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Gene Dmel\Vhl

General Information
Symbol	Dmel\Vhl	Species	D. melanogaster
Name	von Hippel-Lindau	Annotation symbol	CG13221
Feature type	protein_coding_gene	FlyBase ID	FBgn0041174
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	dVHL, d-VHL
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	47E5-47E6	Sequence location	2R:7,167,230..7,168,538 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene von Hippel-Lindau is referred to in FlyBase by the symbol Dmel\Vhl (CG13221, FBgn0041174). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; ubiquitin-protein ligase activity. There is experimental evidence that it is involved in the biological process: negative regulation of microtubule depolymerization; centrosome organization; establishment or maintenance of polarity of follicular epithelium; spindle assembly involved in mitosis; cytoskeleton organization; open tracheal system development; lumen formation, open tracheal system; epithelial cell migration, open tracheal system; protein ubiquitination. 12 alleles are reported. The phenotypes of these alleles are annotated with: organ system; late extended germ band embryo; multicellular structure; non-connected developing system; tracheal branch primordium; portion of tissue; tracheal system; dorsal trunk primordium; eo support cell; organelle; dorsal ectoderm derivative. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: von Hippel-Lindau disease tumor suppressor, beta domain; von Hippel-Lindau disease tumour suppressor, beta/alpha domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636856_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Vhl. Gene sequence location is 2R:7167230..7168538.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Transgenic Constructs P{UAS-Vhl.RNAi.M} P{UAS-Vhl.n2} P{UAS-Vhl.n1} Alleles Vhl[Scer\UAS.n2] Vhl[dsRNA.Scer\UAS.cMa] Vhl[Scer\UAS.n1] Controlled Vocabulary Terms trachea development
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47E5-47E6   Limits computationally determined from genome sequence between P{PZ}rM547 and P{lacW}Fppsk06103&P{lacW}Fppsk03514
Experimentally Determined Cytological Location
Cytogenetic map Notes References 47E1-47E3   (determined by in situ hybridisation)   (Adryan et al., 2000) 47E1-47E3     (Adryan et al., 2000) 47E1-47E3     (Adryan, 1999.10.18)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vhl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vhl-RA FBtr0088115  NM_080247   1309   178 Vhl-RB FBtr0332420  NM_001273956   946   178
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vhl-PA   FBpp0087216   21.2   178   10.46     NP_524986   AAF58684 Vhl-PB   FBpp0304693   21.2   178   10.46     NP_001260885   AGB93418
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • von Hippel-Lindau disease tumor suppressor, beta domain (IPR024053) von Hippel-Lindau disease tumour suppressor, beta/alpha domain (IPR022772)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF58684   AC006066   AF195836 AAF29377   AE013599 AGB93418   AY075555 AAL68362   BI566497   BI576627   BI588289   BI612299   BI631887   BT023867   CO318369   DQ138668 ABA86274   EC089702   EL870606   EL870609   GH941955   GH942160   GH946167   GH947083   GH952163   GH957563   GH963112   GH964428   GH966768   GH966884   GH968555   GH969810   GH972188   GH975773   GH976903   GH979314   GH979766   GH984470
UniProtKB/Swiss-Prot	Q9V3C1
UniProtKB/TrEMBL	Q2XYJ4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 15 -- 17   embryonic/larval dorsal trunk   (Adryan et al., 2000) embryonic stage 16 -- 17   embryonic/larval transverse connective   (Adryan et al., 2000) embryonic stage 17   embryonic/larval visceral branch   (Adryan et al., 2000)
Additional Descriptive Data
	Vhl transcript is not expressed in the tracheal system before embryonic stage 15. (Adryan et al., 2000)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
