FlyBase Gene Report: Dmel\Rheb

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Gene: Dmel\Rheb

General Information

Symbol

Dmel\Rheb

Species

D. melanogaster

Name

Ras homolog enriched in brain

Annotation Symbol

CG1081

Feature Type

protein_coding_gene

FlyBase ID

FBgn0041191

Gene Model Status

Stock Availability

15 publicly available

Gene Summary

Ras homolog enriched in brain (Rheb) encodes a Ras homolog that is the target of the product of gig. It activates the protein kinase encoded by mTor, leading to enlarged cell size. [Date last reviewed: 2018-11-08] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

dRheb, Ras homologue enriched in brain

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:5,568,921..5,570,491 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:5568921..5570491

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (30 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables GTPase activity

inferred from direct assay

(Patel et al., 2003)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables GDP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

enables GTP binding

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225, InterPro:IPR020849

(InterPro Project Members, 2004-)

enables GTPase activity

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001806

(InterPro Project Members, 2004-)

Biological Process (25 terms)

Terms Based on Experimental Evidence (23 terms)

CV Term

Evidence

References

involved_in apical protein localization

inferred from mutant phenotype

(Francis and Ghabrial, 2015)

involved_in carbohydrate homeostasis

inferred from mutant phenotype

(Murillo-Maldonado et al., 2011)

involved_in cholesterol homeostasis

inferred from mutant phenotype

(Danielsen et al., 2016)

involved_in G1/S transition of mitotic cell cycle

inferred from mutant phenotype

(Patel et al., 2003)

involved_in insulin receptor signaling pathway

inferred from mutant phenotype

(Yang et al., 2006, Saucedo et al., 2003, Kozma and Thomas, 2002)

involved_in lipid homeostasis

inferred from mutant phenotype

(Murillo-Maldonado et al., 2011)

involved_in multicellular organism growth

inferred from mutant phenotype

(Murillo-Maldonado et al., 2011)

involved_in negative regulation of autophagy

inferred from mutant phenotype

(Zirin et al., 2013)

involved_in negative regulation of lipophagy

inferred from mutant phenotype

(Lee et al., 2018)

involved_in negative regulation of macroautophagy

inferred from mutant phenotype

(Juhász et al., 2008)

involved_in negative regulation of TORC2 signaling

inferred from mutant phenotype

(Yang et al., 2006)

inferred from direct assay

(Wang et al., 2012)

involved_in positive regulation of axon guidance

inferred from mutant phenotype

(Dimitroff et al., 2012)

involved_in positive regulation of cell growth

inferred from mutant phenotype

(Patel et al., 2003)

involved_in positive regulation of cell size

inferred from mutant phenotype

(Parisi et al., 2011, Sims et al., 2009, Hall et al., 2007, Saucedo et al., 2003)

involved_in positive regulation of macroautophagy

inferred from mutant phenotype

(Pircs et al., 2012)

involved_in positive regulation of neuroblast proliferation

inferred from mutant phenotype

(Gil-Ranedo et al., 2019)

inferred from genetic interaction with FLYBASE:Hsp83; FB:FBgn0001233

(Huang and Wang, 2018)

involved_in positive regulation of ribosome biogenesis

inferred from mutant phenotype

(Hall et al., 2007)

involved_in positive regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Dimitroff et al., 2012)

involved_in positive regulation of TORC1 signaling

inferred from mutant phenotype

(Kim et al., 2017, Dimitroff et al., 2012, Parisi et al., 2011, Yang et al., 2006)

inferred from direct assay

(Wang et al., 2012)

involved_in positive regulation of translation

inferred from mutant phenotype

(Hall et al., 2007)

involved_in response to starvation

inferred from expression pattern

(Saucedo et al., 2003)

involved_in terminal branching, open tracheal system

inferred from mutant phenotype

(Francis and Ghabrial, 2015)

involved_in vascular endothelial growth factor receptor signaling pathway

inferred from genetic interaction with FLYBASE:PRAS40; FB:FBgn0267824

(Tran et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in signal transduction

inferred from electronic annotation with InterPro:IPR020849

(InterPro Project Members, 2004-)

involved_in small GTPase-mediated signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN001529822

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR020849

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000631348

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

Insulin-like Receptor Signaling Pathway Core Components

Pvr Signaling Pathway Core Components

Protein Family (UniProt)

Belongs to the small GTPase superfamily. Rheb family. (Q9VND8)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

GTPase activity
RHEA 19669:

Summaries

Gene Snapshot

Ras homolog enriched in brain (Rheb) encodes a Ras homolog that is the target of the product of gig. It activates the protein kinase encoded by mTor, leading to enlarged cell size. [Date last reviewed: 2018-11-08]

Gene Group (FlyBase)

RAS GTPASES -: The Ras family are members of the Ras superfamily of small GTPases. Ras proteins act in signal transduction cascades, generally the activated GTPase recruits and activates downstream effectors. Members this family are typically membrane-associated via isoprenylation of a CaaX motif in the C-terminus. (Adapted from PMID:15731001).

