FlyBase Gene Report: Dmel\Gr43a

General Information

Symbol

Dmel\Gr43a

Species

D. melanogaster

Name

Gustatory receptor 43a

Annotation Symbol

CG1712

Feature Type

protein_coding_gene

FlyBase ID

FBgn0041243

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Gustatory receptor which mediates acceptance or avoidance behavior, depending on its substrates. Gr43a is the main sugar receptor in larvae. Functions as a narrowly tuned fructose receptor in taste neurons but also as a fructose receptor in the brain. Necessary and sufficient to sense hemolymph fructose and promote feeding in hungry flies but suppress feeding in satiated flies. (UniProt, Q9V4K2)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Gr43C1

Key Links

Genomic Location

Cytogenetic map

43B2-43B2

Sequence location

2R:7,390,869..7,393,161 [-]

Recombination map

2-56

RefSeq locus

NT_033778 REGION:7390869..7393161

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sweet taste receptor activity

inferred from mutant phenotype

(Freeman et al., 2014, Miyamoto et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables taste receptor activity

non-traceable author statement

(assigned by UniProt )

(Dunipace et al., 2001, Clyne et al., 2000)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in adult feeding behavior

inferred from mutant phenotype

(Miyamoto et al., 2012)

involved_in cellular response to fructose stimulus

inferred from mutant phenotype

(Miyamoto et al., 2012)

involved_in regulation of appetite

inferred from mutant phenotype

(Miyamoto et al., 2012)

involved_in regulation of feeding behavior

inferred from mutant phenotype

(Miyamoto et al., 2012)

involved_in sensory perception of sweet taste

inferred from mutant phenotype

(Freeman et al., 2014, Mishra et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in sensory perception of taste

non-traceable author statement

(assigned by UniProt )

(Dunipace et al., 2001, Clyne et al., 2000)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in axon

inferred from biological aspect of ancestor with PANTHER:PTN000475107

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in dendrite

inferred from biological aspect of ancestor with PANTHER:PTN000475107

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in integral component of membrane

inferred from electronic annotation with InterPro:IPR013604

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

non-traceable author statement

(assigned by UniProt )

(Dunipace et al., 2001, Clyne et al., 2000)

is_active_in neuronal cell body

inferred from biological aspect of ancestor with PANTHER:PTN000475107

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

GUSTATORY RECEPTORS

Protein Family (UniProt)

Belongs to the insect chemoreceptor superfamily. Gustatory receptor (GR) family. Gr21a subfamily. (Q9V4K2)

Protein Signatures (InterPro)

7TM chemoreceptor

Summaries

Gene Group (FlyBase)

GUSTATORY RECEPTORS -: The Gustatory receptors are a divergent group of (non-GPCR) seven-transmembrane chemoreceptors that are expressed in gustatory sensilla receptor neurons. They detect tastants (non-volatile compounds) via contact chemosensation. (Adapted from FBrf0208984).

Protein Function (UniProtKB)

(UniProt, Q9V4K2)

Summary (Interactive Fly)

nutrient sensor for hemolymph fructose - promotes feeding in hungry flies but suppresses feeding in satiated flies

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gr43a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V4K2)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.48

Annotation based on personal communication from Hugh Robertson (FBrf0159825).

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gr43a-RA

FBtr0089014

NM_078926

1449

427

Gr43a-RB

FBtr0110820

NM_001043066

1515

449

Gr43a-RC

FBtr0344883

NM_001299229

1527

453

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Gr43a-PA

FBpp0088086

47.6

427

9.65

NP_523650

AAF59268

Gr43a-PB

FBpp0110117

49.9

449

9.71

NP_001036531

ABI31082

Gr43a-PC

FBpp0311196

50.4

453

9.79

NP_001286158

AHN55956

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

7TM chemoreceptor

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gr43a using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gr43a_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Gr43a-GAL4.0.5}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

gustatory receptor neuron of the epiphysis

(Kwon et al., 2011)

larval gnathal ganglion

(Kwon et al., 2011)

larval gustatory receptor neuron of the epiphysis DP4

(Choi et al., 2016)

Reporter: P{Gr43a-GAL4.5.3}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

gustatory receptor neuron of the epiphysis

(Kwon et al., 2011)

larval gnathal ganglion

(Kwon et al., 2011)

adult stage

sugar-sensing neuron of taste bristle | subset of prothoracic tarsal segment 5

(Ling et al., 2014)

sugar-sensing neuron of taste bristle | subset of mesothoracic tarsal segment 5

(Ling et al., 2014)

sugar-sensing neuron of taste bristle | subset of metathoracic tarsal segment 5

(Ling et al., 2014)