VCB complex inferred from direct assay (Aso et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    170.25 Larval Midgut    172.6 Larval Hindgut    137.1 Larval Malpighian Tubules    188.4 Larval Fat Body    117.5 Larval Salivary Gland    160.5 Larval Trachea    136.9 Larval Carcass    97.575 Adult Head    189.8 Adult Eye    265.975 Adult Brain    280 Adult Thoracic-Abdominal Ganglion    272.3 Adult Crop    284.2 Adult Midgut    191.8 Adult Hindgut    228.3 Adult Malpighian Tubules    129.4 Adult Fat Body    199 Adult Salivary Gland    163.2 Adult Heart    219.25 Adult VirginFemale Spermatheca    187.2 Adult InseminatedFemale Spermatheca    158.3 Adult Ovary    214.8 Adult Testis    14.6 Adult Male Accessory Gland    208.3 Adult Carcass    119.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    189.8   NA  Eye    265.975   NA  Brain    280   170.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    272.3   NA  Crop    284.2   172.6  Midgut    191.8   137.1  Hindgut    228.3   188.4  Malpighian Tubules    129.4   117.5  Fat Body    199   160.5  Salivary Gland    163.2   NA  Heart    219.25   136.9  Trachea    NA   NA  VirginFemale Spermatheca    187.2   NA  InseminatedFemale Spermatheca    158.3   NA  Ovary    214.8   NA  Testis    14.6   NA  Male Accessory Gland    208.3   97.575  Carcass    119.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    2 central nervous system, pupae P8    2 head, virgin 1-day female    2 head, virgin 4-day female    1 head, virgin 20-day female    3 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    2 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    1 fat body, pupae P8    7 carcass, larvae L3 wandering    1 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    3 ovary, mated 4-day female    2 testis, mated 4-day male    1 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    9 embryo 04-06hr    17 embryo 06-08hr    18 embryo 08-10hr    14 embryo 10-12hr    18 embryo 12-14hr    9 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    11 embryo 20-22hr    10 embryo 22-24hr    8 larva L1    9 larva L2    6 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    9 white prepupae new    9 white prepupae 12hr    9 white prepupae 24hr    10 pupae 2d postWPP    9 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    14 adult female 05day    10 adult female 30day    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    7 embryonic 1182-4H    3 embryonic GM2    6 embryonic Kc167    9 embryonic S1    5 embryonic S3    7 leg disc CME L1    4 wing disc CME-W2    5 wing disc ML-DmD8    3 wing disc ML-DmD9    2 wing disc ML-DmD16-c3    2 wing disc ML-DmD21    3 wing disc ML-DmD32    2 haltere disc ML-DmD17-c3    2 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    5 mixed discs ML-DmD4-c1    2 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    3 tumorous blood cells mbn2    6 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    0 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    4 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    3 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_31 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RH61560 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041174 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13221-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS.cMa lethal | embryonic stage | non-rescuable maternal effect | recessive Vhl1 lethal | first instar larval stage (with Df(2R)en-A) Vhl1 lethal | first instar larval stage | recessive Vhl1 lethal | larval stage | recessive Vhl1 lethal | pupal stage, with Scer\GAL4pnr-MD237 VhlGD7964 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 VhlGD7964 viable, with Scer\GAL4en.PU VhldsRNA.Scer\UAS.cMa viable, with Scer\GAL4GMR.PU VhldsRNA.Scer\UAS.cMa Sterility Allele female sterile (with Vhl1), with Scer\GAL4btl.PS VhlScer\UAS.T:Ivir\HA1 female sterile (with VhlScer\UAS.T:Ivir\HA1), with Scer\GAL4btl.PS Vhl1 Other Phenotypes Allele cell polarity defective | somatic clone Vhl1 planar polarity defective, with Scer\GAL4pnr-MD237 VhlGD7964 visible, with Scer\GAL4pnr-MD237 VhlGD7964 Phenotype manifest in Allele dorsal trunk primordium VhlcAa dorsal trunk primordium, with Vhla.cAa VhlcAa dorsal trunk primordium, with VhlcAa Vhla.cAa egg chamber (with Vhl1), with Scer\GAL4btl.PS VhlScer\UAS.T:Ivir\HA1 egg chamber (with VhlScer\UAS.T:Ivir\HA1), with Scer\GAL4btl.PS Vhl1 embryonic/larval dorsal branch, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS.cMa