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Gene Dmel\rho-5

General Information
Symbol	Dmel\rho-5	Species	D. melanogaster
Name	rhomboid-5	Annotation symbol	CG33304
Feature type	protein_coding_gene	FlyBase ID	FBgn0041723
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	31D10-31D11	Sequence location	2L:10,368,134..10,373,900 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene rhomboid-5 is referred to in FlyBase by the symbol Dmel\rho-5 (CG33304, FBgn0041723). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: serine-type endopeptidase activity. Based on sequence similarity, it is predicted to be involved in the biological process: signal transduction. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase S54, rhomboid; Peptidase S54, rhomboid domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1629010_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of rho-5. Gene sequence location is 2L:10368134..10373900.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Zettl et al., 2011, Cell 145(1): 79--91 Sequence features BKN33275 HFA02847
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 31D10-31D11   Limits computationally determined from genome sequence between P{lacW}RnrLk06709 and P{lacW}KdelRk00311
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\rho-5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene split based on BLASTX data
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) rho-5-RA FBtr0089855  NM_205957   4576   1429
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein rho-5-PA   FBpp0088796   157.3   1429   8.29     NP_995679   AAS64674
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S54, rhomboid (IPR002610) Peptidase S54, rhomboid domain (IPR022764)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA699010   AE014134 AAS64674   BT011096   BT125661   CO186975   CO274860   CO278532   CO282741   CO295766   CO326349   CO330041   CO331436   CO331928   CO340470   CZ466816   CZ467180   CZ467181   CZ471097   CZ471249   EC068243   EC205122   EC210017   EC212870   EC216276   EC217044   EC228150   EC245488   EC255850   EC259023   EW712792   EW712916
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q76NQ1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0041723    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0041723 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 12-18 embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0041723 expression level Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr  (175) embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr  (142) embryo 10-12hr  (625) embryo 12-14hr  (692) embryo 14-16hr  (1507) embryo 16-18hr  (1292) embryo 18-20hr  (1295) embryo 20-22hr  (867) embryo 22-24hr  (796) larva L1  (884) larva L2  (252) larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6  (221) larva L3 puffstage 7-9  (269) white prepupae new  (343) white prepupae 12hr  (644) white prepupae 24hr  (849) pupae 2d postWPP  (744) pupae 3d postWPP  (610) pupae 4d postWPP  (516) adult male 01day  (360) adult male 05day  (415) adult male 30day  (494) adult female 01day  (307) adult female 05day  106 adult female 30day  (120) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr  1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0041723 expression level Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr  (175) embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr  142 embryo 10-12hr  (625) embryo 12-14hr  (692) embryo 14-16hr  (1507) embryo 16-18hr  (1292) embryo 18-20hr  (1295) embryo 20-22hr  (867) embryo 22-24hr  (796) larva L1  (884) larva L2  (252) larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6  (221) larva L3 puffstage 7-9  (269) white prepupae new  (343) white prepupae 12hr  (644) white prepupae 24hr  (849) pupae 2d postWPP  (744) pupae 3d postWPP  (610) pupae 4d postWPP  (516) adult male 01day  (360) adult male 05day  (415) adult male 30day  (494) adult female 01day  (307) adult female 05day  106 adult female 30day  120 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr  1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    96 embryo 02-04hr    175 embryo 04-06hr    74 embryo 06-08hr    82 embryo 08-10hr    142 embryo 10-12hr    625 embryo 12-14hr    692 embryo 14-16hr    1507 embryo 16-18hr    1292 embryo 18-20hr    1295 embryo 20-22hr    867 embryo 22-24hr    796 larva L1    884 larva L2    252 larva L3 12hr old    73 larva L3 puffstage 1-2    94 larva L3 puffstage 3-6    221 larva L3 puffstage 7-9    269 white prepupae new    343 white prepupae 12hr    644 white prepupae 24hr    849 pupae 2d postWPP    744 pupae 3d postWPP    610 pupae 4d postWPP    516 adult male 01day    360 adult male 05day    415 adult male 30day    494 adult female 01day    307 adult female 05day    106 adult female 30day    120 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0041723    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0041723 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0041723 expression level Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0041723 expression level Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    242.075 Larval Midgut    8.4 Larval Hindgut    5.1 Larval Malpighian Tubules    4.7 Larval Fat Body    3.2 Larval Salivary Gland    6.9 Larval Trachea    8.7 Larval Carcass    5.075 Adult Head    100.2 Adult Eye    97.325 Adult Brain    334 Adult Thoracic-Abdominal Ganglion    269.9 Adult Crop    4.4 Adult Midgut    6.5 Adult Hindgut    5.3 Adult Malpighian Tubules    4.4 Adult Fat Body    6.4 Adult Salivary Gland    2.5 Adult Heart    4.1 Adult VirginFemale Spermatheca    4.5 Adult InseminatedFemale Spermatheca    3.3 Adult Ovary    9 Adult Testis    9.6 Adult Male Accessory Gland    4.7 Adult Carcass    9.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    100.2   NA  Eye    97.325   NA  Brain    334   242.075  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    269.9   NA  Crop    4.4   8.4  Midgut    6.5   5.1  Hindgut    5.3   4.7  Malpighian Tubules    4.4   3.2  Fat Body    6.4   6.9  Salivary Gland    2.5   NA  Heart    4.1   8.7  Trachea    NA   NA  VirginFemale Spermatheca    4.5   NA  InseminatedFemale Spermatheca    3.3   NA  Ovary    9   NA  Testis    9.6   NA  Male Accessory Gland    4.7   5.075  Carcass    9.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_29
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041723 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5364-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 rho-5GD1064 viable rho-5c01455 Sterility Allele fertile rho-5c01455 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of rho-5 Class Mutagen Stocks Known lesion rho-5c01455 piggyBac transposase 2 -- rho-5c01023 piggyBac transposase 1 -- rho-5c06935 piggyBac transposase 0 -- rho-5c07113 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of rho-5 Class Mutagen Stocks Known lesion rho-5GD1064 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes rho-5KK113275 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1064} NA P{KK113275} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}rho-5c01023 NA PBac{PB}rho-5c01455 NA PBac{PB}rho-5c06935 NA PBac{PB}rho-5c07113 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
serine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR002610, InterPro:IPR022764 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
proteolysis inferred from electronic annotation with InterPro:IPR002610 (FlyBase Curators et al., 2004-) signal transduction inferred from sequence or structural similarity with EMBL:BC023469 (FlyBase Genome Annotators, 2004)
Cellular Component
CV term Evidence References
integral to membrane inferred from sequence or structural similarity with EMBL:BC023469 (FlyBase Genome Annotators, 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions rho-5 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 77793 DroID - A comprehensive database of gene and protein interactions. • FBgn0041723 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 2768944
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14105 Dere\GG23940 Dgri\GH23944 Dmoj\GI14027 Dper\GL19157 Dpse\GA27747 Dsec\GM11703 Dsim\GD22266 Dwil\GK10435 Dyak\GE26031
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL000817-PA Aedes aegypti (mosquito) XP_395087.3 Apis mellifera (honey bee) ENSBTAP00000026388 Bos taurus (bovine) CN18038 Caenorhabditis brenneri (small nematode) CE40475 Caenorhabditis elegans (worm) ENSCAFP00000029172 Canis familiaris (dog) JA09050 Caenorhabditis japonica (nematode) CPIJ013320 Culex pipens (mosquito) ENSDARP00000040908 Danio rerio (zebra fish) ENSECAP00000020889 Equus caballus (horse) 194219 Hypsipyla robusta (cedar shoot borer) 27089 Monocosta brevicollis (choanoflagellate) ENSMODP00000020743 Monodelphis domestica (gray opossum) ENSMUSP00000020524 Mus musculus (house mouse) XP_001605580.1 Nasonia vitripennis (wasp) ENSOANP00000018324 Ornithorynchus anatinus (platypus) ENSORLP00000000928 Oryzias latipes (japanese killifish) PHUM004153-PA Pediculus humanus (human body louse) ENSPTRP00000016460 Pan troglodytes (chimpanzee) ENSRNOP00000027965 Rattus norvegicus (norway rat) XP_970266.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020659 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000054037 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14713 PB14714 NV15356 LH20586 ISCW023071 FBgn0243074 FBgn0212450 FBgn0212448 FBgn0193674 FBgn0166646 JGI_V11_55210 FBgn0249111 FBgn0156756 FBgn0136781 FBgn0131401 FBgn0116079 FBgn0091132 CPIJ013320 CPIJ017919 BGIBMGA007277 ACYPI21777 ACYPI003334 GB19349 GB15260 AGAP013190 AAEL004678 AAEL000817 PHUM325470 GLEAN_06551
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	10540 w1118; PBac{PB}rho-5c01455
Harvard	- PBac{PB}rho-5c01023 - PBac{PB}rho-5c01455
VDRC	v108514 P{KK113275}VIE-260B v2781 w1118; P{GD1064}v2781/CyO
Genomic Clones ( 2 )
	BACR30M19 BACR48K04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 22 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE30726
Other clones	MIP25159
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL06695
Other clones	17598 45520 78014 191950 272874 274606 CK01.113D.A6 EC255850 EC259023 EK025106 EK115804 EK130313 EK161119 EK211539 EK294419 EK299739 EK302035 EP03724 EP10718
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0041723 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34407
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: rhomboid-5 CG5364 (Wasserman et al., 2000)
Additional comments	Annotation CG5364 split into CG33303 and CG33304 (which corresponds to rho-5) in release 3.2 of the genome annotation. (FlyBase Genome Annotators, 2004)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_205957 RefSeq (Proteins) • NP_995679 DDBJ / EMBL / GenBank • AAS64674 CZ466816 CZ467180 CZ467181 CZ471097 CZ471249 Entrez Gene - A searchable database of RefSeq genes. • 2768944 UniProtKB/TrEMBL • Q76NQ1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase S54, rhomboid (IPR002610) Peptidase S54, rhomboid domain (IPR022764)
Linkouts
	BioGRID - A database of protein and genetic interactions • 77793 DroID - A comprehensive database of gene and protein interactions. • FBgn0041723 DRSC - Results from RNAi screens. • FBgn0041723 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0041723 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5364-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0041723 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34407 InParanoid A subset of ortholog calls from InParanoid. • AAEL000817-PA Aedes aegypti (mosquito) XP_395087.3 Apis mellifera (honey bee) ENSBTAP00000026388 Bos taurus (bovine) CN18038 Caenorhabditis brenneri (small nematode) CE40475 Caenorhabditis elegans (worm) ENSCAFP00000029172 Canis familiaris (dog) JA09050 Caenorhabditis japonica (nematode) CPIJ013320 Culex pipens (mosquito) ENSDARP00000040908 Danio rerio (zebra fish) ENSECAP00000020889 Equus caballus (horse) 194219 Hypsipyla robusta (cedar shoot borer) 27089 Monocosta brevicollis (choanoflagellate) ENSMODP00000020743 Monodelphis domestica (gray opossum) ENSMUSP00000020524 Mus musculus (house mouse) XP_001605580.1 Nasonia vitripennis (wasp) ENSOANP00000018324 Ornithorynchus anatinus (platypus) ENSORLP00000000928 Oryzias latipes (japanese killifish) PHUM004153-PA Pediculus humanus (human body louse) ENSPTRP00000016460 Pan troglodytes (chimpanzee) ENSRNOP00000027965 Rattus norvegicus (norway rat) XP_970266.1 Tribolium castaneum (red flour beetle) ENSTNIP00000020659 Tetraodon nigroviridis (green spotted pufferfish) ENSXETP00000054037 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 2768944 modMine - Data generated by the modENCODE project. • FBgn0041723 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14713 PB14714 NV15356 LH20586 ISCW023071 FBgn0243074 FBgn0212450 FBgn0212448 FBgn0193674 FBgn0166646 JGI_V11_55210 FBgn0249111 FBgn0156756 FBgn0136781 FBgn0131401 FBgn0116079 FBgn0091132 CPIJ013320 CPIJ017919 BGIBMGA007277 ACYPI21777 ACYPI003334 GB19349 GB15260 AGAP013190 AAEL004678 AAEL000817 PHUM325470 GLEAN_06551
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG5364 (Giot et al., 2003, ) CG33304 (Keleman et al., 2009.8.5) CT17046 (Wasserman et al., 2000) rho-5 (Zeitlinger et al., 2007, Leung et al., 2010) Rho-related31D10 (Guichard et al., 2000)
Name Synonym	rhomboid-5 (Wasserman et al., 2000)
Secondary FlyBase IDs
	FBgn0032232 FBgn0053304
References ( 19 )
Research paper	Zettl et al., 2011, Cell 145(1): 79--91 Rhomboid Family Pseudoproteases Use the ER Quality Control Machinery to Regulate Intercellular Signaling. [FBrf0213387] Leung et al., 2010, Genetics 185(4): 1519--1534 Evolution of a Distinct Genomic Domain in Drosophila: Comparative Analysis of the Dot Chromosome in Drosophila melanogaster and Drosophila virilis. [FBrf0211495] Guichard et al., 2000, Dev. Biol. 226(2): 255--266 brother of rhomboid, a rhomboid-related gene expressed during early Drosophila oogenesis, promotes EGF-R/MAPK signaling. [FBrf0129841] Wasserman et al., 2000, Genes Dev. 14(13): 1651--1663 A family of rhomboid-like genes: Drosophila rhomboid-1 and roughoid/rhomboid-3 cooperate to activate EGF receptor signaling. [FBrf0128677]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Zeitlinger et al., 2007, Nat. Genet. 39(12): Supplementary Information [FBrf0208880] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Review	Klambt, 2000, Curr. Biol. 10(10): R388--R391 EGF receptor signalling: the importance of presentation. [FBrf0128528]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase Genome Annotators, 2004, Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. Changes affecting gene model number or type in release 3.2 of the annotated D.melanogaster genome. [FBrf0166453]
Computer file	Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection. Genomic mapping of Exelixis insertion collection. [FBrf0184340] Gene Disruption Project members, 2001-, [title not yet available] [title not yet available] [FBrf0132177]
Curated genome annotation	Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126651]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]