Pathway (FlyBase)

Insulin-like Receptor Signaling Pathway Core Components -: The Insulin-like Receptor (IR) signaling pathway in Drosophila is initiated by the binding of an insulin-like peptides to the Insulin-like receptor (InR). (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989.)
Pvr Signaling Pathway Core Components -: PDGF/VEGF (Platelet-Derived Growth Factor/Vascular Endothelial Growth Factor)-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).

Protein Function (UniProtKB)

Binds GTP and exhibits intrinsic GTPase activity (By similarity). Activates the protein kinase activity of TORC1, and thereby plays a role in the regulation of apoptosis (PubMed:22493059). Stimulates the phosphorylation of S6K through activation of TORC1 signaling (PubMed:22493059). May also have a role in activating TORC2 signaling (PubMed:22493059).

(UniProt, Q9VND8)

Summary (Interactive Fly)

Ras superfamily of G-proteins - functions downstream of the tumor suppressors Tsc1 and Tsc2 (Gigas in Drosophila), with Tsc2 functioning as a GAP for Rheb - signals to the growth regulator known as Target of rapamycin (TOR)

Interactive Fly

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Rheb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VND8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0078693

1225

182

FBtr0078694

1059

182

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0078342

20.7

182

5.32

FBpp0078343

20.7

182

5.32

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

182 aa isoforms: Rheb-PA, Rheb-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9VND8

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rheb using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rheb_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.89

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

medullary zone of lymph gland primary lobe

(Dragojlovic-Munther and Martinez-Agosto, 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rheb in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rheb

View exonic expression by tissue for Dmel\Rheb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041191

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

117332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041191

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041191

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041191

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1081

Flygut - An atlas of the Drosophila adult midgut

FBgn0041191

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rheb

Transgenic constructs containing regulatory region of Rheb

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^byn-Gal4

Rheb^AV4

lethal | pupal stage (with Rheb^7A1)

Rheb^26.2

lethal | pupal stage (with Rheb^26.2)

Rheb^7A1

lethal | recessive

Rheb^LA01053

lethal - all die before end of larval stage (with Rheb^PΔ1)

Rheb^PΔ2

lethal - all die before end of larval stage (with Rheb^PΔ2)

Rheb^PΔ1

lethal - all die before end of larval stage | recessive

Rheb^AV4

lethal - all die before end of pupal stage (with Rheb^3M2)

Rheb^26.2

lethal - all die before end of pupal stage (with Rheb^26.2)

Rheb^3M2

lethal - all die before end of second instar larval stage | recessive

Rheb^AV4

partially lethal - majority die | larval stage (with Rheb^7A1)

Rheb^26.2

partially lethal - majority die | larval stage (with Rheb^26.2)

Rheb^7A1

Rheb^7A1

viable (with Rheb^2D1)

Rheb^7A1

viable (with Rheb^2G5)

Rheb^44.1

viable (with Rheb^3M2)

Rheb^44.1

viable (with Rheb^7A1)

viable (with Rheb^44.1)

Sterility

Allele

female sterile (with Rheb^7A1)

Rheb^44.1

female sterile (with Rheb^44.1)

Rheb^7A1

Other Phenotypes

Allele

abnormal cell death | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

Rheb^{EP50.084-loxP}

abnormal cell number | P-stage, with Scer\GAL4^Mef2.247

Rheb^HMS00923

abnormal cell number | P-stage, with Scer\GAL4^phtm.PO, Scer\GAL80^spok.1.45

Rheb^HMS00923

abnormal cell size, with Scer\GAL4^ap-md544

Rheb^UAS.cPa

abnormal cell size | somatic clone

Rheb^AV4

abnormal developmental rate

Rheb^AV4

abnormal developmental rate (with Rheb^PΔ1)

Rheb^PΔ2

abnormal developmental rate (with Rheb^PΔ2)

Rheb^PΔ1

abnormal developmental rate | larval stage, with Scer\GAL4^C7

Rheb^UAS.cUa

abnormal eclosion rhythm | dominant | drug conditional

Rheb^AV4

abnormal mitotic cell cycle, with Scer\GAL4^Act5C.PP

Rheb^UAS.cSa

abnormal mitotic cell cycle | adult stage, with Scer\GAL4^sca.PU

Rheb^UAS.cPa

abnormal neuroanatomy, with Scer\GAL4^ccb-796

Rheb^{EP50.084-loxP}

abnormal neuroanatomy | adult stage | somatic clone

Rheb^3M2

abnormal neuroanatomy | nutrition conditional, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

abnormal neuroanatomy | nutrition conditional, with Scer\GAL4^elav-C155

Rheb^UAS.cPa

abnormal neuroanatomy | third instar larval stage

Rheb^UAS.cSa

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^BG380

Rheb^AV4

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PLu

Rheb^AV4

abnormal neurophysiology, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

abnormal phototaxis | nutrition conditional, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

abnormal size | adult stage, with Scer\GAL4^en-e16E

Rheb^UAS.cPa

abnormal size | adult stage, with Scer\GAL4^GMR.PF

Rheb^UAS.cSa

abnormal size | adult stage, with Scer\GAL4^ptc.PU

Rheb^UAS.cUa

abnormal starvation stress response | adult stage, with Scer\GAL4^ppl.PP

Rheb^UAS.cUa

chemical sensitive, with Scer\GAL4^P2.4.Pdf

Rheb^UAS.cPa

decreased body size | adult stage (with Rheb^2D1)

