dAtx2, l(3)06490, Atx-2, Sca2, dSCA2
Gene model reviewed during 5.53
Homodimer (PubMed:28388438). In the circadian pacemaker neurons, core component of the Atx2-tyf activator complex composed of at least Atx2, tyf, pAbp, Lsm12a (PubMed:28388438). In the circadian pacemaker neurons, also a core component of the Atx2-Not1 repressor complex composed of at least Atx2, tyf, pAbp, me31B (PubMed:28388438). Interacts (via PAM2 motif) with pAbp (PubMed:28388438). Interacts (via N-terminus) with tyf independently of pAbp (PubMed:28388438). Forms a subcomplex composed of Atx2 and pAbP which can associate with the 5' cap of pre-mRNAs independently of tyf, Lsm12a or me31B (PubMed:28388438). Interacts with Lsm12a and me31B (PubMed:28388438).
The PAM2 motif, and therefore interaction with pAbp, is essential for binding to the 5' cap of pre-mRNAs.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Atx2 using the Feature Mapper tool.
An antibody directed against the N-terminal part of Atx2-PB detects protein at all stages confirming that the upstream ATG is used. Levels are significantly higher in embryos and first instar larvae than in second instar larvae, third instar larvae, pupae, and adults. An antibody that detects both forms detects similar levels at all stages. Atx2 protein levels are higher in extracts from ovaries than in imaginal disc, CNS, fat body, or testis extracts.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Atx2 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
Area matching Drosophila EST AI062939.