The protein is detected in discrete aggregates in the cytoplasm of founder muscle progenitor cells. (Menon and Chia, 2001, Chen and Olson, 2001)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
adherens junction inferred from direct assay (Menon et al., 2005) cell surface inferred from direct assay (Menon et al., 2005) cytoplasm inferred from direct assay (Chen and Olson, 2002, Chen and Olson, 2001, Menon et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult Malpighian tubules, adult spermathecae. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    51.775 Larval Midgut    42.3 Larval Hindgut    92.2 Larval Malpighian Tubules    87.6 Larval Fat Body    10.9 Larval Salivary Gland    32.5 Larval Trachea    32.05 Larval Carcass    33.625 Adult Head    58.1 Adult Eye    36.75 Adult Brain    54 Adult Thoracic-Abdominal Ganglion    45 Adult Crop    21.5 Adult Midgut    61.1 Adult Hindgut    77.4 Adult Malpighian Tubules    141.4 Adult Fat Body    32.6 Adult Salivary Gland    20.2 Adult Heart    19 Adult VirginFemale Spermatheca    123.4 Adult InseminatedFemale Spermatheca    144.1 Adult Ovary    17.7 Adult Testis    10.3 Adult Male Accessory Gland    82 Adult Carcass    47.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    58.1   NA  Eye    36.75   NA  Brain    54   51.775  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    45   NA  Crop    no informative data   42.3  Midgut    61.1   92.2  Hindgut    77.4   87.6  Malpighian Tubules    141.4   no informative data  Fat Body    32.6   32.5  Salivary Gland    no informative data   NA  Heart    19   32.05  Trachea    NA   NA  VirginFemale Spermatheca    123.4   NA  InseminatedFemale Spermatheca    144.1   NA  Ovary    no informative data   NA  Testis    no informative data   NA  Male Accessory Gland    82   33.625  Carcass    47.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    2 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    1 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    5 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 06-18 hour embryonic stages, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    11 embryo 08-10hr    16 embryo 10-12hr    22 embryo 12-14hr    13 embryo 14-16hr    18 embryo 16-18hr    17 embryo 18-20hr    17 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    5 larva L2    4 larva L3 12hr old    3 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    5 larva L3 puffstage 7-9    8 white prepupae new    12 white prepupae 12hr    14 white prepupae 24hr    20 pupae 2d postWPP    19 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    5 adult male 05day    5 adult male 30day    5 adult female 01day    5 adult female 05day    2 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    5 embryonic 1182-4H    0 embryonic GM2    2 embryonic Kc167    2 embryonic S1    0 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    2 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    1 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    4 ovary OSC    1 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    2 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    1 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    1 Paraquat 10 mM 48 hrs, 4-day adult    2 Rotenone 2 μg 12 hrs, larvae L3    0 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_11
External Data & Images
Linkouts	BDGP expression data • GH15583 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041096 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32096-RD FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for rols Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(3L)BK9) rols08232ex18 lethal | embryonic stage | recessive rols08232 rolsA490.2M3 lethal | recessive rols08232 rolsj2A6 partially lethal - majority live rols5a viable rolsLA00796 Sterility Allele fertile rols5a Phenotype manifest in Allele A1-7 dorsal acute muscle 1 | embryonic stage 15 (with Df(3L)BK9) rols08232ex18 border follicle cell | somatic clone rolsT627 dorsal acute muscle & myotube rols08232 rols08232ex18 rolsS127303 