Rheb^7A1

decreased body size | adult stage (with Rheb^2G5)

Rheb^44.1

decreased body size | adult stage (with Rheb^3M2)

Rheb^44.1

decreased body size | adult stage (with Rheb^7A1)

decreased body size | adult stage (with Rheb^44.1)

decreased body size | adult stage | recessive

Rheb^7A1

decreased body size | first instar larval stage | recessive

Rheb^AV4

decreased body size | larval stage (with Rheb^7A1)

Rheb^26.2

decreased body size | larval stage (with Rheb^26.2)

Rheb^7A1

decreased body size | pharate adult stage, with Scer\GAL4^C7

Rheb^UAS.cUa

decreased body size | pupal stage (with Rheb^7A1)

Rheb^26.2

decreased body size | pupal stage (with Rheb^26.2)

Rheb^7A1

decreased cell number | somatic clone

Rheb^2D1

decreased cell size | somatic clone

decreased cell size | third instar larval stage, with Scer\GAL4^AB1

Rheb^HMS00923

decreased size | larval stage, with Scer\GAL4^ci.PC

Rheb^HMS00923

decreased size | P-stage, with Scer\GAL4^Mef2.247

Rheb^HMS00923

decreased size | spermatogenesis (with Rheb^7A1)

Rheb^44.1

decreased size | spermatogenesis (with Rheb^44.1)

Rheb^7A1

increased body size, with Scer\GAL4^GMR.PF

Rheb^{EP50.084-loxP}

increased body size | larval stage, with Scer\GAL4^da.G32

Rheb^UAS.cPa

increased cell death, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

increased cell number | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

Rheb^UAS.cPa

increased cell size

Rheb^{EP50.084-loxP}

increased cell size, with Scer\GAL4^Cg.PA

Rheb^UAS.cUa

increased cell size, with Scer\GAL4^en-e16E

Rheb^UAS.cSa

increased cell size, with Scer\GAL4^GMR.PF

Rheb^UAS.cSa

increased cell size, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

increased cell size | adult stage, with Scer\GAL4^Su(H).GBE

Rheb^UAS.cPa

increased cell size | somatic clone, with Scer\GAL4^Act5C.PP

increased cell size | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

Rheb^{EP50.084-loxP}

increased occurrence of cell division | larval stage, with Scer\GAL4^nab

Rheb^UAS.cUa

increased size | spermatogenesis, with Scer\GAL4^VP16.bam

Rheb^UAS.cPa

long lived | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

Rheb^HMS00923

majority die during larval stage, with Scer\GAL4^Tub.PU

Rheb^{EP50.084-loxP}

some die during larval stage (with Rheb^PΔ1)

Rheb^PΔ2

some die during larval stage (with Rheb^PΔ2)

Rheb^PΔ1

some die during larval stage | recessive

Rheb^AV4

some die during pupal stage (with Rheb^3M2)

Rheb^26.2

some die during pupal stage (with Rheb^26.2)

Rheb^3M2

some die during second instar larval stage | recessive

Rheb^AV4

visible, with Scer\GAL4^en.PU

visible, with Scer\GAL4^GMR.PF

Rheb^UAS.cPa

visible, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

visible, with Scer\GAL4^ptc-559.1

Rheb^UAS.cSa

visible | adult stage | recessive

Rheb^7A1

Phenotype manifest in

Allele

abdominal dorsal adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

Rheb^UAS.cPa

abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

Rheb^UAS.cPa

adult brain, with Scer\GAL4^elav.PLu

Rheb^1.1.UAS

adult gut, with Scer\GAL4^Su(H).GBE

Rheb^UAS.cPa

adult midgut, with Scer\GAL4^hs.PB

Rheb^NIG.1081R

antenna, with Scer\GAL4^Act5C.PI

Rheb^AV4

autophagosome | :chemical conditional | nutrition conditional, with Scer\GAL4^Mef2.PR

Rheb^UAS.cPa

autophagosome | adult stage, with Scer\GAL4^hs.PB

Rheb^NIG.1081R

autophagosome | third instar larval stage, with Scer\GAL4^phtm.PO

Rheb^UAS.cPa

dendrite | adult stage | somatic clone

Rheb^3M2

distal medullary amacrine neuron Dm8 | adult stage | somatic clone

Rheb^3M2

embryonic/larval fat body | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PU

Rheb^{EP50.084-loxP}

embryonic/larval hindgut, with Scer\GAL4^byn-Gal4

Rheb^AV4

embryonic/larval neuromuscular junction, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^BG380

Rheb^AV4

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PLu

Rheb^AV4

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

Rheb^UAS.cSa

embryonic/larval salivary gland (with Rheb^3M2)