embryonic/larval dorsal trunk Vhl1 embryonic/larval dorsal trunk, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS.cMa VhldsRNA.Scer\UAS VhlScer\UAS.cHa embryonic/larval lateral trunk, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS.cMa embryonic/larval trachea, with Scer\GAL4Act.PU VhldsRNA.Scer\UAS.cMa embryonic/larval trachea, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS.cMa embryonic/larval tracheal system Vhl1 embryonic/larval tracheal system, with Scer\GAL4btl.PS VhldsRNA.Scer\UAS embryonic/larval tracheal system | maternal effect Vhl1 follicle cell (with Vhl1), with Scer\GAL4btl.PS VhlScer\UAS.T:Ivir\HA1 follicle cell (with VhlScer\UAS.T:Ivir\HA1), with Scer\GAL4btl.PS Vhl1 follicle cell | heat sensitive VhldsRNA.hs follicle cell | somatic clone Vhl1 lateral trunk primordium, with Vhla.cAa VhlcAa lateral trunk primordium, with VhlcAa Vhla.cAa microtubule (with Vhl1), with Scer\GAL4btl.PS VhlScer\UAS.T:Ivir\HA1 microtubule (with VhlScer\UAS.T:Ivir\HA1), with Scer\GAL4btl.PS Vhl1 microtubule | somatic clone Vhl1 nurse cell (with Vhl1), with Scer\GAL4btl.PS VhlScer\UAS.T:Ivir\HA1 nurse cell (with VhlScer\UAS.T:Ivir\HA1), with Scer\GAL4btl.PS Vhl1 presumptive embryonic/larval tracheal system VhlcAa presumptive embryonic/larval tracheal system, with Vhla.cAa VhlcAa presumptive embryonic/larval tracheal system, with VhlcAa Vhla.cAa tracheal primordium | ectopic, with Scer\GAL4Act.PU VhldsRNA.Scer\UAS.cMa transverse connective primordium, with Vhla.cAa VhlcAa transverse connective primordium, with VhlcAa Vhla.cAa trichogen cell, with Scer\GAL4pnr-MD237 VhlGD7964 visceral branch primordium, with Vhla.cAa VhlcAa visceral branch primordium, with VhlcAa Vhla.cAa
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Vhl Class Mutagen Stocks Known lesion Vhl1 amorphic allele - genetic evidence recombination gene targeting by homologous recombination 0 Yes
Alleles Carried on Transgenic Constructs ( 14 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vhl Class Mutagen Stocks Known lesion VhlGD7964 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Vhla.cAa in vitro construct - RNAi 0 Yes VhlcAa in vitro construct - RNAi in vitro construct 0 Yes VhldsRNA.hs in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes VhldsRNA.Scer\UAS.cMa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes VhldsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Vhlhs.PD in vitro construct - regulatory fusion 0 Yes VhlKK111257 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes VhlRQ.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes VhlScer\UAS.cHa in vitro construct - regulatory fusion 0 Yes VhlScer\UAS.n1 in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes VhlScer\UAS.n2 in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes VhlScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes VhlYH.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)en-A (Duchi et al., 2010) Df(2R)Exel6060 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-Vhl.D} NA P{hs-Vhl.RNAi} NA UAS construct P{GD7964} NA P{KK111257} NA P{UAS-Vhl.H} NA P{UAS-Vhl.HA} NA P{UAS-Vhl.n1} NA P{UAS-Vhl.n2} NA P{UAS-Vhl.RNAi.M} NA P{UAS-Vhl.RNAi} NA P{UAS-Vhl.RQ.HA} NA P{UAS-Vhl.YH} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with UniProtKB:Q7KRW1 (assigned by UniProtKB) (Gemmill et al., 2002) contributes_to ubiquitin-protein ligase activity inferred from direct assay (Aso et al., 2000)
Biological Process
CV term Evidence References
centrosome organization inferred from genetic interaction with mgr (Delgehyr et al., 2012) cytoskeleton organization inferred from direct assay (Dammai et al., 2002) epithelial cell migration, open tracheal system inferred from mutant phenotype (Hsouna et al., 2010) establishment or maintenance of polarity of follicular epithelium inferred from mutant phenotype (Duchi et al., 2010) lumen formation, open tracheal system inferred from mutant phenotype (Hsouna et al., 2010) negative regulation of microtubule depolymerization inferred from mutant phenotype (Duchi et al., 2010) open tracheal system development inferred from mutant phenotype (Adryan et al., 2000) protein ubiquitination inferred from direct assay (Aso et al., 2000) spindle assembly involved in mitosis inferred from genetic interaction with mgr (Delgehyr et al., 2012) trachea development inferred from mutant phenotype (Mortimer and Moberg, 2009)