embryonic/larval pharynx rols08232 embryonic anterior Malpighian tubule rolsXX117 rolsEP3330 rols5a embryonic Malpighian tubule rolsXX117 rolsEP3330 rols5a embryonic myoblast rols08232 rolsA490.2M3 rolsAD328 rolsT192 rolsT321 rolsT59 rolsT627 rolsXX117 embryonic myoblast (with Df(3L)vin4) rolsT192 rolsT321 rolsT59 rolsT627 embryonic somatic muscle rols08232 rolsAD328 Malpighian tubule main body primordium rolsXX117 rolsEP3330 rols5a midgut constriction 1 rolsT627 muscle fiber | embryonic stage rolsT627 presumptive embryonic/larval digestive system rolsunspecified presumptive embryonic/larval muscle system (with Df(3L)BK9) rols08232ex18
Classical Alleles ( 30 )
For All Classical Alleles Show
Allele of rols Class Mutagen Stocks Known lesion rolsLA00796 P-element activity 2 Yes rols08232 P-element activity 1 Yes rolsA490.2M3 P-element activity 1 Yes rolsc05665 piggyBac transposase 1 -- rolsc07157 piggyBac transposase 1 -- rolsd04270 P-element activity 1 -- rolsf03014 piggyBac transposase 1 -- rolsf06187 1 -- rolsKG00312 P-element activity 1 -- rolsMI02479 1 -- rolsMI04487 1 -- rols08232ex18 P-element activity 0 Yes rols1027 P-element activity 0 -- rols1728-20L 0 -- rols5a Delta2-3 0 Yes rolsAD328 ethyl methanesulfonate 0 -- rolsc05442 0 -- rolsd04811 P-element activity 0 -- rolsEP3330 P-element activity 0 Yes rolsj2A6 P-element activity 0 -- rolsJTL1 P-element activity 0 -- rolsJTL2 P-element activity 0 -- rolsS127303 P-element activity 0 -- rolsT192 loss of function allele ethyl methanesulfonate 0 Yes rolsT321 loss of function allele ethyl methanesulfonate 0 Yes rolsT59 loss of function allele ethyl methanesulfonate 0 Yes rolsT627 loss of function allele ethyl methanesulfonate 0 Yes rolsunspecified 0 -- rolsX-1344 ethyl methanesulfonate 0 -- rolsXX117 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of rols Class Mutagen Stocks Known lesion rols300.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rols6.Scer\UAS in vitro construct - regulatory fusion 0 Yes rols7.Scer\UAS in vitro construct - regulatory fusion 0 Yes rolsAct5C.cMa in vitro construct - regulatory fusion 0 Yes rolsAct5C.T:Hsap\MYC,T:SV5\V5,T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rolsGD4036 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes rolsS241A.S243A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes rolsScer\UAS.C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rolsScer\UAS.N.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes rolsΔ1-1150.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1-137.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1-264.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1-439.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1-863.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1401-1900.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔ1739-1900.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes rolsΔC.Act5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)BK9 (Menon and Chia, 2001, Rau et al., 2001, Baumgartner et al., 1996, Massarwa et al., 2007) Df(3L)BSC395 (Christensen et al., 2007.10.29) Df(3L)BSC574 (Christensen et al., 2008.7.11) Df(3L)BSC840 (Cook et al., 2009.11.25) Df(3L)Exel6115 (Ryder, 2004.4.26) Df(3L)vin4 (Chen and Olson, 2001, Baumgartner et al., 1996) Df(3L)vin7 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(3L)vin8 (Baumgartner et al., 1996)
Not disrupted in	Df(3L)vin2 (Chen and Olson, 2001) Df(3L)vin3 (Chen and Olson, 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4036} NA P{UAS-rols.6} NA P{UAS-rols.7} NA P{UAS-rols.HA.C} NA P{UAS-rols.HA.N} NA P{UAS-rols.HA.Δ1-137} NA P{UAS-rols.HA.Δ1-264} NA P{UAS-rols.HA.Δ1-439} NA P{UAS-rols.HA.Δ1-863} NA P{UAS-rols.HA.Δ1-1150} NA P{UAS-rols.S241A.S243A} NA P{UAS-rols.Δ1401-1900.HA} NA P{UAS-rols.Δ1739-1900.HA} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}rolsj2A6 No P{lacW}rolsS127303 No P{lacZ}E(sda)HH No P{lacZ}rols1027 No P{lacZ}rolsJTL1 No P{lacZ}rolsJTL2 No P{lArB}rolsA490.2M3 No P{PZ}rols08232 No miscellaneous insertions Mi{MIC}rolsMI02479 NA Mi{MIC}rolsMI04487 NA P{SUPor-P}rolsKG00312 NA PBac{PB}rolsc05442 NA PBac{PB}rolsc05665 NA PBac{PB}rolsc07157 NA PBac{WH}rolsf03014 NA PBac{WH}rolsf06187 NA insertion of mobile activating element P{EP}rolsEP3330 NA P{GSV1}EP-297.2EP-297.2 NA P{Mae-UAS.6.11}rolsLA00796 NA P{XP}rolsd04270 NA P{XP}rolsd04811 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with kirre AND inferred from physical interaction with mbc (Chen and Olson, 2001)