Rheb^26.2

embryonic/larval salivary gland (with Rheb^26.2)

Rheb^3M2

enteroblast | adult stage, with Scer\GAL4^Su(H).GBE

Rheb^UAS.cPa

enteroendocrine cell | P-stage | decreased number, with Scer\GAL4^Mef2.247

Rheb^HMS00923

enteroendocrine cell | P-stage | decreased number, with Scer\GAL4^phtm.PO, Scer\GAL80^spok.1.45

Rheb^HMS00923

eye, with Scer\GAL4^Act5C.PI

Rheb^AV4

eye, with Scer\GAL4^GMR.PF

eye, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

eye | somatic clone

fat body | conditional | somatic clone, with Scer\GAL4^GMR.PF

Rheb^UAS.cSa

fat body | somatic clone, with Scer\GAL4^Act5C.PP

Rheb^EP50.084

fat cell | third instar larval stage, with Scer\GAL4^AB1

Rheb^HMS00923

first posterior wing cell, with Scer\GAL4^ptc-559.1

Rheb^UAS.cSa

head, with Scer\GAL4^Act5C.PI

Rheb^AV4

head | somatic clone

Rheb^AV4

imaginal disc (with Rheb^3M2)

Rheb^26.2

imaginal disc (with Rheb^26.2)

Rheb^3M2

imaginal disc anterior compartment, with Scer\GAL4^ci.PC

Rheb^HMS00923

interommatidial bristle | increased number, with Scer\GAL4^Act5C.PI

Rheb^AV4

intestinal stem cell | temperature conditional, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

Rheb^UAS.cPa

larval fat cell | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

Rheb^{EP50.084-loxP}

larval midgut enterocyte | temperature conditional, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

Rheb^UAS.cPa

larval neuroblast, with Scer\GAL4^nab

Rheb^UAS.cUa

lipid droplet | adult stage, with Scer\GAL4^hs.PB

Rheb^NIG.1081R

mesothoracic bristle | adult stage | increased number, with Scer\GAL4^sca.PU

Rheb^UAS.cPa

mitotic cell cycle, with Scer\GAL4^Act5C.PP

Rheb^UAS.cSa

muscle system | P-stage, with Scer\GAL4^Mef2.247

Rheb^HMS00923

neuroblast | third instar larval stage | nutrition conditional, with Scer\GAL4^insc-Mz1407

Rheb^UAS.cPa

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

Rheb^UAS.cSa

nucleolus, with Scer\GAL4^en-e16E

Rheb^UAS.cSa

ommatidium, with Scer\GAL4^Act5C.PI

Rheb^AV4

ommatidium, with Scer\GAL4^GMR.PF

Rheb^EP50.084

ommatidium, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

ommatidium (with Rheb^2G5)

Rheb^44.1

ommatidium (with Rheb^3M2)

Rheb^44.1

ommatidium (with Rheb^7A1)

Rheb^44.1

ommatidium (with Rheb^44.1)

ommatidium | somatic clone

ovary (with Rheb^7A1)

Rheb^44.1

ovary (with Rheb^44.1)

Rheb^7A1

photoreceptor cell R1 | somatic clone

Rheb^2D1

photoreceptor cell R6 | somatic clone

Rheb^2D1

photoreceptor cell R7 | nutrition conditional, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

photoreceptor cell R7 | nutrition conditional, with Scer\GAL4^elav-C155

Rheb^UAS.cPa

photoreceptor cell R8 | nutrition conditional, with Scer\GAL4^elav.PLu

Rheb^UAS.cPa

photoreceptor cell R8 | nutrition conditional, with Scer\GAL4^elav-C155

Rheb^UAS.cPa

photoreceptor neuron, with Scer\GAL4^GMR.PF

Rheb^UAS.cSa

photoreceptor neuron, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

rhabdomere, with Scer\GAL4^GMR.PU

Rheb^UAS.cSa

salivary gland | conditional | somatic clone, with Scer\GAL4^GMR.PF

Rheb^UAS.cSa

spermatocyte, with Scer\GAL4^VP16.bam

Rheb^UAS.cPa

spermatocyte (with Rheb^7A1)

Rheb^44.1

spermatocyte (with Rheb^44.1)

Rheb^7A1

synapse, with Scer\GAL4^ccb-796

Rheb^{EP50.084-loxP}

terminal tracheal cell, with Scer\GAL4^bs.PM

Rheb^HMS00923

Rheb^7A1

wing (with Rheb^3M2)

Rheb^44.1

wing (with Rheb^7A1)

Rheb^44.1

wing (with Rheb^44.1)

wing blade, with Scer\GAL4^ptc.PU

Rheb^UAS.cUa

wing blade anterior compartment, with Scer\GAL4^ap-md544

Rheb^UAS.cPa

wing blade dorsal compartment, with Scer\GAL4^ap-md544

Rheb^UAS.cPa

wing blade posterior compartment, with Scer\GAL4^ap-md544

Rheb^UAS.cPa

wing blade posterior compartment, with Scer\GAL4^en.PU

wing disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PU

Rheb^{EP50.084-loxP}

wing posterior compartment, with Scer\GAL4^en-e16E

Rheb^UAS.cPa

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (67)

11 of 14

Yes

Yes

1

6 of 14

No

Yes

2 of 14

No

No

1

2 of 14

No

No

1

2 of 14

No

No

2 of 14

No

No

3

2 of 14

No

No

2

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

1

2 of 14

No

No

3

2 of 14

No

No

3

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

1

2 of 14

No

No

1

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1

2 of 14

No

No

2

2 of 14

No

No

1 of 14

No

No

0

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

5

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1

1 of 14

No

No

7

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (41)

11 of 14

Yes

Yes

5 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (42)

10 of 14

Yes

Yes

6 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (48)

6 of 13

Yes

Yes

5 of 13

No

Yes

5 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xtro\LOC100488596

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (56)

13 of 14

Yes

Yes

3 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

Drer\LOC100332969

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

Drer\si:dkeyp-59c12.1

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

Drer\zgc:110699

2 of 14

No

No

Drer\zgc:171704

2 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Drer\si:dkey-27j5.5

1 of 14

No

No

Drer\zgc:100918

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (36)

11 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

Anopheles gambiae (African malaria mosquito) (30)

Agam\AgaP_AGAP001902

11 of 12

Yes

Yes

Agam\AgaP_AGAP002219

2 of 12

No

No

Agam\AgaP_AGAP002563

2 of 12

No

No

Agam\AgaP_AGAP002812

2 of 12

No

No

Agam\AgaP_AGAP003577

2 of 12

No

No

Agam\AgaP_AGAP004769

2 of 12

No

No

Agam\AgaP_AGAP005159

2 of 12

No

No

Agam\AgaP_AGAP005243

2 of 12

No

No

Agam\AgaP_AGAP005248

2 of 12

No

No

Agam\AgaP_AGAP005302

2 of 12

No

No

Agam\AgaP_AGAP006025

2 of 12

No

No

Agam\AgaP_AGAP007369

2 of 12

No

No

Agam\AgaP_AGAP007615

2 of 12

No

No

Agam\AgaP_AGAP007681

2 of 12

No

No

Agam\AgaP_AGAP008224

2 of 12

No

No

Agam\AgaP_AGAP008989

2 of 12

No

No

Agam\AgaP_AGAP011306

2 of 12

No

No

Agam\AgaP_AGAP012108

2 of 12

No

No

Agam\AgaP_AGAP001211

1 of 12

No

No

Agam\AgaP_AGAP001573

1 of 12

No

No

Agam\AgaP_AGAP001617

1 of 12

No

No

Agam\AgaP_AGAP001874

1 of 12

No

No

Agam\AgaP_AGAP003028

1 of 12

No

No

Agam\AgaP_AGAP004146

1 of 12

No

No

Agam\AgaP_AGAP005255

1 of 12

No

No

Agam\AgaP_AGAP006279

1 of 12

No

No

Agam\AgaP_AGAP010875

1 of 12

No

No

Agam\AgaP_AGAP011283

1 of 12

No

No

Agam\AgaP_AGAP011363

1 of 12

No

No

Agam\CDC42_ANOGA

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (32)

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

1 of 13

Yes

No

Saccharomyces cerevisiae (Brewer's yeast) (10)

9 of 13

Yes

Yes

2 of 13

No

No

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (3)

10 of 12

Yes

Yes

2 of 12

No

No

1 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rheb. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

insulin pathway effects, RHEB-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 5 )

Allele

Disease

Evidence

References

model of type 2 diabetes mellitus

CEA with Rheb^7A1

(Murillo-Maldonado et al., 2011)

CEA with Rheb^44.1

(Murillo-Maldonado et al., 2011)

model of type 2 diabetes mellitus

CEA with Rheb^7A1

(Murillo-Maldonado et al., 2011)

CEA with Rheb^2D1

(Murillo-Maldonado et al., 2011)

model of type 2 diabetes mellitus

CEA

(Murillo-Maldonado et al., 2011)

CEA with Rheb^2D1

(Murillo-Maldonado et al., 2011)

CEA with Rheb^44.1

(Murillo-Maldonado et al., 2011)

Rheb^{EP50.084-loxP}

model of disease of metabolism

CEA

(Devilliers et al., 2021)

model of tuberous sclerosis

CEA

(Dimitroff et al., 2012)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

exacerbates syndrome

modeled by RpS23^R67K

(Recasens-Alvarez et al., 2021)

exacerbates tauopathy

modeled by Hsap\MAPT^{S202A.T205A.UAS}

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{T231A.S235A.UAS}

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{S396A.S404A.UAS}

(Steinhilb et al., 2007)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Steinhilb et al., 2007)

Rheb^lexAop.cRa

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Jarabo et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RHEB; Ras homolog, mTORC1 binding

11 of 14

RHEBL1; RHEB like 1

6 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

On

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rheb - 14-3-3ε

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot, Identification by mass spectrometry