Reporter: P{Gr43a-lexA::VP16}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

prothoracic leg taste bristle chemosensory neuron | subset of tarsal segment 5

(Fujii et al., 2015)

sugar-sensing neuron of taste bristle | subset of prothoracic leg taste bristle chemosensory neuron

(Fujii et al., 2015)

posterior superior lateral protocerebrum | restricted

(Fujii et al., 2015)

adult brain | restricted

(Fujii et al., 2015)

Reporter: TI{GAL4}Gr43a^GAL4

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Gr43a in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gr43a

View exonic expression by tissue for Dmel\Gr43a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041243

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35655/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041243

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041243

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041243

Flygut - An atlas of the Drosophila adult midgut

FBgn0041243

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gr43a

Transgenic constructs containing regulatory region of Gr43a

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gr43a^GD599

viable, with Scer\GAL4^Mef2.PR

Gr43a^GD599

viable, with Scer\GAL4^pnr-MD237

Gr43a^GD599

Other Phenotypes

Allele

abnormal feeding behavior

Gr43a^GAL4

abnormal taste perception, with Scer\GAL4^Gr21a.9.323, Scer\GAL4^Gr63a.PF

Gr43a^UAS.cFa

Phenotype manifest in

Allele

prothoracic leg taste bristle chemosensory neuron

Gr43a^GAL4

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (0)

No records found.

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (0)

No records found.

Danio rerio (Zebrafish) (0)

No records found.

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\egl-47

1 of 15

Yes

Cele\gur-3

1 of 15

Yes

Cele\lite-1

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (47)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gr61a

enhanceable

Gr43a

(Miyamoto et al., 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0041243

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-56

Cytogenetic map

43B2-43B2

Sequence location

2R:7,390,869..7,393,161 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43B2-43B2

Limits computationally determined from genome sequence between P{lacW}cos^k16101 and P{EP}CG1600^EP398

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-56

(FlyBase, 2016)

2-56.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93448

w¹¹¹⁸; P{UAS-Gr43a.M}3

93447

w¹¹¹⁸; TI{GAL4}Gr43a^GAL4

57636

w^*; P{Gr43a-GAL4.0.5}9; Dr¹/TM3, Sb¹

83280

y¹ w^*; TI{CRIMIC.TG4.2}Gr43a^{CR01353-TG4.2}

64881

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05754}attP40

VDRC

v107681

P{KK113182}VIE-260B

v39518

w¹¹¹⁸; P{GD599}v39518

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (9)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0041243

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0041243

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35655

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Gr43a functions as a fructose receptor in taste neurons, and also in the brain. It is both necessary and sufficient to sense hemolymph fructose and promote feeding in hungry flies but suppress feeding in satiated flies.

(Miyamoto et al., 2012)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 38 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35655

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V4K2

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V4K2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V4K2

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041243

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0041243

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35655/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041243

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gr43a_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041243

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041243

Flygut - An atlas of the Drosophila adult midgut

FBgn0041243

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35655

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

7TM chemoreceptor

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1712

MARRVEL_MODEL - MARRVEL (model organism gene)

35655

modMine - A data warehouse for the modENCODE project

FBgn0041243

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0041243

DRSC - Results frm RNAi screens

FBgn0041243

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0041243

FlyMine - An integrated database for Drosophila genomics

FBgn0041243

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

43C.1

(Clyne et al., 2000)

43C1

(Dunipace et al., 2001)

CG1712

(Yurgel et al., 2019, Keleman et al., 2009.8.5, Perkins et al., 2009, Robertson, 2003.6.18, Dunipace et al., 2001)

DmGr43a

(Robertson, 2005.7.22)

GR43C.1

GR43a

(Pavin et al., 2018, Miyamoto et al., 2013, Montell, 2013, Montell, 2009)

Gr43C1

(Scott and Axel, 2001.4.25, Scott et al., 2001)

gr43a

(Riveron et al., 2013)

Name Synonyms

Gustatory receptor 43C.1

(Clyne et al., 2000)

Gustatory receptor 43a

(Robertson, 2001.11.11)

Secondary FlyBase IDs

FBgn0033161

Datasets (7)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (7)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (82)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections