Gene Dmel\Prat2

FlyGene Wiki Help Close All Open All

General Information

Symbol

Dmel\Prat2

Species

D. melanogaster

Name

Phosphoribosylamidotransferase 2

Annotation symbol

CG10078

Feature type

protein_coding_gene

FlyBase ID

FBgn0041194

Gene Model Status

Current

Stock availability

2 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

65D2-65D3

Sequence location

3L:6,905,099..6,907,976 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Phosphoribosylamidotransferase 2 is referred to in FlyBase by the symbol Dmel\Prat2 (CG10078, FBgn0041194). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: amidophosphoribosyltransferase activity. Based on sequence similarity, it is predicted to be involved in the biological process: 'de novo' IMP biosynthetic process. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Amidophosphoribosyl transferase; Class II glutamine amidotransferase domain; Glutamine amidotransferase type 2 domain; Phosphoribosyltransferase domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye. Comments on Affy2 ProbeSet: ProbeSet 1624586_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Prat2. Gene sequence location is 3L:6905099..6907976.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

Update Feed

Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.

FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65D2-65D3

Limits computationally determined from genome sequence between P{PZ}l(3)02094⁰²⁰⁹⁴ and P{lacW}l(3)L4060^L4060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Prat2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Prat2-RA

FBtr0076946

NM_168181

1737

470

Prat2-RB

FBtr0076945

NM_079225

1932

547

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Prat2-PA

FBpp0076655

51.3

470

7.18

NP_729191

AAF50639

Prat2-PB

FBpp0076654

59.5

547

6.88

NP_523949

AAF50638

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Class II glutamine amidotransferase domain (IPR000583)

Phosphoribosyltransferase domain (IPR000836)

Amidophosphoribosyl transferase (IPR005854)

Glutamine amidotransferase type 2 domain (IPR017932)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

yolk nucleus

(Fisher et al., 2012)

embryonic stage 11 -- 12

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

yolk nucleus

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 17

organism

(Malmanche et al., 2003)

second instar larval stage -- day 5 of adulthood

organism

(Malmanche et al., 2003)

Additional Descriptive Data

Prat2 transcript is expressed from 4 hours AEL during embryogenesis; no maternal component is detected. Expression levels are highest from 4 through 8 hours AEL, then drop sharply from 16-20 hours AEL. Expression is absent in the first larval instar, resurgent in second larval instar, with a peak in the third instar that again tapers off during prepupal stages to a low level of expression in later pupal stages. Expression is high in both male and female adults, but undetectable in ovaries. The Prat2 developmental expression pattern is distinct from that of duplicate gene Prat; the latter is highly expressed in adult ovaries.

(Malmanche et al., 2003)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult heart, larval/adult fat body, adult spermathecae, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		2935.1
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		1453.8
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		2497.3
Adult InseminatedFemale Spermatheca		1365.3
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		(2935.1)
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		(814.7)
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		(1453.8)
Adult Salivary Gland		8
Adult Heart		(703.25)
Adult VirginFemale Spermatheca		(2497.3)
Adult InseminatedFemale Spermatheca		(1365.3)
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		(790.2)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		(2935.1)
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		(1453.8)
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		(2497.3)
Adult InseminatedFemale Spermatheca		(1365.3)
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		2935.1
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		1453.8
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		2497.3
Adult InseminatedFemale Spermatheca		1365.3
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		2935.1
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		1453.8
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		2497.3
Adult InseminatedFemale Spermatheca		1365.3
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		(2935.1)
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		(814.7)
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		(1453.8)
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		(2497.3)
Adult InseminatedFemale Spermatheca		(1365.3)
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		(2935.1)
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		1453.8
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		(2497.3)
Adult InseminatedFemale Spermatheca		1365.3
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.9
Larval Midgut		4.4
Larval Hindgut		17.6
Larval Malpighian Tubules		10.9
Larval Fat Body		2935.1
Larval Salivary Gland		19.9
Larval Trachea		16.875
Larval Carcass		7.75
Adult Head		814.7
Adult Eye		398.9
Adult Brain		24.3
Adult Thoracic-Abdominal Ganglion		26.3
Adult Crop		18.9
Adult Midgut		8.8
Adult Hindgut		59.1
Adult Malpighian Tubules		37.7
Adult Fat Body		1453.8
Adult Salivary Gland		8
Adult Heart		703.25
Adult VirginFemale Spermatheca		2497.3
Adult InseminatedFemale Spermatheca		1365.3
Adult Ovary		4.8
Adult Testis		10.8
Adult Male Accessory Gland		10.3
Adult Carcass		790.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	814.7
NA	Eye	398.9
NA	Brain	24.3
3.9	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	26.3
NA	Crop	18.9
4.4	Midgut	8.8
17.6	Hindgut	59.1
no informative data	Malpighian Tubules	no informative data
2935.1	Fat Body	1453.8
19.9	Salivary Gland	8
NA	Heart	703.25
16.875	Trachea	NA
NA	VirginFemale Spermatheca	2497.3
NA	InseminatedFemale Spermatheca	1365.3
NA	Ovary	4.8
NA	Testis	10.8
NA	Male Accessory Gland	no informative data
7.75	Carcass	790.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		140
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		(32)
head, virgin 20-day female		(76)
head, mated 1-day female		26
head, mated 4-day female		(73)
head, mated 20-day female		(45)
head, mated 1-day male		(39)
head, mated 4-day male		(66)
head, mated 20-day male		(76)
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		(77)
fat body, white prepupae		(140)
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		(38)
carcass, 4-day adult		(37)
carcass, 20-day adult		(45)
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		(140)
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		140
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		140
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		(76)
head, mated 1-day female		26
head, mated 4-day female		(73)
head, mated 20-day female		(45)
head, mated 1-day male		(39)
head, mated 4-day male		(66)
head, mated 20-day male		(76)
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		(77)
fat body, white prepupae		(140)
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		(38)
carcass, 4-day adult		(37)
carcass, 20-day adult		(45)
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		140
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		0
central nervous system, larvae L3		1
central nervous system, pupae P8		0
head, virgin 1-day female		22
head, virgin 4-day female		32
head, virgin 20-day female		76
head, mated 1-day female		26
head, mated 4-day female		73
head, mated 20-day female		45
head, mated 1-day male		39
head, mated 4-day male		66
head, mated 20-day male		76
salivary gland, larvae L3 wandering		2
salivary gland, white prepupae		10
digestive system, larvae L3 wandering		5
digestive system, 1-day adult		4
digestive system, 4-day adult		3
digestive system, 20-day adult		3
fat body, larvae L3 wandering		77
fat body, white prepupae		140
fat body, pupae P8		6
carcass, larvae L3 wandering		3
carcass, 1-day adult		38
carcass, 4-day adult		37
carcass, 20-day adult		45
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		2
accessory gland, mated 4-day male		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-18 hour embryonic stages, during late larval stages, during early pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		(58)
embryo 08-10hr		(62)
embryo 10-12hr		(70)
embryo 12-14hr		(57)
embryo 14-16hr		(44)
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		(37)
larva L3 puffstage 3-6		(73)
larva L3 puffstage 7-9		(74)
white prepupae new		(93)
white prepupae 12hr		(31)
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		(28)
adult male 01day		(45)
adult male 05day		(31)
adult male 30day		(30)
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		(58)
embryo 08-10hr		(62)
embryo 10-12hr		(70)
embryo 12-14hr		(57)
embryo 14-16hr		(44)
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		(37)
larva L3 puffstage 3-6		(73)
larva L3 puffstage 7-9		(74)
white prepupae new		(93)
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		(45)
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		1
embryo 02-04hr		3
embryo 04-06hr		11
embryo 06-08hr		58
embryo 08-10hr		62
embryo 10-12hr		70
embryo 12-14hr		57
embryo 14-16hr		44
embryo 16-18hr		25
embryo 18-20hr		26
embryo 20-22hr		10
embryo 22-24hr		20
larva L1		12
larva L2		5
larva L3 12hr old		20
larva L3 puffstage 1-2		37
larva L3 puffstage 3-6		73
larva L3 puffstage 7-9		74
white prepupae new		93
white prepupae 12hr		31
white prepupae 24hr		13
pupae 2d postWPP		11
pupae 3d postWPP		8
pupae 4d postWPP		28
adult male 01day		45
adult male 05day		31
adult male 30day		30
adult female 01day		23
adult female 05day		12
adult female 30day		13
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		9
cold shock, 4-day adult		6
heat shock, 4-day adult		5
Cadmium 50 mM 6 hrs, larvae L3		8
Cadmium 50 mM 12 hrs, larvae L3		20
Cadmium 50 mM 48 hrs, 4-day adult		11
Cadmium 100 mM 48 hrs, 4-day adult		14
Copper 0.5 mM 12 hrs, larvae L3		5
Copper 15 mM 48 hrs, 4-day adult		6
Zinc 5 mM 12 hrs, larvae L3		2
Zinc 4.5 mM 48 hrs, 4-day adult		11
Ethanol 2.5% 3 hrs, larvae L3		18
Ethanol 5% 3 hrs, larvae L3		5
Ethanol 10% 3 hrs, larvae L3		10
Caffeine 1.5 mg/ml 4 hrs, larvae L3		9
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		17
Caffeine 25 mg/ml 48 hrs, 4-day adult		8
Paraquat 5 mM 48 hrs, 4-day adult		6
Paraquat 10 mM 48 hrs, 4-day adult		17
Rotenone 2 μg 12 hrs, larvae L3		8
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_25

External Data & Images

Linkouts

BDGP expression data

•

GH17891

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041194

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG10078-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Prat2

Stages(s) 9-10

Stages(s) 11-12

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | partially | pupal stage, with Scer\GAL4^Act5C.PI

Prat2^{dsRNA.IR-AS.Scer\UAS}

lethal | pharate adult stage P15, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

viable, with Scer\GAL4^pnr-MD237

Prat2^GD6855

Sterility

Allele

female fertile, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

Phenotype manifest in

Allele

abdomen | P-stage, with Scer\GAL4^Act5C.PI

Prat2^{dsRNA.IR-AS.Scer\UAS}

abdomen | P-stage, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

cephalopharyngeal skeleton | P-stage, with Scer\GAL4^Act5C.PI

Prat2^{dsRNA.IR-AS.Scer\UAS}

cephalopharyngeal skeleton | P-stage, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

head | P-stage, with Scer\GAL4^Act5C.PI

Prat2^{dsRNA.IR-AS.Scer\UAS}

head | P-stage, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

leg | P-stage, with Scer\GAL4^αTub84B.PL

Prat2^{dsRNA.IR-AS.Scer\UAS}

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Prat2	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Prat2	Mutagen	Stocks	Known lesion
Prat2^GD16167	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Prat2^GD6855	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Prat2^{dsRNA.IR-AS.Scer\UAS}	in vitro construct - RNAi	0	Yes
Prat2^KK102252	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Prat2^Scer\UAS.cPa	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)Exel6109

(Ryder, 2004.4.26)

Df(3L)vvl7247-Mis12

(Sotillos et al., 2010)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

GAL4 construct

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

Evidence

References

amidophosphoribosyltransferase activity

inferred from sequence or structural similarity with UniProtKB:P28173

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

'de novo' IMP biosynthetic process

inferred from sequence or structural similarity

(FlyBase, 1992-)

nucleoside metabolic process

inferred from electronic annotation with InterPro:IPR000836

(FlyBase Curators et al., 2004-)

purine nucleobase biosynthetic process

inferred from electronic annotation with InterPro:IPR005854

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Prat2 - CG12333

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Prat2 - Hsp23

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Prat2 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

64203

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0041194

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0041194

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

38753

Orthologs

OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG60CJ2J

Dipteran inclusive ortholog search

EOG6RBQSV

Insect inclusive ortholog search

EOG6Q57JK

Arthropod inclusive ortholog search

EOG6MSBD9

Metazoa inclusive ortholog search

EOG6PP990

Orthologs in Drosophila Species (EOG60CJ2J)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10055

Drosophila willistoni

Dwil\GK12572

Drosophila virilis

Dvir\Prat2

Drosophila mojavensis

Dmoj\GI12370

Drosophila grimshawi

Dgri\GH16106

Orthologs in non-Drosophila Dipterans (EOG6RBQSV)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL003581

Anopheles gambiae

Malaria mosquito

Agam\AGAP000179

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ801629

Orthologs in non-Dipteran Insects (EOG6Q57JK)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC412619

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002769

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12214

Atta cephalotes

Leafcutter ant

Acep\ACEP17994

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO21990

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL15173

Linepithema humile

Argentine ant

Lhum\LH19615

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB24393

Solenopsis invicta

Red fire ant

Sinv\SINV18523

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001885

Bombyx mori

Silkmoth

Bmor\BGIBMGA007647

Pediculus humanus

Human body louse

Phum\PHUM430830

Tribolium castaneum

Red flour beetle

Tcas\TC016203

Tribolium castaneum

Red flour beetle

Tcas\TC000530

Orthologs in non-Insect Arthropods (EOG6MSBD9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Deer tick

Isca\ISCW015613

Orthologs in non-Arthropod Metazoa (EOG6PP990)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\T04A8.5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_003803gn

Danio rerio

Zebrafish

Drer\ppat

Xenopus tropicalis

Western clawed frog

Xtro\ppat

Gallus gallus

Domestic chicken

Ggal\PPAT

Mus musculus

House mouse

Mmus\Ppat

Rattus norvegicus

Norway rat

Rnor\Ppat

Homo sapiens

Human

Hsap\PPAT

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\PPAT

AAA Orthologs (9) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10055

Drosophila virilis

Dvir\Prat2

Drosophila mojavensis

Dmoj\GI12370

Drosophila grimshawi

Dgri\GH16106

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v48823

w¹¹¹⁸; P{GD16167}v48823

v17981

w¹¹¹⁸; P{GD6855}v17981/CyO

Genomic Clones ( 1 )

BACR14L10

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 62 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH17891

Other clones

DPiM_GH17891

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0041194

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

38753

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Amidophosphoribosyltransferase CG10078

(Clark, 2001.3.31)

Source for database merge of

Source for merge of: Prat2 Amidophosphoribosyltransferase

(Clark, 2001.8.21)

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

38753

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Class II glutamine amidotransferase domain (IPR000583)

Phosphoribosyltransferase domain (IPR000836)

Amidophosphoribosyl transferase (IPR005854)

Glutamine amidotransferase type 2 domain (IPR017932)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

GH17891

Linkouts

BioGRID - A database of protein and genetic interactions

•

64203

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0041194

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0041194

DRSC - Results from RNAi screens.

•

FBgn0041194

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0041194

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG10078-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0041194

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

38753

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

38753

modMine - Data generated by the modENCODE project.

•

FBgn0041194

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

CG10078

(Clark, 2001.3.31, Mohammad et al., 2009, Keleman et al., 2009.8.5, Cammarato et al., 2011, Malmanche et al., 2003)

DmPrat2

(Penney et al., 2008)

Prat2

(Ji and Clark, 2006, Langille and Clark, 2007, Yamamoto et al., 2009.2.25, Sotillos et al., 2010, Malmanche et al., 2003, Penney et al., 2008)

Name Synonym

Amidophosphoribosyltransferase

Phosphoribosylamidotransferase 2

(Clark, 2001.8.21, Clark and MacAfee, 2000, Cho et al., 2005, Cammarato et al., 2011)

Phosphoribosyl amidotransferase 2

(Pawar et al., 2011)

Secondary FlyBase IDs

FBgn0035717

References ( 35 )

Generate a list of

All references relating to this gene ( 35 )

List References by type

Recent research papers ( 3 )

Willoughby et al., 2013, Dis. Model Mech. 6(2): 521--529: An in vivo large-scale chemical screening platform using Drosophila for anti-cancer drug discovery. [FBrf0221096]
Weber et al., 2012, PLoS ONE 7(4): e34745: Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073]
Pawar et al., 2011, J. Biomol. Screen. 16(4): 436--442: RNAi Screening of Drosophila (Sophophora) melanogaster S2 Cells for Ricin Sensitivity and Resistance. [FBrf0213418]

Show all research papers ...