(Le et al., 2016, Vinayagam et al., 2016)

Rheb - 14-3-3ζ

anti tag coimmunoprecipitation, anti tag western blot, pull down, western blot

(Le et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rheb - ATPsynβ

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Kim and Choi, 2019, Vinayagam et al., 2016)

anti bait coimmunoprecipitation, western blot, pull down, anti tag coimmunoprecipitation, anti tag western blot

(Kim and Choi, 2019)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

bimolecular fluorescence complementation, fluorescence microscopy

(Känel et al., 2022)

anti tag coimmunoprecipitation, anti tag western blot

(Connolly et al., 2019)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, western blot, pull down, anti tag western blot

(Frappaolo et al., 2022, Le et al., 2016, Hsu et al., 2007)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, peptide massfingerprinting, enzymatic study, autoradiography

(Glatter et al., 2011, Zhang et al., 2003)

Rheb - l(2)09851

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rheb - qkr58E-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, western blot

(Frappaolo et al., 2022)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Dimitroff et al., 2012)

enhanceable

(Dimitroff et al., 2012)

suppressible

(Wang et al., 2009)

suppressible

(Khurana et al., 2006)

suppressible

(Li et al., 2017)

suppressible

(Reis and Edgar, 2004)

suppressible

(Pathak and Varghese, 2021)

suppressible

(Dimitroff et al., 2012, Stocker et al., 2003)

suppressible

(Liu et al., 2017)

enhanceable

(Devilliers et al., 2021)

suppressible

(Stocker et al., 2003)

enhanceable

(Kim et al., 2008)

suppressible

(Dimitroff et al., 2012)

suppressible

(Khurana et al., 2006)

suppressible

(Dimitroff et al., 2012)

suppressible

(Dimitroff et al., 2012, Saucedo et al., 2003, Stocker et al., 2003)

suppressible

(Stocker et al., 2003)

suppressible

(Lee et al., 2010)

suppressible

(Dimitroff et al., 2012)

suppressible

(Stocker et al., 2003)

suppressible

(Avet-Rochex et al., 2014)

suppressible

(Wong et al., 2015)

suppressible

(Rojas Villa et al., 2019)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Scott et al., 2007)

suppressible

(Zhang et al., 2014)

enhanceable

(Kim and Choi, 2019)

suppressible

(Pathak and Varghese, 2021)

suppressible

(Rabinovich et al., 2016)

enhanceable

(Goberdhan et al., 2005)

suppressible

(Yaniv et al., 2012)

enhanceable

(Karbowniczek et al., 2010)

enhanceable

(Wong et al., 2013)

suppressible

(Redhai et al., 2020)

suppressible

(Saucedo et al., 2003)

Pi3K92E|btl::Egfr

suppressible

(Jarabo et al., 2021)

suppressible

(Kamalesh et al., 2017)

suppressible

(Pallares-Cartes et al., 2012)

suppressible

(Mensah et al., 2015)

enhanceable

(Devilliers et al., 2021)

suppressible

(Cong et al., 2021, Willecke et al., 2011)

suppressible

(Hsieh et al., 2010)

suppressible

(Hsieh et al., 2010)

suppressible

(Recasens-Alvarez et al., 2021)

enhanceable

(Recasens-Alvarez et al., 2021)

suppressible

(Wong et al., 2012)

suppressible

(Stocker et al., 2003, Zhang et al., 2003)

suppressible

(Saucedo et al., 2003)

suppressible

(Avet-Rochex et al., 2014)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0041191

MIST (genetic) - An integrated Molecular Interaction Database

117332

MIST (protein-protein) - An integrated Molecular Interaction Database

117332

Pathways

Signaling Pathways (FlyBase)

Insulin-like Receptor Signaling Pathway Core Components
Pvr Signaling Pathway Core Components

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-47.5

Cytogenetic map

Sequence location

3R:5,568,921..5,570,491 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83B2-83B2

Limits computationally determined from genome sequence between P{PZ}Snr1⁰¹³¹⁹&P{PZ}Itp-r83A⁰⁵⁶¹⁶ and P{lacW}Atu^s1938

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

9688

w^*; P{UAS-Rheb.Pa}2

9689

w^*; P{UAS-Rheb.Pa}3

80966

y¹ w^*; P{hs-Rheb}9

80932

w^*; P{UAS-Rheb.S}13.1; P{UAS-mTor.WT}III

33966

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00923}attP2

9690

y¹ w^*; P{Mae-UAS.6.11}Rheb^AV4/TM6B, Tb¹

24479

P{PYD2+}1c, w^*; Adc^b-1; Df(3R)noi-B, e¹/TM3, Sb¹

121053

y^* w^*; P{Mae-UAS.6.11}Rheb^LA01053

1081R-2

1081R-4

HMJ21217

y¹ v¹; P{TRiP.HMJ21217}attP40/CyO

HMS00923

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00923}attP2

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (81)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH10361

Other clones

DPiM_GH10361

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0041191

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0041191

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Le et al., 2016, Findlay et al., 2007)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA made from templates generated with primers directed against this gene results in a reduced mean cell diameter. Silencing Rheb generates a cell-expression profile similar to that of rapamycin-treated cells.