Cellular Component
CV term Evidence References
VCB complex inferred from direct assay (Aso et al., 2000)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Vhl pull down, autoradiography (Delgehyr et al., 2012) Vhl - Cul-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vhl - Elongin-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vhl - Elongin-C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vhl - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vhl - Trc8 anti bait coimmunoprecipitation, anti tag western blot, pull down, western blot (Gemmill et al., 2002) Vhl - awd anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation (Hsouna et al., 2010) Vhl - hyd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vhl - mgr pull down, autoradiography (Delgehyr et al., 2012) Vhl - βTub56D pull down, autoradiography (Delgehyr et al., 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vhl allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 72721 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0041174 DroID - A comprehensive database of gene and protein interactions. • FBgn0041174
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6M6647 Dipteran inclusive ortholog search EOG6V6ZS1 Insect inclusive ortholog search EOG6B5N2Q Arthropod inclusive ortholog search EOG6GMSDJ Metazoa inclusive ortholog search EOG68SVP8
Orthologs in Drosophila Species (EOG6M6647)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Vhl     Drosophila simulans   Dsim\GD10785 Y   Drosophila sechellia   Dsec\GM21273 Y   Drosophila erecta   Dere\GG20185 Y   Drosophila yakuba   Dyak\GE12873 Y   Drosophila ananassae   Dana\GF12540 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA12133 Y   Drosophila persimilis   Dper\GL17723 Y   Drosophila willistoni   Dwil\GK21683 Y   Drosophila virilis   Dvir\GJ15074 Y   Drosophila mojavensis   Dmoj\GI19906 Y   Drosophila grimshawi   Dgri\GH19949 Y
Orthologs in non-Drosophila Dipterans (EOG6V6ZS1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001105   Anopheles gambiae Malaria mosquito  Agam\AGAP002496   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009992
Orthologs in non-Dipteran Insects (EOG6B5N2Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Vhl   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010073   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12061   Atta cephalotes Leafcutter ant  Acep\ACEP10130   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19123   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14299   Linepithema humile Argentine ant  Lhum\LH15918   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24532   Solenopsis invicta Red fire ant  Sinv\SINV18574   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004863   Bombyx mori Silkmoth  Bmor\BGIBMGA009155   Pediculus humanus Human body louse  Phum\PHUM227970   Tribolium castaneum Red flour beetle  Tcas\Vhl
Orthologs in non-Insect Arthropods (EOG6GMSDJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\cytb   Ixodes scapularis Deer tick  Isca\ISCW001298
Orthologs in non-Arthropod Metazoa (EOG68SVP8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\vhl-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014320gn   Danio rerio Zebrafish  Drer\vhl   Danio rerio Zebrafish  Drer\vhll   Xenopus tropicalis Western clawed frog  Xtro\vhl   Gallus gallus Domestic chicken  Ggal\VHL   Mus musculus House mouse  Mmus\Vhl   Rattus norvegicus Norway rat  Rnor\Vhl   Homo sapiens Human  Hsap\VHL
Human Orthologs (1)
Gene OMIM HGNC Hsap\VHL
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10785 Drosophila sechellia Dsec\GM21273 Drosophila erecta Dere\GG20185 Drosophila yakuba Dyak\GE12873 Drosophila ananassae Dana\GF12540 Drosophila pseudoobscura pseudoobscura Dpse\GA12133 Drosophila persimilis Dper\GL17723 Drosophila willistoni Dwil\GK21683 Drosophila virilis Dvir\GJ15074 Drosophila mojavensis Dmoj\GI19906 Drosophila grimshawi Dgri\GH19949
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v32163 w1118; P{GD7964}v32163 v32164 w1118; P{GD7964}v32164
