olf186-F, dOrai, CRACM1, l(2)k11505
store-operated Ca channel involved in rhythmic firing of the flight motoneurons - physically interacts with the Ca store sensor Stim (Stromal interaction molecule) to form the Ca release-activated Ca2+ (CRAC) channel
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.48
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Orai using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Orai in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Orai olf186-F
S2R+ cells treated with dsRNA made from templates generated with primers against this gene show complete inhibition of thapsigargin-induced Ca2+ influx, but there is no effect on the filling state of Ca2+ stores.
Treatment of S2 cells with dsRNA made from templates generated with primers directed against this gene inhibits both the early and sustained components of Ca2+ entry evoked by thapsigargin at the single-cell level. In the majority of treated cells, Ca2+ release-activated Ca2+ current is completely suppressed.
The gene is named "Orai" - in Greek mythology, the Orai are keepers of the gates of heaven: Eunomia (Order or Harmony), Dike (Justice) and Eirene (Peace).
Named 'Orai' because, in Greek mythology, the Orai are the keepers of the gates of heaven, and the Orai protein is an essential component of the store-operated Ca[2+] entry mechanism.