(Guertin et al., 2006)

dsRNA made from templates generated with primers directed against this gene used to treat S2 cells.

(Yang et al., 2006)

dsRNA made from templates generated with primers directed against this gene promotes the phosphorylation of Akt1 (dependent on TORC2) and inhibits phosphorylation of S6k in S2 cells. In addition, knockdown of Rheb enhances insulin-stimulated Akt1 phosphorylation.

(Yang et al., 2006)

Signaling through Tor and its upstream regulators Pi3K92E/Pi3K21B and Rheb is necessary and sufficient to suppress starvation-induced autophagy in the fat body.

(Scott et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Levels of Rheb mRNA are rapidly induced in response to protein starvation.

(Saucedo et al., 2003)

In mitotic tissues, overexpression of Rheb accelerates passage through G1-S phase without affecting rates of cell division, whereas in endoreplicating tissues, overexpression increases DNA ploidy. Overexpression of Rheb can also drive cell growth in starved animals.

(Saucedo et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Rheb CG1081

(Saucedo and Edgar, 2002)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Named "Rheb" after the human ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG1081

(National Institute of Genetics Fly Stocks, 2023-, Çiçek et al., 2016, Fukuoh et al., 2014, Glatter et al., 2011, Ni et al., 2010.12.1, Goshima et al., 2007, Walker et al., 2006, Colicelli, 2004, Schneider et al., 2004, Wheeler et al., 2004, Saucedo et al., 2003, Stocker et al., 2003, Saucedo, 2002.10.25)

DReb

(Patel et al., 2003)

Dm Rheb

(Jekely, 2003)

DmRheb

(Urano et al., 2000)

Q9VND8

(Rojas et al., 2012)

RHEB

(Känel et al., 2022, Øvrebø et al., 2022, Cox et al., 2021, Ahmad et al., 2018, Lee et al., 2018, Androschuk et al., 2015, Koyama et al., 2013, Papatheodorou et al., 2012, Taillebourg et al., 2012, Oldham, 2011, Edgar, 2006)

RheB

(Ramanathan et al., 2019, Hou et al., 2008, Goshima et al., 2007, Goshima et al., 2007, Hoshizaki and Gibbs, 2007)