Biological Process
CV term Evidence References
anterior Malpighian tubule development inferred from mutant phenotype (Puetz et al., 2005) border follicle cell migration inferred from mutant phenotype (Borghese et al., 2006) myoblast fusion inferred from mutant phenotype (Rau et al., 2001, Menon and Chia, 2001, Chen and Olson, 2001, Menon et al., 2005, Bulchand et al., 2010)
Cellular Component
CV term Evidence References
adherens junction inferred from direct assay (Menon et al., 2005) cell surface inferred from direct assay (Menon et al., 2005) cytoplasm inferred from direct assay (Chen and Olson, 2002, Chen and Olson, 2001, Menon et al., 2005)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR001841 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
myoblast fusion non-traceable author statement (Menon, 2001.8.18) traceable author statement (Dworak and Sink, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions rols allele Gene References rols08232 peb (Wilk et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64728 DroID - A comprehensive database of gene and protein interactions. • FBgn0041096 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39368 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8WS24
Orthologs
OrthoDB Orthologs (33) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6KSQ44 Dipteran inclusive ortholog search EOG6QZ7TV Insect inclusive ortholog search EOG62NGV5 Arthropod inclusive ortholog search EOG6HMGR4 Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6KSQ44)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\rols     Drosophila simulans   Dsim\GD12752 Y   Drosophila sechellia   Dsec\GM24683 Y   Drosophila erecta   Dere\GG13859 Y   Drosophila yakuba   Dyak\GE20152 Y   Drosophila ananassae   Dana\GF10397 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16675 Y   Drosophila persimilis   Dper\GL10318 Y   Drosophila willistoni   Dwil\GK17221 Y   Drosophila virilis   Dvir\GJ11316 Y   Drosophila mojavensis   Dmoj\GI11633 Y   Drosophila grimshawi   Dgri\GH14649 Y
Orthologs in non-Drosophila Dipterans (EOG6QZ7TV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005908   Anopheles gambiae Malaria mosquito  Agam\AGAP004244   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006231
Orthologs in non-Dipteran Insects (EOG62NGV5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB51447   Apis mellifera Western honey bee  Amel\GB50593   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008126   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15234   Atta cephalotes Leafcutter ant  Acep\ACEP19205   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18069   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20262   Linepithema humile Argentine ant  Lhum\LH20397   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24312   Solenopsis invicta Red fire ant  Sinv\SINV15239   Acyrthosiphon pisum Pea aphid  Apim\ACYPI060796   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006885   Bombyx mori Silkmoth  Bmor\BGIBMGA011306   Pediculus humanus Human body louse  Phum\PHUM548250   Tribolium castaneum Red flour beetle  Tcas\TC002665
Orthologs in non-Insect Arthropods (EOG6HMGR4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_317573   Daphnia pulex Water flea  Dpux\JGI_V11_319726   Ixodes scapularis Deer tick  Isca\ISCW012963
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12752 Drosophila sechellia Dsec\GM24683 Drosophila erecta Dere\GG13859 Drosophila yakuba Dyak\GE20152 Drosophila ananassae Dana\GF10397 Drosophila pseudoobscura pseudoobscura Dpse\GA16675 Drosophila persimilis Dper\GL10318 Drosophila willistoni Dwil\GK17221 Drosophila virilis Dvir\GJ11316 Drosophila mojavensis Dmoj\GI11633 Drosophila grimshawi Dgri\GH14649