Genomic Clones ( 2 )
	BACR09B12 BACR29G22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 29 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP02669 RH61560
Other clones	DPiM_RH61560
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RH29443 RH33226 RH36059 RH40726
Other clones	166825 EK271824 IP02670 IP02672 RP004055666 RP004147839 RP004198460 RP004213482 RP004223203 RP004246669 RP004252109 RP004259264 RP004261236 RP004307749 RP004334571 RP004401004 RP004436378 RP004452827 RP004474846 RP004476788 RP004503915 RP004518239
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0041174 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 53433
Antibody Information
	polyclonal (Arquier et al., 2006, Hsouna et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Vhl BcDNA:RH61560 (FlyBase, 1996-) Source for merge of: vhl CG13221 (FlyBase, 1996-)
Additional comments	Source for merge of vhl CG13221 was sequence comparison (date:000605). (FlyBase, 1996-) Source for merge of Vhl BcDNA:RH61560 was TrEMBL update (date:020807). (FlyBase, 1996-)
Other Comments
	10% of embryos treated with Vhl dsRNA (to induce RNAi) reveal a narrow gap in the denticle pattern along the ventral midline. (Gemmill et al., 2002) Vhl is required for halting cell migration and tube outgrowth at the end of the tracheal tube formation. (Adryan et al., 2000) Loss of function mutations in Vhl show ectopic branching and looping in the tracheal system accompanied by breakage in the main trunk. (Adryan et al., 2000) Reduction of Vhl activity can either disrupt or induce tracheal growth, depending on the nature of the tracheal branches. (Adryan et al., 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080247 NM_001273956 RefSeq (Proteins) • NP_524986 NP_001260885 DDBJ / EMBL / GenBank • AAF29377 AAF58684 AAL68362 ABA86274 AC006066 AF195836 AGB93418 AY075555 BI631887 DQ138668 Entrez Gene - A searchable database of RefSeq genes. • 53433 UniProtKB/Swiss-Prot • Q9V3C1 UniProtKB/TrEMBL • Q2XYJ4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • von Hippel-Lindau disease tumor suppressor, beta domain (IPR024053) von Hippel-Lindau disease tumour suppressor, beta/alpha domain (IPR022772) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RH61560
Linkouts
	BioGRID - A database of protein and genetic interactions • 72721 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0041174 DroID - A comprehensive database of gene and protein interactions. • FBgn0041174 DRSC - Results from RNAi screens. • FBgn0041174 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041174 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG13221-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0041174 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 53433 modMine - Data generated by the modENCODE project. • FBgn0041174
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	BcDNA:RH61560   CG13221 (Keleman et al., 2009.8.5) dmVHL (Aso et al., 2000) dVHL (Centanin et al., 2003, Mortimer and Moberg, 2007, Arquier et al., 2006, Hsouna et al., 2010) d-VHL (Lavenburg et al., 2001, Adryan et al., 2000, Duchi et al., 2007) d-vhl (Adryan et al., 2000) DVhl (Gemmill et al., 2002) Dvhl (Gemmill et al., 2002) VHL (Dammai et al., 2002, Johnson and Krasnow, 2000, Mortimer and Moberg, 2009, Bakal et al., 2008, Irisarri et al., 2009, Duchi et al., 2010) vhl (Delgehyr et al., 2012) Vhl (Slack et al., 2007, Doronkin et al., 2010, Micale et al., 2009)
Name Synonym	Von Hippel Lindau (Lavista-Llanos et al., 2003) von Hippel-Lindau (Johnson and Krasnow, 2000, Hsouna et al., 2010) von Hippel Lindau (Irisarri et al., 2009) von Hippel Lindau protein (Delgehyr et al., 2012)
Secondary FlyBase IDs
	FBgn0033606 FBgn0040067 FBgn0061373
References ( 38 )
Generate a list of	All references relating to this gene ( 38 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 10 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 4 ) Website  ( 1 )
Recent research papers ( 1 )
	Delgehyr et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(15): 5729--5734 Drosophila Mgr, a Prefoldin subunit cooperating with von Hippel Lindau to regulate tubulin stability. [FBrf0218047] Show all research papers ...