Rheb

(Jeong, 2024, Kosakamoto et al., 2024, Liu and He, 2024, Rodríguez-Vázquez et al., 2024, Turingan et al., 2024, Wang et al., 2024, Zhang et al., 2024, Heigwer et al., 2023, Nagai et al., 2023, Chang et al., 2022, Deliu et al., 2022, Francis et al., 2022, Gao et al., 2022, Jarabo et al., 2022, Kakanj et al., 2022, Liu et al., 2022, Miao et al., 2022, Thangadurai et al., 2022, Azuma et al., 2021, Bilder et al., 2021, Bonfini et al., 2021, Cho et al., 2021, Cong et al., 2021, Heier et al., 2021, Hou and Pei, 2021, Joy et al., 2021, Kim and O'Connor, 2021, Recasens-Alvarez et al., 2021, Yang et al., 2021, Blanco et al., 2020, Ghosh et al., 2020, Lee et al., 2020, Liu et al., 2020, Luo et al., 2020, Luo et al., 2020, Mariano et al., 2020, Murakawa et al., 2020, Parniewska and Stocker, 2020, Redhai et al., 2020, Robles-Murguia et al., 2020, Rodriguez-Fernandez et al., 2020, Rust et al., 2020, Texada et al., 2020, Zhao et al., 2020, Connolly et al., 2019, Galenza and Foley, 2019, Kim and Choi, 2019, Lee et al., 2019, Li et al., 2019, Sharma et al., 2019, Zheng, 2019.3.1, Aboudhiaf et al., 2018, Ayala et al., 2018, Huang and Wang, 2018, Inoue et al., 2018, Nowak et al., 2018, Staats et al., 2018, Tang et al., 2018, Zeng et al., 2018, Jonchère et al., 2017, Kamalesh et al., 2017, Keesey et al., 2017, Li et al., 2017, Liu and Jin, 2017, Liu et al., 2017, Melani et al., 2017, Mensah et al., 2017, Murillo-Maldonado and Riesgo-Escovar, 2017, Romero-Pozuelo et al., 2017, Wen et al., 2017, Danielsen et al., 2016, David-Morrison et al., 2016, Dobson et al., 2016, Etchegaray et al., 2016, Koyama and Mirth, 2016, Kuleesha et al., 2016, Le et al., 2016, Mukherjee et al., 2016, Parkhitko et al., 2016, Rabinovich et al., 2016, Vinayagam et al., 2016, Aradhya et al., 2015, Aradska et al., 2015, Das and Dobens, 2015, Francis and Ghabrial, 2015, Grotewiel and Bettinger, 2015, Kuhn et al., 2015, Lin et al., 2015, Mensah et al., 2015, Nässel et al., 2015, Nie et al., 2015, Rojas-Benitez et al., 2015, Takáts et al., 2015, Van Bortle et al., 2015, Zhang and Baehrecke, 2015, Avet-Rochex et al., 2014, Barrio et al., 2014, Deivasigamani et al., 2014, DeVorkin et al., 2014, Frost et al., 2014, Ghosh et al., 2014, Homem et al., 2014, Mulakkal et al., 2014, Zhang et al., 2014, Gordon et al., 2013, Ibar et al., 2013, Luo et al., 2013, Natarajan et al., 2013, Nowak et al., 2013, O'Farrell et al., 2013, Parisi et al., 2013, Quan et al., 2013, Shim et al., 2013, Shin et al., 2013, Sopko and Perrimon, 2013, Tran et al., 2013, Wong et al., 2013, Zirin et al., 2013, Alvarez-Ponce et al., 2012, Avet-Rochex et al., 2012, Dimitroff et al., 2012, Dragojlovic-Munther and Martinez-Agosto, 2012, Japanese National Institute of Genetics, 2012.5.21, Kapuria et al., 2012, Kaun et al., 2012, Lee et al., 2012, Mirth and Shingleton, 2012, Ogmundsdóttir et al., 2012, Pallares-Cartes et al., 2012, Pircs et al., 2012, Tokusumi et al., 2012, Wong et al., 2012, Yaniv et al., 2012, Zitserman et al., 2012, Barth et al., 2011, Diez et al., 2011, Eddison et al., 2011, Glatter et al., 2011, Lindquist et al., 2011, Murillo-Maldonado et al., 2011, Sousa-Nunes et al., 2011, Tang et al., 2011, Willecke et al., 2011, Chang and Neufeld, 2010, Daubert et al., 2010, Haberman et al., 2010, Karbowniczek et al., 2010, Kockel et al., 2010, Kong et al., 2010, Lee et al., 2010, Li et al., 2010, Li et al., 2010, Nisoli et al., 2010, Ribeiro and Dickson, 2010, Zheng and Sehgal, 2010, Chang and Neufeld, 2009, Chang and Neufeld, 2009, Doumanis et al., 2009, Nuzhdin et al., 2009, Parrish et al., 2009, Patch et al., 2009, Sims et al., 2009, Goraksha and Neufeld, 2008, Harvey et al., 2008, Juhász et al., 2008, Kim et al., 2008, Lee et al., 2008, McNeill et al., 2008, Rehmann et al., 2008, Bakal et al., 2007, Curtis et al., 2007, Findlay et al., 2007, Grewal et al., 2007, Hall et al., 2007, Hsu and Choi, 2007, Knox et al., 2007, Lee et al., 2007, Scott et al., 2007, Scott et al., 2007, Steinhilb et al., 2007, Tseng et al., 2007, Friedman and Perrimon, 2006, Hennig et al., 2006, Herranz et al., 2006, Khurana et al., 2006, Martin-Pena et al., 2006, Patel and Tamanoi, 2006, Walker et al., 2006, Wu and Brown, 2006, Wullschleger, 2006, Yang et al., 2006, Goberdhan et al., 2005, Liebl and Featherstone, 2005, Aspuria and Tamanoi, 2004, Reis and Edgar, 2004, Wheeler et al., 2004)

dRheb

(Frappaolo and Giansanti, 2023, Álvarez-Rendón et al., 2018, Gupta et al., 2013, Katewa and Kapahi, 2011, Murillo-Maldonado et al., 2011, Dekanty et al., 2010, Layalle et al., 2008, Hsu et al., 2007, Guertin et al., 2006, Inoki and Guan, 2006, Mosimann et al., 2006, Yang et al., 2006, Dekanty et al., 2005, Findlay et al., 2005, Goraksha and Neufeld, 2005, Patel et al., 2003, Stocker et al., 2003)

Name Synonyms

RAS homolog enriched in brain

(Ghosh et al., 2014)

Ras homolog enriched in brain

(Kaun et al., 2012, Ribeiro and Dickson, 2010, Hall et al., 2007)

Ras homolog enriched in brain ortholog

Ras homologue enriched in brain

(Wong et al., 2013, Wang et al., 2009)

Ras homology enriched in brain

(Yang et al., 2006)

Rheb

(Patch et al., 2009)

Secondary FlyBase IDs

FBgn0037343

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

PPI_InR-Tor_interaction_map

interactome

Interaction map generated by purification of insulin pathway factors, with identification of copurifying proteins by mass spectrometry.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

117332

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VND8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VND8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VND8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041191

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

117332/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041191

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041191

FlyMine - An integrated database for Drosophila genomics

FBgn0041191

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1081

MARRVEL_MODEL - MARRVEL (model organism gene)

117332

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0041191

DroID - A comprehensive database of gene and protein interactions.

FBgn0041191

DRSC - Results frm RNAi screens

FBgn0041191

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rheb_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041191

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0041191

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1081

Flygut - An atlas of the Drosophila adult midgut

FBgn0041191

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

117332

MIST (protein-protein) - An integrated Molecular Interaction Database

117332

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (356)