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	35988 y1 w*; Mi{MIC}rolsMI02479 37687 y1 w*; Mi{MIC}rolsMI04487 22194 y1 w*; P{Mae-UAS.6.11}rolsLA00796 11027 P{lArB}rolsA490.2M3 ry506/TM3, Sb1 11729 P{PZ}rols08232 ry506/TM3, ryRK Sb1 Ser1 13085 y1 w67c23; P{SUPor-P}rolsKG00312 ry506/TM3, Sb1 Ser1
Harvard	- P{XP}rolsd04270 - PBac{PB}rolsc05665 - PBac{PB}rolsc07157 - PBac{WH}rolsf03014 - PBac{WH}rolsf06187
Kyoto	120796 y* w*; P{Mae-UAS.6.11}rolsLA00796
Genomic Clones ( 2 )
	BACR05P17 BACR14K14 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 38 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH15583
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP02947 RH01576 RH02455 RH03972
Other clones	31135 EC051461 EC051854 EC051897 EC052461 EC052902 EC053001 EC053009 EC053888 EC054397 EC054404 EC056264 EK140146 GM14106 RH09483 RP004046184 RP004107950 RP004122213 RP004131486 RP004206930 RP004232102 RP004243420 RP004243553 RP004305025 RP004333071 RP004433191 RP004473992 RP004481649 RP004491480 RP004502193 RP004514317 RP004522275 SD03901
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0041096 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39368
Antibody Information
	monoclonal (Kesper et al., 2007) polyclonal (Menon and Chia, 2001)
Other Information
Discoverer
Etymology
	The gene is named "rolling pebbles" after the mutant phenotype; mutant embryos have a scattered distribution of unfused myoblasts. (Rau et al., 2001)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: rols anon-WO0257455.19 (FlyBase, 1996-) Source for merge of: rols CG12277 (FlyBase, 1996-) Source for merge of: rols CG17155 CG5679 (FlyBase, 1996-) Source for merge of: rols l(3)08232 (Rau et al., 2001)
Additional comments	Annotations CG12277, CG17155, CG5679 merged as CG32096 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) "CG17155" is a candidate gene for "E(sda)H". (Zhang et al., 2002) Source for merge of rols anon-WO0257455.19 was sequence comparison (date:051113). (FlyBase, 1996-) Source for merge of rols CG17155 CG5679 was sequence comparison (date:011124). (FlyBase, 1996-) Source for merge of rols CG12277 was sequence identity (date:011221). (FlyBase, 1996-)
Other Comments
	The ankyrin repeats of the rols protein are essential for kirre-dependent translocation, whereas the TPR/coiled-coil region functions after translocation. (Menon et al., 2005) rols acts during myoblast fusion to link the initial event of myoblast attraction with the downstream event of myotubule structural organisation. (Menon and Chia, 2001) rols mutants are defective in myoblast fusion. (Menon and Chia, 2001) Mutations in rols result in severe defects in myoblast fusion. (Rau et al., 2001) rols is involved in muscle fusion in the developing embryo. (Rau et al., 2000) The fusion process during muscle development is severely disrupted in mutant embryos. (Rau et al., 2000) Complementation groups l(3)68Fa, l(3)68Fb, l(3)68Fc, l(3)68Fd, l(3)68Fe, Nrx-IV, l(3)69Aa and l(3)69Ab isolated by Hoogwerf et al. (FBrf0048238) probably correspond to complementation groups l(3)68Fg, l(3)j2D3, l(3)68Fh, rols, l(3)68Fi, Nrx-IV, l(3)69Ah and l(3)69Ai isolated by Baumgartner at al, 1996, Cell 87: 1059--1068, but a one to one correspondence cannot be made as Hoogwerf mutations are apparently lost. (Bellen, 1997.1.14) Complementation group isolated during complementation analysis of Nrx-IV region. (Baumgartner et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_140285 NM_168487 NM_168488 NM_168489 NM_168490 NM_001274807 NM_001274808 NM_001274809 NM_001274810 RefSeq (Proteins) • NP_648542 NP_729778 NP_729779 NP_729780 NP_729781 NP_001261736 NP_001261737 NP_001261738 NP_001261739 DDBJ / EMBL / GenBank • AAF49967 AAF49968 AAF49970 AAL13617 AAL30170 AAL30171 AAL32442 AAL32443 AAL50557 AAL50558 AAN12250 AAN12251 AF386647 AF386648 AF411457 AF411458 AF440568 AF440569 AGB94429 AGB94430 AGB94431 AGB94432 AI292638 AW940595 AX482651 AY058388 CZ470166 CZ471295 CZ473824 CZ474147 CZ485569 DU708667 G01171 Entrez Gene - A searchable database of RefSeq genes. • 39368 UniProtKB/TrEMBL • Q8WS23 Q8WS24 Q8WTD1 Q8WTD2 Q9VTS6 Q9VTS8 Q9VTS9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Zinc finger, RING-type (IPR001841) Ankyrin repeat (IPR002110) Tetratricopeptide-like helical (IPR011990) Tetratricopeptide repeat-containing domain (IPR013026) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Ankyrin repeat-containing domain (IPR020683) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH15583
Linkouts
	BioGRID - A database of protein and genetic interactions • 64728 DroID - A comprehensive database of gene and protein interactions. • FBgn0041096 DRSC - Results from RNAi screens. • FBgn0041096 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041096 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG32096-RD FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0041096 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39368 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/rollpeb1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39368 modMine - Data generated by the modENCODE project. • FBgn0041096 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8WS24
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	anon-WO0257455.19   Ants (Chen and Olson, 2005, Chen and Olson, 2004, Chen et al., 2007, Kim et al., 2007, Dobi et al., 2011) ANTS (Donaldson, 2003, Chen et al., 2003, Chen and Olson, 2002, Menon et al., 2005) ants (Chen and Olson, 2001, Dworak and Sink, 2002, Taylor, 2002, Chen, 2001.10.29, Borghese et al., 2006, Hossain et al., 2005) ants/rols (Beckett and Baylies, 2007) CG5679   CG12277 (Molnar et al., 2006) CG17155 (Zhang et al., 2002, ) CG32096   l(3)68Fd (Baumgartner et al., 1996) l(3)08232 (Birchler et al., 2001, Spradling et al., 1999, Bellen, 1997.1.14, BDGP Project Members, 1994-1999, ) l(3)j2A6   Rol/Ants (Taylor, 2003) rols (Menon et al., 2005, Christensen et al., 2007.10.29, Puetz et al., 2005, Molnar et al., 2006, Christensen et al., 2008.7.11, Zeitouni et al., 2007, Kesper et al., 2007, Haralalka et al., 2011, Borghese et al., 2006, Massarwa et al., 2007, Richardson et al., 2007, Carrasco-Rando and Ruiz-Gómez, 2008, Estrada et al., 2007, Stute et al., 2006, Cook et al., 2009.11.25, Bulchand et al., 2010, Hossain et al., 2005, Hazelett et al., 2009, Avirneni-Vadlamudi et al., 2012) Rols (Menon et al., 2005, Bulchand et al., 2010, Dobi et al., 2011) Rols7 (Chen and Olson, 2005, Chen and Olson, 2004, Menon et al., 2005, Griemert et al., 2007, Chen et al., 2007, Kesper et al., 2007) rols\ants (Artero et al., 2003)
Name Synonym	Antisocial (Donaldson, 2003, Chen and Olson, 2002, Chen et al., 2007, Dobi et al., 2011) antisocial (Taylor, 2002, Chen, 2001.10.29, Menon et al., 2005, Borghese et al., 2006) rolling pebbles (Rau et al., 2000, Puetz et al., 2005, Avirneni-Vadlamudi et al., 2012) Rolling pebbles (Dobi et al., 2011, Bulchand et al., 2010) rolling pebbles 7 (Menon et al., 2005)
Secondary FlyBase IDs
	FBgn0010928 FBgn0011323 FBgn0036241 FBgn0036243 FBgn0036244 FBgn0052096 FBgn0063612 FBgn0063798
References ( 73 )
Generate a list of	All references relating to this gene ( 73 )
List References by type	Research paper  ( 29 ) Supplementary material  ( 3 ) Review  ( 8 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 9 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 2 ) Website  ( 1 ) Curated genome annotation  ( 1 ) Patent  ( 1 )
Recent research papers ( 4 )
	Avirneni-Vadlamudi et al., 2012, J. Clin. Invest. 122(1): 403--407 Drosophila and mammalian models uncover a role for the myoblast fusion gene TANC1 in rhabdomyosarcoma. [FBrf0217060] Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Dobi et al., 2011, Fly 5(2): 68--75 Characterization of early steps in muscle morphogenesis in a Drosophila primary culture system. [FBrf0213797] Haralalka et al., 2011, Development 138(8): 1551--1562 Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. [FBrf0213340] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...